Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 209549535

GI number: 209549535

Start: 1985038

End: 1986318

Strand: Direct

Name: bcr [H]

Synonym: Rleg2_1941

Alternate gene names: 209549535

Gene position: 1985038-1986318 (Clockwise)

Preceding gene: 209549534

Following gene: 209549536

Centisome position: 43.74

GC content: 61.05

Gene sequence:

>1281_bases
ATGACGCCGCCGCATAAACCGCACCAGGAAAACCAGGGCTCCCGTCGCATCGGCATGGGTTTCGGCGAGTTCGTCGTCAC
CATCGCCATCATGACCGCCAGCATCGCCATGGCCATCGACAGCATGCTGCCGGCATTGCCCAATATCGGCCATTCCCTTG
GCGTCACCAATACCAATGACGCGCAGCTGATCATCGGCGTGTTTTTTCTCGGCTTCGGCGTCTCGCAGATATTCTTCGGC
AGCCTGTCGGATACGTTCGGCCGCCGCAACATCCTGCTCGGCGGCCTGGTTTGCTACGTGATCGGCATGTTTGCCGCCGC
AGCAACCGGCAGCTTTGAAATGCTGCTCATCATGCGTTTCGTTCAGGGCGTAGGCGGTGCCGCCGTGCGCATCACCACCA
TGGCCATCGTCCGCGACTGCTTCGGCGGCCGCGAGATGGCCCGTGTCATGTCCTATGTCATGATCGTCTTCATGATCGTA
CCGATCGTTGCCCCTTCCGTCGGCCAGCTGATCATCCTTTATGCCAATTGGCACTGGATCTTCATCCTGCTCGGCGTCAC
CGCCACCATTCTCTTCGTCTGGGCGCTTCTGCGGCTGAAGGAATCGCTGCCGCCGGAAGAGCGCCTGCCGCTGTCGGTCG
GCTCCGTCGTGGATGGCTTCAAGACCGTGCTTACCAACCGCATCACCTGCGGCTACATGATCGGCCTCACCATGTTCACC
GGCGTCATCAGCGCCTATGTGATCTCGGTGCAGCAGGTCTTCGGCGAGGTCTATGGCCTTGGTGATTGGCTGCCGATCGC
CTTTGCCGCCACCGCCGGCGGCATCGCCGTCGCTAATTTCGCCAACGGTTTCTTCGTCCGAAAATTCGGCATGCGCCGTA
TTTCGCACGCCGCTTTGCTGATCTTCACCGCGCTGTCGGCTCTGGGTTTCATCATCGCAATGGCCGGCAAGCCGGATTTC
ACCGTCGCTTACAGCATCTTCACCATCGTGCTGATGATGTTTGCCGTCATCGCCACCAATTTTACCGCCATCAGCCTCGA
GCCGATGGGCAATCTCGCCGGCACAGCAACCGCCGTCACCGGCTTCGTCTCGACGACAGCAGGCGCCATTCTCGGCGGCC
TGGTCGGCCAGATGTTCAACGGCACGGTGCAGCCGCTCTTCGGCGGCTTTGCACTCTTCGGTCTGGTGACGATCGCAGCC
ACTCTTTGGGCTGAGAACGGCAAGCTCTTCACCCATCCCGGCGACAGCCCGCAGCTCGATCCGGGCGCCGCCCATTTCTG
A

Upstream 100 bases:

>100_bases
TCGCCGAAAAGGGCCGCCTCTTCCAACAGCAGAACCCGGCCGTCTGAACGGCCCACCTCCTCTGAACGGCCTACCTCCTC
CGCATCCAGGATTTCACCAC

Downstream 100 bases:

>100_bases
TGGCCGAAAACGATTCGGCGCAGTGTGCGAAAGGCATTCAATGGACGACCCCATTCTGATCAGACCTTACGCGCCAAGCG
ACGTGGATGCGACAATTGAC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTPPHKPHQENQGSRRIGMGFGEFVVTIAIMTASIAMAIDSMLPALPNIGHSLGVTNTNDAQLIIGVFFLGFGVSQIFFG
SLSDTFGRRNILLGGLVCYVIGMFAAAATGSFEMLLIMRFVQGVGGAAVRITTMAIVRDCFGGREMARVMSYVMIVFMIV
PIVAPSVGQLIILYANWHWIFILLGVTATILFVWALLRLKESLPPEERLPLSVGSVVDGFKTVLTNRITCGYMIGLTMFT
GVISAYVISVQQVFGEVYGLGDWLPIAFAATAGGIAVANFANGFFVRKFGMRRISHAALLIFTALSALGFIIAMAGKPDF
TVAYSIFTIVLMMFAVIATNFTAISLEPMGNLAGTATAVTGFVSTTAGAILGGLVGQMFNGTVQPLFGGFALFGLVTIAA
TLWAENGKLFTHPGDSPQLDPGAAHF

Sequences:

>Translated_426_residues
MTPPHKPHQENQGSRRIGMGFGEFVVTIAIMTASIAMAIDSMLPALPNIGHSLGVTNTNDAQLIIGVFFLGFGVSQIFFG
SLSDTFGRRNILLGGLVCYVIGMFAAAATGSFEMLLIMRFVQGVGGAAVRITTMAIVRDCFGGREMARVMSYVMIVFMIV
PIVAPSVGQLIILYANWHWIFILLGVTATILFVWALLRLKESLPPEERLPLSVGSVVDGFKTVLTNRITCGYMIGLTMFT
GVISAYVISVQQVFGEVYGLGDWLPIAFAATAGGIAVANFANGFFVRKFGMRRISHAALLIFTALSALGFIIAMAGKPDF
TVAYSIFTIVLMMFAVIATNFTAISLEPMGNLAGTATAVTGFVSTTAGAILGGLVGQMFNGTVQPLFGGFALFGLVTIAA
TLWAENGKLFTHPGDSPQLDPGAAHF
>Mature_425_residues
TPPHKPHQENQGSRRIGMGFGEFVVTIAIMTASIAMAIDSMLPALPNIGHSLGVTNTNDAQLIIGVFFLGFGVSQIFFGS
LSDTFGRRNILLGGLVCYVIGMFAAAATGSFEMLLIMRFVQGVGGAAVRITTMAIVRDCFGGREMARVMSYVMIVFMIVP
IVAPSVGQLIILYANWHWIFILLGVTATILFVWALLRLKESLPPEERLPLSVGSVVDGFKTVLTNRITCGYMIGLTMFTG
VISAYVISVQQVFGEVYGLGDWLPIAFAATAGGIAVANFANGFFVRKFGMRRISHAALLIFTALSALGFIIAMAGKPDFT
VAYSIFTIVLMMFAVIATNFTAISLEPMGNLAGTATAVTGFVSTTAGAILGGLVGQMFNGTVQPLFGGFALFGLVTIAAT
LWAENGKLFTHPGDSPQLDPGAAHF

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=400, Percent_Identity=27.5, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1790146, Length=327, Percent_Identity=25.9938837920489, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI48994889, Length=159, Percent_Identity=29.559748427673, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790794, Length=192, Percent_Identity=25.5208333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082312, Length=352, Percent_Identity=23.5795454545455, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790195, Length=140, Percent_Identity=30.7142857142857, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI87081983, Length=150, Percent_Identity=28.6666666666667, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45260; Mature: 45129

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPPHKPHQENQGSRRIGMGFGEFVVTIAIMTASIAMAIDSMLPALPNIGHSLGVTNTND
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AQLIIGVFFLGFGVSQIFFGSLSDTFGRRNILLGGLVCYVIGMFAAAATGSFEMLLIMRF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VQGVGGAAVRITTMAIVRDCFGGREMARVMSYVMIVFMIVPIVAPSVGQLIILYANWHWI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
FILLGVTATILFVWALLRLKESLPPEERLPLSVGSVVDGFKTVLTNRITCGYMIGLTMFT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GVISAYVISVQQVFGEVYGLGDWLPIAFAATAGGIAVANFANGFFVRKFGMRRISHAALL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
IFTALSALGFIIAMAGKPDFTVAYSIFTIVLMMFAVIATNFTAISLEPMGNLAGTATAVT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
GFVSTTAGAILGGLVGQMFNGTVQPLFGGFALFGLVTIAATLWAENGKLFTHPGDSPQLD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PGAAHF
CCCCCC
>Mature Secondary Structure 
TPPHKPHQENQGSRRIGMGFGEFVVTIAIMTASIAMAIDSMLPALPNIGHSLGVTNTND
CCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AQLIIGVFFLGFGVSQIFFGSLSDTFGRRNILLGGLVCYVIGMFAAAATGSFEMLLIMRF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VQGVGGAAVRITTMAIVRDCFGGREMARVMSYVMIVFMIVPIVAPSVGQLIILYANWHWI
HHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHH
FILLGVTATILFVWALLRLKESLPPEERLPLSVGSVVDGFKTVLTNRITCGYMIGLTMFT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
GVISAYVISVQQVFGEVYGLGDWLPIAFAATAGGIAVANFANGFFVRKFGMRRISHAALL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
IFTALSALGFIIAMAGKPDFTVAYSIFTIVLMMFAVIATNFTAISLEPMGNLAGTATAVT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHH
GFVSTTAGAILGGLVGQMFNGTVQPLFGGFALFGLVTIAATLWAENGKLFTHPGDSPQLD
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PGAAHF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]