Click here to switch to the map view.

The map label for this gene is nuoI [H]

Identifier: 209548872

GI number: 209548872

Start: 1286508

End: 1286999

Strand: Direct

Name: nuoI [H]

Synonym: Rleg2_1269

Alternate gene names: 209548872

Gene position: 1286508-1286999 (Clockwise)

Preceding gene: 209548871

Following gene: 209548873

Centisome position: 28.35

GC content: 59.76

Gene sequence:

>492_bases
ATGGGAAGCTTGTCCAGCTCCATCAGCTCGCTGTTCCTCAAGGAATTCTTCGGCGCGTTCTTTCTGTCGATGCGCTATTT
CTTCCGCCAGAAGGCGACCATCAACTATCCCTTCGAAAAGGGCCCGGTTTCCCCGCGTTTCCGTGGTGAGCATGCGTTGC
GCCGTTATCCGAACGGCGAGGAACGCTGCATCGCCTGCAAACTCTGCGAGGCGATCTGTCCTGCCCAGGCCATCACCATC
GAGGCCGGCCCCCGCCGCAACGACGGCACCCGCCGCACGGTGCGCTACGACATCGATATGGTGAAGTGCATCTATTGCGG
CTTCTGCCAGGAGGCCTGCCCGGTCGATGCGATCGTCGAAGGTCCGAATTTCGAATTTGCGACGGAAACCCGCGAAGAAC
TCTATTTCGACAAGGCGCGCCTTCTCGACAACGGAGACCGGTGGGAGCGTGAAATCGCCCGTAACATTGCGATCGACTCG
CCGTACCGCTGA

Upstream 100 bases:

>100_bases
CTGTCGCTCGCCATGGTCATCATCGTTGCATTCGTGCTGAAGCTGATGGGATGGGCATGATGACGCCCTCTCTCGTTTCC
GGCAAAATTGGAGGTTGAAG

Downstream 100 bases:

>100_bases
TCGAGTTTTGAAATGCCGCCAGGGGGCTTCGCTGCTCCGGTCGCGGTCAATATGCACAGGAGCTTTGCCGGTCAGCCGCG
CAAACTCTATCGGGCAGAAA

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MGSLSSSISSLFLKEFFGAFFLSMRYFFRQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKARLLDNGDRWEREIARNIAIDS
PYR

Sequences:

>Translated_163_residues
MGSLSSSISSLFLKEFFGAFFLSMRYFFRQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITI
EAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKARLLDNGDRWEREIARNIAIDS
PYR
>Mature_162_residues
GSLSSSISSLFLKEFFGAFFLSMRYFFRQKATINYPFEKGPVSPRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIE
AGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELYFDKARLLDNGDRWEREIARNIAIDSP
YR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=154, Percent_Identity=74.6753246753247, Blast_Score=246, Evalue=7e-66,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=36.6197183098592, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17555194, Length=162, Percent_Identity=67.9012345679012, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17864306, Length=157, Percent_Identity=71.3375796178344, Blast_Score=241, Evalue=1e-64,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 18825; Mature: 18693

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLSSSISSLFLKEFFGAFFLSMRYFFRQKATINYPFEKGPVSPRFRGEHALRRYPNGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYFDKARLLDNGDRWEREIARNIAIDSPYR
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GSLSSSISSLFLKEFFGAFFLSMRYFFRQKATINYPFEKGPVSPRFRGEHALRRYPNGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHCCCCC
ERCIACKLCEAICPAQAITIEAGPRRNDGTRRTVRYDIDMVKCIYCGFCQEACPVDAIVE
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEC
GPNFEFATETREELYFDKARLLDNGDRWEREIARNIAIDSPYR
CCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]