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The map label for this gene is tgt [H]

Identifier: 209549348

GI number: 209549348

Start: 1793075

End: 1794205

Strand: Direct

Name: tgt [H]

Synonym: Rleg2_1752

Alternate gene names: 209549348

Gene position: 1793075-1794205 (Clockwise)

Preceding gene: 209549347

Following gene: 209549359

Centisome position: 39.51

GC content: 64.01

Gene sequence:

>1131_bases
ATGACAGAGAACTTCCAATTCACGTTGAAGAAGACCGATGCCGGCGCGCGCCTCGGCGAAATCTCCATGCCGCGCGGCAC
GATCCGCACGCCGGCCTTCATGCCTGTGGGGACCGTCGGCACGGTCAAGGCGATGTATCTCGACCAGGTGCGCGAAACCG
GCGCCGACATCATCCTCGGCAATACCTATCACCTGATGCTGCGCCCCAGCGCCGAGCGCGTCGCCCGCCTCGGTGGCCTG
CACAAGCTGATCCGCTGGGAGCACCCGATCCTGACGGATTCCGGCGGCTTCCAGGTCATGTCGCTCTCCGGTCTGCGCAA
GCTCGACGAGCAGGGCGTCACCTTCAAGTCGCATGTCGACGGCAGCCTGCACCATATGTCGCCGGAGCGTTCCATCGAAA
TCCAGGGCCTTCTCGGCTCCGACATCCAGATGCAGCTCGACGAATGCGTGGCACTGCCGGCTGAGCCGCGGGAGATTGAG
CGCGCCATGGAAATGTCGCTGCGTTGGGCCGAGCGCTGCCGGGTCGCCTTCGGCGAGCAGCCTGGCAAGGCGATGTTCGG
CATCGTCCAGGGAGGCGATATTCCCGCTTTGCGCGTTCGTTCGGCGCAGGCGCTGAGCGAGCTCGACCTCAAGGGTTATG
CCATCGGCGGCCTTGCCGTCGGCGAGCCGCAGGATGTCATGCTGCAGATGCTGGACACGACACTGCCGGTGCTCCCCGGG
GAAAAGCCGCGTTACCTCATGGGCGTCGGCACGCCGGATGATATCCTGAAATCGGTTGCCCGCGGCATCGACATGTTCGA
CTGCGTCATGCCGACCCGTTCCGGCCGCCACGGCCTGGCCTTTACCCGCCGCGGCAAGGTCAATATCCGCAATGCCCGTC
ATGCCGAGGATATGCGCCCGCTCGACGATCAGTCGAACTGCCCGGCCTCGCGCGATTATTCACGCGCCTATCTGCACCAT
CTCGTCCGCTCCAACGAAGCACTCGGCGGCATGTTGCTCTCCTGGAACAATCTCGCCTATTACCAGGAACTGATGCAGGG
CATCCGCAAGGCGATCGCCGAAGGCCGCTTCGCCGATTTCATGGCGGAGACGATGGAGGAATGGGCAAGAGGCGATCTCG
ACCCGGTCTAA

Upstream 100 bases:

>100_bases
CAGGCTTCGAAACCATGCACGCGGCCTATAAACACGCCATTTCGACAGGCTACCGCTTCTACTCCTACGGCGACGCGAGC
CTTCTTTTCCGGAAAGACAA

Downstream 100 bases:

>100_bases
GGCTAGCTCGCTCCAGCGCGGCTCTCCGCGGGAAGCTCAGATGCTGGTCTTGGCGCAATCATTGTTTTGAGCTTTTTGCG
CACCGGCTCGTCGAAGTGAA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTENFQFTLKKTDAGARLGEISMPRGTIRTPAFMPVGTVGTVKAMYLDQVRETGADIILGNTYHLMLRPSAERVARLGGL
HKLIRWEHPILTDSGGFQVMSLSGLRKLDEQGVTFKSHVDGSLHHMSPERSIEIQGLLGSDIQMQLDECVALPAEPREIE
RAMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDIPALRVRSAQALSELDLKGYAIGGLAVGEPQDVMLQMLDTTLPVLPG
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGKVNIRNARHAEDMRPLDDQSNCPASRDYSRAYLHH
LVRSNEALGGMLLSWNNLAYYQELMQGIRKAIAEGRFADFMAETMEEWARGDLDPV

Sequences:

>Translated_376_residues
MTENFQFTLKKTDAGARLGEISMPRGTIRTPAFMPVGTVGTVKAMYLDQVRETGADIILGNTYHLMLRPSAERVARLGGL
HKLIRWEHPILTDSGGFQVMSLSGLRKLDEQGVTFKSHVDGSLHHMSPERSIEIQGLLGSDIQMQLDECVALPAEPREIE
RAMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDIPALRVRSAQALSELDLKGYAIGGLAVGEPQDVMLQMLDTTLPVLPG
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGKVNIRNARHAEDMRPLDDQSNCPASRDYSRAYLHH
LVRSNEALGGMLLSWNNLAYYQELMQGIRKAIAEGRFADFMAETMEEWARGDLDPV
>Mature_375_residues
TENFQFTLKKTDAGARLGEISMPRGTIRTPAFMPVGTVGTVKAMYLDQVRETGADIILGNTYHLMLRPSAERVARLGGLH
KLIRWEHPILTDSGGFQVMSLSGLRKLDEQGVTFKSHVDGSLHHMSPERSIEIQGLLGSDIQMQLDECVALPAEPREIER
AMEMSLRWAERCRVAFGEQPGKAMFGIVQGGDIPALRVRSAQALSELDLKGYAIGGLAVGEPQDVMLQMLDTTLPVLPGE
KPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGKVNIRNARHAEDMRPLDDQSNCPASRDYSRAYLHHL
VRSNEALGGMLLSWNNLAYYQELMQGIRKAIAEGRFADFMAETMEEWARGDLDPV

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=43.1428571428571, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI13375872, Length=267, Percent_Identity=23.9700374531835, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1786607, Length=366, Percent_Identity=51.6393442622951, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=41.5254237288136, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=135, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920510, Length=383, Percent_Identity=43.8642297650131, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI21355311, Length=247, Percent_Identity=24.6963562753036, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41738; Mature: 41607

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENFQFTLKKTDAGARLGEISMPRGTIRTPAFMPVGTVGTVKAMYLDQVRETGADIILG
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPSAERVARLGGLHKLIRWEHPILTDSGGFQVMSLSGLRKLDEQGVTFKSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCC
GSLHHMSPERSIEIQGLLGSDIQMQLDECVALPAEPREIERAMEMSLRWAERCRVAFGEQ
CCHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDIPALRVRSAQALSELDLKGYAIGGLAVGEPQDVMLQMLDTTLPVLPG
CCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEECCEECCCHHHHHHHHHHHHCCCCCC
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGKVNIRNARHAEDMRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCHHHCCC
LDDQSNCPASRDYSRAYLHHLVRSNEALGGMLLSWNNLAYYQELMQGIRKAIAEGRFADF
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
MAETMEEWARGDLDPV
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TENFQFTLKKTDAGARLGEISMPRGTIRTPAFMPVGTVGTVKAMYLDQVRETGADIILG
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEC
NTYHLMLRPSAERVARLGGLHKLIRWEHPILTDSGGFQVMSLSGLRKLDEQGVTFKSHVD
CEEEEEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCC
GSLHHMSPERSIEIQGLLGSDIQMQLDECVALPAEPREIERAMEMSLRWAERCRVAFGEQ
CCHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PGKAMFGIVQGGDIPALRVRSAQALSELDLKGYAIGGLAVGEPQDVMLQMLDTTLPVLPG
CCCEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEECCEECCCHHHHHHHHHHHHCCCCCC
EKPRYLMGVGTPDDILKSVARGIDMFDCVMPTRSGRHGLAFTRRGKVNIRNARHAEDMRP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEECCCEEECCCCCHHHCCC
LDDQSNCPASRDYSRAYLHHLVRSNEALGGMLLSWNNLAYYQELMQGIRKAIAEGRFADF
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHH
MAETMEEWARGDLDPV
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]