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The map label for this gene is mdeA [H]

Identifier: 209548845

GI number: 209548845

Start: 1254883

End: 1256166

Strand: Direct

Name: mdeA [H]

Synonym: Rleg2_1242

Alternate gene names: 209548845

Gene position: 1254883-1256166 (Clockwise)

Preceding gene: 209548844

Following gene: 209548846

Centisome position: 27.65

GC content: 63.86

Gene sequence:

>1284_bases
ATGGCCAAGAACGATCCGGGATTCAATACGTTGGCGATCCATGCCGGCGCCCAGCCCGACCCGGCGACCGGCGCGCGGGT
CACGCCGATCTATCAGACGACAGCCTTTGTCTTCGATGATTCCGACCATGCCGCCGCCCTCTTCGGCCTGCAGGCCTTCG
GCAACATCTACAGCCGCATCATGAACCCGACGCAGGCCGTGCTCGAGGAGCGTGTCGCAGCGCTCGAAGGCGGCACGGCG
GCGCTTGCCGTCGCCTCCGGCCATGCAGCGCAGATGATCGTCTTCCACACCATCATGCGCCCCGGCGAAAATTTCATCGC
CGCCAACAGGCTCTATGGCGGCTCGATCAACCAGTTCGGCCACGCCTTCGAGAATTTCGGCTGGCAGGTACGCTGGGCCG
ATCCCGCCGACCCGGCAAGCTTCGAAAGCCAGATCGATGACAAGACGCGCGGCATCTTCATCGAAAGCCTGGCCAATCCC
GGCGGCACCTTCGTCGATATCGCCGCCATCGCCGATGTCGCCCACCGCAACGGCCTGCCGCTGATCGTCGACAATACGAT
GGCGACACCCTATCTCATCCGCCCGATCGAGCACGGCGCCGATATCGTCGTGCATTCGCTGACGAAATTCCTCGGCGGCC
ACGGCAATTCCATGGGCGGCCTGATCGTCGATGGCGGCACTTTCGACTGGTCGAAATCCGGCAATTACCCGATGCTGTCG
AGCCCGCGGCCGGAATATAACGGCATGGTCCTGCACGCGACCTTCGGCAATTTCGCCTTTGCGATTGCTGCCCGCGTACT
CGGCCTACGCGATCTTGGCCCGGCGATCTCGCCCTTCAACGCCTTCCTGATCCTGACTGGGATCGAGACTCTACCGCTGC
GCATGCAGCGCCACAGCGACAATGCGATCGCCGTCGCAAAGTGGCTGAAGGCGCACAGCAAGATCGCCTGGGTGAATTAT
GCCGGCCTCGACGACGACCCGAACCATGCCCTGCAGCAGCGCTACTCGCCGAAAGGCGCAGGCTCCGTCTTCACCTTCGG
CGTCGAGGGCGGCTATGAGGCAGGCAAGGCGCTGGTCGAGGGGCTGCAGCTCTTCTCCCACCTTGCCAATATCGGCGACA
CCCGCTCACTGGTCATCCATCCGGCGTCGACGACGCACCGGCAGCTGAGCGACGAGCAGAAGATCGCCGCCGGCGCCGGG
CCCGACGTGGTCCGCCTTTCCGTCGGCATCGAGGACGTCAAGGACATCATCGCCGATCTCGAACAGGCGCTCTCGAAGAT
CTGA

Upstream 100 bases:

>100_bases
CGGATTTTCCACCGAACGCCCATGGGTTGAAACGGATCACGATTAACTTTCGCAAGCGACAGGCTATGATCCTGCCCGTC
AGCAACAACTGGAGGACGAC

Downstream 100 bases:

>100_bases
CATCGGAGAGGTCCGTGACGACCATTGCAAATTTCATCCCCTTCAGCATTGACGGCGTCGAACCGGAGTTCGGCGCCCCG
GAGCCCGGCCGGATCATCTC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MAKNDPGFNTLAIHAGAQPDPATGARVTPIYQTTAFVFDDSDHAAALFGLQAFGNIYSRIMNPTQAVLEERVAALEGGTA
ALAVASGHAAQMIVFHTIMRPGENFIAANRLYGGSINQFGHAFENFGWQVRWADPADPASFESQIDDKTRGIFIESLANP
GGTFVDIAAIADVAHRNGLPLIVDNTMATPYLIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLS
SPRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSDNAIAVAKWLKAHSKIAWVNY
AGLDDDPNHALQQRYSPKGAGSVFTFGVEGGYEAGKALVEGLQLFSHLANIGDTRSLVIHPASTTHRQLSDEQKIAAGAG
PDVVRLSVGIEDVKDIIADLEQALSKI

Sequences:

>Translated_427_residues
MAKNDPGFNTLAIHAGAQPDPATGARVTPIYQTTAFVFDDSDHAAALFGLQAFGNIYSRIMNPTQAVLEERVAALEGGTA
ALAVASGHAAQMIVFHTIMRPGENFIAANRLYGGSINQFGHAFENFGWQVRWADPADPASFESQIDDKTRGIFIESLANP
GGTFVDIAAIADVAHRNGLPLIVDNTMATPYLIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLS
SPRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSDNAIAVAKWLKAHSKIAWVNY
AGLDDDPNHALQQRYSPKGAGSVFTFGVEGGYEAGKALVEGLQLFSHLANIGDTRSLVIHPASTTHRQLSDEQKIAAGAG
PDVVRLSVGIEDVKDIIADLEQALSKI
>Mature_426_residues
AKNDPGFNTLAIHAGAQPDPATGARVTPIYQTTAFVFDDSDHAAALFGLQAFGNIYSRIMNPTQAVLEERVAALEGGTAA
LAVASGHAAQMIVFHTIMRPGENFIAANRLYGGSINQFGHAFENFGWQVRWADPADPASFESQIDDKTRGIFIESLANPG
GTFVDIAAIADVAHRNGLPLIVDNTMATPYLIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLSS
PRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSDNAIAVAKWLKAHSKIAWVNYA
GLDDDPNHALQQRYSPKGAGSVFTFGVEGGYEAGKALVEGLQLFSHLANIGDTRSLVIHPASTTHRQLSDEQKIAAGAGP
DVVRLSVGIEDVKDIIADLEQALSKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=420, Percent_Identity=32.1428571428571, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI299473759, Length=420, Percent_Identity=29.5238095238095, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25453487, Length=419, Percent_Identity=28.8782816229117, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI1790375, Length=412, Percent_Identity=29.126213592233, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789383, Length=430, Percent_Identity=23.0232558139535, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538055, Length=425, Percent_Identity=32, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=419, Percent_Identity=31.0262529832936, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=425, Percent_Identity=32, Blast_Score=184, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=43.5483870967742, Blast_Score=346, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6319307, Length=426, Percent_Identity=30.0469483568075, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=26.9662921348315, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=361, Percent_Identity=25.4847645429363, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321904, Length=432, Percent_Identity=25, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=32.7058823529412, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45591; Mature: 45460

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNDPGFNTLAIHAGAQPDPATGARVTPIYQTTAFVFDDSDHAAALFGLQAFGNIYSRI
CCCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
MNPTQAVLEERVAALEGGTAALAVASGHAAQMIVFHTIMRPGENFIAANRLYGGSINQFG
CCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
HAFENFGWQVRWADPADPASFESQIDDKTRGIFIESLANPGGTFVDIAAIADVAHRNGLP
HHHHHCCCEEEECCCCCCCHHHHHCCCHHHCEEEEECCCCCCCEEEHHHHHHHHHCCCCC
LIVDNTMATPYLIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
SPRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSD
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCC
NAIAVAKWLKAHSKIAWVNYAGLDDDPNHALQQRYSPKGAGSVFTFGVEGGYEAGKALVE
CHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
GLQLFSHLANIGDTRSLVIHPASTTHRQLSDEQKIAAGAGPDVVRLSVGIEDVKDIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHH
EQALSKI
HHHHHCC
>Mature Secondary Structure 
AKNDPGFNTLAIHAGAQPDPATGARVTPIYQTTAFVFDDSDHAAALFGLQAFGNIYSRI
CCCCCCCCEEEEECCCCCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
MNPTQAVLEERVAALEGGTAALAVASGHAAQMIVFHTIMRPGENFIAANRLYGGSINQFG
CCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHH
HAFENFGWQVRWADPADPASFESQIDDKTRGIFIESLANPGGTFVDIAAIADVAHRNGLP
HHHHHCCCEEEECCCCCCCHHHHHCCCHHHCEEEEECCCCCCCEEEHHHHHHHHHCCCCC
LIVDNTMATPYLIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLS
EEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
SPRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSD
CCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCC
NAIAVAKWLKAHSKIAWVNYAGLDDDPNHALQQRYSPKGAGSVFTFGVEGGYEAGKALVE
CHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHH
GLQLFSHLANIGDTRSLVIHPASTTHRQLSDEQKIAAGAGPDVVRLSVGIEDVKDIIADL
HHHHHHHHHCCCCCCEEEEECCCCHHHHCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHH
EQALSKI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]