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The map label for this gene is nadE [H]

Identifier: 209549596

GI number: 209549596

Start: 2061527

End: 2063206

Strand: Reverse

Name: nadE [H]

Synonym: Rleg2_2003

Alternate gene names: 209549596

Gene position: 2063206-2061527 (Counterclockwise)

Preceding gene: 209549598

Following gene: 209549595

Centisome position: 45.47

GC content: 62.26

Gene sequence:

>1680_bases
ATGACACAGGAAAACGCTCTCTCTGATATCTTCCGCATCGCTATCGGCCAGCTCAACCCGATGGTCGGCGATGTCGCCGG
CAATCTCGCCAAGGCACGCGAAGCGCGCGCCGATGCGGCGCGCGAAGGCGCGCATCTGCTCGTTCTGACCGAACTCTTCA
TCTCCGGCTATCCGCCTGAAGATCTGGTGCTGAAGCCGGCTTTCATCCGCGCCTGCTGGAAAGCTGTGGAGAGTCTTGCC
GCTGACACCGCCGATGGCGGGCCGGGCGTTCTCATCGGTTTTCCCCGGCAGGACCAGACCGGGCGTTACAATTCCGTCGC
TGTGCTCGACGGTGGCAAGGTGATCGCGGTGCGTGACAAGATCGATCTGCCGAACTACGGCGAATTCGACGAGAAGCGCG
TCTTCAACCAGGGCGCCATGCCGGGGCCGGTGAATTTCCGCGGCGTACGCATCGGCATTCCGATCTGCGAGGATATCTGG
GGCGAGCTCGGCGTCTGCGAAACGCTGGCCGAAAGCGGAGCGGAAATCCTGCTGTCGCCGAACGGTTCGCCCTATTATCG
CGGCAAGGTCGATATCCGCCATCAGGTGGTGCTGAAGCAGGTGATCGAGACCGGTCTGCCGCTGGTCTATGCCGCACAGC
TCGGAGGCCAGGACGAACTCGTGTTCGACGGCGCGAGCTTCGCCTTCAACGCCGACAAGTCGCTGGCCTTCCAGATGAGC
CAGTTCGAAACCGCGCTGGCGGTGACGACATGGAAACGCGGCGGGGCCGGCTGGCACTGCGCCGAAGGGCCGATGGCACA
TATTCCTGAAGGCGAGGAGGCCGACTACCGCGCCTGCCTGCTCGGCTTTCGTGACTACGTCAACAAGAACGGCTTCAAGA
CGGTGGTGCTCGGCCTTTCCGGCGGCATCGACTCGGCGATCTGCACGGCAATCGCCGTCGATGCGCTGGGCGAGGAGCGG
GTGCGCACCGTCATGCTGCCCTATCGCTATACGTCGGAGGATTCGCTGAAGGACGCGGCTGATTGCGCCAAGGCGCTCGG
CTGCCGCTACGACATCGTGCCGATCGAGCAACCGGTGACGGGCTTCAGCAGTGCGCTCTCCGATCTCTTCGCCGGCACGG
AGAGCGGCATCACCGAGGAAAACCTGCAGAGCCGGGCGCGCGGCGTCATCCTGATGGCGATCTCCAACAAATTCGGCGCG
ATGGTGGTGACGACTGGTAATAAGTCGGAAATGTCGGTCGGCTACGCCACGCTTTACGGTGACATGAACGGCGGCTTCAA
TCCGATCAAGGACCTCTACAAGATGCAGGTCTATGCGCTGGCACGCTGGCGCAACGAGAATGTACCGCCGAGTGCGCTGG
GACCGTCGCGCGAGGTGATCCCGAAAAATATCATCGACAAAGCGCCGTCGGCGGAACTGCGTCCCGACCAGAAAGATCAG
GATTCGCTGCCGCCCTATCCTATTCTCGACGATATTCTCGAATGCCTCGTGGAGAAGGAGATGGCGGTCGAAGAAATCGT
CGCGCGCGGCCATGACGTGGTGACCGTCCATCGCATCGAGCACCTGCTCTATCTCGCCGAATATAAGCGACGGCAGTCGG
CGCCGGGCGTGAAGATCACCAAGAAGAATTTCGGCCGCGACCGGCGCTATCCCATCACCAACCGGTTCCGGGATCGCTGA

Upstream 100 bases:

>100_bases
AGGCCGTAGCGCGTCCGTTCACAAAAATTTCGTCATCGCAGGGTGCGGCTTTCGCCGCGGACATGTTGCCGTTGCGGAAG
CTGCAAGCTAAATAAATGAC

Downstream 100 bases:

>100_bases
CGCGACTGAGATTCAAGGGAGGAAACATGCGCAGCTCGGTCGAGATCTACAATGTCAGGACGGGCAGAGCTCGCGAGGTT
TGGCAGACGGATAAGCTGGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MTQENALSDIFRIAIGQLNPMVGDVAGNLAKAREARADAAREGAHLLVLTELFISGYPPEDLVLKPAFIRACWKAVESLA
ADTADGGPGVLIGFPRQDQTGRYNSVAVLDGGKVIAVRDKIDLPNYGEFDEKRVFNQGAMPGPVNFRGVRIGIPICEDIW
GELGVCETLAESGAEILLSPNGSPYYRGKVDIRHQVVLKQVIETGLPLVYAAQLGGQDELVFDGASFAFNADKSLAFQMS
QFETALAVTTWKRGGAGWHCAEGPMAHIPEGEEADYRACLLGFRDYVNKNGFKTVVLGLSGGIDSAICTAIAVDALGEER
VRTVMLPYRYTSEDSLKDAADCAKALGCRYDIVPIEQPVTGFSSALSDLFAGTESGITEENLQSRARGVILMAISNKFGA
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNENVPPSALGPSREVIPKNIIDKAPSAELRPDQKDQ
DSLPPYPILDDILECLVEKEMAVEEIVARGHDVVTVHRIEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR

Sequences:

>Translated_559_residues
MTQENALSDIFRIAIGQLNPMVGDVAGNLAKAREARADAAREGAHLLVLTELFISGYPPEDLVLKPAFIRACWKAVESLA
ADTADGGPGVLIGFPRQDQTGRYNSVAVLDGGKVIAVRDKIDLPNYGEFDEKRVFNQGAMPGPVNFRGVRIGIPICEDIW
GELGVCETLAESGAEILLSPNGSPYYRGKVDIRHQVVLKQVIETGLPLVYAAQLGGQDELVFDGASFAFNADKSLAFQMS
QFETALAVTTWKRGGAGWHCAEGPMAHIPEGEEADYRACLLGFRDYVNKNGFKTVVLGLSGGIDSAICTAIAVDALGEER
VRTVMLPYRYTSEDSLKDAADCAKALGCRYDIVPIEQPVTGFSSALSDLFAGTESGITEENLQSRARGVILMAISNKFGA
MVVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNENVPPSALGPSREVIPKNIIDKAPSAELRPDQKDQ
DSLPPYPILDDILECLVEKEMAVEEIVARGHDVVTVHRIEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR
>Mature_558_residues
TQENALSDIFRIAIGQLNPMVGDVAGNLAKAREARADAAREGAHLLVLTELFISGYPPEDLVLKPAFIRACWKAVESLAA
DTADGGPGVLIGFPRQDQTGRYNSVAVLDGGKVIAVRDKIDLPNYGEFDEKRVFNQGAMPGPVNFRGVRIGIPICEDIWG
ELGVCETLAESGAEILLSPNGSPYYRGKVDIRHQVVLKQVIETGLPLVYAAQLGGQDELVFDGASFAFNADKSLAFQMSQ
FETALAVTTWKRGGAGWHCAEGPMAHIPEGEEADYRACLLGFRDYVNKNGFKTVVLGLSGGIDSAICTAIAVDALGEERV
RTVMLPYRYTSEDSLKDAADCAKALGCRYDIVPIEQPVTGFSSALSDLFAGTESGITEENLQSRARGVILMAISNKFGAM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALARWRNENVPPSALGPSREVIPKNIIDKAPSAELRPDQKDQD
SLPPYPILDDILECLVEKEMAVEEIVARGHDVVTVHRIEHLLYLAEYKRRQSAPGVKITKKNFGRDRRYPITNRFRDR

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=261, Percent_Identity=28.735632183908, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321865, Length=606, Percent_Identity=22.7722772277228, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61084; Mature: 60953

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQENALSDIFRIAIGQLNPMVGDVAGNLAKAREARADAAREGAHLLVLTELFISGYPPE
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCH
DLVLKPAFIRACWKAVESLAADTADGGPGVLIGFPRQDQTGRYNSVAVLDGGKVIAVRDK
HCEECHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEECC
IDLPNYGEFDEKRVFNQGAMPGPVNFRGVRIGIPICEDIWGELGVCETLAESGAEILLSP
CCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECC
NGSPYYRGKVDIRHQVVLKQVIETGLPLVYAAQLGGQDELVFDGASFAFNADKSLAFQMS
CCCCEEECEECHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCHHHHHHH
QFETALAVTTWKRGGAGWHCAEGPMAHIPEGEEADYRACLLGFRDYVNKNGFKTVVLGLS
HHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GGIDSAICTAIAVDALGEERVRTVMLPYRYTSEDSLKDAADCAKALGCRYDIVPIEQPVT
CCCHHHHHHHHHHHHCCCCCCEEEEECEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCH
GFSSALSDLFAGTESGITEENLQSRARGVILMAISNKFGAMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYALARWRNENVPPSALGPSREVIPKNIIDKAPSAELRPDQKDQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCC
DSLPPYPILDDILECLVEKEMAVEEIVARGHDVVTVHRIEHLLYLAEYKRRQSAPGVKIT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEE
KKNFGRDRRYPITNRFRDR
HHHCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
TQENALSDIFRIAIGQLNPMVGDVAGNLAKAREARADAAREGAHLLVLTELFISGYPPE
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCH
DLVLKPAFIRACWKAVESLAADTADGGPGVLIGFPRQDQTGRYNSVAVLDGGKVIAVRDK
HCEECHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEECC
IDLPNYGEFDEKRVFNQGAMPGPVNFRGVRIGIPICEDIWGELGVCETLAESGAEILLSP
CCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCHHHHHHHHHCCCEEEECC
NGSPYYRGKVDIRHQVVLKQVIETGLPLVYAAQLGGQDELVFDGASFAFNADKSLAFQMS
CCCCEEECEECHHHHHHHHHHHHCCCCEEEEECCCCCCCEEECCCCEEECCCCHHHHHHH
QFETALAVTTWKRGGAGWHCAEGPMAHIPEGEEADYRACLLGFRDYVNKNGFKTVVLGLS
HHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEC
GGIDSAICTAIAVDALGEERVRTVMLPYRYTSEDSLKDAADCAKALGCRYDIVPIEQPVT
CCCHHHHHHHHHHHHCCCCCCEEEEECEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCH
GFSSALSDLFAGTESGITEENLQSRARGVILMAISNKFGAMVVTTGNKSEMSVGYATLYG
HHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEEE
DMNGGFNPIKDLYKMQVYALARWRNENVPPSALGPSREVIPKNIIDKAPSAELRPDQKDQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHHCCCCCCCCCCCCCCC
DSLPPYPILDDILECLVEKEMAVEEIVARGHDVVTVHRIEHLLYLAEYKRRQSAPGVKIT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEE
KKNFGRDRRYPITNRFRDR
HHHCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]