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The map label for this gene is rlmB [H]

Identifier: 209548917

GI number: 209548917

Start: 1340111

End: 1341001

Strand: Reverse

Name: rlmB [H]

Synonym: Rleg2_1314

Alternate gene names: 209548917

Gene position: 1341001-1340111 (Counterclockwise)

Preceding gene: 209548929

Following gene: 209548916

Centisome position: 29.55

GC content: 64.42

Gene sequence:

>891_bases
ATGAGCAAAGACAAAAAACCCGGCGGCAAGGCCGCGACAGACCCATCGGCCAAGGATACGCATTACGCCACGCTGCGGCG
CGCCCACCGTGACGCCAAGCGCGAACGCGGCGAGATCCCGACCCCTCAGCCGCAGAAACGCAGGCGCGGGGCAGAGGACT
GGAAGCCGCCGGCGCTGGCGCCCGACCAGGTGCATCTCTATGGCCTGCATACGGTGCGCGCCGCCCTCGACAATCCCGAG
CGCAAGAAGATCAAACTCTTCGTGACACAGAACGCGCTGGCGCGCCTCGAAGTCGACGTCGAAACGCTCGGCATTCCCTT
CGAGATCGTCTCGCCGCAGGAGATCGACAAGGTGCTCGGCCCTGAGGCGATCCATCAGGGCGTCATGCTGGAAACGCGGC
CGCTGCCGGTCCGCCGGCTCGAAGCGCTGAAGGACAGCCCTCTCCTGCTCGTCCTCGACCAGGTCACCGATCCGCATAAT
GTCGGCGCGATCATGCGCTCGGCCGTCGCCTTCAATGCCGGCGCCGTCATCACCACCCAGAGACACAGCCCGACCGAGTC
AGGCGTGCTCGCCAAATCAGCCTCCGGCGCGCTCGAACTGATACCTTATATACAGGTGACCAATCTCGCCGATGCACTCG
GTGAACTGCACAAGCTCGGCTTCTCCACCATCGGCCTTGATTCGGAAGGGCCGGCGGCGCTCGAAGGTACGTTTTCGGGC
GAGAAAGTGGCGCTGGTGCTCGGCTCCGAGGGCAAGGGGCTTCGCCAGAAGACGCGGGAAACCGTCAACGCACTTGCCCG
TCTCGACATGCCCGGCGCGATCAAATCGCTGAACGTCTCGAATGCGGCGGCGATCGCGCTTTATGCGGCGCGGCTTTACC
TGAAGGCATAG

Upstream 100 bases:

>100_bases
CGATGACAACGGAAAAAACACGAAAAATTTCATTCGCCGCCGAAAGCCGGCATATGAAAGAAAGCCTATGCGGCGGCGAG
CGCCATGGCTATAAAGCCGC

Downstream 100 bases:

>100_bases
GCTGGTCGGCCTGCGGGCCGCGACAAGGGGGACTGTCATGATCCGTTTGGTCTTTCGGCTCGCGGCTTGCGGCGCCGCCC
TCCTCGCCTGCGATCAGACG

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSKDKKPGGKAATDPSAKDTHYATLRRAHRDAKRERGEIPTPQPQKRRRGAEDWKPPALAPDQVHLYGLHTVRAALDNPE
RKKIKLFVTQNALARLEVDVETLGIPFEIVSPQEIDKVLGPEAIHQGVMLETRPLPVRRLEALKDSPLLLVLDQVTDPHN
VGAIMRSAVAFNAGAVITTQRHSPTESGVLAKSASGALELIPYIQVTNLADALGELHKLGFSTIGLDSEGPAALEGTFSG
EKVALVLGSEGKGLRQKTRETVNALARLDMPGAIKSLNVSNAAAIALYAARLYLKA

Sequences:

>Translated_296_residues
MSKDKKPGGKAATDPSAKDTHYATLRRAHRDAKRERGEIPTPQPQKRRRGAEDWKPPALAPDQVHLYGLHTVRAALDNPE
RKKIKLFVTQNALARLEVDVETLGIPFEIVSPQEIDKVLGPEAIHQGVMLETRPLPVRRLEALKDSPLLLVLDQVTDPHN
VGAIMRSAVAFNAGAVITTQRHSPTESGVLAKSASGALELIPYIQVTNLADALGELHKLGFSTIGLDSEGPAALEGTFSG
EKVALVLGSEGKGLRQKTRETVNALARLDMPGAIKSLNVSNAAAIALYAARLYLKA
>Mature_295_residues
SKDKKPGGKAATDPSAKDTHYATLRRAHRDAKRERGEIPTPQPQKRRRGAEDWKPPALAPDQVHLYGLHTVRAALDNPER
KKIKLFVTQNALARLEVDVETLGIPFEIVSPQEIDKVLGPEAIHQGVMLETRPLPVRRLEALKDSPLLLVLDQVTDPHNV
GAIMRSAVAFNAGAVITTQRHSPTESGVLAKSASGALELIPYIQVTNLADALGELHKLGFSTIGLDSEGPAALEGTFSGE
KVALVLGSEGKGLRQKTRETVNALARLDMPGAIKSLNVSNAAAIALYAARLYLKA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=252, Percent_Identity=32.1428571428571, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790623, Length=238, Percent_Identity=36.1344537815126, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788935, Length=233, Percent_Identity=23.6051502145923, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=249, Percent_Identity=27.3092369477912, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=288, Percent_Identity=25.3472222222222, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31890; Mature: 31759

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDKKPGGKAATDPSAKDTHYATLRRAHRDAKRERGEIPTPQPQKRRRGAEDWKPPALA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
PDQVHLYGLHTVRAALDNPERKKIKLFVTQNALARLEVDVETLGIPFEIVSPQEIDKVLG
CCCEEEEEHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEHHHCCCCEEECCHHHHHHHCC
PEAIHQGVMLETRPLPVRRLEALKDSPLLLVLDQVTDPHNVGAIMRSAVAFNAGAVITTQ
HHHHHCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEC
RHSPTESGVLAKSASGALELIPYIQVTNLADALGELHKLGFSTIGLDSEGPAALEGTFSG
CCCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCC
EKVALVLGSEGKGLRQKTRETVNALARLDMPGAIKSLNVSNAAAIALYAARLYLKA
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKDKKPGGKAATDPSAKDTHYATLRRAHRDAKRERGEIPTPQPQKRRRGAEDWKPPALA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
PDQVHLYGLHTVRAALDNPERKKIKLFVTQNALARLEVDVETLGIPFEIVSPQEIDKVLG
CCCEEEEEHHHHHHHHCCCCCCEEEEEEECCCEEEEEEEHHHCCCCEEECCHHHHHHHCC
PEAIHQGVMLETRPLPVRRLEALKDSPLLLVLDQVTDPHNVGAIMRSAVAFNAGAVITTQ
HHHHHCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEC
RHSPTESGVLAKSASGALELIPYIQVTNLADALGELHKLGFSTIGLDSEGPAALEGTFSG
CCCCCCCCEEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCC
EKVALVLGSEGKGLRQKTRETVNALARLDMPGAIKSLNVSNAAAIALYAARLYLKA
CEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]