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The map label for this gene is dacF [H]

Identifier: 209549453

GI number: 209549453

Start: 1906477

End: 1908030

Strand: Direct

Name: dacF [H]

Synonym: Rleg2_1859

Alternate gene names: 209549453

Gene position: 1906477-1908030 (Clockwise)

Preceding gene: 209549452

Following gene: 209549454

Centisome position: 42.01

GC content: 64.54

Gene sequence:

>1554_bases
GTGTCAAGGTCAGTCTCCTCCGTATCATCGTCCCGATCCGGCAGCTTTTTTGCAAAGCTCATGGCGATCCTTTCGGTGGC
CCTCACGGTCGTCCTGGTCGATTCGGTCAGTGCCGAAGCGGAAGCGGCGAATTCGAAATATGCGGGCATCGTCGTCGACG
CCAAGACCGGCAACGTTCTCTACAGCGAGAATGCCGACCGGCTGCAATATCCCGCCTCCCTGACCAAGATGATGACCATC
TACATGACCTTCGAGGCGCTGGAGCAGGGTCGCATCCGTCTCGATACGCCGGTTCCCTTCTCTGCCCATGCGGCAGCTCA
GGCGCCGACCAAGCTCGGCGTTCGCGCCGGCGGCACGATCACCGTCGAACAGGGCATTCTCGGCCTCGTCACCCTGTCGG
CCAATGATGCCGCAACCGCGCTCGGCGAAATGCTCGGCGGCGGCAGCGAGGATCGTTTCGCCCAGCTGATGACCGCCAAG
GCGCATGCGCTCGGCATGACCCGCACCACCTATCGCAACGCCAACGGCCTGCCGAATACGGCGCAGATGACGACGGCGCG
CGATCAGGCCCGTCTCGGCATCGCCCTTCGCCAGCATTTTCCGCAATATTACGGCTACTTCTCCACCCGCGCCTTCAAGT
TCGGCACCCGCACGATCCGCAGCCACAATCGCCTCGTCGGTTCGGTGCGCGGCGTCGACGGCATCAAGACCGGATATACC
CGCGCTGCCGGCTTCAATCTGGTGAGCTCCGTGCAGGTCGACGGCAAGTCGATCGTCGGTGTCGTGCTCGGCGGCGCCTC
GACGCCTGCCCGCGACGCCCAGATGCGCAATCTGATCGCCACCTATCTGCCGAAGGCATCGAGCCGCGGCGGATCGTCGG
CCTTGATCGCTGAAGCGGCCCCTGCCCCGGCAATGATCGTGACACCGGCTCCGGTCATGCCGCAGAAGGCCCAGCCGCAA
GTGGCAAAGACGATCACCGCAGCGCAGCCGCCGGTTTCCGCAGCCGCCGCCGATCTCAGCCTGCCGCATAAAGGCCCGCT
GCCGGATGCCCGTTATCAGGTCGCCGAGACCGAAGTGGCCTATACCGAAACCGCTCAGCCGAAATCCGACAATCCGCTGG
TAGCCCAGCCGATGCCGGCGCCGACCAAGGTCAAGACCACAACCTTCAAGCAGCAGGCATCGCTCACCGCGCCGAAGCCG
GCACCCGCTTCCGTACAGCCGGATCAGGTCGATACGACGGTCGACAACGTCACCACTGCTTCGACGACTCCAACCTCTGC
CGGCGCCGCTTCAAACAATAATGGCCCGACCGGCTGGGTCGTGCAGGTCGGCGTTTCGCCGAGCCGACAGATGGCCATGG
ATCTTCTGGAGAGCGCCAAGAGCAAGGGCGGCAAGGCGCTGGCCTCGGCAAAGCCTTTCGCCGTCGCCTATGCCGCCGGC
GGTGACCAGCTTTACCGCGCCCGCTTCGGCGGTTTCGACGACCAGCGCGATGCGGTCAATGCCTGCAAGGCCTTGAAGAA
GGCCGGTATCAAGTGCTGGGCGGCTGCCCAATGA

Upstream 100 bases:

>100_bases
ATCGGTAAATTCAAAAGACGGGCTGTACTGCGGGAGGCGTGCCGTCCCGACGCCGAAATCCGAATGCCAAATATCTGAAA
TGCAAGCGAAGAGTGGAATA

Downstream 100 bases:

>100_bases
GCTATTCGGTGCTGCCTGCAACATGGCCGGCGGCATCGGTAACGGCCGGAATCGGTGTTTGCGTTTCCGGCCATCGTTCT
TGAACTCGATCAGTATGGCG

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSRSVSSVSSSRSGSFFAKLMAILSVALTVVLVDSVSAEAEAANSKYAGIVVDAKTGNVLYSENADRLQYPASLTKMMTI
YMTFEALEQGRIRLDTPVPFSAHAAAQAPTKLGVRAGGTITVEQGILGLVTLSANDAATALGEMLGGGSEDRFAQLMTAK
AHALGMTRTTYRNANGLPNTAQMTTARDQARLGIALRQHFPQYYGYFSTRAFKFGTRTIRSHNRLVGSVRGVDGIKTGYT
RAAGFNLVSSVQVDGKSIVGVVLGGASTPARDAQMRNLIATYLPKASSRGGSSALIAEAAPAPAMIVTPAPVMPQKAQPQ
VAKTITAAQPPVSAAAADLSLPHKGPLPDARYQVAETEVAYTETAQPKSDNPLVAQPMPAPTKVKTTTFKQQASLTAPKP
APASVQPDQVDTTVDNVTTASTTPTSAGAASNNNGPTGWVVQVGVSPSRQMAMDLLESAKSKGGKALASAKPFAVAYAAG
GDQLYRARFGGFDDQRDAVNACKALKKAGIKCWAAAQ

Sequences:

>Translated_517_residues
MSRSVSSVSSSRSGSFFAKLMAILSVALTVVLVDSVSAEAEAANSKYAGIVVDAKTGNVLYSENADRLQYPASLTKMMTI
YMTFEALEQGRIRLDTPVPFSAHAAAQAPTKLGVRAGGTITVEQGILGLVTLSANDAATALGEMLGGGSEDRFAQLMTAK
AHALGMTRTTYRNANGLPNTAQMTTARDQARLGIALRQHFPQYYGYFSTRAFKFGTRTIRSHNRLVGSVRGVDGIKTGYT
RAAGFNLVSSVQVDGKSIVGVVLGGASTPARDAQMRNLIATYLPKASSRGGSSALIAEAAPAPAMIVTPAPVMPQKAQPQ
VAKTITAAQPPVSAAAADLSLPHKGPLPDARYQVAETEVAYTETAQPKSDNPLVAQPMPAPTKVKTTTFKQQASLTAPKP
APASVQPDQVDTTVDNVTTASTTPTSAGAASNNNGPTGWVVQVGVSPSRQMAMDLLESAKSKGGKALASAKPFAVAYAAG
GDQLYRARFGGFDDQRDAVNACKALKKAGIKCWAAAQ
>Mature_516_residues
SRSVSSVSSSRSGSFFAKLMAILSVALTVVLVDSVSAEAEAANSKYAGIVVDAKTGNVLYSENADRLQYPASLTKMMTIY
MTFEALEQGRIRLDTPVPFSAHAAAQAPTKLGVRAGGTITVEQGILGLVTLSANDAATALGEMLGGGSEDRFAQLMTAKA
HALGMTRTTYRNANGLPNTAQMTTARDQARLGIALRQHFPQYYGYFSTRAFKFGTRTIRSHNRLVGSVRGVDGIKTGYTR
AAGFNLVSSVQVDGKSIVGVVLGGASTPARDAQMRNLIATYLPKASSRGGSSALIAEAAPAPAMIVTPAPVMPQKAQPQV
AKTITAAQPPVSAAAADLSLPHKGPLPDARYQVAETEVAYTETAQPKSDNPLVAQPMPAPTKVKTTTFKQQASLTAPKPA
PASVQPDQVDTTVDNVTTASTTPTSAGAASNNNGPTGWVVQVGVSPSRQMAMDLLESAKSKGGKALASAKPFAVAYAAGG
DQLYRARFGGFDDQRDAVNACKALKKAGIKCWAAAQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=237, Percent_Identity=33.7552742616034, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1787062, Length=250, Percent_Identity=34.4, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI87082022, Length=238, Percent_Identity=32.3529411764706, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082054, Length=248, Percent_Identity=31.4516129032258, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53800; Mature: 53668

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSVSSVSSSRSGSFFAKLMAILSVALTVVLVDSVSAEAEAANSKYAGIVVDAKTGNVL
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEE
YSENADRLQYPASLTKMMTIYMTFEALEQGRIRLDTPVPFSAHAAAQAPTKLGVRAGGTI
ECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCCCHHHCCCCCCEE
TVEQGILGLVTLSANDAATALGEMLGGGSEDRFAQLMTAKAHALGMTRTTYRNANGLPNT
EECCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCC
AQMTTARDQARLGIALRQHFPQYYGYFSTRAFKFGTRTIRSHNRLVGSVRGVDGIKTGYT
HHHHHHHHHHHHHHHHHHHCHHHHCHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
RAAGFNLVSSVQVDGKSIVGVVLGGASTPARDAQMRNLIATYLPKASSRGGSSALIAEAA
HHHCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PAPAMIVTPAPVMPQKAQPQVAKTITAAQPPVSAAAADLSLPHKGPLPDARYQVAETEVA
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEHHHCEEE
YTETAQPKSDNPLVAQPMPAPTKVKTTTFKQQASLTAPKPAPASVQPDQVDTTVDNVTTA
EECCCCCCCCCCEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHCCEEC
STTPTSAGAASNNNGPTGWVVQVGVSPSRQMAMDLLESAKSKGGKALASAKPFAVAYAAG
CCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECC
GDQLYRARFGGFDDQRDAVNACKALKKAGIKCWAAAQ
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
SRSVSSVSSSRSGSFFAKLMAILSVALTVVLVDSVSAEAEAANSKYAGIVVDAKTGNVL
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEECCCCCEE
YSENADRLQYPASLTKMMTIYMTFEALEQGRIRLDTPVPFSAHAAAQAPTKLGVRAGGTI
ECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHCCCHHHCCCCCCEE
TVEQGILGLVTLSANDAATALGEMLGGGSEDRFAQLMTAKAHALGMTRTTYRNANGLPNT
EECCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCC
AQMTTARDQARLGIALRQHFPQYYGYFSTRAFKFGTRTIRSHNRLVGSVRGVDGIKTGYT
HHHHHHHHHHHHHHHHHHHCHHHHCHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCC
RAAGFNLVSSVQVDGKSIVGVVLGGASTPARDAQMRNLIATYLPKASSRGGSSALIAEAA
HHHCCCCCEEEEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
PAPAMIVTPAPVMPQKAQPQVAKTITAAQPPVSAAAADLSLPHKGPLPDARYQVAETEVA
CCCEEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCEEHHHCEEE
YTETAQPKSDNPLVAQPMPAPTKVKTTTFKQQASLTAPKPAPASVQPDQVDTTVDNVTTA
EECCCCCCCCCCEEECCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHCCEEC
STTPTSAGAASNNNGPTGWVVQVGVSPSRQMAMDLLESAKSKGGKALASAKPFAVAYAAG
CCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECC
GDQLYRARFGGFDDQRDAVNACKALKKAGIKCWAAAQ
CCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]