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The map label for this gene is pleD [H]

Identifier: 209549574

GI number: 209549574

Start: 2032332

End: 2033585

Strand: Reverse

Name: pleD [H]

Synonym: Rleg2_1980

Alternate gene names: 209549574

Gene position: 2033585-2032332 (Counterclockwise)

Preceding gene: 209549575

Following gene: 209549572

Centisome position: 44.81

GC content: 60.45

Gene sequence:

>1254_bases
ATGGGGGTCGCACGAGTATTCGGAAAGTCGTCGTCTGAGGCGATCCAGCGCGATAACGACCGACTGGCTGCGCTGCAGCA
GCTTGATCTTCTCGACACGCCGCGGGACGAAGGATTTGAGCGGATCGTCCGCCTGATAAAGCAGATCTTTTCCATCGACA
TAGGCATCGTCTCCCTGATCGATGCGCACCGGCAATGGTACAAGGCCTGCTCCGGCCTCGCCGCCGACGAAGTGTCGCTC
GACGACACGTTCTGCCGCTGCGTGGTGGACTGCGACGAGCCCGTTATCGTGCAGGATGCGACCAAGGACGCGCGCTTTTC
AAAACATTCTGCGGTGACCGGCGAGGACCATATCCGCTTTTATGCCGGCGTTCCGTTGCGAACGAAGGCCGGGCATATGA
TCGGAACGGTCTGCGCGATCGACCGCCGGCCGAGATCGTTCGGCAGCAGAGATCTCGATATTCTTCAGGAGCTGGCGGGG
GCGGCGATGGACCGCATCGAACTCGTGCAGTCGGCCGCCACCGACAGCCTGACCGAGGCGATGACGCGGCGCGCTTTCAA
GCAGGAAGCCGATCAGCTGATTTCGCTCGCTTTGCGGCATCAGCATGATCTGTCCTGCATCGCCTTCGATATCGACCATT
TCAAGAAGGTGAACGATACCTATGGGCATGCCGCGGGAGATGCGGTTCTCAAGGCGGTTGCCTCGATTTGCAAGGCGACC
CTTCGCGCCGGCGATCTGTTCGGGCGTATCGGAGGCGAGGAATTTGCCGTCATCCTGCCGCATGTCGACCGGGAAGGGGC
TTTCGCCGTCGCCGAAAAGCTCAGAGCCGCCATCGCCGCGCAGCCCATTCGCGGCGATCACGGCTCCTTGAATGTCACGG
CGAGCTTCGGAACGTCGGCGCTCTCGATCGTCAGCAAGGAGATCGAGACGCTGTTGGCTCAGGCGGATGCCGCGATGTAC
CAGGCCAAACATGCGGGACGAAATCGTTGTGTGTCATGGAGTTCCATCCACGCCGATCACGCTATCGGCGCGCGGCGGCG
AGTGCTGAAGGCGGGATCGATCCTGTTCAACGATCGACGCTCGACCATCGACTGCACCGTCAAATCCATCGGCTCGGAAA
GCGCCGGTATTTCGGTTTCCAGTTCGGCCGGCATTCCTTCCGAGTTCATTCTTGCAATTAAGGGAGAAGGATTCGAAACG
AATTGCCGGGTGATCGCTCAGGACCGGCAGCACCTGGAAGTGGCCTTCAGATAG

Upstream 100 bases:

>100_bases
AAAAATATGCGGCCCCATGGCGGCAGGGCTGCCATCGTATTCAATTTCTTCATGAATTTGTGGAACGCATCATGTTATCG
CTGTCAGGAGGCATGCTTGC

Downstream 100 bases:

>100_bases
CTGGAGCAATGCCAGGAAAAGTGCAAAGCGGTTTTCCGTCCGGAATTGCGTAGAAACAAAAACGAGGGCGGTTCTGCGCT
TCTGTCAAAAGCTGAACCGT

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGVARVFGKSSSEAIQRDNDRLAALQQLDLLDTPRDEGFERIVRLIKQIFSIDIGIVSLIDAHRQWYKACSGLAADEVSL
DDTFCRCVVDCDEPVIVQDATKDARFSKHSAVTGEDHIRFYAGVPLRTKAGHMIGTVCAIDRRPRSFGSRDLDILQELAG
AAMDRIELVQSAATDSLTEAMTRRAFKQEADQLISLALRHQHDLSCIAFDIDHFKKVNDTYGHAAGDAVLKAVASICKAT
LRAGDLFGRIGGEEFAVILPHVDREGAFAVAEKLRAAIAAQPIRGDHGSLNVTASFGTSALSIVSKEIETLLAQADAAMY
QAKHAGRNRCVSWSSIHADHAIGARRRVLKAGSILFNDRRSTIDCTVKSIGSESAGISVSSSAGIPSEFILAIKGEGFET
NCRVIAQDRQHLEVAFR

Sequences:

>Translated_417_residues
MGVARVFGKSSSEAIQRDNDRLAALQQLDLLDTPRDEGFERIVRLIKQIFSIDIGIVSLIDAHRQWYKACSGLAADEVSL
DDTFCRCVVDCDEPVIVQDATKDARFSKHSAVTGEDHIRFYAGVPLRTKAGHMIGTVCAIDRRPRSFGSRDLDILQELAG
AAMDRIELVQSAATDSLTEAMTRRAFKQEADQLISLALRHQHDLSCIAFDIDHFKKVNDTYGHAAGDAVLKAVASICKAT
LRAGDLFGRIGGEEFAVILPHVDREGAFAVAEKLRAAIAAQPIRGDHGSLNVTASFGTSALSIVSKEIETLLAQADAAMY
QAKHAGRNRCVSWSSIHADHAIGARRRVLKAGSILFNDRRSTIDCTVKSIGSESAGISVSSSAGIPSEFILAIKGEGFET
NCRVIAQDRQHLEVAFR
>Mature_416_residues
GVARVFGKSSSEAIQRDNDRLAALQQLDLLDTPRDEGFERIVRLIKQIFSIDIGIVSLIDAHRQWYKACSGLAADEVSLD
DTFCRCVVDCDEPVIVQDATKDARFSKHSAVTGEDHIRFYAGVPLRTKAGHMIGTVCAIDRRPRSFGSRDLDILQELAGA
AMDRIELVQSAATDSLTEAMTRRAFKQEADQLISLALRHQHDLSCIAFDIDHFKKVNDTYGHAAGDAVLKAVASICKATL
RAGDLFGRIGGEEFAVILPHVDREGAFAVAEKLRAAIAAQPIRGDHGSLNVTASFGTSALSIVSKEIETLLAQADAAMYQ
AKHAGRNRCVSWSSIHADHAIGARRRVLKAGSILFNDRRSTIDCTVKSIGSESAGISVSSSAGIPSEFILAIKGEGFETN
CRVIAQDRQHLEVAFR

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=40, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=36.6459627329193, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081977, Length=283, Percent_Identity=27.9151943462898, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=36.9047619047619, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788381, Length=210, Percent_Identity=30, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786584, Length=157, Percent_Identity=34.3949044585987, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=30.8641975308642, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=33.1210191082803, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788085, Length=183, Percent_Identity=27.3224043715847, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=29.8245614035088, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=173, Percent_Identity=32.3699421965318, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45288; Mature: 45157

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVARVFGKSSSEAIQRDNDRLAALQQLDLLDTPRDEGFERIVRLIKQIFSIDIGIVSLI
CCCHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAHRQWYKACSGLAADEVSLDDTFCRCVVDCDEPVIVQDATKDARFSKHSAVTGEDHIRF
HHHHHHHHHHCCCCCCCCCCCHHHHHHEECCCCCEEEECCCCCHHCCCCCCCCCCCCEEE
YAGVPLRTKAGHMIGTVCAIDRRPRSFGSRDLDILQELAGAAMDRIELVQSAATDSLTEA
EECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTRRAFKQEADQLISLALRHQHDLSCIAFDIDHFKKVNDTYGHAAGDAVLKAVASICKAT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LRAGDLFGRIGGEEFAVILPHVDREGAFAVAEKLRAAIAAQPIRGDHGSLNVTASFGTSA
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHH
LSIVSKEIETLLAQADAAMYQAKHAGRNRCVSWSSIHADHAIGARRRVLKAGSILFNDRR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCEEECCCC
STIDCTVKSIGSESAGISVSSSAGIPSEFILAIKGEGFETNCRVIAQDRQHLEVAFR
CEEEHHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCHHEEEECC
>Mature Secondary Structure 
GVARVFGKSSSEAIQRDNDRLAALQQLDLLDTPRDEGFERIVRLIKQIFSIDIGIVSLI
CCHHHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DAHRQWYKACSGLAADEVSLDDTFCRCVVDCDEPVIVQDATKDARFSKHSAVTGEDHIRF
HHHHHHHHHHCCCCCCCCCCCHHHHHHEECCCCCEEEECCCCCHHCCCCCCCCCCCCEEE
YAGVPLRTKAGHMIGTVCAIDRRPRSFGSRDLDILQELAGAAMDRIELVQSAATDSLTEA
EECCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTRRAFKQEADQLISLALRHQHDLSCIAFDIDHFKKVNDTYGHAAGDAVLKAVASICKAT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
LRAGDLFGRIGGEEFAVILPHVDREGAFAVAEKLRAAIAAQPIRGDHGSLNVTASFGTSA
HHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHH
LSIVSKEIETLLAQADAAMYQAKHAGRNRCVSWSSIHADHAIGARRRVLKAGSILFNDRR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCEEECCCC
STIDCTVKSIGSESAGISVSSSAGIPSEFILAIKGEGFETNCRVIAQDRQHLEVAFR
CEEEHHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA