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The map label for this gene is artJ [H]

Identifier: 209549649

GI number: 209549649

Start: 2121555

End: 2122328

Strand: Direct

Name: artJ [H]

Synonym: Rleg2_2056

Alternate gene names: 209549649

Gene position: 2121555-2122328 (Clockwise)

Preceding gene: 209549647

Following gene: 209549650

Centisome position: 46.75

GC content: 59.17

Gene sequence:

>774_bases
ATGCATATCTCCACCCGTATTTTCGCGGCAGCCTCGATCGCGGCGATGTCCCTTTTCGCCGGCTCGGCCATGGCCGATGG
CGAGAAGTATGTGATCGGCACCGATTCGACCTATCCGCCCTTCGAGTTCGTCGATGCCAGCGGCACGATCCAGGGCTTCG
ACATCGACATCACGAAGGCGCTTTGTGCCGAGATGAAGGCTGAATGCTCCTTCGTCAGCACCGACTGGGACGGCATCATC
CCGGCGCTCAACGCCAAGAAGTTCGACATGATCGTTTCCTCCATGTCGATCACGCCGGAGCGCCTGAAGCTCGTCGACTT
CTCCAACAAGTACTACAACACCCCGCCGGCCATCGCCGTGCCGAAGGATTCGAAGATCACCGACGTTGCCGGCCTCAAAG
GCAAGGTGATCGGCGCACAGACCTCGACAACGCACGCCAACTACGCCGAGAAGCACCTGGCCGACACCGAACTGAAGCTC
TATCCGACGGCTGACGAATACAAGCTCGACGTCTCCAGCGGCCGTGTCGACGCCGTCATCGACGACGTCGTCGTTCTCTC
CGAATGGGTGAAATCCGACGCCGGCGCCTGCTGCAAGATCCTGACCACCCTGCCGGTCGACAAGGAAATCAACGGCAACG
GCGCAGGCATTGCCATCCGTAAAGGCGATCCGCTCAAGGAAAAGCTGAACACGGCAATCGCTGCGATCCGCGCCAGCGGT
GAATACAAGAAGATCCAGGACAAGTACTTCGACTTCGACGTTTACGGCGAATAA

Upstream 100 bases:

>100_bases
AAAAAAGTCTGATTATCGCCGCAAGGCGGTCTCGGACCGTTGAACTGCAGCCGTCAGCCCGCGAGAAAAAGAGCGGCGGC
TACCAACAGAGAGGATCCTC

Downstream 100 bases:

>100_bases
GAAATTCGCTATCTGGCCAGTCAAGGTAATGGCGGAAGGCTTGCTCTTCCGCCATTTTTGTTTGACAAAGATGGCAAGAT
CAAAACGGCAAAAGCCGTGA

Product: family 3 extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MHISTRIFAAASIAAMSLFAGSAMADGEKYVIGTDSTYPPFEFVDASGTIQGFDIDITKALCAEMKAECSFVSTDWDGII
PALNAKKFDMIVSSMSITPERLKLVDFSNKYYNTPPAIAVPKDSKITDVAGLKGKVIGAQTSTTHANYAEKHLADTELKL
YPTADEYKLDVSSGRVDAVIDDVVVLSEWVKSDAGACCKILTTLPVDKEINGNGAGIAIRKGDPLKEKLNTAIAAIRASG
EYKKIQDKYFDFDVYGE

Sequences:

>Translated_257_residues
MHISTRIFAAASIAAMSLFAGSAMADGEKYVIGTDSTYPPFEFVDASGTIQGFDIDITKALCAEMKAECSFVSTDWDGII
PALNAKKFDMIVSSMSITPERLKLVDFSNKYYNTPPAIAVPKDSKITDVAGLKGKVIGAQTSTTHANYAEKHLADTELKL
YPTADEYKLDVSSGRVDAVIDDVVVLSEWVKSDAGACCKILTTLPVDKEINGNGAGIAIRKGDPLKEKLNTAIAAIRASG
EYKKIQDKYFDFDVYGE
>Mature_257_residues
MHISTRIFAAASIAAMSLFAGSAMADGEKYVIGTDSTYPPFEFVDASGTIQGFDIDITKALCAEMKAECSFVSTDWDGII
PALNAKKFDMIVSSMSITPERLKLVDFSNKYYNTPPAIAVPKDSKITDVAGLKGKVIGAQTSTTHANYAEKHLADTELKL
YPTADEYKLDVSSGRVDAVIDDVVVLSEWVKSDAGACCKILTTLPVDKEINGNGAGIAIRKGDPLKEKLNTAIAAIRASG
EYKKIQDKYFDFDVYGE

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=246, Percent_Identity=39.4308943089431, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1787088, Length=250, Percent_Identity=38.4, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1788648, Length=230, Percent_Identity=40.8695652173913, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=37.109375, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1787031, Length=242, Percent_Identity=32.2314049586777, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788228, Length=258, Percent_Identity=28.6821705426357, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27733; Mature: 27733

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISTRIFAAASIAAMSLFAGSAMADGEKYVIGTDSTYPPFEFVDASGTIQGFDIDITKA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCEEEEEEHHHHH
LCAEMKAECSFVSTDWDGIIPALNAKKFDMIVSSMSITPERLKLVDFSNKYYNTPPAIAV
HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEE
PKDSKITDVAGLKGKVIGAQTSTTHANYAEKHLADTELKLYPTADEYKLDVSSGRVDAVI
CCCCCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHH
DDVVVLSEWVKSDAGACCKILTTLPVDKEINGNGAGIAIRKGDPLKEKLNTAIAAIRASG
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
EYKKIQDKYFDFDVYGE
CHHHHHHHHCCEEECCC
>Mature Secondary Structure
MHISTRIFAAASIAAMSLFAGSAMADGEKYVIGTDSTYPPFEFVDASGTIQGFDIDITKA
CCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCEEEEEEHHHHH
LCAEMKAECSFVSTDWDGIIPALNAKKFDMIVSSMSITPERLKLVDFSNKYYNTPPAIAV
HHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCEEE
PKDSKITDVAGLKGKVIGAQTSTTHANYAEKHLADTELKLYPTADEYKLDVSSGRVDAVI
CCCCCCEEECCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCEEEECCCCCCHHHH
DDVVVLSEWVKSDAGACCKILTTLPVDKEINGNGAGIAIRKGDPLKEKLNTAIAAIRASG
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
EYKKIQDKYFDFDVYGE
CHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]