Click here to switch to the map view.

The map label for this gene is iolD1 [H]

Identifier: 209548584

GI number: 209548584

Start: 1000026

End: 1001876

Strand: Reverse

Name: iolD1 [H]

Synonym: Rleg2_0981

Alternate gene names: 209548584

Gene position: 1001876-1000026 (Counterclockwise)

Preceding gene: 209548585

Following gene: 209548583

Centisome position: 22.08

GC content: 62.61

Gene sequence:

>1851_bases
ATGGGCAAGACGATACGATTGACGATGGCGCAGGCCGTCGCGCATTTCCTCAAAAAGCAGATGACTGTTGTCGACGGCAA
GAAGGTGCCGATCTTCGGTGGCGTCTGGGCGATCTTCGGTCATGGCAACGTCGCCGGCATGGGCGAGGCGCTCTACCAGG
TGCGCGGCGAACTGACGACCTATCGCGCCCATAACGAACAGGGCATGGCGCATGCCGCCATCGCCTATGCCAAAGCGAAT
TTCCGCAGCCGCTTCATGGCCTGCACGACCTCGATCGGCCCCGGCGCGCTGAATATGGTGACGGCCGCCGGCGTCGCGCA
TGTCAATCGTATTCCCGTTCTCTTCCTGCCGGGCGACGTCTTTGCCAACCGCGCGCCGGATCCGGTGCTGCAGCAGATCG
AGGATTTCGGCGACGGCACGGTTTCGGCCAATGACGCCTTCCGCCCAGTTTCGCGCTATTTCGACCGCATCACCCGGCCC
GAGCAGATCATTTCGGCGCTGAAGCGCGCCATGCAGGTCCTGACCGATCCGCTGGATTGCGGCCCGGTGACCTTGTCGCT
CTGCCAGGACGTCCAGGCTGAGGCCTATGATTATCCGGAAAGCCTGTTCGATGAAAAAGTATGGACGACCCGCCGGCCGC
AGCCGGATGCGGACGAGCTGGCCAATGTCATCGCGCTGATCAAGGCGTCGCAGAAGCCGGTGATCGTTGCCGGCGGCGGC
GTGCTGTATTCGCAGGCGACGAAGGAGCTTGCTGCTTTTGCCGAGGCTCACGCCATTCCGGTCGTCGTCACCCAGGCCGG
CAAATCGTCGATCAACGAGACCCACCCGCTGGCGCTCGGCTCGGTCGGCGTTACCGGCACGTCGGCGGCGAATGCGATCG
CCGAAGAGACGGATCTCGTCATCGCCGTCGGCACACGCTGCCAGGATTTCACCACCGGCTCTTGGGCGCTGTTCAAGAAC
GACAGCCTCAGGATGGTTGGCCTCAATATCGCCGCTTACGACGCGGTGAAACATGACAGCCATCCGTTGGTCGCCGATGC
CCGCGAAGGGCTGAAGGCGCTTTCATCAGGGCTTTCGGGCTGGAAGACACCAGCGGCACTTGTCGAAAAGGCAACTGCGG
AGAAGAAGGTCTGGATTGAGGCCGCCGCCAAGGCGATGGCGACGACCAATGCCGCCCTGCCCTCCGATGCACAGGTGATC
GGCGCAGTGGCGCGTTCGATCGGCGGCGAGAATACGACGGTGCTTTGCGCGGCCGGCGGTCTTCCCGGCGAATTGCACAA
GCTCTGGCCGGCGACGGCGCCGGGCAGTTATCACATGGAATACGGCTTTTCCTGTATGGGCTACGAGATCGCCGGCGGGC
TCGGCGCCAAGATGGCGCATCCCGAACGTGATGTCGTCGTCATGGTCGGAGACGGTTCCTACATGATGATGAATTCCGAG
ATCGCCACCTCGGTCATGCTCGGCCTCAAGCTCAACATCGTCGTTCTCGACAATCGCGGCTATGGCTGCATCAACCGATT
GCAGATGGGAACCGGCGGCGCCAACTTCAACAACCTGCTCAAGGACTCCTATCACGAGGTGATGCCGGAGATCGATTTCC
GCGCACATGCCGAAAGCATGGGCGCCATCGCCGTCAAGGTCGCCTCGATCGCCGAACTCGAGCAGGCGATCGCCGACTCA
AAGAAGAACGATCGCACCTCGGTCTTCCTCATCGACACCGATCCCTTGATCACCACCGAGGCCGGCGGCCACTGGTGGGA
TGTCGCGGTGCCGGAAGTCAGCCCGCGCGCAGAAGTCAACGAAGCGCGCAAAGGCTATGTCGAAGCGCGCGCTTCCCAGC
GTATCCGCTGA

Upstream 100 bases:

>100_bases
GCGCGGAACGCAGAGAGCCGAAAAGGTTAGTCCCTCCCCCTTGTGGGGAGGGGTTGGGGAGGGGAAAACAGACAAAGTGT
CAAAAGTCCGGGAGGCCCCG

Downstream 100 bases:

>100_bases
TTCGATTGCCATCATTTTCCGGAGGAGCGCCCCACTCCTCCGCACTATTTTTAAGGAGACTATAGATGAAGGCCAAACTC
GGCATGTCGCCCATCGCCTG

Product: thiamine pyrophosphatase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 616; Mature: 615

Protein sequence:

>616_residues
MGKTIRLTMAQAVAHFLKKQMTVVDGKKVPIFGGVWAIFGHGNVAGMGEALYQVRGELTTYRAHNEQGMAHAAIAYAKAN
FRSRFMACTTSIGPGALNMVTAAGVAHVNRIPVLFLPGDVFANRAPDPVLQQIEDFGDGTVSANDAFRPVSRYFDRITRP
EQIISALKRAMQVLTDPLDCGPVTLSLCQDVQAEAYDYPESLFDEKVWTTRRPQPDADELANVIALIKASQKPVIVAGGG
VLYSQATKELAAFAEAHAIPVVVTQAGKSSINETHPLALGSVGVTGTSAANAIAEETDLVIAVGTRCQDFTTGSWALFKN
DSLRMVGLNIAAYDAVKHDSHPLVADAREGLKALSSGLSGWKTPAALVEKATAEKKVWIEAAAKAMATTNAALPSDAQVI
GAVARSIGGENTTVLCAAGGLPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMAHPERDVVVMVGDGSYMMMNSE
IATSVMLGLKLNIVVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVMPEIDFRAHAESMGAIAVKVASIAELEQAIADS
KKNDRTSVFLIDTDPLITTEAGGHWWDVAVPEVSPRAEVNEARKGYVEARASQRIR

Sequences:

>Translated_616_residues
MGKTIRLTMAQAVAHFLKKQMTVVDGKKVPIFGGVWAIFGHGNVAGMGEALYQVRGELTTYRAHNEQGMAHAAIAYAKAN
FRSRFMACTTSIGPGALNMVTAAGVAHVNRIPVLFLPGDVFANRAPDPVLQQIEDFGDGTVSANDAFRPVSRYFDRITRP
EQIISALKRAMQVLTDPLDCGPVTLSLCQDVQAEAYDYPESLFDEKVWTTRRPQPDADELANVIALIKASQKPVIVAGGG
VLYSQATKELAAFAEAHAIPVVVTQAGKSSINETHPLALGSVGVTGTSAANAIAEETDLVIAVGTRCQDFTTGSWALFKN
DSLRMVGLNIAAYDAVKHDSHPLVADAREGLKALSSGLSGWKTPAALVEKATAEKKVWIEAAAKAMATTNAALPSDAQVI
GAVARSIGGENTTVLCAAGGLPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMAHPERDVVVMVGDGSYMMMNSE
IATSVMLGLKLNIVVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVMPEIDFRAHAESMGAIAVKVASIAELEQAIADS
KKNDRTSVFLIDTDPLITTEAGGHWWDVAVPEVSPRAEVNEARKGYVEARASQRIR
>Mature_615_residues
GKTIRLTMAQAVAHFLKKQMTVVDGKKVPIFGGVWAIFGHGNVAGMGEALYQVRGELTTYRAHNEQGMAHAAIAYAKANF
RSRFMACTTSIGPGALNMVTAAGVAHVNRIPVLFLPGDVFANRAPDPVLQQIEDFGDGTVSANDAFRPVSRYFDRITRPE
QIISALKRAMQVLTDPLDCGPVTLSLCQDVQAEAYDYPESLFDEKVWTTRRPQPDADELANVIALIKASQKPVIVAGGGV
LYSQATKELAAFAEAHAIPVVVTQAGKSSINETHPLALGSVGVTGTSAANAIAEETDLVIAVGTRCQDFTTGSWALFKND
SLRMVGLNIAAYDAVKHDSHPLVADAREGLKALSSGLSGWKTPAALVEKATAEKKVWIEAAAKAMATTNAALPSDAQVIG
AVARSIGGENTTVLCAAGGLPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMAHPERDVVVMVGDGSYMMMNSEI
ATSVMLGLKLNIVVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVMPEIDFRAHAESMGAIAVKVASIAELEQAIADSK
KNDRTSVFLIDTDPLITTEAGGHWWDVAVPEVSPRAEVNEARKGYVEARASQRIR

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=26.508226691042, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1790104, Length=518, Percent_Identity=25.4826254826255, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786717, Length=520, Percent_Identity=22.8846153846154, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1787096, Length=479, Percent_Identity=23.3820459290188, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788716, Length=511, Percent_Identity=22.7005870841487, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323755, Length=571, Percent_Identity=23.8178633975482, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323163, Length=556, Percent_Identity=25, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=558, Percent_Identity=24.7311827956989, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321524, Length=399, Percent_Identity=24.812030075188, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 65656; Mature: 65524

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTIRLTMAQAVAHFLKKQMTVVDGKKVPIFGGVWAIFGHGNVAGMGEALYQVRGELTT
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCCCHHHHHHHHHHCCCEE
YRAHNEQGMAHAAIAYAKANFRSRFMACTTSIGPGALNMVTAAGVAHVNRIPVLFLPGDV
EEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHH
FANRAPDPVLQQIEDFGDGTVSANDAFRPVSRYFDRITRPEQIISALKRAMQVLTDPLDC
HCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPVTLSLCQDVQAEAYDYPESLFDEKVWTTRRPQPDADELANVIALIKASQKPVIVAGGG
CCHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
VLYSQATKELAAFAEAHAIPVVVTQAGKSSINETHPLALGSVGVTGTSAANAIAEETDLV
EEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCEE
IAVGTRCQDFTTGSWALFKNDSLRMVGLNIAAYDAVKHDSHPLVADAREGLKALSSGLSG
EEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCC
WKTPAALVEKATAEKKVWIEAAAKAMATTNAALPSDAQVIGAVARSIGGENTTVLCAAGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
LPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMAHPERDVVVMVGDGSYMMMNSE
CCHHHHHHCCCCCCCCEEEECCCEEECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHH
IATSVMLGLKLNIVVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVMPEIDFRAHAESM
HHHHHHCCEEEEEEEECCCCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
GAIAVKVASIAELEQAIADSKKNDRTSVFLIDTDPLITTEAGGHWWDVAVPEVSPRAEVN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCEEEEEECCCCCCCCHHH
EARKGYVEARASQRIR
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKTIRLTMAQAVAHFLKKQMTVVDGKKVPIFGGVWAIFGHGNVAGMGEALYQVRGELTT
CCEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCCCHHHHHHHHHHCCCEE
YRAHNEQGMAHAAIAYAKANFRSRFMACTTSIGPGALNMVTAAGVAHVNRIPVLFLPGDV
EEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHH
FANRAPDPVLQQIEDFGDGTVSANDAFRPVSRYFDRITRPEQIISALKRAMQVLTDPLDC
HCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
GPVTLSLCQDVQAEAYDYPESLFDEKVWTTRRPQPDADELANVIALIKASQKPVIVAGGG
CCHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCC
VLYSQATKELAAFAEAHAIPVVVTQAGKSSINETHPLALGSVGVTGTSAANAIAEETDLV
EEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCEE
IAVGTRCQDFTTGSWALFKNDSLRMVGLNIAAYDAVKHDSHPLVADAREGLKALSSGLSG
EEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHCCCC
WKTPAALVEKATAEKKVWIEAAAKAMATTNAALPSDAQVIGAVARSIGGENTTVLCAAGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCC
LPGELHKLWPATAPGSYHMEYGFSCMGYEIAGGLGAKMAHPERDVVVMVGDGSYMMMNSE
CCHHHHHHCCCCCCCCEEEECCCEEECEEECCCCCCCCCCCCCCEEEEECCCCEEEECHH
IATSVMLGLKLNIVVLDNRGYGCINRLQMGTGGANFNNLLKDSYHEVMPEIDFRAHAESM
HHHHHHCCEEEEEEEECCCCCCHHHHEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHC
GAIAVKVASIAELEQAIADSKKNDRTSVFLIDTDPLITTEAGGHWWDVAVPEVSPRAEVN
CHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEECCCCEEEEEECCCCCCCCHHH
EARKGYVEARASQRIR
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA