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The map label for this gene is wcaJ [H]

Identifier: 209548825

GI number: 209548825

Start: 1233838

End: 1235403

Strand: Reverse

Name: wcaJ [H]

Synonym: Rleg2_1222

Alternate gene names: 209548825

Gene position: 1235403-1233838 (Counterclockwise)

Preceding gene: 209548826

Following gene: 209548824

Centisome position: 27.22

GC content: 62.39

Gene sequence:

>1566_bases
ATGAACAAGCTGGAAAAAGGCGATCAATTCGACGTGGAAGCACTGCGCAAGCAGGTCTCCGACATCGAAGTGCGCGGCAA
TGCGGGTCAGGAAAAATCGTCCGACCCGACTGAAATCAATCCCTATGCCCGGCAGATCGCCGAACAGTTCCGTGACGGAA
CCAAGTCGCCGGTGATCATCATCGGGCAGTTGCGGCTGCTGGAATTCCTGGCGCTCTTCGCGATCGCCCTGATCGCCCAC
TATTTTTGGCCCGGCGACGGCTACGATTCATCGCTGGTGCGAACCGGCATGGCGGCGATCGCCTCTGCCCTGACGGTCAT
CGGCCTGCAGCTTGGCGACACCTACACGATTCCGGCACTTCGGGCCAAGCTGCGGCTGATCCCCCGCATCCTGGGCTCGT
GGACGGTCGCGCTCCTCCTGACCGTCGGCCTGTTCGCGCTGGTTCGCGGCAGCACATGGGCGATGGTCGCCGACGCCTAT
CTGCCCTGGTTTGCGTCTGGCGCGCTCTTCCTGGCGGCCGAGCGTTTCCTCGTCGCCTACGGCATCCGCAACTGGGCGCG
CAACGGCATCATGGAGCGCCGCGCCGTGATCGTCGGCGGCGGCGAGCCGGCCAAGGAACTGATCCGCATCCTCGAGCAGC
AGGATGACAATGACATCCGCATCTGCGGCATCTTCGACGATCGCGGCGAAAAGCGCTCGCCGATCATGGTCGCCGGTTAT
CCGAAGCTCGGCACCGTGGCCGAACTGGTCGAATTCGTGCGATTGACGCGCATCGACATGCTGATCATCGCTCTGCCGCT
TTCGGCCGAGGCGCGTATCTACGACCTTTTGAAGAAGCTCTGGGTTCTGCCGGTCGATATCCGCCTTGCCGCGCATGCCA
ACCGGCTGCGCTTCCGGCCGCGCGCCTATTCGCATGTCGGCACGGTGCCGATGCTCGACATTTTCAAGATGCCGATCCGC
GACTGGGATTCCGTTGCCAAGCGCGGCTTCGACATCTTCTTCACCGTAATCGCGCTGGCGCTGCTCTGGCCGCTGATGGT
CGCGACCGCCATCGCCATCAAGGTGACCTCCGAAGGCCCGGTTTTCTTCATGCAGAAGCGCCACGGCTTCAACAATGAAA
TCATCAACGTCTTCAAGTTCCGTTCGATGTACACCAACATGTCCGACCCCACGGGCAAGGCTGCGGTGACCAAGGGCGAT
CCGCGCGTCACCCGCGTCGGCCGCTTCATCCGCAAGACCTCGATCGACGAATTGCCGCAGCTTTTCAACGTGCTGAGGGG
CGAGCTTTCGCTGGTCGGGCCGCGCCCGCATGCCGTTCTCGCCCAGGCCCGTGACCGCGCCTTCGGCGATGTCGTCGAGG
GCTATTTCGCCCGCCACCGCGTCAAGCCAGGGGTCACCGGCTGGGCGCAGATCAACGGCTGGCGCGGCGAAGTGGACAAT
GACGAGAAGATCAAGTTCCGCACCGCCTACGACCTCTATTACATCGAGAACTGGTCGCTCTGGTTCGATCTCAAGATCCT
GTTCCTGACGCCGATCCGGCTGCTCAACACGGAAAATGCCTATTGA

Upstream 100 bases:

>100_bases
GCTCGTTGATCCGGCCGCCGGACGAGCAATGCCCGCCGCCCCGTAAATATTTCTTAGGGGCTTTCTGGTAATTCGCTTGG
TGACAACGAGCGAAAGCCCC

Downstream 100 bases:

>100_bases
GCGCGATCGAGGCGACATATCCCCGTGTCGCCCAGCCGCAGCGGATGACGCTGCGCCTGATCGGCTCGGCCTTCGTCGCC
TTCGGCGTCTTCCTCTCCGG

Product: exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MNKLEKGDQFDVEALRKQVSDIEVRGNAGQEKSSDPTEINPYARQIAEQFRDGTKSPVIIIGQLRLLEFLALFAIALIAH
YFWPGDGYDSSLVRTGMAAIASALTVIGLQLGDTYTIPALRAKLRLIPRILGSWTVALLLTVGLFALVRGSTWAMVADAY
LPWFASGALFLAAERFLVAYGIRNWARNGIMERRAVIVGGGEPAKELIRILEQQDDNDIRICGIFDDRGEKRSPIMVAGY
PKLGTVAELVEFVRLTRIDMLIIALPLSAEARIYDLLKKLWVLPVDIRLAAHANRLRFRPRAYSHVGTVPMLDIFKMPIR
DWDSVAKRGFDIFFTVIALALLWPLMVATAIAIKVTSEGPVFFMQKRHGFNNEIINVFKFRSMYTNMSDPTGKAAVTKGD
PRVTRVGRFIRKTSIDELPQLFNVLRGELSLVGPRPHAVLAQARDRAFGDVVEGYFARHRVKPGVTGWAQINGWRGEVDN
DEKIKFRTAYDLYYIENWSLWFDLKILFLTPIRLLNTENAY

Sequences:

>Translated_521_residues
MNKLEKGDQFDVEALRKQVSDIEVRGNAGQEKSSDPTEINPYARQIAEQFRDGTKSPVIIIGQLRLLEFLALFAIALIAH
YFWPGDGYDSSLVRTGMAAIASALTVIGLQLGDTYTIPALRAKLRLIPRILGSWTVALLLTVGLFALVRGSTWAMVADAY
LPWFASGALFLAAERFLVAYGIRNWARNGIMERRAVIVGGGEPAKELIRILEQQDDNDIRICGIFDDRGEKRSPIMVAGY
PKLGTVAELVEFVRLTRIDMLIIALPLSAEARIYDLLKKLWVLPVDIRLAAHANRLRFRPRAYSHVGTVPMLDIFKMPIR
DWDSVAKRGFDIFFTVIALALLWPLMVATAIAIKVTSEGPVFFMQKRHGFNNEIINVFKFRSMYTNMSDPTGKAAVTKGD
PRVTRVGRFIRKTSIDELPQLFNVLRGELSLVGPRPHAVLAQARDRAFGDVVEGYFARHRVKPGVTGWAQINGWRGEVDN
DEKIKFRTAYDLYYIENWSLWFDLKILFLTPIRLLNTENAY
>Mature_521_residues
MNKLEKGDQFDVEALRKQVSDIEVRGNAGQEKSSDPTEINPYARQIAEQFRDGTKSPVIIIGQLRLLEFLALFAIALIAH
YFWPGDGYDSSLVRTGMAAIASALTVIGLQLGDTYTIPALRAKLRLIPRILGSWTVALLLTVGLFALVRGSTWAMVADAY
LPWFASGALFLAAERFLVAYGIRNWARNGIMERRAVIVGGGEPAKELIRILEQQDDNDIRICGIFDDRGEKRSPIMVAGY
PKLGTVAELVEFVRLTRIDMLIIALPLSAEARIYDLLKKLWVLPVDIRLAAHANRLRFRPRAYSHVGTVPMLDIFKMPIR
DWDSVAKRGFDIFFTVIALALLWPLMVATAIAIKVTSEGPVFFMQKRHGFNNEIINVFKFRSMYTNMSDPTGKAAVTKGD
PRVTRVGRFIRKTSIDELPQLFNVLRGELSLVGPRPHAVLAQARDRAFGDVVEGYFARHRVKPGVTGWAQINGWRGEVDN
DEKIKFRTAYDLYYIENWSLWFDLKILFLTPIRLLNTENAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=393, Percent_Identity=35.6234096692112, Blast_Score=239, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 58761; Mature: 58761

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLEKGDQFDVEALRKQVSDIEVRGNAGQEKSSDPTEINPYARQIAEQFRDGTKSPVII
CCCCCCCCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
IGQLRLLEFLALFAIALIAHYFWPGDGYDSSLVRTGMAAIASALTVIGLQLGDTYTIPAL
EEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHH
RAKLRLIPRILGSWTVALLLTVGLFALVRGSTWAMVADAYLPWFASGALFLAAERFLVAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GIRNWARNGIMERRAVIVGGGEPAKELIRILEQQDDNDIRICGIFDDRGEKRSPIMVAGY
HHHHHHHCCHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECC
PKLGTVAELVEFVRLTRIDMLIIALPLSAEARIYDLLKKLWVLPVDIRLAAHANRLRFRP
CCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHCCCC
RAYSHVGTVPMLDIFKMPIRDWDSVAKRGFDIFFTVIALALLWPLMVATAIAIKVTSEGP
CHHHHCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
VFFMQKRHGFNNEIINVFKFRSMYTNMSDPTGKAAVTKGDPRVTRVGRFIRKTSIDELPQ
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHH
LFNVLRGELSLVGPRPHAVLAQARDRAFGDVVEGYFARHRVKPGVTGWAQINGWRGEVDN
HHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DEKIKFRTAYDLYYIENWSLWFDLKILFLTPIRLLNTENAY
CCEEEEEEEEEEEEEECCEEEEEEEEHHHCCHHHCCCCCCC
>Mature Secondary Structure
MNKLEKGDQFDVEALRKQVSDIEVRGNAGQEKSSDPTEINPYARQIAEQFRDGTKSPVII
CCCCCCCCCCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEE
IGQLRLLEFLALFAIALIAHYFWPGDGYDSSLVRTGMAAIASALTVIGLQLGDTYTIPAL
EEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECHHH
RAKLRLIPRILGSWTVALLLTVGLFALVRGSTWAMVADAYLPWFASGALFLAAERFLVAY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
GIRNWARNGIMERRAVIVGGGEPAKELIRILEQQDDNDIRICGIFDDRGEKRSPIMVAGY
HHHHHHHCCHHHCCEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEECC
PKLGTVAELVEFVRLTRIDMLIIALPLSAEARIYDLLKKLWVLPVDIRLAAHANRLRFRP
CCCCHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCHHHCCCC
RAYSHVGTVPMLDIFKMPIRDWDSVAKRGFDIFFTVIALALLWPLMVATAIAIKVTSEGP
CHHHHCCCCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCC
VFFMQKRHGFNNEIINVFKFRSMYTNMSDPTGKAAVTKGDPRVTRVGRFIRKTSIDELPQ
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHH
LFNVLRGELSLVGPRPHAVLAQARDRAFGDVVEGYFARHRVKPGVTGWAQINGWRGEVDN
HHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
DEKIKFRTAYDLYYIENWSLWFDLKILFLTPIRLLNTENAY
CCEEEEEEEEEEEEEECCEEEEEEEEHHHCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]