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The map label for this gene is afr [H]

Identifier: 209549685

GI number: 209549685

Start: 2151643

End: 2152776

Strand: Reverse

Name: afr [H]

Synonym: Rleg2_2092

Alternate gene names: 209549685

Gene position: 2152776-2151643 (Counterclockwise)

Preceding gene: 209549686

Following gene: 209549684

Centisome position: 47.44

GC content: 64.11

Gene sequence:

>1134_bases
ATGACCAGGGAACTTGGCGTCGGCATCATTGGATGCGGCAACATCTCCACCACCTACTTTTCGCTGGCACCGCTCTTCAA
GGGGCTGAAGGTGCTGGCTTGCGCCGATATCAACGTCCAGGCGGCCGAGGCGCGCGCCAAAGAATATGGCGTGAAGGCGC
AGACGATCGACGCGCTTCTCGCCAATGACGAGATCGACGTCGTCGTCAACCTGACGATCCCGGATGCGCATTTCCGCGTG
TCGAAGTCGATCCTCGAGGCCGGAAAACACGTCTATTCCGAAAAGCCGCTGGTGCTTTCGCTTGAGGAGGGCGAAGAGCT
TCGCCGCATCGCGAGGGAAAAGAACCTGGCGGTCGGCTGCGCGCCGGACACTTTCCTCGGCGGCGCCCATCAGCTCGCCC
GCAAGTTCATCGATGACGGCGGCATCGGCCGGGTGACGTCGGGCGCCTGCTACGTGATGAGCCCCGGCATGGAGATGTGG
CATCCGAACCCGGATTTCTTCTTCCTGCCGGGCGGCGGCCCGATCCTCGATCTCGGCCCCTATTACATCGCCAATCTGAT
CAACCTGATCGGTCCGGTGAAACGCGTCGGCGCCATGACCTCGATGGCGTCGCCGACCCGCACCATCACCAGCGAGCCGC
GCAACGGCGAGATCATCCCGGTCAAGACGCCGACGACGATCCAGGCGCTGCTCGAATTCGTCAGCGGCGCCACCGTGACG
CTGTCGGCAAGCTGGGACGTCTGGTCGCATCGCCATGCCAATATGGAGCTCTACGGCACCGGTGGCTCGCTCTACGTGCC
GGATCCGAACTTCTTCGGCGGCACGGTCGAGGCAAGCGGCCGCGACAAGGACATCAAGCCGCTCGACGCCTGGGAGCATC
CGTTCGGTAAGATCAACCAGGAAAACCCGAACGGATCGCGCGCCAACTACCGCACGGCCGGCCTTGCCGACATGGCGATG
TCGCTGATCGAAGGCCGCGACGCCCGCTGCTCGCTCGACCGCACGCTGCACGGGGTCGACGTCATGACCTCGATACTGAA
GTCGGGCGAGGAGGGCCGTTTCATCGACCTGACCACGACCTGCACGCAGCCGGCAGCGCTCGGCATCGAGGAGGCGCAGG
CGCTGTTGAAGTGA

Upstream 100 bases:

>100_bases
CCAAGGCGAAGCACTTCGTCGTCGAACATGATAATCCCAAAGACATCGACCGCAACATCAGCCGTTCGATTGCATCCTTC
CAGACTTATTGAGGCACATC

Downstream 100 bases:

>100_bases
TAGGATAGCGTTGGAATCGTTGGGCTGAGAATTGCCCCTCACCCTAACCCTCTCCCCGCAAACGGGGAGAGGGGGCGAGC
CAAACGCAGCGCCGAGATTT

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MTRELGVGIIGCGNISTTYFSLAPLFKGLKVLACADINVQAAEARAKEYGVKAQTIDALLANDEIDVVVNLTIPDAHFRV
SKSILEAGKHVYSEKPLVLSLEEGEELRRIAREKNLAVGCAPDTFLGGAHQLARKFIDDGGIGRVTSGACYVMSPGMEMW
HPNPDFFFLPGGGPILDLGPYYIANLINLIGPVKRVGAMTSMASPTRTITSEPRNGEIIPVKTPTTIQALLEFVSGATVT
LSASWDVWSHRHANMELYGTGGSLYVPDPNFFGGTVEASGRDKDIKPLDAWEHPFGKINQENPNGSRANYRTAGLADMAM
SLIEGRDARCSLDRTLHGVDVMTSILKSGEEGRFIDLTTTCTQPAALGIEEAQALLK

Sequences:

>Translated_377_residues
MTRELGVGIIGCGNISTTYFSLAPLFKGLKVLACADINVQAAEARAKEYGVKAQTIDALLANDEIDVVVNLTIPDAHFRV
SKSILEAGKHVYSEKPLVLSLEEGEELRRIAREKNLAVGCAPDTFLGGAHQLARKFIDDGGIGRVTSGACYVMSPGMEMW
HPNPDFFFLPGGGPILDLGPYYIANLINLIGPVKRVGAMTSMASPTRTITSEPRNGEIIPVKTPTTIQALLEFVSGATVT
LSASWDVWSHRHANMELYGTGGSLYVPDPNFFGGTVEASGRDKDIKPLDAWEHPFGKINQENPNGSRANYRTAGLADMAM
SLIEGRDARCSLDRTLHGVDVMTSILKSGEEGRFIDLTTTCTQPAALGIEEAQALLK
>Mature_376_residues
TRELGVGIIGCGNISTTYFSLAPLFKGLKVLACADINVQAAEARAKEYGVKAQTIDALLANDEIDVVVNLTIPDAHFRVS
KSILEAGKHVYSEKPLVLSLEEGEELRRIAREKNLAVGCAPDTFLGGAHQLARKFIDDGGIGRVTSGACYVMSPGMEMWH
PNPDFFFLPGGGPILDLGPYYIANLINLIGPVKRVGAMTSMASPTRTITSEPRNGEIIPVKTPTTIQALLEFVSGATVTL
SASWDVWSHRHANMELYGTGGSLYVPDPNFFGGTVEASGRDKDIKPLDAWEHPFGKINQENPNGSRANYRTAGLADMAMS
LIEGRDARCSLDRTLHGVDVMTSILKSGEEGRFIDLTTTCTQPAALGIEEAQALLK

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 40593; Mature: 40462

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRELGVGIIGCGNISTTYFSLAPLFKGLKVLACADINVQAAEARAKEYGVKAQTIDALL
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEHHHHHHHHHCCCHHHHHHHH
ANDEIDVVVNLTIPDAHFRVSKSILEAGKHVYSEKPLVLSLEEGEELRRIAREKNLAVGC
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEC
APDTFLGGAHQLARKFIDDGGIGRVTSGACYVMSPGMEMWHPNPDFFFLPGGGPILDLGP
CCHHHCCCHHHHHHHHHCCCCCCEECCCEEEEECCCCHHCCCCCCEEEECCCCCEEECCH
YYIANLINLIGPVKRVGAMTSMASPTRTITSEPRNGEIIPVKTPTTIQALLEFVSGATVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEE
LSASWDVWSHRHANMELYGTGGSLYVPDPNFFGGTVEASGRDKDIKPLDAWEHPFGKINQ
EEECCCCCCCCCCCEEEEECCCEEECCCCCCCCCEEECCCCCCCCCCCCHHCCCCCCCCC
ENPNGSRANYRTAGLADMAMSLIEGRDARCSLDRTLHGVDVMTSILKSGEEGRFIDLTTT
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
CTQPAALGIEEAQALLK
CCCCHHCCHHHHHHHCC
>Mature Secondary Structure 
TRELGVGIIGCGNISTTYFSLAPLFKGLKVLACADINVQAAEARAKEYGVKAQTIDALL
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCEEHHHHHHHHHCCCHHHHHHHH
ANDEIDVVVNLTIPDAHFRVSKSILEAGKHVYSEKPLVLSLEEGEELRRIAREKNLAVGC
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEC
APDTFLGGAHQLARKFIDDGGIGRVTSGACYVMSPGMEMWHPNPDFFFLPGGGPILDLGP
CCHHHCCCHHHHHHHHHCCCCCCEECCCEEEEECCCCHHCCCCCCEEEECCCCCEEECCH
YYIANLINLIGPVKRVGAMTSMASPTRTITSEPRNGEIIPVKTPTTIQALLEFVSGATVT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEE
LSASWDVWSHRHANMELYGTGGSLYVPDPNFFGGTVEASGRDKDIKPLDAWEHPFGKINQ
EEECCCCCCCCCCCEEEEECCCEEECCCCCCCCCEEECCCCCCCCCCCCHHCCCCCCCCC
ENPNGSRANYRTAGLADMAMSLIEGRDARCSLDRTLHGVDVMTSILKSGEEGRFIDLTTT
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEC
CTQPAALGIEEAQALLK
CCCCHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA