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The map label for this gene is ilvA [H]

Identifier: 209549024

GI number: 209549024

Start: 1445486

End: 1446736

Strand: Direct

Name: ilvA [H]

Synonym: Rleg2_1421

Alternate gene names: 209549024

Gene position: 1445486-1446736 (Clockwise)

Preceding gene: 209549022

Following gene: 209549025

Centisome position: 31.85

GC content: 62.27

Gene sequence:

>1251_bases
GTGACGAGACTTGATGTCGAAAGTGCCGAAGAGGCAATGCGCAGCCTTTTTCCGGCAACGCCGCTGCAGCTCAACGATCA
TCTCTCGGCGCGTTACGGGGCCGATATCTGGCTGAAGCGCGAGGATCTATCGCCGGTGCGCTCCTACAAGATCCGCGGTG
CCTTCAACTTCTTCCGCAAGGCGATCGGGCAGGGTGCTTCCGGCAAGACCTTCGTCTGCGCCTCGGCCGGCAATCACGCC
CAGGGTTTCGCCTTCGTCTGCCGCCATTTCGGCGTGCCGGGCGTCGTCTTCATGCCGGTGACGACGCCGCAACAGAAGAT
CGACAAGACCCGCATGTTCGGCGCCGAATTCATCACCATCGGGCTGTTCGGGGACTTCTTCGACCAGTGCTACCAGGCCG
CGCGCGACCATGTCGAGGCGGTCGGCGGCGTCATGGTGCCGCCCTTCGACCATGCCGATATTATCGAAGGGCAGGCGACG
GTCGCTGCCGAGATCATGCAGCAGCTGCCTGAGGGAACGGTGCCTGACATGGTCGTCCTGCCGGTCGGCGGCGGCGGGCT
TGCCGCCGGCATCACCGGTTATCTCGACGGCAAAGTGCCGAAATCGGCCTTCGTCTTTACCGAGCCCGCCGGCGCGCCGA
GCCTGAGGCGCAGCATCGAGGCAGGCAAGGTGACGACGCTTGCCAAGGTCGACAACTTCGTCGACGGCGCTGCCGTCGCC
CGCATCGGCGACTTGAATTTCGCCGCTCTTCGCGACTTCCCCGCAAGCCAAGTGCAGCTGATGCCGGAGAACGCCATCTG
CGTCACCATCCAGGAGATGCTGAATGTCGAAGGCGTCGTGCTGGAGCCGGCAGGCGCTCTGTCGCTGACCGCGATCGCTG
CGATGGACGCCCAGGCGATCCGCGGCAAGACCATCGTCGCCATCGTTTCCGGCGGCAATTTCGATTTCGAGCGCCTGCCT
GACGTAAAGGAAAGAGCCATGCGTTACGCAGGGCTGAAGAAATACTTCATCCTCAGGCTCGCCCAGCGCCCCGGCGCGCT
TCGAGATTTCCTCAATCTGCTCGGCCCTGACGACGATATCGCCCGCTTCGAATATCTGAAGAAATCGGCGCGCAATTTCG
GCTCGATCCTGATCGGCATCGAAACCAAGGCGCCGGAGAATTTCGCCCGGCTGATCGGAAATTTCGAAGCCGCCGGCATG
GGTTACGAGGATATCACCGAAAACGAGATCCTCGCCAACCTGATCATTTGA

Upstream 100 bases:

>100_bases
AAACAGGCCTGGGCCGAACCGTAAACGGCTGAAGCGGCCTTTCCTGTGCCGGCCGGCCTGTGCGATAGCGGCGGTATCAT
TACGAGAATGGATCACGCCC

Downstream 100 bases:

>100_bases
CTTGGCGACAGCAATGCCATCGTGCTAAAGGCGGATCATGGCTTCGTTCCTTTCAAATCTCTTTTCGATGTTCTCCGGCG
GCGGCAAACCGGCGTCCGAA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTRLDVESAEEAMRSLFPATPLQLNDHLSARYGADIWLKREDLSPVRSYKIRGAFNFFRKAIGQGASGKTFVCASAGNHA
QGFAFVCRHFGVPGVVFMPVTTPQQKIDKTRMFGAEFITIGLFGDFFDQCYQAARDHVEAVGGVMVPPFDHADIIEGQAT
VAAEIMQQLPEGTVPDMVVLPVGGGGLAAGITGYLDGKVPKSAFVFTEPAGAPSLRRSIEAGKVTTLAKVDNFVDGAAVA
RIGDLNFAALRDFPASQVQLMPENAICVTIQEMLNVEGVVLEPAGALSLTAIAAMDAQAIRGKTIVAIVSGGNFDFERLP
DVKERAMRYAGLKKYFILRLAQRPGALRDFLNLLGPDDDIARFEYLKKSARNFGSILIGIETKAPENFARLIGNFEAAGM
GYEDITENEILANLII

Sequences:

>Translated_416_residues
MTRLDVESAEEAMRSLFPATPLQLNDHLSARYGADIWLKREDLSPVRSYKIRGAFNFFRKAIGQGASGKTFVCASAGNHA
QGFAFVCRHFGVPGVVFMPVTTPQQKIDKTRMFGAEFITIGLFGDFFDQCYQAARDHVEAVGGVMVPPFDHADIIEGQAT
VAAEIMQQLPEGTVPDMVVLPVGGGGLAAGITGYLDGKVPKSAFVFTEPAGAPSLRRSIEAGKVTTLAKVDNFVDGAAVA
RIGDLNFAALRDFPASQVQLMPENAICVTIQEMLNVEGVVLEPAGALSLTAIAAMDAQAIRGKTIVAIVSGGNFDFERLP
DVKERAMRYAGLKKYFILRLAQRPGALRDFLNLLGPDDDIARFEYLKKSARNFGSILIGIETKAPENFARLIGNFEAAGM
GYEDITENEILANLII
>Mature_415_residues
TRLDVESAEEAMRSLFPATPLQLNDHLSARYGADIWLKREDLSPVRSYKIRGAFNFFRKAIGQGASGKTFVCASAGNHAQ
GFAFVCRHFGVPGVVFMPVTTPQQKIDKTRMFGAEFITIGLFGDFFDQCYQAARDHVEAVGGVMVPPFDHADIIEGQATV
AAEIMQQLPEGTVPDMVVLPVGGGGLAAGITGYLDGKVPKSAFVFTEPAGAPSLRRSIEAGKVTTLAKVDNFVDGAAVAR
IGDLNFAALRDFPASQVQLMPENAICVTIQEMLNVEGVVLEPAGALSLTAIAAMDAQAIRGKTIVAIVSGGNFDFERLPD
VKERAMRYAGLKKYFILRLAQRPGALRDFLNLLGPDDDIARFEYLKKSARNFGSILIGIETKAPENFARLIGNFEAAGMG
YEDITENEILANLII

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=318, Percent_Identity=27.6729559748428, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI33469958, Length=318, Percent_Identity=27.9874213836478, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI19923959, Length=326, Percent_Identity=28.2208588957055, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790207, Length=393, Percent_Identity=32.3155216284987, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789505, Length=319, Percent_Identity=31.3479623824451, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1789235, Length=211, Percent_Identity=28.436018957346, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71991565, Length=354, Percent_Identity=30.5084745762712, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537387, Length=400, Percent_Identity=28.25, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17508781, Length=288, Percent_Identity=27.0833333333333, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320930, Length=353, Percent_Identity=33.9943342776204, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322631, Length=315, Percent_Identity=28.5714285714286, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319788, Length=292, Percent_Identity=26.7123287671233, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355833, Length=353, Percent_Identity=32.2946175637394, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24645328, Length=259, Percent_Identity=32.8185328185328, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 44915; Mature: 44784

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLDVESAEEAMRSLFPATPLQLNDHLSARYGADIWLKREDLSPVRSYKIRGAFNFFRK
CCCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCCHHHHEEHHHHHHHHHH
AIGQGASGKTFVCASAGNHAQGFAFVCRHFGVPGVVFMPVTTPQQKIDKTRMFGAEFITI
HHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEE
GLFGDFFDQCYQAARDHVEAVGGVMVPPFDHADIIEGQATVAAEIMQQLPEGTVPDMVVL
HHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEE
PVGGGGLAAGITGYLDGKVPKSAFVFTEPAGAPSLRRSIEAGKVTTLAKVDNFVDGAAVA
EECCCCHHHCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHCCHHHH
RIGDLNFAALRDFPASQVQLMPENAICVTIQEMLNVEGVVLEPAGALSLTAIAAMDAQAI
EECCCCHHHHHCCCCHHEEECCCCCEEEEHHHHHCCCCEEECCCCCHHHHHHHHHHHHHH
RGKTIVAIVSGGNFDFERLPDVKERAMRYAGLKKYFILRLAQRPGALRDFLNLLGPDDDI
CCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
ARFEYLKKSARNFGSILIGIETKAPENFARLIGNFEAAGMGYEDITENEILANLII
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCHHCCCCHHHCCHHHHHHHHCC
>Mature Secondary Structure 
TRLDVESAEEAMRSLFPATPLQLNDHLSARYGADIWLKREDLSPVRSYKIRGAFNFFRK
CCCCHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEECCCCCHHHHEEHHHHHHHHHH
AIGQGASGKTFVCASAGNHAQGFAFVCRHFGVPGVVFMPVTTPQQKIDKTRMFGAEFITI
HHCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEE
GLFGDFFDQCYQAARDHVEAVGGVMVPPFDHADIIEGQATVAAEIMQQLPEGTVPDMVVL
HHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCEEEE
PVGGGGLAAGITGYLDGKVPKSAFVFTEPAGAPSLRRSIEAGKVTTLAKVDNFVDGAAVA
EECCCCHHHCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHCCHHHH
RIGDLNFAALRDFPASQVQLMPENAICVTIQEMLNVEGVVLEPAGALSLTAIAAMDAQAI
EECCCCHHHHHCCCCHHEEECCCCCEEEEHHHHHCCCCEEECCCCCHHHHHHHHHHHHHH
RGKTIVAIVSGGNFDFERLPDVKERAMRYAGLKKYFILRLAQRPGALRDFLNLLGPDDDI
CCCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHH
ARFEYLKKSARNFGSILIGIETKAPENFARLIGNFEAAGMGYEDITENEILANLII
HHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCHHCCCCHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]