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The map label for this gene is nfdA [H]

Identifier: 209549266

GI number: 209549266

Start: 1697903

End: 1699885

Strand: Direct

Name: nfdA [H]

Synonym: Rleg2_1668

Alternate gene names: 209549266

Gene position: 1697903-1699885 (Clockwise)

Preceding gene: 209549265

Following gene: 209549267

Centisome position: 37.42

GC content: 61.57

Gene sequence:

>1983_bases
ATGACGACGCGTCGATCTTTTCTGGGAGCAGCATCGAGCCTGGCGTTCTCGAACCTCTTTTCCCCGGCGAAGGCCGCCGA
TCCCAACCAGACCGGAGTAGACACCATGCATCCCGACCTGATCCTTCACAACGGCCGCGTCACCACGCTTGACCGGTCCA
ATCCGAATGCAACGGCTATCGCCGTTAAGGACGGTCTCTTTGTCGAAGTCGGATCCGACAGCGAGATCATGGCGCTGGCT
GGGTCCAGCACCAGGGTCGTCGACCTCAAGGGTAAGCGGGTCTTGCCCGGGCTCATCGACAACCACACCCACGTGGTCCG
CGGCGGCCTGAACTACAACATGGAGCTGCGTTGGGACGGCGTCCGGTCGCTCGCCGATGCGATGGACATGCTAAAGCGCC
AGGTGGCGGTCACGCCCGCGCCGCAGTGGGTGCGCGTCGTCGGCGGGTTCAGCGAACATCAGTTTGCGGAAAAGCGCCTC
CCGACGATTGAGGAAATCAATGCTGTCGCGCCCGACACGCCGGTGTTCCTTCTGCACCTCTACGATCGCGCACTGCTCAA
CGGGGCGGCTTTGCGTGCAGTCGGATACACCCGTGACACGCCAAACCCGCCCGGCGGAGAGATCACCCGCGACGCCAATG
GCAATCCCACAGGCATGCTGCTGGCCAAGCCGAATGCAGGGGTTCTGTACTCGACACTCGCCAAGGGACCGAAACTTCCT
TTGGACTACCAGGTCAACTCGACCCGCCACTTCATGCGCGAGCTCAACCGCCTGGGCGTGACGGGCGTCATCGATGCCGG
CGGCGGCTTCCAGAACTATCCTGATGACTACGAAGTCATCCAGAAGCTCTCCGATGCGAACCAGATGACCGTTCGCCTTG
CCTACAATCTCTTTACCCAGAAGCCCAAGGAAGAGAAACAGGACTTCCTGAAGTGGACGCAGTCGGTCAAATACAAGCAG
GGCAACGACTACTTCCGCCACAACGGTGCCGGCGAGATGCTTGTCTTCTCCGCCGCAGATTTCGAGGATTTCCGCCAGCC
TCGTCCGGAGATGGCTCCGGAAATGGAAGGCGAGCTGGAGGAGGTCGTCCGTGTTCTGGCTGAAAACCGCTGGCCCTGGC
GTCTGCACGCCACCTATGACGAAACGATTTCCCGAGCCCTCGACGTGTTTGAGAAGGTCGACAAGGATATCCCGCTAGAA
GGTCTGAACTGGTTCTTCGATCACGCCGAAACGATCTCCGAACGTTCGATCGACCGGATCGCGGCGCTTGGCGGCGGCAT
CGCCACCCAGCATCGCATGGCCTATCAGGGGGAATACTTCGTCGAACGCTACGGTCACGGTGTAGCCGAGGCGACGCCGC
CGATCCGCCGCATGCTCGAAAAGGGCGTGAATGTCTCGGCAGGCACCGACGCCACCCGCGTTGCCTCTTACAACCCTTGG
GTTTCGCTCTCCTGGATGGTCACCGGCAAGACGGTCGGCGGCATGCAGCTCTATCCGCGCGCCAACTGCCTCGATCGCGA
GACGGCGTTGCGCATGTGGACCGAGAAGGTCACATGGTTCTCCAATGAGGAGGGCAAGAAGGGCCGTATCGAGAAGGGCC
AGTTCGCCGATCTGGTGGTGCCGGACAAGGACTTCTTCTCCTGCGCGGAAGACGAGATCTCCTTCCTCACTTCGGAACTG
ACCATGGTCGGCGGCAAGATTGTCTATGGGGCAGGCGACTTCAAGACGCTCGACGAGAACGACGTGCCGCCGGCGATGCC
CGACTGGTCTCCCGTCCGCAGATTCGGCGGATACGCGGCCTGGGGCGAACCGGAAGGCGCAGGCTCGCGCTCTTTGCGCC
GTACGGTGATTTCCACATGCGGATGTGCCAGTGACTGCGGCGTGCATGGCCATGACCATGCCGGACCCTGGACATCCAAA
CTTCCGATCGCCGACCTAAAGGGATTCTTCGGCGCTCTCGGGTGCTCCTGCTGGGCGGTATAA

Upstream 100 bases:

>100_bases
TCCATCAAATCAAGCCGCACATGTTCGGTCATTTGCCGAAGGGCGGCGACAGCATTGTCGGTGTTCATCAGGCGGAGGCG
AGGATGGAGGATCATAGCTG

Downstream 100 bases:

>100_bases
CTCAGCTTTGCGCTCAGGCGACACGTCGTCGCCCGAGCGCCTTTGTCGAGAATTGCAAAAACCGCAATTAAGTCAAAATG
ATTGCTGCAATTGTTGTTAA

Product: amidohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MTTRRSFLGAASSLAFSNLFSPAKAADPNQTGVDTMHPDLILHNGRVTTLDRSNPNATAIAVKDGLFVEVGSDSEIMALA
GSSTRVVDLKGKRVLPGLIDNHTHVVRGGLNYNMELRWDGVRSLADAMDMLKRQVAVTPAPQWVRVVGGFSEHQFAEKRL
PTIEEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPNPPGGEITRDANGNPTGMLLAKPNAGVLYSTLAKGPKLP
LDYQVNSTRHFMRELNRLGVTGVIDAGGGFQNYPDDYEVIQKLSDANQMTVRLAYNLFTQKPKEEKQDFLKWTQSVKYKQ
GNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEMEGELEEVVRVLAENRWPWRLHATYDETISRALDVFEKVDKDIPLE
GLNWFFDHAETISERSIDRIAALGGGIATQHRMAYQGEYFVERYGHGVAEATPPIRRMLEKGVNVSAGTDATRVASYNPW
VSLSWMVTGKTVGGMQLYPRANCLDRETALRMWTEKVTWFSNEEGKKGRIEKGQFADLVVPDKDFFSCAEDEISFLTSEL
TMVGGKIVYGAGDFKTLDENDVPPAMPDWSPVRRFGGYAAWGEPEGAGSRSLRRTVISTCGCASDCGVHGHDHAGPWTSK
LPIADLKGFFGALGCSCWAV

Sequences:

>Translated_660_residues
MTTRRSFLGAASSLAFSNLFSPAKAADPNQTGVDTMHPDLILHNGRVTTLDRSNPNATAIAVKDGLFVEVGSDSEIMALA
GSSTRVVDLKGKRVLPGLIDNHTHVVRGGLNYNMELRWDGVRSLADAMDMLKRQVAVTPAPQWVRVVGGFSEHQFAEKRL
PTIEEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPNPPGGEITRDANGNPTGMLLAKPNAGVLYSTLAKGPKLP
LDYQVNSTRHFMRELNRLGVTGVIDAGGGFQNYPDDYEVIQKLSDANQMTVRLAYNLFTQKPKEEKQDFLKWTQSVKYKQ
GNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEMEGELEEVVRVLAENRWPWRLHATYDETISRALDVFEKVDKDIPLE
GLNWFFDHAETISERSIDRIAALGGGIATQHRMAYQGEYFVERYGHGVAEATPPIRRMLEKGVNVSAGTDATRVASYNPW
VSLSWMVTGKTVGGMQLYPRANCLDRETALRMWTEKVTWFSNEEGKKGRIEKGQFADLVVPDKDFFSCAEDEISFLTSEL
TMVGGKIVYGAGDFKTLDENDVPPAMPDWSPVRRFGGYAAWGEPEGAGSRSLRRTVISTCGCASDCGVHGHDHAGPWTSK
LPIADLKGFFGALGCSCWAV
>Mature_659_residues
TTRRSFLGAASSLAFSNLFSPAKAADPNQTGVDTMHPDLILHNGRVTTLDRSNPNATAIAVKDGLFVEVGSDSEIMALAG
SSTRVVDLKGKRVLPGLIDNHTHVVRGGLNYNMELRWDGVRSLADAMDMLKRQVAVTPAPQWVRVVGGFSEHQFAEKRLP
TIEEINAVAPDTPVFLLHLYDRALLNGAALRAVGYTRDTPNPPGGEITRDANGNPTGMLLAKPNAGVLYSTLAKGPKLPL
DYQVNSTRHFMRELNRLGVTGVIDAGGGFQNYPDDYEVIQKLSDANQMTVRLAYNLFTQKPKEEKQDFLKWTQSVKYKQG
NDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEMEGELEEVVRVLAENRWPWRLHATYDETISRALDVFEKVDKDIPLEG
LNWFFDHAETISERSIDRIAALGGGIATQHRMAYQGEYFVERYGHGVAEATPPIRRMLEKGVNVSAGTDATRVASYNPWV
SLSWMVTGKTVGGMQLYPRANCLDRETALRMWTEKVTWFSNEEGKKGRIEKGQFADLVVPDKDFFSCAEDEISFLTSELT
MVGGKIVYGAGDFKTLDENDVPPAMPDWSPVRRFGGYAAWGEPEGAGSRSLRRTVISTCGCASDCGVHGHDHAGPWTSKL
PIADLKGFFGALGCSCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 72894; Mature: 72763

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRRSFLGAASSLAFSNLFSPAKAADPNQTGVDTMHPDLILHNGRVTTLDRSNPNATAI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCEEEE
AVKDGLFVEVGSDSEIMALAGSSTRVVDLKGKRVLPGLIDNHTHVVRGGLNYNMELRWDG
EEECCEEEEECCCCCEEEEECCCEEEEEECCCEECCHHHCCCCEEEECCCCCCEEEEECH
VRSLADAMDMLKRQVAVTPAPQWVRVVGGFSEHQFAEKRLPTIEEINAVAPDTPVFLLHL
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCEEHHHH
YDRALLNGAALRAVGYTRDTPNPPGGEITRDANGNPTGMLLAKPNAGVLYSTLAKGPKLP
HHHHHHCCHHHEEECCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHCCCCCC
LDYQVNSTRHFMRELNRLGVTGVIDAGGGFQNYPDDYEVIQKLSDANQMTVRLAYNLFTQ
EEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHC
KPKEEKQDFLKWTQSVKYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEMEGELE
CCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCHHHHHCCCCCCCCCCCHHHH
EVVRVLAENRWPWRLHATYDETISRALDVFEKVDKDIPLEGLNWFFDHAETISERSIDRI
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AALGGGIATQHRMAYQGEYFVERYGHGVAEATPPIRRMLEKGVNVSAGTDATRVASYNPW
HHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCC
VSLSWMVTGKTVGGMQLYPRANCLDRETALRMWTEKVTWFSNEEGKKGRIEKGQFADLVV
EEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEC
PDKDFFSCAEDEISFLTSELTMVGGKIVYGAGDFKTLDENDVPPAMPDWSPVRRFGGYAA
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
WGEPEGAGSRSLRRTVISTCGCASDCGVHGHDHAGPWTSKLPIADLKGFFGALGCSCWAV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure 
TTRRSFLGAASSLAFSNLFSPAKAADPNQTGVDTMHPDLILHNGRVTTLDRSNPNATAI
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCEEEEEECCCCCEEEE
AVKDGLFVEVGSDSEIMALAGSSTRVVDLKGKRVLPGLIDNHTHVVRGGLNYNMELRWDG
EEECCEEEEECCCCCEEEEECCCEEEEEECCCEECCHHHCCCCEEEECCCCCCEEEEECH
VRSLADAMDMLKRQVAVTPAPQWVRVVGGFSEHQFAEKRLPTIEEINAVAPDTPVFLLHL
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCEEHHHH
YDRALLNGAALRAVGYTRDTPNPPGGEITRDANGNPTGMLLAKPNAGVLYSTLAKGPKLP
HHHHHHCCHHHEEECCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHCCCCCC
LDYQVNSTRHFMRELNRLGVTGVIDAGGGFQNYPDDYEVIQKLSDANQMTVRLAYNLFTQ
EEEEECHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHC
KPKEEKQDFLKWTQSVKYKQGNDYFRHNGAGEMLVFSAADFEDFRQPRPEMAPEMEGELE
CCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCEEEEECCCHHHHHCCCCCCCCCCCHHHH
EVVRVLAENRWPWRLHATYDETISRALDVFEKVDKDIPLEGLNWFFDHAETISERSIDRI
HHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
AALGGGIATQHRMAYQGEYFVERYGHGVAEATPPIRRMLEKGVNVSAGTDATRVASYNPW
HHHCCCHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCC
VSLSWMVTGKTVGGMQLYPRANCLDRETALRMWTEKVTWFSNEEGKKGRIEKGQFADLVV
EEEEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEC
PDKDFFSCAEDEISFLTSELTMVGGKIVYGAGDFKTLDENDVPPAMPDWSPVRRFGGYAA
CCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCC
WGEPEGAGSRSLRRTVISTCGCASDCGVHGHDHAGPWTSKLPIADLKGFFGALGCSCWAV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA