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The map label for this gene is dnaB [H]

Identifier: 209548653

GI number: 209548653

Start: 1068277

End: 1069773

Strand: Reverse

Name: dnaB [H]

Synonym: Rleg2_1050

Alternate gene names: 209548653

Gene position: 1069773-1068277 (Counterclockwise)

Preceding gene: 209548654

Following gene: 209548649

Centisome position: 23.57

GC content: 60.45

Gene sequence:

>1497_bases
ATGAACGACGCCGCTCGAAAGATTGCAGCCGTTGCCCCCTCGGAGCAGCATTATCGCGAAGCACCCAACAATATCGAAGC
CGAGCAGGCGCTTCTGGGTGCCATCCTGATGAACAACGACGCCTATTACCGGGTGTCCGACTTCCTGAAGCCGATCCATC
TTTACGAACCGTTGCACCGGAAGATCTTCGAGGTTGCCGGCGATATCATCCGCATGGGCAAGATCGCCAATCCGGTGACG
ATCAAGACCTTTCTGAAGGCCGACGAGAAGGTCGGCGACATGACGGTGTCGCAATATCTGGCGAGCCTCGTCAGCAACGC
CGTCACCGTCATCAATGCCGAGGACTACGGCCGGGCGATCTACGACCTGGCACTGCGACGGGCGCTGATCACCATCGGCG
AGGATGTCGTCAACATCGCCTATGACGCGCCGCTCGACATGCCGCCGCAGTCGCAGATCGAGGATACCGAACGCCGCCTC
TTCGAACTGGCGGAAAACGGCCGCTACGACGGCGGCTTCCAGGCCTTCAACGATGCGGTTGCGCTGGCGATCGACATGGC
GGCCGTCGCCAAGGAACGCGACGGCGGGCTTTCCGGCATTTCCACCGGCATCCATTCGCTGGATTCCAAGATGGGCGGCC
TGCAGCGTTCGGACTTGATCGTGCTTGCCGGACGCCCCGGCATGGGCAAGACCTCGCTTGCGACCAACATCGCCTACAAT
ATCGCCGCCGCCTACGAAGGCGAAGTCCAGTCGGACGGCAGCATGAAGGCGAAAAACGGCGGCGTCGTCGGCTTCTATTC
GCTCGAAATGTCGTCGGAACAGCTTGCCACCCGTATCATCTCTGAGCAGACGGAAGTCTCGTCCTCGAAGATCCGCCGCG
GCGACATCAACGATGCCGATTTCGAAAAGCTCGTCGCCTGCTCGATGATGATGCAGAAGGTGCCGCTCTATATCGACCAG
ACCGGTGGTATCTCGATCGCCCAGCTTTCGGCGCGCGCGCGCCGTCTCAAGCGTCAGCGCGGCCTCGACGTACTGGTGGT
CGACTACATCCAGCTGATGACCGGCTCGGGAAAATCCAGCGACAACCGCGTGCAGGAAATCACCCAGATCACCACCGGCC
TGAAGGCACTCGGCAAGGAACTCAACGTTCCGATCATCGCGCTGTCGCAGCTCTCGCGCCAGGTCGAAAGCCGCGACGAC
AAGCGGCCGCAGCTTTCCGACCTTCGCGAATCCGGCTCGATCGAGCAGGACGCCGACGTCGTGCTCTTCGTGTTCCGCGA
GGAATATTACGTCAAAAATTCGGAACCGCGCGACATCAACGATCCGAAATATCCGGAATGGGAAGCCTTGTTCGACAAGG
TGAAGGGCACGGCCGATGTCATCATCGCCAAGCAGCGTCACGGGCCGACAGGCACGGTAAAGCTCGCCTTCCAGTCGGAA
TTCACCCGCTTCGCCGATCTCGCCGATCCCTCCTTCACCCATTACGAGGAACATTGA

Upstream 100 bases:

>100_bases
CAGGATTTAAGCGAAGCGGGCGCCTGCTCCTTTCGCAGCCTCTTGATTTTCGCGTCCGGAATTGCAAATCCGGAACTGGA
AAAAGACAGAACGGATGATG

Downstream 100 bases:

>100_bases
CGCGGTTTCCGCGTCAAGTTCCATGGGCGCAATTCCGGGCGCAAAACCGCTGCACACTTTTGCTGAATTGCGCAAAACGC
GTCGCATCAAATTTGATTCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MNDAARKIAAVAPSEQHYREAPNNIEAEQALLGAILMNNDAYYRVSDFLKPIHLYEPLHRKIFEVAGDIIRMGKIANPVT
IKTFLKADEKVGDMTVSQYLASLVSNAVTVINAEDYGRAIYDLALRRALITIGEDVVNIAYDAPLDMPPQSQIEDTERRL
FELAENGRYDGGFQAFNDAVALAIDMAAVAKERDGGLSGISTGIHSLDSKMGGLQRSDLIVLAGRPGMGKTSLATNIAYN
IAAAYEGEVQSDGSMKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDINDADFEKLVACSMMMQKVPLYIDQ
TGGISIAQLSARARRLKRQRGLDVLVVDYIQLMTGSGKSSDNRVQEITQITTGLKALGKELNVPIIALSQLSRQVESRDD
KRPQLSDLRESGSIEQDADVVLFVFREEYYVKNSEPRDINDPKYPEWEALFDKVKGTADVIIAKQRHGPTGTVKLAFQSE
FTRFADLADPSFTHYEEH

Sequences:

>Translated_498_residues
MNDAARKIAAVAPSEQHYREAPNNIEAEQALLGAILMNNDAYYRVSDFLKPIHLYEPLHRKIFEVAGDIIRMGKIANPVT
IKTFLKADEKVGDMTVSQYLASLVSNAVTVINAEDYGRAIYDLALRRALITIGEDVVNIAYDAPLDMPPQSQIEDTERRL
FELAENGRYDGGFQAFNDAVALAIDMAAVAKERDGGLSGISTGIHSLDSKMGGLQRSDLIVLAGRPGMGKTSLATNIAYN
IAAAYEGEVQSDGSMKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDINDADFEKLVACSMMMQKVPLYIDQ
TGGISIAQLSARARRLKRQRGLDVLVVDYIQLMTGSGKSSDNRVQEITQITTGLKALGKELNVPIIALSQLSRQVESRDD
KRPQLSDLRESGSIEQDADVVLFVFREEYYVKNSEPRDINDPKYPEWEALFDKVKGTADVIIAKQRHGPTGTVKLAFQSE
FTRFADLADPSFTHYEEH
>Mature_498_residues
MNDAARKIAAVAPSEQHYREAPNNIEAEQALLGAILMNNDAYYRVSDFLKPIHLYEPLHRKIFEVAGDIIRMGKIANPVT
IKTFLKADEKVGDMTVSQYLASLVSNAVTVINAEDYGRAIYDLALRRALITIGEDVVNIAYDAPLDMPPQSQIEDTERRL
FELAENGRYDGGFQAFNDAVALAIDMAAVAKERDGGLSGISTGIHSLDSKMGGLQRSDLIVLAGRPGMGKTSLATNIAYN
IAAAYEGEVQSDGSMKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDINDADFEKLVACSMMMQKVPLYIDQ
TGGISIAQLSARARRLKRQRGLDVLVVDYIQLMTGSGKSSDNRVQEITQITTGLKALGKELNVPIIALSQLSRQVESRDD
KRPQLSDLRESGSIEQDADVVLFVFREEYYVKNSEPRDINDPKYPEWEALFDKVKGTADVIIAKQRHGPTGTVKLAFQSE
FTRFADLADPSFTHYEEH

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=478, Percent_Identity=34.5188284518828, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54929; Mature: 54929

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAARKIAAVAPSEQHYREAPNNIEAEQALLGAILMNNDAYYRVSDFLKPIHLYEPLHR
CCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
KIFEVAGDIIRMGKIANPVTIKTFLKADEKVGDMTVSQYLASLVSNAVTVINAEDYGRAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
YDLALRRALITIGEDVVNIAYDAPLDMPPQSQIEDTERRLFELAENGRYDGGFQAFNDAV
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ALAIDMAAVAKERDGGLSGISTGIHSLDSKMGGLQRSDLIVLAGRPGMGKTSLATNIAYN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
IAAAYEGEVQSDGSMKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDINDAD
EEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FEKLVACSMMMQKVPLYIDQTGGISIAQLSARARRLKRQRGLDVLVVDYIQLMTGSGKSS
HHHHHHHHHHHHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
DNRVQEITQITTGLKALGKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDADV
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCE
VLFVFREEYYVKNSEPRDINDPKYPEWEALFDKVKGTADVIIAKQRHGPTGTVKLAFQSE
EEEEEECHHEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEHHH
FTRFADLADPSFTHYEEH
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNDAARKIAAVAPSEQHYREAPNNIEAEQALLGAILMNNDAYYRVSDFLKPIHLYEPLHR
CCHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
KIFEVAGDIIRMGKIANPVTIKTFLKADEKVGDMTVSQYLASLVSNAVTVINAEDYGRAI
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHH
YDLALRRALITIGEDVVNIAYDAPLDMPPQSQIEDTERRLFELAENGRYDGGFQAFNDAV
HHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ALAIDMAAVAKERDGGLSGISTGIHSLDSKMGGLQRSDLIVLAGRPGMGKTSLATNIAYN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
IAAAYEGEVQSDGSMKAKNGGVVGFYSLEMSSEQLATRIISEQTEVSSSKIRRGDINDAD
EEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
FEKLVACSMMMQKVPLYIDQTGGISIAQLSARARRLKRQRGLDVLVVDYIQLMTGSGKSS
HHHHHHHHHHHHHCCEEEECCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
DNRVQEITQITTGLKALGKELNVPIIALSQLSRQVESRDDKRPQLSDLRESGSIEQDADV
HHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCE
VLFVFREEYYVKNSEPRDINDPKYPEWEALFDKVKGTADVIIAKQRHGPTGTVKLAFQSE
EEEEEECHHEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCEEEEEEHHH
FTRFADLADPSFTHYEEH
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA