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The map label for this gene is adhI [H]

Identifier: 209549469

GI number: 209549469

Start: 1922297

End: 1923424

Strand: Direct

Name: adhI [H]

Synonym: Rleg2_1875

Alternate gene names: 209549469

Gene position: 1922297-1923424 (Clockwise)

Preceding gene: 209549468

Following gene: 209549470

Centisome position: 42.36

GC content: 62.59

Gene sequence:

>1128_bases
ATGGACGTTCGCGCCGCCGTAGCCGTTCAGGCCGGAAAACCGCTCGAAGTGATGACCGTGCAGCTGGAAGGCCCGCGGGC
CGGCGAAGTGCTGGTCGAGGTCAAGGCGACGGGCATCTGCCACACCGACGATTTCACGCTGTCGGGCGCCGATCCGGAAG
GTCTGTTTCCGGCGATCCTCGGCCATGAGGGTGCCGGTATCGTCGTCGATGTCGGGCCCGGCGTCACCTCGGTCAAGAAA
GGCGACCATGTCATTCCGCTCTACACACCGGAATGCCGCGAGTGTTACTCCTGCCTGTCCCGCAAGACCAATCTCTGCAC
CGCGATCCGCTCGACCCAGGGCCAGGGTGTGATGCCGGACGGCACCTCGCGCTTTTCGATCGGCAAGGACAAGATCCACC
ACTATATGGGCTGCTCGACCTTCGCCAATTTCACCGTGCTGCCGGAGATCGCCCTTGCCAAGGTCAATCCCGACGCCCCC
TTCGACAAGATCTGCTATATCGGCTGCGGCGTCACCACCGGCATCGGCGCGGTGATCAACACCGCCAAGGTCGAGATCGG
TTCGACGGCGATCGTCTTCGGTCTCGGCGGCATCGGCCTCAACGTGCTGCAGGGCCTCAGGCTTGCCGGCGCCGACATGA
TCATCGGCGTCGACATCAACAATGACCGCAAGGCCTGGGGCGAAAAGTTCGGCATGACGCATTTCGTCAATCCGAAGGAG
GTCGGTGACGACATCGTGCCTCATCTCGTCAATATGACGAAGCGCAATGGCGACCTGATCGGCGGCGCCGACTATACATT
CGACTGCACCGGCAACACCAAGGTGATGCGCCAGGCGCTTGAGGCCTCCCACCGCGGCTGGGGCAAATCGATCATCATCG
GCGTTGCCGGCGCCGGCCAGGAGATCTCGACGCGTCCGTTCCAGCTGGTCACCGGCCGCAACTGGATGGGCACCGCCTTC
GGCGGCGCGCGGGGGCGTACCGACGTGCCGAAGATTGTCGACTGGTACATGCAGGGCAAAATCCAGATCGACCCGATGAT
CACCCACACCATGCCACTCCAAGACATCAACAAGGGCTTCGACATGATGCACAAGGGTGAAAGCATCCGCGGCGTGGTGG
TCTATTGA

Upstream 100 bases:

>100_bases
CTGAACCAAAAGCGGCGCCGGCCAGCTGTTTTCTCTTGAGCCGGCCTTCCCGGCCATGCCAAGGTCTGCGTCAAATAAAG
TAAAAAGGGAGATTGGGAAG

Downstream 100 bases:

>100_bases
TGCCTTCCAAGACCTACACGGCCGACCTCAAGAGCGTCGATGAGCTCCTCGCAGGGGAGCTCTACGACCTCTTGAAAATG
CGCGTCGATGTTTTCGTCGT

Product: S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPRAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCLSRKTNLCTAIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINNDRKAWGEKFGMTHFVNPKE
VGDDIVPHLVNMTKRNGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLQDINKGFDMMHKGESIRGVVVY

Sequences:

>Translated_375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPRAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCLSRKTNLCTAIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINNDRKAWGEKFGMTHFVNPKE
VGDDIVPHLVNMTKRNGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLQDINKGFDMMHKGESIRGVVVY
>Mature_375_residues
MDVRAAVAVQAGKPLEVMTVQLEGPRAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCLSRKTNLCTAIRSTQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANFTVLPEIALAKVNPDAP
FDKICYIGCGVTTGIGAVINTAKVEIGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINNDRKAWGEKFGMTHFVNPKE
VGDDIVPHLVNMTKRNGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLQDINKGFDMMHKGESIRGVVVY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=56.0321715817694, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=42.6315789473684, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=42.9333333333333, Blast_Score=321, Evalue=9e-88,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=42.6666666666667, Blast_Score=317, Evalue=8e-87,
Organism=Homo sapiens, GI262073058, Length=376, Percent_Identity=41.4893617021277, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI71743840, Length=376, Percent_Identity=41.4893617021277, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=41.8666666666667, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=40.7008086253369, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI4501939, Length=364, Percent_Identity=40.9340659340659, Blast_Score=296, Evalue=1e-80,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=57.8666666666667, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1788073, Length=372, Percent_Identity=26.8817204301075, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787863, Length=365, Percent_Identity=22.4657534246575, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=24.2268041237113, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI226510992, Length=374, Percent_Identity=24.5989304812834, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786825, Length=383, Percent_Identity=23.7597911227154, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081918, Length=372, Percent_Identity=26.6129032258064, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=379, Percent_Identity=53.2981530343008, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71997431, Length=381, Percent_Identity=53.0183727034121, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=53.935860058309, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17562584, Length=378, Percent_Identity=25.9259259259259, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71988145, Length=374, Percent_Identity=24.5989304812834, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562582, Length=382, Percent_Identity=24.6073298429319, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=54.6916890080429, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=26.7217630853994, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6319955, Length=359, Percent_Identity=26.7409470752089, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6319621, Length=377, Percent_Identity=27.5862068965517, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323099, Length=375, Percent_Identity=25.8666666666667, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319257, Length=358, Percent_Identity=24.8603351955307, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319258, Length=280, Percent_Identity=28.2142857142857, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=354, Percent_Identity=24.5762711864407, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=368, Percent_Identity=25, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323961, Length=373, Percent_Identity=24.9329758713137, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=57.4866310160428, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 40020; Mature: 40020

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLEGPRAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCLSRKTNLCTAIRSTQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFANFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEIGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINNDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCEECCCHHH
VGDDIVPHLVNMTKRNGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQ
HCHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLQDINKGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEECCCHHHHHHCH
DMMHKGESIRGVVVY
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLEGPRAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCEEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCLSRKTNLCTAIRSTQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFANFTVLPEIALAKVNPDAPFDKICYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEIGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINNDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEEECCCHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHCCCEECCCHHH
VGDDIVPHLVNMTKRNGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSIIIGVAGAGQ
HCHHHHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLQDINKGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEECCCHHHHHHCH
DMMHKGESIRGVVVY
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]