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The map label for this gene is btuE [H]

Identifier: 209548860

GI number: 209548860

Start: 1274600

End: 1275148

Strand: Direct

Name: btuE [H]

Synonym: Rleg2_1257

Alternate gene names: 209548860

Gene position: 1274600-1275148 (Clockwise)

Preceding gene: 209548859

Following gene: 209548862

Centisome position: 28.09

GC content: 59.74

Gene sequence:

>549_bases
ATGGCGGGCAATGTGCTGGATATCCCTGTCAAATCAGTGGATGGTCGTGAAACGACACTCAGCGAATACAAGGGCAGGGT
GCTCTTGGTCGTCAACGTTGCCTCGAAATGCGGGCTGACCGTTCAATATGAGGGCCTCGAAAAGCTCTACGGCGAAAAGC
GCGAGCGCGGCTTCGTCATCGCCGCTTTCCCCGCCAACGACTTCAAGGGCCAGGAGCCCGGCACCGACGCCGAGATCCTC
GACTTCTGCACCAGCACCTACGACGTCACCTTCCCGATCTTCTCGAAGATTTCGGTCAAGGGCGAGACCCAGCACCCCCT
CTACCGGCAACTGACCAAATCGGGCGTGAAGACCACAGGTGACGGCCCGATGCGCGAACGCCTGAAATCCCACGGCATAT
CGGGCGGCGACGAGGACGATATCCTCTGGAACTTCGAAAAATTCCTGATCGGCCGCGACGGTAAAGTCGCTGCCCGATTC
GCCCCCGATGTGACGGCCGATGATTCGCGGCTGGTTTCGGCGGTGGATAAGGAACTGGCGAAGGGGTGA

Upstream 100 bases:

>100_bases
CACCGGCGTCCTGCTAACGCCGAGTTGATTCCGACGTTGATGACAACGGCGGCGATATCGCTAAGTTGGCGAGACATATC
TTATCAATTTGGAGGTTCCC

Downstream 100 bases:

>100_bases
AATGGCGGACGATCGGCCTCGACCGTCTCCCGATTCCAGAACGCCACTTCGGAATCCCGAAATTCCCCGCTTCGGGATTC
CGAAATTCGCCGAGCTTTGC

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MAGNVLDIPVKSVDGRETTLSEYKGRVLLVVNVASKCGLTVQYEGLEKLYGEKRERGFVIAAFPANDFKGQEPGTDAEIL
DFCTSTYDVTFPIFSKISVKGETQHPLYRQLTKSGVKTTGDGPMRERLKSHGISGGDEDDILWNFEKFLIGRDGKVAARF
APDVTADDSRLVSAVDKELAKG

Sequences:

>Translated_182_residues
MAGNVLDIPVKSVDGRETTLSEYKGRVLLVVNVASKCGLTVQYEGLEKLYGEKRERGFVIAAFPANDFKGQEPGTDAEIL
DFCTSTYDVTFPIFSKISVKGETQHPLYRQLTKSGVKTTGDGPMRERLKSHGISGGDEDDILWNFEKFLIGRDGKVAARF
APDVTADDSRLVSAVDKELAKG
>Mature_181_residues
AGNVLDIPVKSVDGRETTLSEYKGRVLLVVNVASKCGLTVQYEGLEKLYGEKRERGFVIAAFPANDFKGQEPGTDAEILD
FCTSTYDVTFPIFSKISVKGETQHPLYRQLTKSGVKTTGDGPMRERLKSHGISGGDEDDILWNFEKFLIGRDGKVAARFA
PDVTADDSRLVSAVDKELAKG

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=165, Percent_Identity=36.969696969697, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI75709200, Length=162, Percent_Identity=38.8888888888889, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI90903240, Length=162, Percent_Identity=38.8888888888889, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI192455698, Length=155, Percent_Identity=36.7741935483871, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI90903238, Length=148, Percent_Identity=38.5135135135135, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI6006001, Length=195, Percent_Identity=33.8461538461538, Blast_Score=90, Evalue=9e-19,
Organism=Homo sapiens, GI4557629, Length=174, Percent_Identity=32.183908045977, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI33186887, Length=165, Percent_Identity=33.9393939393939, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI41406084, Length=154, Percent_Identity=33.1168831168831, Blast_Score=77, Evalue=9e-15,
Organism=Homo sapiens, GI32967607, Length=169, Percent_Identity=31.3609467455621, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=49.1803278688525, Blast_Score=182, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI71993584, Length=178, Percent_Identity=35.9550561797753, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17506887, Length=166, Percent_Identity=38.5542168674699, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17535473, Length=169, Percent_Identity=36.6863905325444, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI193209493, Length=184, Percent_Identity=32.0652173913043, Blast_Score=106, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17550320, Length=185, Percent_Identity=31.3513513513514, Blast_Score=106, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI193210707, Length=166, Percent_Identity=34.9397590361446, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71993573, Length=96, Percent_Identity=40.625, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71999642, Length=78, Percent_Identity=41.025641025641, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319721, Length=161, Percent_Identity=42.2360248447205, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6322228, Length=147, Percent_Identity=44.2176870748299, Blast_Score=121, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322826, Length=153, Percent_Identity=39.2156862745098, Blast_Score=109, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24656772, Length=179, Percent_Identity=35.7541899441341, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24656777, Length=181, Percent_Identity=35.9116022099448, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24656767, Length=181, Percent_Identity=35.9116022099448, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI85726443, Length=171, Percent_Identity=35.672514619883, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 19923; Mature: 19792

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNVLDIPVKSVDGRETTLSEYKGRVLLVVNVASKCGLTVQYEGLEKLYGEKRERGFVI
CCCCEEECCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHCCHHHCCEEE
AAFPANDFKGQEPGTDAEILDFCTSTYDVTFPIFSKISVKGETQHPLYRQLTKSGVKTTG
EEECCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCC
DGPMRERLKSHGISGGDEDDILWNFEKFLIGRDGKVAARFAPDVTADDSRLVSAVDKELA
CCHHHHHHHHCCCCCCCCCCEEEEHHHHEECCCCCEEEEECCCCCCCHHHHHHHHHHHHH
KG
CC
>Mature Secondary Structure 
AGNVLDIPVKSVDGRETTLSEYKGRVLLVVNVASKCGLTVQYEGLEKLYGEKRERGFVI
CCCEEECCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCEEEECCHHHHHCCHHHCCEEE
AAFPANDFKGQEPGTDAEILDFCTSTYDVTFPIFSKISVKGETQHPLYRQLTKSGVKTTG
EEECCCCCCCCCCCCCHHHHHHHHCCCEEEEEEEEEEEECCCCCCHHHHHHHHCCCCCCC
DGPMRERLKSHGISGGDEDDILWNFEKFLIGRDGKVAARFAPDVTADDSRLVSAVDKELA
CCHHHHHHHHCCCCCCCCCCEEEEHHHHEECCCCCEEEEECCCCCCCHHHHHHHHHHHHH
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]