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The map label for this gene is lipA [H]

Identifier: 209549210

GI number: 209549210

Start: 1637312

End: 1638283

Strand: Direct

Name: lipA [H]

Synonym: Rleg2_1611

Alternate gene names: 209549210

Gene position: 1637312-1638283 (Clockwise)

Preceding gene: 209549209

Following gene: 209549211

Centisome position: 36.08

GC content: 62.86

Gene sequence:

>972_bases
ATGGTGACCATTCTCGATACGATCAATCCCGACGCCAAGCGCGTGCGTCATCCCGAGAAGGCGCATAGGCCCGATACCGA
GGTCATGCGCAAGCCGGACTGGATCCGCGTCAAGGCGCCGACCTCCAAAGGTTACGCCGAGACGCGCGCGATCGTGAAGG
AGCACAAGCTCGTCACCGTCTGCGAGGAGGCCGGCTGCCCGAATATCGGCGAGTGCTGGGACAAGAAGCACGCCACCTTC
ATGATCATGGGTGAGATCTGTACGCGCGCCTGCGCCTTCTGCAATGTCGCCACCGGCAAGCCGAACGCGCTCGATATGGC
CGAGCCGGAAAACGTCGCCAAGGCGGTCAAGGAGATGGGCCTCAGCCACGTCGTCATCACTTCGGTCGACCGCGACGACC
TGGAGGACGGCGGTGCTGAACACTTCGAAAAGGTGATCTGGGCGATCCGCGCGGCATCGCCGGCAACGACGATCGAAATC
CTGACGCCCGACTTCCTGAAGAAGCCGGGCGCTTTGGAGCGCGTCGTCGCCGCCAAACCCGACGTTTTCAACCACAACAT
GGAAACGGTCCCCGGCAACTATCTGACGGTTCGCCCCGGCGCCCGCTATTTCCATTCCATCCGGCTGCTGCAGCGGGTGA
AGGAACTCGATCCCACCATGTTCACCAAATCGGGCATCATGGTCGGCCTCGGCGAGGAGCGCAACGAGGTGCTGCAGCTG
ATGGACGATCTGCGCACCGCCGATGTCGATTTCCTGACCATCGGCCAGTACCTGCAGCCGACCCGCAAGCACCACAAGGT
CGAAAGCTTCGTCACGCCGGACGAGTTCAAGTCCTACGAAACGGTCGCCTACAGCAAGGGCTTCCTGATGGTCGCCTCCA
GCCCGCTGACCCGCTCCTCCCACCATGCCGGCGACGACTTCGCCCGGCTGAGAGCGGCGCGCGAAAAGAAGCTGCTGATG
GCGGCGGAGTAA

Upstream 100 bases:

>100_bases
TTTCCTCGGCGCCTGCCTGCTGATATTCCTTGCACGGCTGGTGCGGCGCTAGATCCAAGAGATGATGCCGCTTTAAGGGC
GGCGGAAGGCTGATACTGCA

Downstream 100 bases:

>100_bases
GATCCGGCGCTTGCTTATAGAAGCGGCTCCGGCAATCTTTCACTGCGATCAGACAGCTGGTAGACAATGCCTGCCAACCT
CTCTTTCGTCATGCTAGGAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MVTILDTINPDAKRVRHPEKAHRPDTEVMRKPDWIRVKAPTSKGYAETRAIVKEHKLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGKPNALDMAEPENVAKAVKEMGLSHVVITSVDRDDLEDGGAEHFEKVIWAIRAASPATTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVESFVTPDEFKSYETVAYSKGFLMVASSPLTRSSHHAGDDFARLRAAREKKLLM
AAE

Sequences:

>Translated_323_residues
MVTILDTINPDAKRVRHPEKAHRPDTEVMRKPDWIRVKAPTSKGYAETRAIVKEHKLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGKPNALDMAEPENVAKAVKEMGLSHVVITSVDRDDLEDGGAEHFEKVIWAIRAASPATTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVESFVTPDEFKSYETVAYSKGFLMVASSPLTRSSHHAGDDFARLRAAREKKLLM
AAE
>Mature_323_residues
MVTILDTINPDAKRVRHPEKAHRPDTEVMRKPDWIRVKAPTSKGYAETRAIVKEHKLVTVCEEAGCPNIGECWDKKHATF
MIMGEICTRACAFCNVATGKPNALDMAEPENVAKAVKEMGLSHVVITSVDRDDLEDGGAEHFEKVIWAIRAASPATTIEI
LTPDFLKKPGALERVVAAKPDVFNHNMETVPGNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
MDDLRTADVDFLTIGQYLQPTRKHHKVESFVTPDEFKSYETVAYSKGFLMVASSPLTRSSHHAGDDFARLRAAREKKLLM
AAE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=301, Percent_Identity=46.1794019933555, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=46.8253968253968, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=50.7194244604317, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=44.9612403100775, Blast_Score=236, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=42.9054054054054, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI221513272, Length=311, Percent_Identity=43.0868167202572, Blast_Score=255, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36142; Mature: 36142

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTILDTINPDAKRVRHPEKAHRPDTEVMRKPDWIRVKAPTSKGYAETRAIVKEHKLVTV
CEEEECCCCCCHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHEEE
CEEAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGKPNALDMAEPENVAKAVKEMG
HHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLEDGGAEHFEKVIWAIRAASPATTIEILTPDFLKKPGALERVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNMETVPGNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHH
MDDLRTADVDFLTIGQYLQPTRKHHKVESFVTPDEFKSYETVAYSKGFLMVASSPLTRSS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHEECCCEEEEECCCCCCCC
HHAGDDFARLRAAREKKLLMAAE
CCCCHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MVTILDTINPDAKRVRHPEKAHRPDTEVMRKPDWIRVKAPTSKGYAETRAIVKEHKLVTV
CEEEECCCCCCHHHHCCCHHHCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHEEE
CEEAGCPNIGECWDKKHATFMIMGEICTRACAFCNVATGKPNALDMAEPENVAKAVKEMG
HHHCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCC
LSHVVITSVDRDDLEDGGAEHFEKVIWAIRAASPATTIEILTPDFLKKPGALERVVAAKP
CCEEEEEECCHHHCCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHCCCHHHHHHHCCC
DVFNHNMETVPGNYLTVRPGARYFHSIRLLQRVKELDPTMFTKSGIMVGLGEERNEVLQL
CHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCHHHHHHH
MDDLRTADVDFLTIGQYLQPTRKHHKVESFVTPDEFKSYETVAYSKGFLMVASSPLTRSS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHEECCCEEEEECCCCCCCC
HHAGDDFARLRAAREKKLLMAAE
CCCCHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA