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The map label for this gene is hemB [H]

Identifier: 209548716

GI number: 209548716

Start: 1138094

End: 1139107

Strand: Reverse

Name: hemB [H]

Synonym: Rleg2_1113

Alternate gene names: 209548716

Gene position: 1139107-1138094 (Counterclockwise)

Preceding gene: 209548720

Following gene: 209548715

Centisome position: 25.1

GC content: 62.72

Gene sequence:

>1014_bases
ATGCAGGATAGGACGCATCTCGTCGACGACATCACCGGCCATCGCCGCATGCGGCGCAACCGCAAGGCGGAATGGACACG
CCGGTTGGTGCAGGAGAACCGGCTGACGGTCGACGACCTGATCTGGCCGATCTTCATCGTGCCCGGCTCCGGCATCGTCG
ATCCGATCCCGGCCATGCCGGGCGTCAACCGCATGAGCATCGACAAGGCCGTCGAGGCAGCACGCGAAGCGGCCGGCCTC
GGCATTCCAGCGCTCGCGACCTTTCCGAATATCGAGATGGAACTACGCGACGAAACGGGCTCGAACAGCCTCGAAGCCAA
TAATCTGATCAATAAGGCGACGTCGGCGATCAAGAAGGCGGTGCCCAATATCGGCGTCATCACCGACGTCGCGCTCGATC
CCTTCACCAGCCATGGCCATGACGGCATTTTGAGAGGCAGCGAGATCGTCAACGACGAGACGGTCGATCAGGTGGCGCGT
GCCGCCGTGATGCAGGCGGATGCCGGCGCCGACATCATTGCGCCGTCCGAGATGATGGACGGGCGCATCGGCGCAATCCG
CATGGCGCTTGATGCAGCCGGCCACCAGAGCGTCGGCATCATGAGCTATGCGACGAAATTCGCTTCCGCCTTCTATGGCC
CCTATCGCGAGGCGATCTCGACGGGCGGGCTGCTGAAGGGTGACAAGAAGACCTATTATATCGACCCGGCCAACGGCACC
GAGGCGATCCGCGATGCGGCCCTCGACGTCGAGGAAGGTGCTGATATGCTGATGGTTAAGCCCGGCCTGCCCTATCTCGA
CATCTGCTGGCGGATGAAGGAGGCCTTCGGCCTGCCGACCTTCGCCTACCAGGTTTCCGGCGAATATTCGCAGATCAAGG
CAGCGGCGATGAATGGCTGGATCGACGGCGAGCGGGCGATGCTCGAAACGCTGCTGTCGTTCAAGCGGGCGGGATGCGAC
GGCATCCTCACCTATTTCGCGGTCGAGGTGGCGAAAGTCCTCGCGAAGCGGTGA

Upstream 100 bases:

>100_bases
GCCTCAAGTTTTTCCGCCGCGGCCAATCGTCCGGTTTGGATTTTTCGGGGGTCATGATAATGAGCGCTCCGATAAATGGA
ATGGGCCAGGGGCAGATATC

Downstream 100 bases:

>100_bases
TTGCGAGCATGATGCCGAAAAGTGTATGCGGTTTTCGGGTGACATCATGCTCTATTTCTTCGATTTAGATGCGGATGTTT
CAGGTCGGATCGGCCTGAAA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQDRTHLVDDITGHRRMRRNRKAEWTRRLVQENRLTVDDLIWPIFIVPGSGIVDPIPAMPGVNRMSIDKAVEAAREAAGL
GIPALATFPNIEMELRDETGSNSLEANNLINKATSAIKKAVPNIGVITDVALDPFTSHGHDGILRGSEIVNDETVDQVAR
AAVMQADAGADIIAPSEMMDGRIGAIRMALDAAGHQSVGIMSYATKFASAFYGPYREAISTGGLLKGDKKTYYIDPANGT
EAIRDAALDVEEGADMLMVKPGLPYLDICWRMKEAFGLPTFAYQVSGEYSQIKAAAMNGWIDGERAMLETLLSFKRAGCD
GILTYFAVEVAKVLAKR

Sequences:

>Translated_337_residues
MQDRTHLVDDITGHRRMRRNRKAEWTRRLVQENRLTVDDLIWPIFIVPGSGIVDPIPAMPGVNRMSIDKAVEAAREAAGL
GIPALATFPNIEMELRDETGSNSLEANNLINKATSAIKKAVPNIGVITDVALDPFTSHGHDGILRGSEIVNDETVDQVAR
AAVMQADAGADIIAPSEMMDGRIGAIRMALDAAGHQSVGIMSYATKFASAFYGPYREAISTGGLLKGDKKTYYIDPANGT
EAIRDAALDVEEGADMLMVKPGLPYLDICWRMKEAFGLPTFAYQVSGEYSQIKAAAMNGWIDGERAMLETLLSFKRAGCD
GILTYFAVEVAKVLAKR
>Mature_337_residues
MQDRTHLVDDITGHRRMRRNRKAEWTRRLVQENRLTVDDLIWPIFIVPGSGIVDPIPAMPGVNRMSIDKAVEAAREAAGL
GIPALATFPNIEMELRDETGSNSLEANNLINKATSAIKKAVPNIGVITDVALDPFTSHGHDGILRGSEIVNDETVDQVAR
AAVMQADAGADIIAPSEMMDGRIGAIRMALDAAGHQSVGIMSYATKFASAFYGPYREAISTGGLLKGDKKTYYIDPANGT
EAIRDAALDVEEGADMLMVKPGLPYLDICWRMKEAFGLPTFAYQVSGEYSQIKAAAMNGWIDGERAMLETLLSFKRAGCD
GILTYFAVEVAKVLAKR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=303, Percent_Identity=38.6138613861386, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=45.5974842767296, Blast_Score=253, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=309, Percent_Identity=35.2750809061489, Blast_Score=162, Evalue=8e-41,
Organism=Drosophila melanogaster, GI21358291, Length=314, Percent_Identity=37.2611464968153, Blast_Score=220, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36617; Mature: 36617

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDRTHLVDDITGHRRMRRNRKAEWTRRLVQENRLTVDDLIWPIFIVPGSGIVDPIPAMP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCCCCCCCCCCCC
GVNRMSIDKAVEAAREAAGLGIPALATFPNIEMELRDETGSNSLEANNLINKATSAIKKA
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
VPNIGVITDVALDPFTSHGHDGILRGSEIVNDETVDQVARAAVMQADAGADIIAPSEMMD
CCCCCEEEHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
GRIGAIRMALDAAGHQSVGIMSYATKFASAFYGPYREAISTGGLLKGDKKTYYIDPANGT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCH
EAIRDAALDVEEGADMLMVKPGLPYLDICWRMKEAFGLPTFAYQVSGEYSQIKAAAMNGW
HHHHHHHCCHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCC
IDGERAMLETLLSFKRAGCDGILTYFAVEVAKVLAKR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQDRTHLVDDITGHRRMRRNRKAEWTRRLVQENRLTVDDLIWPIFIVPGSGIVDPIPAMP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEEECCCCCCCCCCCCC
GVNRMSIDKAVEAAREAAGLGIPALATFPNIEMELRDETGSNSLEANNLINKATSAIKKA
CCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
VPNIGVITDVALDPFTSHGHDGILRGSEIVNDETVDQVARAAVMQADAGADIIAPSEMMD
CCCCCEEEHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
GRIGAIRMALDAAGHQSVGIMSYATKFASAFYGPYREAISTGGLLKGDKKTYYIDPANGT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCH
EAIRDAALDVEEGADMLMVKPGLPYLDICWRMKEAFGLPTFAYQVSGEYSQIKAAAMNGW
HHHHHHHCCHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCC
IDGERAMLETLLSFKRAGCDGILTYFAVEVAKVLAKR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]