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The map label for this gene is pleD [H]

Identifier: 209549612

GI number: 209549612

Start: 2081200

End: 2082267

Strand: Direct

Name: pleD [H]

Synonym: Rleg2_2019

Alternate gene names: 209549612

Gene position: 2081200-2082267 (Clockwise)

Preceding gene: 209549611

Following gene: 209549615

Centisome position: 45.86

GC content: 61.52

Gene sequence:

>1068_bases
ATGAATACGGCTGTCGCGCCCAAGGTGCAGGTTCCCGATGTTGCGGGCCAGATCACCTATGCCATGCGCTCGATGGGCGT
TGCGCCGATACCGCGCAATTACGAACTCTTCTACGAGGCATATATCGGCTCCAATCCGGCGCTCACCCGCGAGCTTTCAG
CGCTCGGCAGCCAGGCGACACAGGCCGAACTCGACGCGCTCGGCGCGCAATATTTCACCAGCAGCCCGGCTCGCGTCGTC
GATGACGCCCATACCCGCATATCAGGCGAACTCGACGGCCTGCTGCGGATCCTGAGGCAGGAGCAAAGTTCGCTCGAAAG
CTATACCAGGCTGCTCGGCGAGACCCATAAGCGCATCACTTCCAAGAGCAATGCCAGCGTCGAGCTGATCGAAAACGCCA
TCGAACTTTTGAGCCAGGCGACCGGCGACACCATGGCGCATGGCGAACGCACCGTCGAGGACGTCGTCCAGCGCTCGCAG
GAAATGGACCAAGTCCGCAAGGAACTCGACGAATACAAGCGGATCGCCAACACCGATTCGCTGACGCGCCTGTCCAACCG
CCGCGCCTTCGACGAACGCCTCGCCGCCGTCTTCAACAATCCCGGCATGCGGCCGGTGACGGCCCTGCTGCTCGCCGACA
TCGACAACTTCAAGAAGATCAACGACACCTATGGCCATCCCGTCGGCGACAAGATACTTGCCACCGTCGCCTCCGTCATC
CGCAGCAACGTCCGGCGCGACGTCTTCGTTGCCCGTGCCGGCGGCGAGGAATTCGCCCTCATCATCGACGGCAATACGCC
CGAGGAAATCACGGCAATCGCCGAGCGCATCCGCCGCACGCTGGAAACGACCCCGTTCAAGAATTCGCGCACGCGGGTTA
ATTACGGACCGATCACCGTCTCGATCGGCATCTGCATGGCCTCCAGCGCCGAGGATGCCGGCGAACTCTACAGCAAGACC
GACATCGCCCTTTACGGCGCCAAGAATGCCGGCCGCAACTGCACCATCCTCTATCAGGACGGCATGCAGAAGGATTTCAC
CAAGAGCTGGCTGATCTACAAGACCTGA

Upstream 100 bases:

>100_bases
TGTCGTATGTAGTATTTGGTATATATCGCTTTAATCTTTTCCCAAGAAATTTAAGCTACCAAATAGAATATTGCCTTTGA
ATATATGAGGAGTTCGCCGG

Downstream 100 bases:

>100_bases
CGCCGTTCCGCGCCACGGCGGTTTTACCACATCGTCTTCGCTGCGCGGCCGGGCCATTCCCGGTCGTAGGTTTCCTGGTC
GAAATCGTTGACCGCGGCCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNTAVAPKVQVPDVAGQITYAMRSMGVAPIPRNYELFYEAYIGSNPALTRELSALGSQATQAELDALGAQYFTSSPARVV
DDAHTRISGELDGLLRILRQEQSSLESYTRLLGETHKRITSKSNASVELIENAIELLSQATGDTMAHGERTVEDVVQRSQ
EMDQVRKELDEYKRIANTDSLTRLSNRRAFDERLAAVFNNPGMRPVTALLLADIDNFKKINDTYGHPVGDKILATVASVI
RSNVRRDVFVARAGGEEFALIIDGNTPEEITAIAERIRRTLETTPFKNSRTRVNYGPITVSIGICMASSAEDAGELYSKT
DIALYGAKNAGRNCTILYQDGMQKDFTKSWLIYKT

Sequences:

>Translated_355_residues
MNTAVAPKVQVPDVAGQITYAMRSMGVAPIPRNYELFYEAYIGSNPALTRELSALGSQATQAELDALGAQYFTSSPARVV
DDAHTRISGELDGLLRILRQEQSSLESYTRLLGETHKRITSKSNASVELIENAIELLSQATGDTMAHGERTVEDVVQRSQ
EMDQVRKELDEYKRIANTDSLTRLSNRRAFDERLAAVFNNPGMRPVTALLLADIDNFKKINDTYGHPVGDKILATVASVI
RSNVRRDVFVARAGGEEFALIIDGNTPEEITAIAERIRRTLETTPFKNSRTRVNYGPITVSIGICMASSAEDAGELYSKT
DIALYGAKNAGRNCTILYQDGMQKDFTKSWLIYKT
>Mature_355_residues
MNTAVAPKVQVPDVAGQITYAMRSMGVAPIPRNYELFYEAYIGSNPALTRELSALGSQATQAELDALGAQYFTSSPARVV
DDAHTRISGELDGLLRILRQEQSSLESYTRLLGETHKRITSKSNASVELIENAIELLSQATGDTMAHGERTVEDVVQRSQ
EMDQVRKELDEYKRIANTDSLTRLSNRRAFDERLAAVFNNPGMRPVTALLLADIDNFKKINDTYGHPVGDKILATVASVI
RSNVRRDVFVARAGGEEFALIIDGNTPEEITAIAERIRRTLETTPFKNSRTRVNYGPITVSIGICMASSAEDAGELYSKT
DIALYGAKNAGRNCTILYQDGMQKDFTKSWLIYKT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=188, Percent_Identity=36.7021276595745, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081881, Length=199, Percent_Identity=33.1658291457286, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=32.9787234042553, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=188, Percent_Identity=35.6382978723404, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=35.7575757575758, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87082007, Length=168, Percent_Identity=38.6904761904762, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=35.9756097560976, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=33.695652173913, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=32.5842696629214, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788085, Length=178, Percent_Identity=30.8988764044944, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787056, Length=158, Percent_Identity=34.1772151898734, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=33.9622641509434, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081977, Length=180, Percent_Identity=28.8888888888889, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081974, Length=125, Percent_Identity=28.8, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 39223; Mature: 39223

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAVAPKVQVPDVAGQITYAMRSMGVAPIPRNYELFYEAYIGSNPALTRELSALGSQAT
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHEEEEEHHCCCCHHHHHHHHHCCHHH
QAELDALGAQYFTSSPARVVDDAHTRISGELDGLLRILRQEQSSLESYTRLLGETHKRIT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKSNASVELIENAIELLSQATGDTMAHGERTVEDVVQRSQEMDQVRKELDEYKRIANTDS
CCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTRLSNRRAFDERLAAVFNNPGMRPVTALLLADIDNFKKINDTYGHPVGDKILATVASVI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSNVRRDVFVARAGGEEFALIIDGNTPEEITAIAERIRRTLETTPFKNSRTRVNYGPITV
HHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEE
SIGICMASSAEDAGELYSKTDIALYGAKNAGRNCTILYQDGMQKDFTKSWLIYKT
EEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCEEEEEC
>Mature Secondary Structure
MNTAVAPKVQVPDVAGQITYAMRSMGVAPIPRNYELFYEAYIGSNPALTRELSALGSQAT
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHEEEEEHHCCCCHHHHHHHHHCCHHH
QAELDALGAQYFTSSPARVVDDAHTRISGELDGLLRILRQEQSSLESYTRLLGETHKRIT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKSNASVELIENAIELLSQATGDTMAHGERTVEDVVQRSQEMDQVRKELDEYKRIANTDS
CCCCCHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LTRLSNRRAFDERLAAVFNNPGMRPVTALLLADIDNFKKINDTYGHPVGDKILATVASVI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSNVRRDVFVARAGGEEFALIIDGNTPEEITAIAERIRRTLETTPFKNSRTRVNYGPITV
HHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEE
SIGICMASSAEDAGELYSKTDIALYGAKNAGRNCTILYQDGMQKDFTKSWLIYKT
EEEEEECCCCCHHHHHHCCCCEEEEECCCCCCEEEEEECCCCCHHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA