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The map label for this gene is purB [H]

Identifier: 209549515

GI number: 209549515

Start: 1966499

End: 1967806

Strand: Direct

Name: purB [H]

Synonym: Rleg2_1921

Alternate gene names: 209549515

Gene position: 1966499-1967806 (Clockwise)

Preceding gene: 209549514

Following gene: 209549518

Centisome position: 43.33

GC content: 63.15

Gene sequence:

>1308_bases
ATGATCCCGCGTTATTCCCGGCCCGAAATGGTCGCCATCTGGTCTCCCGAAACCAAGTTCCGCATCTGGTTCGAGATCGA
GGCGCATGCCTGCGACGCGTTGGCCGAACTCGGCGTCATCCCGAAATCGGCGGCAAAGACGATCTGGGAAAAGGGCGGTG
CGGCCACCTTTGACGTCGCTCGCATTGACGAGATCGAAGCGGTCACCAAACATGACGTCATCGCCTTCCTCACCCACCTC
GCCGAGATCGTCGGCCCGGATGCACGCTTCGTCCACCAGGGCATGACCTCATCCGACGTGCTCGACACCTGCTTCAACGT
CCAGTTGGTGCGCGCCACCGACATCCTGATTGCCGATATTGACCGCCTGCTCGCAGCGCTCAAAACCCGCGCCTTCGAAC
ATAAGGACACTGTCACCATCGGCCGTTCGCACGGCATCCACGCCGAGCCCACCACCTTCGGCGTCAAGCTGGCGCTTGCC
TATGCCGAATTCGAGCGCTGCCGCCAGCGCCTCGTCGCCGCCCGTGAGGAAGTCGCGACCTGCGCCATCTCAGGCGCCGT
CGGCACCTTCGCCAACATCGACCCACGTGTCGAAGAACATGTCGCCGAGGCGCTTGGCCTGAAGGCCGAGCCGGTCTCGA
CCCAGGTTATCCCGCGCGACCGCCACGCCATGTACTTCGCCACCCTCGGTGTCGTCGCCTCGTCGATCGAGCGGCTCGCG
ACCGAGATCCGCCACCTGCAGCGCACCGAGGTGCTGGAAGCCGAGGAATATTTCTCGCCCGGCCAGAAGGGCTCCTCGGC
CATGCCCCACAAGCGCAACCCGGTGCTGACCGAAAACCTCACCGGCCTCGCCCGCATGGTCCGCTCCTACGCCTTGCCGG
CCATGGAAAACGTCGCGCTCTGGCACGAGCGCGATATCTCCCACTCCTCGGTCGAACGCATGATCGGCCCCGACGCCACC
GTCACCCTCGATTTCGCCCTCTCGCGTCTCGCCGGCGTCATCGAAAAGCTGCTGGTCTACCCTGACAACATGGAGAAGAA
CCTCAACAAATTCCGCGGCCTCGTCCACTCCCAGCGCGTCCTGCTGGCACTGACCCAAGCCGGCACTTCCCGCGAAGACG
CCTACCGCCTGGTGCAGCGCAACGCCATGAAGGTTTGGGAGCAGGGTAAGGACTTTCTGGAAGAGCTGCTTGCGGATGCT
GAGGTTCGGGCCGCGCTTTCCGAAGAGGATATTCGCGAGAAGTTCGATCTTGGGTATCACACCAAGCATGTGGACACGAT
TTTCCACCGAGTGTTTGGCGAGGCGTGA

Upstream 100 bases:

>100_bases
CTACTATCTTGCCAAACGCATGCGTTGAGATTGCCCTCCGTCTTTGCTACAGGGGCGCAGACATTCACGCATGAACCTCT
CGAAGTGAAGGCAGCCGCCC

Downstream 100 bases:

>100_bases
GTTCTCCAAATCGATAATATTGCGGCGCCCTTACGGCGCCGTTTTCATGTCTGCTTCCATACTGTTATCGCAGTTGTCGC
GCCTGCGCTCGCCAATATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAKTIWEKGGAATFDVARIDEIEAVTKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCFNVQLVRATDILIADIDRLLAALKTRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFERCRQRLVAAREEVATCAISGAVGTFANIDPRVEEHVAEALGLKAEPVSTQVIPRDRHAMYFATLGVVASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALSRLAGVIEKLLVYPDNMEKNLNKFRGLVHSQRVLLALTQAGTSREDAYRLVQRNAMKVWEQGKDFLEELLADA
EVRAALSEEDIREKFDLGYHTKHVDTIFHRVFGEA

Sequences:

>Translated_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAKTIWEKGGAATFDVARIDEIEAVTKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCFNVQLVRATDILIADIDRLLAALKTRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFERCRQRLVAAREEVATCAISGAVGTFANIDPRVEEHVAEALGLKAEPVSTQVIPRDRHAMYFATLGVVASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALSRLAGVIEKLLVYPDNMEKNLNKFRGLVHSQRVLLALTQAGTSREDAYRLVQRNAMKVWEQGKDFLEELLADA
EVRAALSEEDIREKFDLGYHTKHVDTIFHRVFGEA
>Mature_435_residues
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAKTIWEKGGAATFDVARIDEIEAVTKHDVIAFLTHL
AEIVGPDARFVHQGMTSSDVLDTCFNVQLVRATDILIADIDRLLAALKTRAFEHKDTVTIGRSHGIHAEPTTFGVKLALA
YAEFERCRQRLVAAREEVATCAISGAVGTFANIDPRVEEHVAEALGLKAEPVSTQVIPRDRHAMYFATLGVVASSIERLA
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVALWHERDISHSSVERMIGPDAT
VTLDFALSRLAGVIEKLLVYPDNMEKNLNKFRGLVHSQRVLLALTQAGTSREDAYRLVQRNAMKVWEQGKDFLEELLADA
EVRAALSEEDIREKFDLGYHTKHVDTIFHRVFGEA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=415, Percent_Identity=28.433734939759, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=28.3464566929134, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787376, Length=349, Percent_Identity=29.2263610315186, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=398, Percent_Identity=26.6331658291457, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564234, Length=363, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=427, Percent_Identity=28.5714285714286, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24647570, Length=404, Percent_Identity=27.2277227722772, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48574; Mature: 48574

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAKTIWEKGGAATFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEHH
RIDEIEAVTKHDVIAFLTHLAEIVGPDARFVHQGMTSSDVLDTCFNVQLVRATDILIADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DRLLAALKTRAFEHKDTVTIGRSHGIHAEPTTFGVKLALAYAEFERCRQRLVAAREEVAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAEALGLKAEPVSTQVIPRDRHAMYFATLGVVASSIERLA
HHHHCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALSRLAGVIEKLLVYPDNMEKNLNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLALTQAGTSREDAYRLVQRNAMKVWEQGKDFLEELLADAEVRAALSEEDIREKFDLGYH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFHRVFGEA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMVAIWSPETKFRIWFEIEAHACDALAELGVIPKSAAKTIWEKGGAATFDVA
CCCCCCCCCEEEEECCCCEEEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEHH
RIDEIEAVTKHDVIAFLTHLAEIVGPDARFVHQGMTSSDVLDTCFNVQLVRATDILIADI
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHCEEEEHHHHHHHHHH
DRLLAALKTRAFEHKDTVTIGRSHGIHAEPTTFGVKLALAYAEFERCRQRLVAAREEVAT
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANIDPRVEEHVAEALGLKAEPVSTQVIPRDRHAMYFATLGVVASSIERLA
HHHHCCHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEYFSPGQKGSSAMPHKRNPVLTENLTGLARMVRSYALPAMENVAL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALSRLAGVIEKLLVYPDNMEKNLNKFRGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LLALTQAGTSREDAYRLVQRNAMKVWEQGKDFLEELLADAEVRAALSEEDIREKFDLGYH
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFHRVFGEA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]