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The map label for this gene is fumA [H]

Identifier: 209549611

GI number: 209549611

Start: 2079343

End: 2080950

Strand: Direct

Name: fumA [H]

Synonym: Rleg2_2018

Alternate gene names: 209549611

Gene position: 2079343-2080950 (Clockwise)

Preceding gene: 209549609

Following gene: 209549612

Centisome position: 45.82

GC content: 62.25

Gene sequence:

>1608_bases
ATGGCTGACGATCTCTTCCCGCTCGGCAAGGACACCACCCCCTATCGCAAGATCAGCGGCGACTACGTCTCGGTCGACAG
CTTCAAGGGCCAGGAGATCTTGACCGTCGAGCCGGAAGGCATCCGCCTGCTTGCCGAAACCGCCTTTGCCGACATCAACC
ATCTGCTGCGCCCCGGCCACCTGAAGCAGCTCGCCTCGATCCTCGATGATCCGGAGGCGACGGACAATGACCGCTTCGTC
GCCTACGACCTGTTGAAGAATGCCAATATCGCCGCCGGCGGTGTGCTGCCCATGTGCCAGGATACCGGCACGGCGATCAT
CATGGGCAAGAAGGGCCGCCGGGTGTGGACCGAGGGCGGCGATACCGCCGCACTCGCCCGCGGCGTCATGGATGCCTATG
AGAAGAAGAACCTGCGTTATTCGCAGCTCGCGCCGGTGAAAATGTTCGAGGAGAAGAACACCCGCAATAACCTGCCGGCC
CAGATCGACATCTACGAGGAAGGCACCGACGCCTATGAATTCCTCTTCGTCGCCAAGGGCGGCGGCTCGGCCAACAAGAC
CTTTCTCTACCAGGGCACGCCCTCGCTGCTGACGCATGACCGGATGATCGACTTCCTTAAGGAGAAGATCCTCACGTTAG
GGACGGCAGCCTGTCCCCCCTACCATCTGGCGATCGTCATCGGCGGCACCTCGGCCGAGATGAACCTGAAAACTGTCAAG
CTCGCCTCGACCCGCTATCTCGACGAGCTGCCGACCGAAGGGTCCGAAAGCGGCCACGCCTTCCGCGATCTCGAGATGGA
GAAGGAAATCCACAGACTGACACAGCAGATGGGCGTCGGCGCCCAGTTCGGCGGCAAGTATTTCTGTCACGACGTGCGCG
TCATCCGCCTGCCCCGCCACGGCGCTTCGCTGCCGATCGGCCTCGGCGTCTCCTGTTCCGCCGACCGCCAGGCCAAGGGC
AAAATCACCCGCGACGGCATCTTCATCGAACAGCTCGAAAGCGATCCGTCGAAATACATGCCGGAGATCGATGAGGCAAA
ACTGTCGGAATCGACGGTGCATATCGACCTCAACCGGCCGATGGCCGACGTGCTTGCCGAACTGTCGAGACATCCCGTCA
AGACGCGCCTGTCGCTCACCGGCACGATCATCGTCGCCCGCGACCTGGCGCATGCCAAGATCCGCGAGCGGCTGGAAAAC
GGCGAAGGCATGCCCGAGTACCTGAAAAACCACCCGGTCTATTACGCCGGTCCCGCCAAGACGCCGGCCGGTTACGCCTC
CGGCTCCTTCGGTCCGACGACGGCCGGCCGCATGGATAGCTACGTCGACCAGTTCCAGTCCTTCGGCGGCTCCATGGTGA
TGCTCGCCAAGGGCAACCGTTCGCGGGCTGTGCGCGAGGCCTGCAAGAAACACGGCGGCTTCTACCTCGGCTCGATTGGC
GGCCCCGCCGCCCGCCTCGCCCAGGACTGCATCCGCAAGGTCGAAGTCTTCGAATATCCCGAACTCGGCATGGAAGCGGT
GTGGAAGATCGAGGTCGAAAACTTCCCCGCCTTTATCGTCATCGACGACAAGGGCAATGATTTCTTCCAGGAATTGAACC
TGGGGTGA

Upstream 100 bases:

>100_bases
TGGTTCCCCTCCAGAGTCCGGCCGCATCCAGATTGCTTTTGCCCCGCGGCGGATTTAGACCGGTTCCAAATTAGCGACGC
ACACCAGAAAGGTCACTTCG

Downstream 100 bases:

>100_bases
TATCGACAAGTCGAATACGATCTCCTGAAGAGAAGAACTGTAGTTTTTCAGACGCCACTCCTATACAGCGAGTATGCACT
GTCTCGCCAAAGAGCCGTTT

Product: hydro-lyase, Fe-S type, tartrate/fumarate subfamily, subunit beta

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MADDLFPLGKDTTPYRKISGDYVSVDSFKGQEILTVEPEGIRLLAETAFADINHLLRPGHLKQLASILDDPEATDNDRFV
AYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDTAALARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPA
QIDIYEEGTDAYEFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVK
LASTRYLDELPTEGSESGHAFRDLEMEKEIHRLTQQMGVGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQAKG
KITRDGIFIEQLESDPSKYMPEIDEAKLSESTVHIDLNRPMADVLAELSRHPVKTRLSLTGTIIVARDLAHAKIRERLEN
GEGMPEYLKNHPVYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRAVREACKKHGGFYLGSIG
GPAARLAQDCIRKVEVFEYPELGMEAVWKIEVENFPAFIVIDDKGNDFFQELNLG

Sequences:

>Translated_535_residues
MADDLFPLGKDTTPYRKISGDYVSVDSFKGQEILTVEPEGIRLLAETAFADINHLLRPGHLKQLASILDDPEATDNDRFV
AYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDTAALARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPA
QIDIYEEGTDAYEFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVK
LASTRYLDELPTEGSESGHAFRDLEMEKEIHRLTQQMGVGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQAKG
KITRDGIFIEQLESDPSKYMPEIDEAKLSESTVHIDLNRPMADVLAELSRHPVKTRLSLTGTIIVARDLAHAKIRERLEN
GEGMPEYLKNHPVYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRAVREACKKHGGFYLGSIG
GPAARLAQDCIRKVEVFEYPELGMEAVWKIEVENFPAFIVIDDKGNDFFQELNLG
>Mature_534_residues
ADDLFPLGKDTTPYRKISGDYVSVDSFKGQEILTVEPEGIRLLAETAFADINHLLRPGHLKQLASILDDPEATDNDRFVA
YDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGGDTAALARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPAQ
IDIYEEGTDAYEFLFVAKGGGSANKTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVKL
ASTRYLDELPTEGSESGHAFRDLEMEKEIHRLTQQMGVGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCSADRQAKGK
ITRDGIFIEQLESDPSKYMPEIDEAKLSESTVHIDLNRPMADVLAELSRHPVKTRLSLTGTIIVARDLAHAKIRERLENG
EGMPEYLKNHPVYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRAVREACKKHGGFYLGSIGG
PAARLAQDCIRKVEVFEYPELGMEAVWKIEVENFPAFIVIDDKGNDFFQELNLG

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=529, Percent_Identity=66.9187145557656, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=528, Percent_Identity=65.530303030303, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=159, Percent_Identity=30.8176100628931, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 58856; Mature: 58725

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDLFPLGKDTTPYRKISGDYVSVDSFKGQEILTVEPEGIRLLAETAFADINHLLRPGH
CCCCCCCCCCCCCCCEECCCCEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHH
LKQLASILDDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGG
HHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCC
DTAALARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPAQIDIYEEGTDAYEFLFVAKG
CHHHHHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECC
GGSANKTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEE
LASTRYLDELPTEGSESGHAFRDLEMEKEIHRLTQQMGVGAQFGGKYFCHDVRVIRLPRH
EHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEEECCC
GASLPIGLGVSCSADRQAKGKITRDGIFIEQLESDPSKYMPEIDEAKLSESTVHIDLNRP
CCCCCCCCCCCCCCCCCCCCCEECCCEEEEHHCCCHHHHCCCCCHHHCCCCEEEEECCCC
MADVLAELSRHPVKTRLSLTGTIIVARDLAHAKIRERLENGEGMPEYLKNHPVYYAGPAK
HHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCC
TPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRAVREACKKHGGFYLGSIG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEECCC
GPAARLAQDCIRKVEVFEYPELGMEAVWKIEVENFPAFIVIDDKGNDFFQELNLG
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHCCCC
>Mature Secondary Structure 
ADDLFPLGKDTTPYRKISGDYVSVDSFKGQEILTVEPEGIRLLAETAFADINHLLRPGH
CCCCCCCCCCCCCCEECCCCEEEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHCCCHH
LKQLASILDDPEATDNDRFVAYDLLKNANIAAGGVLPMCQDTGTAIIMGKKGRRVWTEGG
HHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCCCEEEEECCCCEEECCCC
DTAALARGVMDAYEKKNLRYSQLAPVKMFEEKNTRNNLPAQIDIYEEGTDAYEFLFVAKG
CHHHHHHHHHHHHHHCCCCHHHCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEEECC
GGSANKTFLYQGTPSLLTHDRMIDFLKEKILTLGTAACPPYHLAIVIGGTSAEMNLKTVK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEEEEE
LASTRYLDELPTEGSESGHAFRDLEMEKEIHRLTQQMGVGAQFGGKYFCHDVRVIRLPRH
EHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECEEEEEECCC
GASLPIGLGVSCSADRQAKGKITRDGIFIEQLESDPSKYMPEIDEAKLSESTVHIDLNRP
CCCCCCCCCCCCCCCCCCCCCEECCCEEEEHHCCCHHHHCCCCCHHHCCCCEEEEECCCC
MADVLAELSRHPVKTRLSLTGTIIVARDLAHAKIRERLENGEGMPEYLKNHPVYYAGPAK
HHHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCCEEEECCCC
TPAGYASGSFGPTTAGRMDSYVDQFQSFGGSMVMLAKGNRSRAVREACKKHGGFYLGSIG
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCEEEECCC
GPAARLAQDCIRKVEVFEYPELGMEAVWKIEVENFPAFIVIDDKGNDFFQELNLG
CHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]