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The map label for this gene is tuf2 [H]

Identifier: 209548933

GI number: 209548933

Start: 1360970

End: 1362145

Strand: Direct

Name: tuf2 [H]

Synonym: Rleg2_1330

Alternate gene names: 209548933

Gene position: 1360970-1362145 (Clockwise)

Preceding gene: 209548932

Following gene: 209548934

Centisome position: 29.99

GC content: 61.99

Gene sequence:

>1176_bases
ATGGCAAAGAGTAAGTTTGAGCGCAACAAGCCGCACGTCAACATTGGCACGATCGGCCACGTTGACCACGGCAAGACGTC
TCTGACGGCAGCGATCACGAAGTACTTCGGTGAGTTCAAGGCGTACGACCAGATCGACGCTGCTCCGGAAGAAAAGGCCC
GCGGCATCACCATTTCGACGGCGCACGTCGAGTATGAGACGCCGGCCCGCCACTACGCCCACGTCGACTGCCCCGGCCAC
GCCGACTACGTCAAGAACATGATCACCGGTGCTGCCCAGATGGACGGCGCGATCCTGGTGTGCTCGGCCGCTGACGGCCC
GATGCCGCAGACGCGCGAACACATCCTGCTGGCCCGCCAGGTCGGCGTTCCGGCGATCGTTGTGTTCCTGAACAAGGTCG
ACCAGGTTGACGACGCCGAGCTTCTCGAACTGGTCGAGCTCGAAGTTCGCGAACTGCTGTCGTCCTACGACTTCCCGGGC
GACGATATCCCGGTCGTCAAGGGTTCGGCTCTTGCTGCTCTTGAAGATTCTGACAAGAAGATCGGCGAAGACGCGATCCG
CGAGCTGATGGCTGCGGTTGACGCCTACATCCCGACGCCTGAGCGTCCGATCGACCAGCCGTTCCTGATGCCGATCGAAG
ACGTGTTCTCGATCTCCGGCCGCGGCACGGTTGTGACCGGCCGCGTCGAGCGTGGCATTGTCAAGGTTGGCGAAGAAGTC
GAGATCGTCGGCATCCGCGCGACCTCGAAGACGACGGTGACCGGCGTTGAAATGTTCCGCAAGCTGCTCGATCAGGGCCA
GGCCGGCGACAACATCGGCGCACTGGTTCGTGGTGTGAACCGTGACGGCGTCGAGCGTGGCCAGATCCTGTGCAAGCCGG
GCTCTGTCAAGCCGCACAAGAAGTTCATGGCGGAAGCCTACATCCTGACGAAGGAAGAGGGTGGCCGTCATACGCCGTTC
TTCACCAACTACCGTCCGCAGTTCTACTTCCGCACGACGGATGTGACCGGCATCGTGACGCTGCCTGAGGGCACCGAGAT
GGTCATGCCGGGCGACAACGTCACGGTTGCCGTCGAGCTGATCGTTCCGATCGCGATGGAAGAAAAGCTGCGCTTCGCAA
TCCGCGAAGGCGGCCGCACCGTCGGCGCCGGTATCGTTGCTTCGATCGTCGAGTAA

Upstream 100 bases:

>100_bases
AAATCCAGGCAAAGTATTCCGGTCAGAAGTGACCGGAATACCAATTGACCGATAACAAGAATTAGATCCCCTCGGGGACT
AGCAAAACGGAGAGCCGAAA

Downstream 100 bases:

>100_bases
TTCGCGGCTGGGCCACCAGGCCCAAGGAAAGGATAGGGACGCCGATCTCGGTGTCCCTCTCGATCTTTGAAACCGGTGGC
CCGCTAACGTAACTGAAGGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 2 [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGH
ADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPG
DDIPVVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIRATSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVNRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVAVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Sequences:

>Translated_391_residues
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGH
ADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPG
DDIPVVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
EIVGIRATSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVNRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPF
FTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVAVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE
>Mature_390_residues
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITISTAHVEYETPARHYAHVDCPGHA
DYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGD
DIPVVKGSALAALEDSDKKIGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEVE
IVGIRATSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVNRDGVERGQILCKPGSVKPHKKFMAEAYILTKEEGGRHTPFF
TNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVAVELIVPIAMEEKLRFAIREGGRTVGAGIVASIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=55.8375634517767, Blast_Score=475, Evalue=1e-134,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=29.1284403669725, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.1284403669725, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.3482142857143, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI54607086, Length=313, Percent_Identity=27.4760383386581, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI194018522, Length=320, Percent_Identity=29.375, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI194097354, Length=320, Percent_Identity=29.375, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI46094014, Length=322, Percent_Identity=30.4347826086957, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI194018520, Length=324, Percent_Identity=30.2469135802469, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=26.3940520446097, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=74.4303797468354, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=74.4303797468354, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=31.0160427807487, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI48994988, Length=249, Percent_Identity=32.1285140562249, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=211, Percent_Identity=31.2796208530806, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50.7462686567164, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.6692506459948, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=30.4444444444444, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=30.4444444444444, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=28.6046511627907, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17506081, Length=297, Percent_Identity=29.6296296296296, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=438, Percent_Identity=25.7990867579909, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI115532067, Length=445, Percent_Identity=26.0674157303371, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=445, Percent_Identity=26.0674157303371, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=166, Percent_Identity=33.7349397590361, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=277, Percent_Identity=25.9927797833935, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.0753768844221, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.316742081448, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.316742081448, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=273, Percent_Identity=26.7399267399267, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.9085545722714, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=269, Percent_Identity=27.5092936802974, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=137, Percent_Identity=30.6569343065693, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=244, Percent_Identity=27.0491803278689, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.7786259541985, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.7786259541985, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=49.7395833333333, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=28.7305122494432, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=28.7305122494432, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=28.7305122494432, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=28.7305122494432, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.6213808463252, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.6213808463252, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=299, Percent_Identity=31.7725752508361, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI45550900, Length=325, Percent_Identity=28.9230769230769, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=29.673590504451, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=25.6505576208178, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=25.6505576208178, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=31.7567567567568, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42541; Mature: 42410

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCEEEEE
AHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQ
EEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGDDIPVVKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHHCCCEE
EIVGIRATSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVNRDGVERGQILCKPGSVKPHK
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCHHH
KFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVAVEL
HHHHHEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EECHHHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKSKFERNKPHVNIGTIGHVDHGKTSLTAAITKYFGEFKAYDQIDAAPEEKARGITIST
CCCCCCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHHCCEEEEE
AHVEYETPARHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQ
EEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
VGVPAIVVFLNKVDQVDDAELLELVELEVRELLSSYDFPGDDIPVVKGSALAALEDSDKK
CCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEEECCCCHH
IGEDAIRELMAAVDAYIPTPERPIDQPFLMPIEDVFSISGRGTVVTGRVERGIVKVGEEV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECHHHHCHHHCCCEE
EIVGIRATSKTTVTGVEMFRKLLDQGQAGDNIGALVRGVNRDGVERGQILCKPGSVKPHK
EEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCCCHHH
KFMAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGIVTLPEGTEMVMPGDNVTVAVEL
HHHHHEEEEEECCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCEEEEEEE
IVPIAMEEKLRFAIREGGRTVGAGIVASIVE
EECHHHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA