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The map label for this gene is tolB [C]

Identifier: 209549595

GI number: 209549595

Start: 2060670

End: 2061500

Strand: Reverse

Name: tolB [C]

Synonym: Rleg2_2002

Alternate gene names: 209549595

Gene position: 2061500-2060670 (Counterclockwise)

Preceding gene: 209549596

Following gene: 209549594

Centisome position: 45.43

GC content: 61.49

Gene sequence:

>831_bases
ATGCGCAGCTCGGTCGAGATCTACAATGTCAGGACGGGCAGAGCTCGCGAGGTTTGGCAGACGGATAAGCTGGTCGAGGC
GCCGAACTTCTCGCCCGACGGTTGCTATCTGCTCTTGAACGGCGACGGGCTGCTCTGGCGGCTGCCGCTCGATGGCGGCG
AAGTCGTCAAGGTCGATACCGGCTTTGCCGTCAACTGCAACAATGATCACGGCATTTCACCCGATGGCACCGAGATCGTC
ATCTCTGATAAGACCGAATTCGGCAAGTCGACGATCTATGTCCTGCCGATCGAGGGCGGCACGCCGCGGCTGGTCACCGA
GAACCAGCCGTCCTACTGGCACGGCTGGTCGCCCGACGGCTGCCAGCTTGCCTATTGCGGCATCCGCAACGATCTCTTCG
ATATCTATACGATCTCCGTCGACGGCGGCGCCGAGACGCGGCTGACGCATGGCGAGGGCCGCAACGACGGGCCGGACTAT
TCGGCCGACGGACAATGGATCTATTTCAATTCCAGCCGCACTGGGCTGATGCAGATCTGGCGCATCCACCCCGATGGCAG
CGGGCTGGAGCAGGTGACGTCGGACAATCAGGGCAACTGGTTCGCCCATCCGTCGCCCAGCAATGACAAGGTGCTGATCC
TATCCTACGACCCCTCGGTTTTCGACCACCCGCGAGACCTCGACGTGCGGCTGCGGCTGATGGACATGGATGGCGGCAAC
CTGAAGACGCTGTTCGAGCTGTTCGGCGGGCAGGGCACGATCAACGTGCCCTGCTGGTCACCAGATGGCGAGGAATTCGC
CTATGTCCGTTATTTCCCGGTGAGATCTTGA

Upstream 100 bases:

>100_bases
GCGTGAAGATCACCAAGAAGAATTTCGGCCGCGACCGGCGCTATCCCATCACCAACCGGTTCCGGGATCGCTGACGCGAC
TGAGATTCAAGGGAGGAAAC

Downstream 100 bases:

>100_bases
GACGGGCGCGCCGCCATTTGATGATGGGGCGGTGGGTATCTGATGACATCGAGAGGCCTACTCGAACACTTTGCTAGAAA
CCGTTTCAGAAACGGGCTGT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRSSVEIYNVRTGRAREVWQTDKLVEAPNFSPDGCYLLLNGDGLLWRLPLDGGEVVKVDTGFAVNCNNDHGISPDGTEIV
ISDKTEFGKSTIYVLPIEGGTPRLVTENQPSYWHGWSPDGCQLAYCGIRNDLFDIYTISVDGGAETRLTHGEGRNDGPDY
SADGQWIYFNSSRTGLMQIWRIHPDGSGLEQVTSDNQGNWFAHPSPSNDKVLILSYDPSVFDHPRDLDVRLRLMDMDGGN
LKTLFELFGGQGTINVPCWSPDGEEFAYVRYFPVRS

Sequences:

>Translated_276_residues
MRSSVEIYNVRTGRAREVWQTDKLVEAPNFSPDGCYLLLNGDGLLWRLPLDGGEVVKVDTGFAVNCNNDHGISPDGTEIV
ISDKTEFGKSTIYVLPIEGGTPRLVTENQPSYWHGWSPDGCQLAYCGIRNDLFDIYTISVDGGAETRLTHGEGRNDGPDY
SADGQWIYFNSSRTGLMQIWRIHPDGSGLEQVTSDNQGNWFAHPSPSNDKVLILSYDPSVFDHPRDLDVRLRLMDMDGGN
LKTLFELFGGQGTINVPCWSPDGEEFAYVRYFPVRS
>Mature_276_residues
MRSSVEIYNVRTGRAREVWQTDKLVEAPNFSPDGCYLLLNGDGLLWRLPLDGGEVVKVDTGFAVNCNNDHGISPDGTEIV
ISDKTEFGKSTIYVLPIEGGTPRLVTENQPSYWHGWSPDGCQLAYCGIRNDLFDIYTISVDGGAETRLTHGEGRNDGPDY
SADGQWIYFNSSRTGLMQIWRIHPDGSGLEQVTSDNQGNWFAHPSPSNDKVLILSYDPSVFDHPRDLDVRLRLMDMDGGN
LKTLFELFGGQGTINVPCWSPDGEEFAYVRYFPVRS

Specific function: Involved In The Tonb-Independent Uptake Of Group A Colicins (Colicins A, E1, E2, E3 And K). Necessary For The Colicins To Reach Their Respective Targets After Initial Binding To The Bacteria. [C]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011042
- InterPro:   IPR011659 [H]

Pfam domain/function: PF07676 PD40 [H]

EC number: NA

Molecular weight: Translated: 30791; Mature: 30791

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSVEIYNVRTGRAREVWQTDKLVEAPNFSPDGCYLLLNGDGLLWRLPLDGGEVVKVDT
CCCCEEEEEECCCCCHHHHCCCCEEECCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEC
GFAVNCNNDHGISPDGTEIVISDKTEFGKSTIYVLPIEGGTPRLVTENQPSYWHGWSPDG
CEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEEECCCCCEECCCCCCC
CQLAYCGIRNDLFDIYTISVDGGAETRLTHGEGRNDGPDYSADGQWIYFNSSRTGLMQIW
CEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEEEE
RIHPDGSGLEQVTSDNQGNWFAHPSPSNDKVLILSYDPSVFDHPRDLDVRLRLMDMDGGN
EECCCCCCHHHHHCCCCCCEEECCCCCCCEEEEEEECCCHHCCCCCCEEEEEEEECCCCC
LKTLFELFGGQGTINVPCWSPDGEEFAYVRYFPVRS
HHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MRSSVEIYNVRTGRAREVWQTDKLVEAPNFSPDGCYLLLNGDGLLWRLPLDGGEVVKVDT
CCCCEEEEEECCCCCHHHHCCCCEEECCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEC
GFAVNCNNDHGISPDGTEIVISDKTEFGKSTIYVLPIEGGTPRLVTENQPSYWHGWSPDG
CEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCEEEECCCCCEECCCCCCC
CQLAYCGIRNDLFDIYTISVDGGAETRLTHGEGRNDGPDYSADGQWIYFNSSRTGLMQIW
CEEEEECCCCCEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCEEEEE
RIHPDGSGLEQVTSDNQGNWFAHPSPSNDKVLILSYDPSVFDHPRDLDVRLRLMDMDGGN
EECCCCCCHHHHHCCCCCCEEECCCCCCCEEEEEEECCCHHCCCCCCEEEEEEEECCCCC
LKTLFELFGGQGTINVPCWSPDGEEFAYVRYFPVRS
HHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]