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The map label for this gene is ycjG [H]

Identifier: 209549579

GI number: 209549579

Start: 2037868

End: 2038851

Strand: Direct

Name: ycjG [H]

Synonym: Rleg2_1985

Alternate gene names: 209549579

Gene position: 2037868-2038851 (Clockwise)

Preceding gene: 209549573

Following gene: 209549582

Centisome position: 44.91

GC content: 65.96

Gene sequence:

>984_bases
ATGCCGCGCACCCTCAATATCCAGATGAATTCCTTTCCGATTGCCGGAGCCTTTACCATCTCGCGCGGCGCCAAGACCGA
GGCCGAGGTGATTACCTGCACGCTTGCCGAAGACGGTGTGCGGGGGCTTGGCGAATGCGTGCCCTATCGCCGCTACGGCG
AGACCATGGAGAGCGTGTTCGCCCAGATCGAAGCCGCGCGTCCGCTGGTCGAGGCCGGGATCTCGCGCCGCGACCTGTTG
TCGGCGATGCCGCCGGGCGCTGCCCGCAATGCCGTCGATTGCGCCCTTTGGGACCTCGAGGCCAAACGAACGGGTGAAAC
CGTTGCGGCCCGCCTCGGCCTTGCCGATTTGAAGCCGCTCACCACCGCCTATACCATTTCGCTCGGTGAACCGGAGGTGA
TGGCCGCAAAGGCGCGCGAACATGCCGGTCGCGCGCTGCTCAAGGTCAAGGTCGGCACCGGCAACGATGAAAGCCGCATC
CGCGCCGTCTGCGCCGCCGCGCCCGATGCAACAATCATCCTCGATGCCAATGAGGGCTGGCCGGAAGCGGTGCTGGAACG
TCATCTCGATATCGCGGCGCAAGCCGGTGTGGCTCTCGTCGAGCAGCCGCTGCCGGCCGGCCGCGACGCACTGCTTGCCG
AAATCCGCCGCCCGCTCCTCGTCTGCGCCGACGAAAGTGTCCACCATACCGGCGATCTTGCAAGCATTGCCGACCGCTAT
GACGCGATCAACATCAAGCTCGACAAGACCGGCGGCCTGACGGAAGCCCTGGCGATGAAGGCGGAAGCCGAGCGGCTTGG
TTTCAGCATCATGATCGGCTGCATGGTCGGCACCTCGCTTGCCATGGCGCCGGCGGTGCTGCTCGCCCAGAATGCCGATT
TCGTCGATCTCGACGGCCCGCTGCTGCTTGCCCGCGACCGCGAACCCGGCTTGCGCTACGCCGCCTCCCTGGTCTTTCCG
CCCGAAAGCACGCTCTGGGGCTGA

Upstream 100 bases:

>100_bases
AGGCTTAAGGGCGACATCGCCGACTCGGCGGATCCTTCGCCGCCCGGCGCGTCAATATACAGAAGCAACGTGCGATTTCC
ATGCTCACAGGACAGCAATA

Downstream 100 bases:

>100_bases
AGGTAGTAGGCCGAGATGACCAGACCGAGGCCGGAGAATGCGACCAGCGCCATGACGTAATAGCTGACGACGCCGAGCCA
GGCATAGAGATAACCCGACG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MPRTLNIQMNSFPIAGAFTISRGAKTEAEVITCTLAEDGVRGLGECVPYRRYGETMESVFAQIEAARPLVEAGISRRDLL
SAMPPGAARNAVDCALWDLEAKRTGETVAARLGLADLKPLTTAYTISLGEPEVMAAKAREHAGRALLKVKVGTGNDESRI
RAVCAAAPDATIILDANEGWPEAVLERHLDIAAQAGVALVEQPLPAGRDALLAEIRRPLLVCADESVHHTGDLASIADRY
DAINIKLDKTGGLTEALAMKAEAERLGFSIMIGCMVGTSLAMAPAVLLAQNADFVDLDGPLLLARDREPGLRYAASLVFP
PESTLWG

Sequences:

>Translated_327_residues
MPRTLNIQMNSFPIAGAFTISRGAKTEAEVITCTLAEDGVRGLGECVPYRRYGETMESVFAQIEAARPLVEAGISRRDLL
SAMPPGAARNAVDCALWDLEAKRTGETVAARLGLADLKPLTTAYTISLGEPEVMAAKAREHAGRALLKVKVGTGNDESRI
RAVCAAAPDATIILDANEGWPEAVLERHLDIAAQAGVALVEQPLPAGRDALLAEIRRPLLVCADESVHHTGDLASIADRY
DAINIKLDKTGGLTEALAMKAEAERLGFSIMIGCMVGTSLAMAPAVLLAQNADFVDLDGPLLLARDREPGLRYAASLVFP
PESTLWG
>Mature_326_residues
PRTLNIQMNSFPIAGAFTISRGAKTEAEVITCTLAEDGVRGLGECVPYRRYGETMESVFAQIEAARPLVEAGISRRDLLS
AMPPGAARNAVDCALWDLEAKRTGETVAARLGLADLKPLTTAYTISLGEPEVMAAKAREHAGRALLKVKVGTGNDESRIR
AVCAAAPDATIILDANEGWPEAVLERHLDIAAQAGVALVEQPLPAGRDALLAEIRRPLLVCADESVHHTGDLASIADRYD
AINIKLDKTGGLTEALAMKAEAERLGFSIMIGCMVGTSLAMAPAVLLAQNADFVDLDGPLLLARDREPGLRYAASLVFPP
ESTLWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=313, Percent_Identity=46.3258785942492, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34721; Mature: 34590

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTLNIQMNSFPIAGAFTISRGAKTEAEVITCTLAEDGVRGLGECVPYRRYGETMESVF
CCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHH
AQIEAARPLVEAGISRRDLLSAMPPGAARNAVDCALWDLEAKRTGETVAARLGLADLKPL
HHHHHCCHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCHHHCCHHHHHHHCCHHCCCC
TTAYTISLGEPEVMAAKAREHAGRALLKVKVGTGNDESRIRAVCAAAPDATIILDANEGW
CEEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHEEEECCCCEEEEEECCCCC
PEAVLERHLDIAAQAGVALVEQPLPAGRDALLAEIRRPLLVCADESVHHTGDLASIADRY
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCC
DAINIKLDKTGGLTEALAMKAEAERLGFSIMIGCMVGTSLAMAPAVLLAQNADFVDLDGP
CEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHEECCCCEEECCCC
LLLARDREPGLRYAASLVFPPESTLWG
EEEEECCCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
PRTLNIQMNSFPIAGAFTISRGAKTEAEVITCTLAEDGVRGLGECVPYRRYGETMESVF
CCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHH
AQIEAARPLVEAGISRRDLLSAMPPGAARNAVDCALWDLEAKRTGETVAARLGLADLKPL
HHHHHCCHHHHHCCCHHHHHHHCCCCCCCCCCEEEEEECCHHHCCHHHHHHHCCHHCCCC
TTAYTISLGEPEVMAAKAREHAGRALLKVKVGTGNDESRIRAVCAAAPDATIILDANEGW
CEEEEEECCCCHHHHHHHHHHCCEEEEEEEECCCCCHHHHHEEEECCCCEEEEEECCCCC
PEAVLERHLDIAAQAGVALVEQPLPAGRDALLAEIRRPLLVCADESVHHTGDLASIADRY
HHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHCC
DAINIKLDKTGGLTEALAMKAEAERLGFSIMIGCMVGTSLAMAPAVLLAQNADFVDLDGP
CEEEEEEECCCCHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHHHHHHEECCCCEEECCCC
LLLARDREPGLRYAASLVFPPESTLWG
EEEEECCCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]