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The map label for this gene is birA [H]

Identifier: 209548878

GI number: 209548878

Start: 1293068

End: 1293832

Strand: Direct

Name: birA [H]

Synonym: Rleg2_1275

Alternate gene names: 209548878

Gene position: 1293068-1293832 (Clockwise)

Preceding gene: 209548877

Following gene: 209548879

Centisome position: 28.49

GC content: 63.53

Gene sequence:

>765_bases
ATGGCTTCCGACGGGCGGCGCCGGATATCGCTCGGCGATTTCAGGCACGAGGCTCTGTCGGAAACGTCGTCCACCAACAG
CGAATGCCTCGCCCGGGCTCGGGCGGGCGATGGCGGTAATCTCTGGGTGACGGCCGAACGCCAGACTGGCGGCCGCGGCC
GCCGCGGCCGGCTCTGGGTGTCCGAACGCGGCAATCTCTACGCCTCTCTATTGCTGATCGATCCGGCGCCGATGGAGCGC
CTGAGTTCCCTGCCGCTTGCCGTCGCCGTTGCCGTGCACCAGGCGATCCGCCAGGTGCTGCCGCCGGGCGCCGAGCCGCT
CGAGGTCAAATGGCCGAACGATATCCTCATCGGCCGTAAGAAGACCTGCGGCATTCTCGTCGAAGGCGAGAGGCTGCCGG
ACGGCCGTTACGCCCTGATCGTCGGTATCGGCATCAATGTCTCGGTCATGCCGGATAATCCGCTCTACCCCGTCACCTGC
CTGCGTCAGCATGCAAGTGCGGCGTCGCCGGAGGAACTCTTCGCCCATCTGTTCGCGGCGATGGCCGATGTGCTTGATCA
ATGGGATGAGGGCCGCGGTATTGCCGAGATCACCGCGCGCTGGCGCGCCATCGCCTGCGGCATCGGCGAAAAGATCACGG
TGAATTTGCCGGACCGATCGATTTCCGGACAATTCGCCGGAATTGATGATAATGGCTTGTTGATGCTCGATACCGGCACT
GGCAGGATAATGCCCATTGCTGCCGGTGATGTGTTTTTTGGATAG

Upstream 100 bases:

>100_bases
GCCTGGTCTTCGGCCTCTCCGGTCTCTTCGTCACTGCCTATGTCCTGATCGGCGGCCCGATCGGCGGCGCGGCAGAGCTT
GCCGCCGCGACGCTCTTTTG

Downstream 100 bases:

>100_bases
CGGAAAAAACGAAAATTATGGCGAAACAGGACGAGTTGGTATTCCTGCCTCTGGGCGGCGTTGGCGAGATCGGCATGAAT
CTCGCTCTTTATGGCTACGG

Product: biotin/acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MASDGRRRISLGDFRHEALSETSSTNSECLARARAGDGGNLWVTAERQTGGRGRRGRLWVSERGNLYASLLLIDPAPMER
LSSLPLAVAVAVHQAIRQVLPPGAEPLEVKWPNDILIGRKKTCGILVEGERLPDGRYALIVGIGINVSVMPDNPLYPVTC
LRQHASAASPEELFAHLFAAMADVLDQWDEGRGIAEITARWRAIACGIGEKITVNLPDRSISGQFAGIDDNGLLMLDTGT
GRIMPIAAGDVFFG

Sequences:

>Translated_254_residues
MASDGRRRISLGDFRHEALSETSSTNSECLARARAGDGGNLWVTAERQTGGRGRRGRLWVSERGNLYASLLLIDPAPMER
LSSLPLAVAVAVHQAIRQVLPPGAEPLEVKWPNDILIGRKKTCGILVEGERLPDGRYALIVGIGINVSVMPDNPLYPVTC
LRQHASAASPEELFAHLFAAMADVLDQWDEGRGIAEITARWRAIACGIGEKITVNLPDRSISGQFAGIDDNGLLMLDTGT
GRIMPIAAGDVFFG
>Mature_253_residues
ASDGRRRISLGDFRHEALSETSSTNSECLARARAGDGGNLWVTAERQTGGRGRRGRLWVSERGNLYASLLLIDPAPMERL
SSLPLAVAVAVHQAIRQVLPPGAEPLEVKWPNDILIGRKKTCGILVEGERLPDGRYALIVGIGINVSVMPDNPLYPVTCL
RQHASAASPEELFAHLFAAMADVLDQWDEGRGIAEITARWRAIACGIGEKITVNLPDRSISGQFAGIDDNGLLMLDTGTG
RIMPIAAGDVFFG

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=235, Percent_Identity=28.936170212766, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27364; Mature: 27232

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDGRRRISLGDFRHEALSETSSTNSECLARARAGDGGNLWVTAERQTGGRGRRGRLWV
CCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEE
SERGNLYASLLLIDPAPMERLSSLPLAVAVAVHQAIRQVLPPGAEPLEVKWPNDILIGRK
ECCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECC
KTCGILVEGERLPDGRYALIVGIGINVSVMPDNPLYPVTCLRQHASAASPEELFAHLFAA
CCCEEEEECCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
MADVLDQWDEGRGIAEITARWRAIACGIGEKITVNLPDRSISGQFAGIDDNGLLMLDTGT
HHHHHHHHHCCCCHHHHHHHHEEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEEECCC
GRIMPIAAGDVFFG
CEEEEEECCCCCCC
>Mature Secondary Structure 
ASDGRRRISLGDFRHEALSETSSTNSECLARARAGDGGNLWVTAERQTGGRGRRGRLWV
CCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEE
SERGNLYASLLLIDPAPMERLSSLPLAVAVAVHQAIRQVLPPGAEPLEVKWPNDILIGRK
ECCCCEEEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECC
KTCGILVEGERLPDGRYALIVGIGINVSVMPDNPLYPVTCLRQHASAASPEELFAHLFAA
CCCEEEEECCCCCCCCEEEEEEECCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
MADVLDQWDEGRGIAEITARWRAIACGIGEKITVNLPDRSISGQFAGIDDNGLLMLDTGT
HHHHHHHHHCCCCHHHHHHHHEEEEECCCCEEEEECCCCCCCCEEECCCCCCEEEEECCC
GRIMPIAAGDVFFG
CEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]