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The map label for this gene is cspA [H]

Identifier: 209549149

GI number: 209549149

Start: 1576427

End: 1577005

Strand: Direct

Name: cspA [H]

Synonym: Rleg2_1550

Alternate gene names: 209549149

Gene position: 1576427-1577005 (Clockwise)

Preceding gene: 209549146

Following gene: 209549150

Centisome position: 34.74

GC content: 62.0

Gene sequence:

>579_bases
ATGGCTGACAGGATTTCATCGAACGAATACTCCGACCTTGACGAGCTATCGGCAGAGGCGGTCGATCTTGTTGAAATCAC
CGGCGTCGTCAAGTGGTTCGACGTCGCTAAGGGTTTCGGCTTCATCGTGCCCGACAACGGCATGCAGGATGTTCTCCTGC
ACGTGACCTGCCTGCGCCGCGACGGTTACCAGACGATCCTCGAGGGAACGCGCATCGTCGCCCTCATCCAGCGTCGCGAA
CGTGGCTACCAGGCTTTCAAGATCCTTTCGATGGATCAGTCGACTGCGGTTCATCCCTCGCAACTGCCGCCGGTGCGCAC
ACATGTGCAGGTCACCGCGACGAGCGGCCTTGAGCGTGCGCTGGTCAAATGGTTCAACCGCACCAAGGGCTTCGGCTTCC
TGACGCGCGGCGAGGGCACGGAAGACATCTTCGTGCACATGGAAACGCTCCGCCGCTTCGGCCTGACGGAACTGCGCCCC
GGCCAGGTTGTGCTCGTCCGCTTCGGCGACGGCGAAAAGGGCCTGATGGCCGCGGAAATCCATCCCGACATTCCGAGCCC
GGCCAGCCGGTCGCATTGA

Upstream 100 bases:

>100_bases
AAAACTAGGACTTGCGCTTATCCGTTACCAAGATGTTAATCTTGATTTCAAGAATCAGTTAACCGTAACAGTGTGACGCG
TATTCGAGGGGCTGGCGACT

Downstream 100 bases:

>100_bases
TATGCGTGACATCGTCCTTCTTCATGCGATCAAAAGCGCCATCCTGGCGCTTTTTTTCATGGTTGCGCTGCCGGCTTTCG
CCGAGGAGCAGATCCACTTC

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-A; 7.4 kDa cold shock protein; CS7.4 [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MADRISSNEYSDLDELSAEAVDLVEITGVVKWFDVAKGFGFIVPDNGMQDVLLHVTCLRRDGYQTILEGTRIVALIQRRE
RGYQAFKILSMDQSTAVHPSQLPPVRTHVQVTATSGLERALVKWFNRTKGFGFLTRGEGTEDIFVHMETLRRFGLTELRP
GQVVLVRFGDGEKGLMAAEIHPDIPSPASRSH

Sequences:

>Translated_192_residues
MADRISSNEYSDLDELSAEAVDLVEITGVVKWFDVAKGFGFIVPDNGMQDVLLHVTCLRRDGYQTILEGTRIVALIQRRE
RGYQAFKILSMDQSTAVHPSQLPPVRTHVQVTATSGLERALVKWFNRTKGFGFLTRGEGTEDIFVHMETLRRFGLTELRP
GQVVLVRFGDGEKGLMAAEIHPDIPSPASRSH
>Mature_191_residues
ADRISSNEYSDLDELSAEAVDLVEITGVVKWFDVAKGFGFIVPDNGMQDVLLHVTCLRRDGYQTILEGTRIVALIQRRER
GYQAFKILSMDQSTAVHPSQLPPVRTHVQVTATSGLERALVKWFNRTKGFGFLTRGEGTEDIFVHMETLRRFGLTELRPG
QVVLVRFGDGEKGLMAAEIHPDIPSPASRSH

Specific function: Binds to and stimulates the transcription of the CCAAT- containing, cold-shock-inducible promoters of the H-NS and GyrA proteins. Binds also to the inverted repeat 5'-ATTGG-3' [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=67, Percent_Identity=40.2985074626866, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1787839, Length=68, Percent_Identity=38.2352941176471, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=36.231884057971, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI2367114, Length=67, Percent_Identity=37.3134328358209, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 21454; Mature: 21323

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRISSNEYSDLDELSAEAVDLVEITGVVKWFDVAKGFGFIVPDNGMQDVLLHVTCLRR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHC
DGYQTILEGTRIVALIQRRERGYQAFKILSMDQSTAVHPSQLPPVRTHVQVTATSGLERA
CCHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHH
LVKWFNRTKGFGFLTRGEGTEDIFVHMETLRRFGLTELRPGQVVLVRFGDGEKGLMAAEI
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
HPDIPSPASRSH
CCCCCCCCCCCC
>Mature Secondary Structure 
ADRISSNEYSDLDELSAEAVDLVEITGVVKWFDVAKGFGFIVPDNGMQDVLLHVTCLRR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHC
DGYQTILEGTRIVALIQRRERGYQAFKILSMDQSTAVHPSQLPPVRTHVQVTATSGLERA
CCHHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCHHHH
LVKWFNRTKGFGFLTRGEGTEDIFVHMETLRRFGLTELRPGQVVLVRFGDGEKGLMAAEI
HHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEEEE
HPDIPSPASRSH
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]