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The map label for this gene is dacA [H]

Identifier: 209549573

GI number: 209549573

Start: 2031342

End: 2032217

Strand: Direct

Name: dacA [H]

Synonym: Rleg2_1979

Alternate gene names: 209549573

Gene position: 2031342-2032217 (Clockwise)

Preceding gene: 209549570

Following gene: 209549579

Centisome position: 44.76

GC content: 63.01

Gene sequence:

>876_bases
ATGACCAAGACGACCTCCCGGCTCCTTGCCACCGCGCTCTCTTCCGCCGTTCTGCTGGGCGCGTTCACCCTGGCCGAGGC
CGGCCAGGCTAGCTTTGTCGTCGATGCGCAATCCGGTCAGGTTCTCGAAGCCTCCAATCAGGACGATCTGAACTATCCGG
CGTCGCTGACCAAGATGATGACGCTCTACCTCGCCTTCGAGGCCCTGCATGAGGGACGCCTCACCTGGGACCAGAAGCTC
ACCATGTCGGAAAATGCCGAGAGCAAGGAGCCGTTCAAGCTCGCCGTCGGCGCCGGCCGCCAGGTGACGCTGCGCGAAGC
GGTCGAAAGCATCGTCGTCTTGTCCGCCAACGACTCGGCCGTGGCGATCGCCGAACAGCTCGGCGGCTCCGAACAGGCTT
TTGCCAAAGCGATGACCGACAAGGCGCGCCAGCTCGGCATGACGAATACGGTCTTCAGGAACCCCTCAGGCCTTCCCGAT
CCCGAGCAGGTCACCTCGGCACGAGATATGGCGACCCTCGGCATTTCGCTGATGCGGGACTTTCCCGAGGAGTTCAAGCT
CTTCTCCATGCGCGGCTTCCAGTTCCGCGGCATGAAGCTGCGCGGTCACAACAACCTCATGTATCGTTATGACGGCGTCG
ACGGCATCAAGACCGGCTATACCGATGCATCGGGCTATAATGTCGTGACATCGGCGCTGAAGGACGGTCGCCGCGTTGTC
GGCGTCGTCATGGGCGAAAAGACAGCGAGCCTGCGCGACGACAAGATGGCCGGCCTGCTGGACAGCACGCTGCCGTCGTC
CTCGACCGCCACGGCCTCCACGATGCCAGGCAAACAGCCCGGAGCGACGATGACGGATGCGCAGCCGACGAAATAG

Upstream 100 bases:

>100_bases
TTATTGCCGCCTGTCCTTCATTTCGTCACAAACTTTCTCGATCCGCTTGCGTTTAGCCAACGGCTGACCCGAGCTTCCAA
CGACAGAGACAGTAACATCG

Downstream 100 bases:

>100_bases
GCTGCTGTCCGAGAACGGTTCAGCTTTTGACAGAAGCGCAGAACCGCCCTCGTTTTTGTTTCTACGCAATTCCGGACGGA
AAACCGCTTTGCACTTTTCC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTKTTSRLLATALSSAVLLGAFTLAEAGQASFVVDAQSGQVLEASNQDDLNYPASLTKMMTLYLAFEALHEGRLTWDQKL
TMSENAESKEPFKLAVGAGRQVTLREAVESIVVLSANDSAVAIAEQLGGSEQAFAKAMTDKARQLGMTNTVFRNPSGLPD
PEQVTSARDMATLGISLMRDFPEEFKLFSMRGFQFRGMKLRGHNNLMYRYDGVDGIKTGYTDASGYNVVTSALKDGRRVV
GVVMGEKTASLRDDKMAGLLDSTLPSSSTATASTMPGKQPGATMTDAQPTK

Sequences:

>Translated_291_residues
MTKTTSRLLATALSSAVLLGAFTLAEAGQASFVVDAQSGQVLEASNQDDLNYPASLTKMMTLYLAFEALHEGRLTWDQKL
TMSENAESKEPFKLAVGAGRQVTLREAVESIVVLSANDSAVAIAEQLGGSEQAFAKAMTDKARQLGMTNTVFRNPSGLPD
PEQVTSARDMATLGISLMRDFPEEFKLFSMRGFQFRGMKLRGHNNLMYRYDGVDGIKTGYTDASGYNVVTSALKDGRRVV
GVVMGEKTASLRDDKMAGLLDSTLPSSSTATASTMPGKQPGATMTDAQPTK
>Mature_290_residues
TKTTSRLLATALSSAVLLGAFTLAEAGQASFVVDAQSGQVLEASNQDDLNYPASLTKMMTLYLAFEALHEGRLTWDQKLT
MSENAESKEPFKLAVGAGRQVTLREAVESIVVLSANDSAVAIAEQLGGSEQAFAKAMTDKARQLGMTNTVFRNPSGLPDP
EQVTSARDMATLGISLMRDFPEEFKLFSMRGFQFRGMKLRGHNNLMYRYDGVDGIKTGYTDASGYNVVTSALKDGRRVVG
VVMGEKTASLRDDKMAGLLDSTLPSSSTATASTMPGKQPGATMTDAQPTK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=239, Percent_Identity=34.3096234309623, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1786851, Length=239, Percent_Identity=35.9832635983264, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI87082022, Length=236, Percent_Identity=32.6271186440678, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI87082054, Length=231, Percent_Identity=30.7359307359307, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 31190; Mature: 31059

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTTSRLLATALSSAVLLGAFTLAEAGQASFVVDAQSGQVLEASNQDDLNYPASLTKMM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHH
TLYLAFEALHEGRLTWDQKLTMSENAESKEPFKLAVGAGRQVTLREAVESIVVLSANDSA
HHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEHHHHHHHEEEEECCCCH
VAIAEQLGGSEQAFAKAMTDKARQLGMTNTVFRNPSGLPDPEQVTSARDMATLGISLMRD
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FPEEFKLFSMRGFQFRGMKLRGHNNLMYRYDGVDGIKTGYTDASGYNVVTSALKDGRRVV
CHHHHHHHHCCCCEEECEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
GVVMGEKTASLRDDKMAGLLDSTLPSSSTATASTMPGKQPGATMTDAQPTK
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKTTSRLLATALSSAVLLGAFTLAEAGQASFVVDAQSGQVLEASNQDDLNYPASLTKMM
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHH
TLYLAFEALHEGRLTWDQKLTMSENAESKEPFKLAVGAGRQVTLREAVESIVVLSANDSA
HHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEEHHHHHHHEEEEECCCCH
VAIAEQLGGSEQAFAKAMTDKARQLGMTNTVFRNPSGLPDPEQVTSARDMATLGISLMRD
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
FPEEFKLFSMRGFQFRGMKLRGHNNLMYRYDGVDGIKTGYTDASGYNVVTSALKDGRRVV
CHHHHHHHHCCCCEEECEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCEEE
GVVMGEKTASLRDDKMAGLLDSTLPSSSTATASTMPGKQPGATMTDAQPTK
EEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]