Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 209548554

GI number: 209548554

Start: 974334

End: 975686

Strand: Reverse

Name: puuA [H]

Synonym: Rleg2_0951

Alternate gene names: 209548554

Gene position: 975686-974334 (Counterclockwise)

Preceding gene: 209548556

Following gene: 209548552

Centisome position: 21.5

GC content: 66.37

Gene sequence:

>1353_bases
ATGGCGAGCGACAGGATGGACGGCAAGGCCGAGGGCGGCGACCCGCGTTTCGAGATCCTGATCGTCGGCATGAACGGCGA
TCTGCGCGGCAAGCAGCTTCCATCAGGCGCCGAAGGCAAGGTCTGGGCCGGCGAGATCCGCCTGCCGACCTCGACGCAAT
CGCTCGACATCTGGGGCGACGACAATGACGACATTACCGGCCTGTCGCTGACGATCGGCGACCCCGACGGCATGGTCGTT
GCCGACCGGCGCAGCCTGGCGCCAATGCCTTGGGCGCCGGATGGTTCGATGCAGGTGCTTGCCACCATGCACGAATTCGA
TGGCAGCCCGAGCTTCATGGACCCGCGCGCCATCCTGGCTTCGGTGGTCGAGCGCTATCGCGTACGCGGGCTGACGCCTG
TCGTCGCCACCGAACTGGAATTCTACGTGATGTCGGGGGGCTGGCGCGAGACCGGGCGCCCTTCCCCGCCTGAAAGCCTC
ACCTATCGCGGCGAGCCGAACGGTTTCCAGCTCTATGACATGAGCGCCGTCGATGCGCTCGACGGCTATCTCAAGACGCT
GAGAGCCTATGCCAAGGCGCAGGGACTGCCGGCGGATGCGACGACGGCGGAATTCGGCCCCGGCCAGTTCGAGGTCAACC
TGCTGCATCGCCCCGATGCGCTGGCGGCCGCCGACGACTGCCTCTACCTCAAGCGCATCGCTGAACAGGCGGCGCGCCGC
CACGGGCTGAAATCGACCTGCATGGCCAAGCCCTATTCCGACCATGCCGGCTCCGGCCTGCATGTCCATGCAAGCGTCAT
CGACAGCCAAGGCCGCAACATTCTCGATGCCAAGGGCGGCGAACCGACGCGGCTGAAATCGGTGACGGCAGGCCTGCTCG
ACACGATGCGCGCGGCGCAGCTGATCTTCGCCCCCTTCGCAAATTCCTATCGCCGCTTCCAGCCGGGCTCGTTTGCCCCT
GTCGATCTGACCTGGGGCAGCGGCCATCGCGGCACGGCGATCCGCATACCCGACAAGGACGGGCCGGCCGCCCGCATCGA
GCACCGCGTCGCCGGCGCCGATGCCAATCCCTATCTGCTGCTGGCGGCGATCCTCGGCGGCATGCTGCTTGGCCTCGACA
GCGAACTCGACCCCGGCGAGGAGACGACGCCCGCCCACACGCCTGCCGATACGACACGGCTGACGCATGATTTCCTCAGC
GCCGTCGAGACCTTCCGCGCCTCACCCTTCATCGCCGACATCTTCGGCGCACGCTATCAGGCGCTCTATGGCGACACCAA
GCGCAAAGAAGCGCTTGCGCATCTGCGCACCGTCTCGGATTTCGATTACCGCACCTATTTGCCGCGGCTCTGA

Upstream 100 bases:

>100_bases
GGCGGGTAGGTATCGGACATAAGGTGAAGACCCTCGCGGATTGAAAGGCTGCTATCGATGGGATAGGAGCTTGGCCTTGT
CCCGGAGGATTTTCAAGGTC

Downstream 100 bases:

>100_bases
AGCTTACGCCGCGGCCGACTTACGCGGCGGGCTCGCGCCGTTCGGCCTCATCCACCTGCGCCGCCAGCCCCTGCTTGACG
AGAACCTTCAACTCGCCCGG

Product: glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MASDRMDGKAEGGDPRFEILIVGMNGDLRGKQLPSGAEGKVWAGEIRLPTSTQSLDIWGDDNDDITGLSLTIGDPDGMVV
ADRRSLAPMPWAPDGSMQVLATMHEFDGSPSFMDPRAILASVVERYRVRGLTPVVATELEFYVMSGGWRETGRPSPPESL
TYRGEPNGFQLYDMSAVDALDGYLKTLRAYAKAQGLPADATTAEFGPGQFEVNLLHRPDALAAADDCLYLKRIAEQAARR
HGLKSTCMAKPYSDHAGSGLHVHASVIDSQGRNILDAKGGEPTRLKSVTAGLLDTMRAAQLIFAPFANSYRRFQPGSFAP
VDLTWGSGHRGTAIRIPDKDGPAARIEHRVAGADANPYLLLAAILGGMLLGLDSELDPGEETTPAHTPADTTRLTHDFLS
AVETFRASPFIADIFGARYQALYGDTKRKEALAHLRTVSDFDYRTYLPRL

Sequences:

>Translated_450_residues
MASDRMDGKAEGGDPRFEILIVGMNGDLRGKQLPSGAEGKVWAGEIRLPTSTQSLDIWGDDNDDITGLSLTIGDPDGMVV
ADRRSLAPMPWAPDGSMQVLATMHEFDGSPSFMDPRAILASVVERYRVRGLTPVVATELEFYVMSGGWRETGRPSPPESL
TYRGEPNGFQLYDMSAVDALDGYLKTLRAYAKAQGLPADATTAEFGPGQFEVNLLHRPDALAAADDCLYLKRIAEQAARR
HGLKSTCMAKPYSDHAGSGLHVHASVIDSQGRNILDAKGGEPTRLKSVTAGLLDTMRAAQLIFAPFANSYRRFQPGSFAP
VDLTWGSGHRGTAIRIPDKDGPAARIEHRVAGADANPYLLLAAILGGMLLGLDSELDPGEETTPAHTPADTTRLTHDFLS
AVETFRASPFIADIFGARYQALYGDTKRKEALAHLRTVSDFDYRTYLPRL
>Mature_449_residues
ASDRMDGKAEGGDPRFEILIVGMNGDLRGKQLPSGAEGKVWAGEIRLPTSTQSLDIWGDDNDDITGLSLTIGDPDGMVVA
DRRSLAPMPWAPDGSMQVLATMHEFDGSPSFMDPRAILASVVERYRVRGLTPVVATELEFYVMSGGWRETGRPSPPESLT
YRGEPNGFQLYDMSAVDALDGYLKTLRAYAKAQGLPADATTAEFGPGQFEVNLLHRPDALAAADDCLYLKRIAEQAARRH
GLKSTCMAKPYSDHAGSGLHVHASVIDSQGRNILDAKGGEPTRLKSVTAGLLDTMRAAQLIFAPFANSYRRFQPGSFAPV
DLTWGSGHRGTAIRIPDKDGPAARIEHRVAGADANPYLLLAAILGGMLLGLDSELDPGEETTPAHTPADTTRLTHDFLSA
VETFRASPFIADIFGARYQALYGDTKRKEALAHLRTVSDFDYRTYLPRL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=424, Percent_Identity=37.2641509433962, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790301, Length=160, Percent_Identity=35.625, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48763; Mature: 48632

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDRMDGKAEGGDPRFEILIVGMNGDLRGKQLPSGAEGKVWAGEIRLPTSTQSLDIWGD
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
DNDDITGLSLTIGDPDGMVVADRRSLAPMPWAPDGSMQVLATMHEFDGSPSFMDPRAILA
CCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
SVVERYRVRGLTPVVATELEFYVMSGGWRETGRPSPPESLTYRGEPNGFQLYDMSAVDAL
HHHHHHHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEECHHHHHH
DGYLKTLRAYAKAQGLPADATTAEFGPGQFEVNLLHRPDALAAADDCLYLKRIAEQAARR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
HGLKSTCMAKPYSDHAGSGLHVHASVIDSQGRNILDAKGGEPTRLKSVTAGLLDTMRAAQ
CCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
LIFAPFANSYRRFQPGSFAPVDLTWGSGHRGTAIRIPDKDGPAARIEHRVAGADANPYLL
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHH
LAAILGGMLLGLDSELDPGEETTPAHTPADTTRLTHDFLSAVETFRASPFIADIFGARYQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALYGDTKRKEALAHLRTVSDFDYRTYLPRL
HHHCCHHHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure 
ASDRMDGKAEGGDPRFEILIVGMNGDLRGKQLPSGAEGKVWAGEIRLPTSTQSLDIWGD
CCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
DNDDITGLSLTIGDPDGMVVADRRSLAPMPWAPDGSMQVLATMHEFDGSPSFMDPRAILA
CCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
SVVERYRVRGLTPVVATELEFYVMSGGWRETGRPSPPESLTYRGEPNGFQLYDMSAVDAL
HHHHHHHHCCCCHHHCCCEEEEEEECCCCCCCCCCCCCCEEECCCCCCEEEEECHHHHHH
DGYLKTLRAYAKAQGLPADATTAEFGPGQFEVNLLHRPDALAAADDCLYLKRIAEQAARR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
HGLKSTCMAKPYSDHAGSGLHVHASVIDSQGRNILDAKGGEPTRLKSVTAGLLDTMRAAQ
CCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
LIFAPFANSYRRFQPGSFAPVDLTWGSGHRGTAIRIPDKDGPAARIEHRVAGADANPYLL
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCCCHHHHHHHCCCCCCCHHHH
LAAILGGMLLGLDSELDPGEETTPAHTPADTTRLTHDFLSAVETFRASPFIADIFGARYQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ALYGDTKRKEALAHLRTVSDFDYRTYLPRL
HHHCCHHHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]