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The map label for this gene is ptrB [C]

Identifier: 209548676

GI number: 209548676

Start: 1092403

End: 1094445

Strand: Reverse

Name: ptrB [C]

Synonym: Rleg2_1073

Alternate gene names: 209548676

Gene position: 1094445-1092403 (Counterclockwise)

Preceding gene: 209548677

Following gene: 209548675

Centisome position: 24.12

GC content: 64.12

Gene sequence:

>2043_bases
ATGAACCTTCATCTCGAAACGGACGACGATCCGCGCACCCGCCAGTTCATCGACGATCATAATCGCATTTCCGATGCGGC
GTTCAGAACGGCTGAATTTGCCAGGGATCGCGACGCGATCAAGGCGTTGATCGAGCGGCAGGACAGGTTGATCGTGCCGA
TGCGCCGCGGTGCGTGGTTGTTCGATTTTCGGCAGAGCAAGGACAATCCTCTCGGCGTCTGGCTCCGTCTGCCTGCCGAC
CAGGAGCCGCTGCCGGACGCCGGTTGGGAACCGGTTTTCGATCTCGATGCCTTCTGCATCCGTGATGGCAAGCGCTGGAA
CTGGCGCGGCGTCGTCACCTGCCCGTGGGAGCCGACGCGGGTGCTGCTCACGCTTTCGGACGGCGGCTCGGATCTTCTCC
GGCTGCTCGAATTCGACGCCGAGAAGAAAGAGATCGTCGAGGGCGGTTTCGATACGCCGGCCGCACGATCGCATGCCAAT
TGGCTGAGCCGTGATGAGATCTGCTATTTCGGCTCGACCGACCGATTTTCGGCGACCCGCTCCGGATGGCCACGGGTCGG
GCGGCGATTGAAGCGCGGGCAGCGGCCGGAGGATGCCGCCGTCATGTTCGAAGCGGCGAATGAAGACGTCTACGGCTTCA
ATCTCGTCATCGATCCTGCCCTGTCAGGCGGTTCGGCGGATGCCGGATTGATCGACATTTTCGTCGCCGCCCATGAGATC
GGCGTCGCCAGCGCTTTCCTGACCACCGCCGACGGCACGCGGCGGCGCATCGATCTGCCTGAGGAGGCCGACTTCCAGTT
CAATCACGATCATTGCCTCTGGCGGGCAAAGACCGATGAGCGTGTTGCCACCGGCAGCCTCGTGCTGCAGCGCTTCGATC
CCGGCTCGGAGACGGCTCCGCTCGGGCCGGCGCGGATCCTGTTTCAGCCTGGCGAGGGCCAGTCGATCTCGCAGATGATG
CTGATGCGCGAGTGGTGCGTCTTCATCATTTCCGATCGGCTGCGTCCGCGTCTCATGGTGCTGGATCTGACCAAGCCCGA
TGCCGAACAGCGCGAGATCGTGCTGCCTGCCGACATGCAGACGGCTCATTTCAGGCCGCTTTATGCGGATCTGCATCTTG
GCGACGATACGCTCTGCCTCATCGGCCAGGGCTTCCTGCAGCCGCCGGCCTGTTACCGGCTCGAGCTATCGGATCGCAAC
AAGGCGGCCGAGCCGGTTTTCATCGCCGCAGCACCCAGCTATTTCGATGCGGCCGGTATGTCATCCGAGCTGCTGGAGGC
GGTTTCCGAGGATGGAACCAGGGTTACCTACCGGCTGGTCCTGCCGAAACACTGGACCAAGGGCGCGCTGCCGGTGCTGC
TTTACGGCTATGGCGGCTTCGATGTTTCGCTGTCGCCGAGCTATTCCGGCGTGACGGGACGCTGGCTGGAACAGGGCGGC
GCCTATGTGCAGGCCTATATCCGCGGCGGCGGCGAATTCGGCCCCGATTGGTATCGCAGCGCCAAGCGGCAGGGGCGAGA
CCGGGCGTTCGCCGATTTCGTCGCCATCGCCCGCGATCTCGTCGCCCGCGGCTACACCGTGCCGTCGCGCATCGCCTGCC
AGGGCGGCAGCAATGGCGGCCTGCTGACCGGCGTGATGCTGACGCGCTATCCGGACGATTTCGGCGCCGTCTGGTGCCAG
GTGCCGGTGCTCGACATGACACGCTTCCACTTGTTCAGCGCAGGCCAGGCCTGGATGGACGAATATGGCGATCCGGAGGT
AGCAGCGGATCGGGATTTCATGCTCGGCTATTCGCCGCTCCATAATGTCCGGCCGGCGACCGAGGTCACCTATCCGCCGA
TCTATATCGAAAGCTCCGCCAACGACGACCGGGTGCATCCCTCGCATGCGCGCCGCTTCGCCGCGCGGCTGGAGGAAGCG
GGACACCGGCCGTTCTTCCATGAGTTCGGCTCGGGCGGGCATGGCGGCGACGGCAATTCCGAAGAGCGCGCCGCCCGGGC
GGCGATGGGTTATAGCTTCCTTCGCCAAACTATCATGACGTAA

Upstream 100 bases:

>100_bases
CGTCGCTTCTGCCGATTTGGTGACGACCCGGTAGGCCGGGTTGCCAAGTCCGGACGCGCTGCCTAGATCTGTTCGACGTC
AACGAACCGGCAGGTCAGCT

Downstream 100 bases:

>100_bases
GACGGGCCATCATGCGGTAGAATAGAAGAGAATGAAGGGGAGAGCTTGTCGGCGCTCTCCTTTCTGCTAAAAGAAGCGCA
ATAAATCATACTTTTCACAG

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MNLHLETDDDPRTRQFIDDHNRISDAAFRTAEFARDRDAIKALIERQDRLIVPMRRGAWLFDFRQSKDNPLGVWLRLPAD
QEPLPDAGWEPVFDLDAFCIRDGKRWNWRGVVTCPWEPTRVLLTLSDGGSDLLRLLEFDAEKKEIVEGGFDTPAARSHAN
WLSRDEICYFGSTDRFSATRSGWPRVGRRLKRGQRPEDAAVMFEAANEDVYGFNLVIDPALSGGSADAGLIDIFVAAHEI
GVASAFLTTADGTRRRIDLPEEADFQFNHDHCLWRAKTDERVATGSLVLQRFDPGSETAPLGPARILFQPGEGQSISQMM
LMREWCVFIISDRLRPRLMVLDLTKPDAEQREIVLPADMQTAHFRPLYADLHLGDDTLCLIGQGFLQPPACYRLELSDRN
KAAEPVFIAAAPSYFDAAGMSSELLEAVSEDGTRVTYRLVLPKHWTKGALPVLLYGYGGFDVSLSPSYSGVTGRWLEQGG
AYVQAYIRGGGEFGPDWYRSAKRQGRDRAFADFVAIARDLVARGYTVPSRIACQGGSNGGLLTGVMLTRYPDDFGAVWCQ
VPVLDMTRFHLFSAGQAWMDEYGDPEVAADRDFMLGYSPLHNVRPATEVTYPPIYIESSANDDRVHPSHARRFAARLEEA
GHRPFFHEFGSGGHGGDGNSEERAARAAMGYSFLRQTIMT

Sequences:

>Translated_680_residues
MNLHLETDDDPRTRQFIDDHNRISDAAFRTAEFARDRDAIKALIERQDRLIVPMRRGAWLFDFRQSKDNPLGVWLRLPAD
QEPLPDAGWEPVFDLDAFCIRDGKRWNWRGVVTCPWEPTRVLLTLSDGGSDLLRLLEFDAEKKEIVEGGFDTPAARSHAN
WLSRDEICYFGSTDRFSATRSGWPRVGRRLKRGQRPEDAAVMFEAANEDVYGFNLVIDPALSGGSADAGLIDIFVAAHEI
GVASAFLTTADGTRRRIDLPEEADFQFNHDHCLWRAKTDERVATGSLVLQRFDPGSETAPLGPARILFQPGEGQSISQMM
LMREWCVFIISDRLRPRLMVLDLTKPDAEQREIVLPADMQTAHFRPLYADLHLGDDTLCLIGQGFLQPPACYRLELSDRN
KAAEPVFIAAAPSYFDAAGMSSELLEAVSEDGTRVTYRLVLPKHWTKGALPVLLYGYGGFDVSLSPSYSGVTGRWLEQGG
AYVQAYIRGGGEFGPDWYRSAKRQGRDRAFADFVAIARDLVARGYTVPSRIACQGGSNGGLLTGVMLTRYPDDFGAVWCQ
VPVLDMTRFHLFSAGQAWMDEYGDPEVAADRDFMLGYSPLHNVRPATEVTYPPIYIESSANDDRVHPSHARRFAARLEEA
GHRPFFHEFGSGGHGGDGNSEERAARAAMGYSFLRQTIMT
>Mature_680_residues
MNLHLETDDDPRTRQFIDDHNRISDAAFRTAEFARDRDAIKALIERQDRLIVPMRRGAWLFDFRQSKDNPLGVWLRLPAD
QEPLPDAGWEPVFDLDAFCIRDGKRWNWRGVVTCPWEPTRVLLTLSDGGSDLLRLLEFDAEKKEIVEGGFDTPAARSHAN
WLSRDEICYFGSTDRFSATRSGWPRVGRRLKRGQRPEDAAVMFEAANEDVYGFNLVIDPALSGGSADAGLIDIFVAAHEI
GVASAFLTTADGTRRRIDLPEEADFQFNHDHCLWRAKTDERVATGSLVLQRFDPGSETAPLGPARILFQPGEGQSISQMM
LMREWCVFIISDRLRPRLMVLDLTKPDAEQREIVLPADMQTAHFRPLYADLHLGDDTLCLIGQGFLQPPACYRLELSDRN
KAAEPVFIAAAPSYFDAAGMSSELLEAVSEDGTRVTYRLVLPKHWTKGALPVLLYGYGGFDVSLSPSYSGVTGRWLEQGG
AYVQAYIRGGGEFGPDWYRSAKRQGRDRAFADFVAIARDLVARGYTVPSRIACQGGSNGGLLTGVMLTRYPDDFGAVWCQ
VPVLDMTRFHLFSAGQAWMDEYGDPEVAADRDFMLGYSPLHNVRPATEVTYPPIYIESSANDDRVHPSHARRFAARLEEA
GHRPFFHEFGSGGHGGDGNSEERAARAAMGYSFLRQTIMT

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=261, Percent_Identity=36.7816091954023, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI284172420, Length=224, Percent_Identity=25.8928571428571, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI284172413, Length=224, Percent_Identity=25.8928571428571, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI70778815, Length=224, Percent_Identity=25.8928571428571, Blast_Score=80, Evalue=9e-15,
Organism=Homo sapiens, GI284172438, Length=224, Percent_Identity=25.8928571428571, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI284172431, Length=224, Percent_Identity=25.8928571428571, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI108860686, Length=224, Percent_Identity=25.8928571428571, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI108860692, Length=194, Percent_Identity=25.2577319587629, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788150, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24583414, Length=307, Percent_Identity=36.1563517915309, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI221510989, Length=278, Percent_Identity=34.8920863309352, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 76031; Mature: 76031

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHLETDDDPRTRQFIDDHNRISDAAFRTAEFARDRDAIKALIERQDRLIVPMRRGAWL
CCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
FDFRQSKDNPLGVWLRLPADQEPLPDAGWEPVFDLDAFCIRDGKRWNWRGVVTCPWEPTR
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCEEEEECCCCCCE
VLLTLSDGGSDLLRLLEFDAEKKEIVEGGFDTPAARSHANWLSRDEICYFGSTDRFSATR
EEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCC
SGWPRVGRRLKRGQRPEDAAVMFEAANEDVYGFNLVIDPALSGGSADAGLIDIFVAAHEI
CCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
GVASAFLTTADGTRRRIDLPEEADFQFNHDHCLWRAKTDERVATGSLVLQRFDPGSETAP
HHHHEEEECCCCCCEECCCCCCCCCEECCCEEEEECCCCCCCCHHHEEEEECCCCCCCCC
LGPARILFQPGEGQSISQMMLMREWCVFIISDRLRPRLMVLDLTKPDAEQREIVLPADMQ
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCC
TAHFRPLYADLHLGDDTLCLIGQGFLQPPACYRLELSDRNKAAEPVFIAAAPSYFDAAGM
CCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHCCC
SSELLEAVSEDGTRVTYRLVLPKHWTKGALPVLLYGYGGFDVSLSPSYSGVTGRWLEQGG
HHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHCCC
AYVQAYIRGGGEFGPDWYRSAKRQGRDRAFADFVAIARDLVARGYTVPSRIACQGGSNGG
CEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC
LLTGVMLTRYPDDFGAVWCQVPVLDMTRFHLFSAGQAWMDEYGDPEVAADRDFMLGYSPL
EEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCC
HNVRPATEVTYPPIYIESSANDDRVHPSHARRFAARLEEAGHRPFFHEFGSGGHGGDGNS
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCC
EERAARAAMGYSFLRQTIMT
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHLETDDDPRTRQFIDDHNRISDAAFRTAEFARDRDAIKALIERQDRLIVPMRRGAWL
CCEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
FDFRQSKDNPLGVWLRLPADQEPLPDAGWEPVFDLDAFCIRDGKRWNWRGVVTCPWEPTR
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHEECCCCCCCCEEEEECCCCCCE
VLLTLSDGGSDLLRLLEFDAEKKEIVEGGFDTPAARSHANWLSRDEICYFGSTDRFSATR
EEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCC
SGWPRVGRRLKRGQRPEDAAVMFEAANEDVYGFNLVIDPALSGGSADAGLIDIFVAAHEI
CCCHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHH
GVASAFLTTADGTRRRIDLPEEADFQFNHDHCLWRAKTDERVATGSLVLQRFDPGSETAP
HHHHEEEECCCCCCEECCCCCCCCCEECCCEEEEECCCCCCCCHHHEEEEECCCCCCCCC
LGPARILFQPGEGQSISQMMLMREWCVFIISDRLRPRLMVLDLTKPDAEQREIVLPADMQ
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCC
TAHFRPLYADLHLGDDTLCLIGQGFLQPPACYRLELSDRNKAAEPVFIAAAPSYFDAAGM
CCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHCCC
SSELLEAVSEDGTRVTYRLVLPKHWTKGALPVLLYGYGGFDVSLSPSYSGVTGRWLEQGG
HHHHHHHHHCCCCEEEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHCCC
AYVQAYIRGGGEFGPDWYRSAKRQGRDRAFADFVAIARDLVARGYTVPSRIACQGGSNGG
CEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCC
LLTGVMLTRYPDDFGAVWCQVPVLDMTRFHLFSAGQAWMDEYGDPEVAADRDFMLGYSPL
EEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEEECCCCC
HNVRPATEVTYPPIYIESSANDDRVHPSHARRFAARLEEAGHRPFFHEFGSGGHGGDGNS
CCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCC
EERAARAAMGYSFLRQTIMT
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]