Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 209549247

GI number: 209549247

Start: 1677910

End: 1678767

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_1648

Alternate gene names: 209549247

Gene position: 1677910-1678767 (Clockwise)

Preceding gene: 209549245

Following gene: 209549248

Centisome position: 36.98

GC content: 64.34

Gene sequence:

>858_bases
ATGCGGCCGCCATTGGAGTCCTTGCGAATTCTGGAAGCGTGTGTTTCTGCCGGCAGCTTTGCCCGCGCGGCCGAGCGTTT
ATGCCTCACTCCAGCGGCAGTCAGCCTGCGTATCCGTACGTTGGAGACCGAACTGGGTCAGCCGCTGTTCCAGCGTGCTG
GACGCCGCGTTGTCCCGACGGCTGCTGCCGCTGTTCTGGCGGGCCGAATTCGGGCGGCATTGGATGGCATTGCCGGCGCG
CTTGATGAGTTCCAGGCGGCCATGCCACCGCTTCGGCTGACGGCGCCTCCGACATTCGCCTCGCGCTGGCTCGCACCGCG
GCTGTCCCGCTATCCCTCAGTGGGAACATCCCCGATCGAGGTCGACGTCTCCGCCGATATTCGCGATCCCAGCGCATTTG
ACGTTGCCATACGGACGGGGCGTGGCGGATGGGAAGGGCTTGATGAATATCGGCTCGCTCCGGTTGAGGCAACACCGATG
CTGGCGCCCTCACTGCTTGGAACGAGAACCCTGAACGGGCCGGAGGGGCTGGCCGATTTCGAGCTTTTGCCGCATCCGGA
TTGGGATGCCTGGTTCAGAGGCGCTCAATGCGAGACGCCGCCGGCTCTGCGGTTTGCGATGGTCGATTATCCCACGCACG
AATTGGACGCCAGTGCGGCGGTAGCAGGTGTCGGCGTGGCGCTGTTGTCGCAGTCCTTGTTTCGACCGCTTTTGGAGAAG
GGATTGCTAATCGCACCCTTCCCATATGTGCTGACCGGGCCGGCCTGGCATTTCGCGTTGATGCGCGCCGACGACTCCCG
TCCCGCGCCCAGGCAGCTCTGCGCATGGCTTTGCGAGCGGGCTCATGAGCCAGCCTGA

Upstream 100 bases:

>100_bases
CTCCTATTGTTGACCTCAACCCTAGCTGCAAGCGCTCTGTCCCCAAAACGCGCTTTGCTTGTGAGCATCACAAGAAAAAC
TCATCAAAAGCGAAGAGATC

Downstream 100 bases:

>100_bases
TAGGGAACAACCTGCGACGGGCCAAATCTGCTGCAGGCACACCCGATCAATTTGCCTTCCCGGAAGCAATCGAGCCCCTA
TATCGCCCTCGGACAGTCGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MRPPLESLRILEACVSAGSFARAAERLCLTPAAVSLRIRTLETELGQPLFQRAGRRVVPTAAAAVLAGRIRAALDGIAGA
LDEFQAAMPPLRLTAPPTFASRWLAPRLSRYPSVGTSPIEVDVSADIRDPSAFDVAIRTGRGGWEGLDEYRLAPVEATPM
LAPSLLGTRTLNGPEGLADFELLPHPDWDAWFRGAQCETPPALRFAMVDYPTHELDASAAVAGVGVALLSQSLFRPLLEK
GLLIAPFPYVLTGPAWHFALMRADDSRPAPRQLCAWLCERAHEPA

Sequences:

>Translated_285_residues
MRPPLESLRILEACVSAGSFARAAERLCLTPAAVSLRIRTLETELGQPLFQRAGRRVVPTAAAAVLAGRIRAALDGIAGA
LDEFQAAMPPLRLTAPPTFASRWLAPRLSRYPSVGTSPIEVDVSADIRDPSAFDVAIRTGRGGWEGLDEYRLAPVEATPM
LAPSLLGTRTLNGPEGLADFELLPHPDWDAWFRGAQCETPPALRFAMVDYPTHELDASAAVAGVGVALLSQSLFRPLLEK
GLLIAPFPYVLTGPAWHFALMRADDSRPAPRQLCAWLCERAHEPA
>Mature_285_residues
MRPPLESLRILEACVSAGSFARAAERLCLTPAAVSLRIRTLETELGQPLFQRAGRRVVPTAAAAVLAGRIRAALDGIAGA
LDEFQAAMPPLRLTAPPTFASRWLAPRLSRYPSVGTSPIEVDVSADIRDPSAFDVAIRTGRGGWEGLDEYRLAPVEATPM
LAPSLLGTRTLNGPEGLADFELLPHPDWDAWFRGAQCETPPALRFAMVDYPTHELDASAAVAGVGVALLSQSLFRPLLEK
GLLIAPFPYVLTGPAWHFALMRADDSRPAPRQLCAWLCERAHEPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=262, Percent_Identity=33.969465648855, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=32.5581395348837, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788706, Length=273, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30746; Mature: 30746

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPLESLRILEACVSAGSFARAAERLCLTPAAVSLRIRTLETELGQPLFQRAGRRVVPT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEEHHHHHHCCHHHHHCCCEECHH
AAAAVLAGRIRAALDGIAGALDEFQAAMPPLRLTAPPTFASRWLAPRLSRYPSVGTSPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCEE
VDVSADIRDPSAFDVAIRTGRGGWEGLDEYRLAPVEATPMLAPSLLGTRTLNGPEGLADF
EEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
ELLPHPDWDAWFRGAQCETPPALRFAMVDYPTHELDASAAVAGVGVALLSQSLFRPLLEK
CCCCCCCHHHHHCCCCCCCCHHEEEEEECCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHC
GLLIAPFPYVLTGPAWHFALMRADDSRPAPRQLCAWLCERAHEPA
CCEEECCCHHHCCCHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRPPLESLRILEACVSAGSFARAAERLCLTPAAVSLRIRTLETELGQPLFQRAGRRVVPT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHEEEEEHHHHHHCCHHHHHCCCEECHH
AAAAVLAGRIRAALDGIAGALDEFQAAMPPLRLTAPPTFASRWLAPRLSRYPSVGTSPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCEE
VDVSADIRDPSAFDVAIRTGRGGWEGLDEYRLAPVEATPMLAPSLLGTRTLNGPEGLADF
EEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
ELLPHPDWDAWFRGAQCETPPALRFAMVDYPTHELDASAAVAGVGVALLSQSLFRPLLEK
CCCCCCCHHHHHCCCCCCCCHHEEEEEECCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHC
GLLIAPFPYVLTGPAWHFALMRADDSRPAPRQLCAWLCERAHEPA
CCEEECCCHHHCCCHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]