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The map label for this gene is ccmE

Identifier: 209548524

GI number: 209548524

Start: 934789

End: 935304

Strand: Direct

Name: ccmE

Synonym: Rleg2_0921

Alternate gene names: 209548524

Gene position: 934789-935304 (Clockwise)

Preceding gene: 209548523

Following gene: 209548525

Centisome position: 20.6

GC content: 63.76

Gene sequence:

>516_bases
ATGAACCGCAAGCAGAAGCGTCTGGCGGTGATCGCAGGCGGCATGGGCTTCATTGCCGCCGCCGTGCTGCTGGTCATGTT
CGCCTTCAGCCAGTCGGTCGCCTATTTCTACATGCCGGCCGATCTGGCAAAGACGCCGGTGGCGCCGGAAACCCGCATTC
GGCTTGGCGGCCTGGTCGGCGAGGGCAGCGTCGTGCGCGGCGCCGGCTCGACCGTGGAATTTTCCGTCACCGACGGCAGC
GCCAATGCCGTCAAGGTCAAATATACCGGCATCCTGCCGGATCTCTTCCGCGAAGGGCAGGGGGTCGTCACCGAAGGCAT
GTTTGCACCGGGGACCAACGTCTTTACCGCCGATACCGTGCTTGCCAAGCATGACGAGACCTATATGCCGAAGGACGTGG
CCGACAGGCTGAAGCAGCAGGGGCTGTGGAAGGAAGGCCAGGGCGGCCAGGAGAGCCCAGGGAAAGAGGGTCAAGGCCAG
GAAAATCCGGGCGAGGAGGCGAAAGCGACGCAATGA

Upstream 100 bases:

>100_bases
TGAAGCGCGGGCTTGCCGCCTTTCCGCCGCCCGGCGAACAGGGCAGCCAATTGCTGGCCCTGGCCAAGGAACTCGGCATA
GCCACTGAGGGAGCGACGCA

Downstream 100 bases:

>100_bases
TCATCGAGATCGGCCATTACGCGCTGGTGCTGGCGCTGGCAACGGCGCTCATCCTCTCTGTCGTGCCGGTCATCGGCGCC
CGCCGCCACGACCGGGCGAT

Product: cytochrome C biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MNRKQKRLAVIAGGMGFIAAAVLLVMFAFSQSVAYFYMPADLAKTPVAPETRIRLGGLVGEGSVVRGAGSTVEFSVTDGS
ANAVKVKYTGILPDLFREGQGVVTEGMFAPGTNVFTADTVLAKHDETYMPKDVADRLKQQGLWKEGQGGQESPGKEGQGQ
ENPGEEAKATQ

Sequences:

>Translated_171_residues
MNRKQKRLAVIAGGMGFIAAAVLLVMFAFSQSVAYFYMPADLAKTPVAPETRIRLGGLVGEGSVVRGAGSTVEFSVTDGS
ANAVKVKYTGILPDLFREGQGVVTEGMFAPGTNVFTADTVLAKHDETYMPKDVADRLKQQGLWKEGQGGQESPGKEGQGQ
ENPGEEAKATQ
>Mature_171_residues
MNRKQKRLAVIAGGMGFIAAAVLLVMFAFSQSVAYFYMPADLAKTPVAPETRIRLGGLVGEGSVVRGAGSTVEFSVTDGS
ANAVKVKYTGILPDLFREGQGVVTEGMFAPGTNVFTADTVLAKHDETYMPKDVADRLKQQGLWKEGQGGQESPGKEGQGQ
ENPGEEAKATQ

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family

Homologues:

Organism=Escherichia coli, GI1788525, Length=141, Percent_Identity=41.1347517730496, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCME_RHILW (B5ZVF6)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002280441.1
- ProteinModelPortal:   B5ZVF6
- SMR:   B5ZVF6
- GeneID:   6979639
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_0921
- HOGENOM:   HBG296631
- OMA:   HVEFAVH
- ProtClustDB:   PRK13254
- HAMAP:   MF_01959
- InterPro:   IPR004329
- InterPro:   IPR012340
- Gene3D:   G3DSA:2.40.50.140

Pfam domain/function: PF03100 CcmE; SSF82093 Pyrv/PenolPyrv_Kinase_cat

EC number: NA

Molecular weight: Translated: 18099; Mature: 18099

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 124-124

Signals:

None

Transmembrane regions:

HASH(0x16f2398c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKQKRLAVIAGGMGFIAAAVLLVMFAFSQSVAYFYMPADLAKTPVAPETRIRLGGLVG
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCEEEECEEEC
EGSVVRGAGSTVEFSVTDGSANAVKVKYTGILPDLFREGQGVVTEGMFAPGTNVFTADTV
CCCEEECCCCEEEEEEECCCCCEEEEEECCCCHHHHHCCCCCEECCEECCCCCEEECCCE
LAKHDETYMPKDVADRLKQQGLWKEGQGGQESPGKEGQGQENPGEEAKATQ
EECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
>Mature Secondary Structure
MNRKQKRLAVIAGGMGFIAAAVLLVMFAFSQSVAYFYMPADLAKTPVAPETRIRLGGLVG
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCEEEECEEEC
EGSVVRGAGSTVEFSVTDGSANAVKVKYTGILPDLFREGQGVVTEGMFAPGTNVFTADTV
CCCEEECCCCEEEEEEECCCCCEEEEEECCCCHHHHHCCCCCEECCEECCCCCEEECCCE
LAKHDETYMPKDVADRLKQQGLWKEGQGGQESPGKEGQGQENPGEEAKATQ
EECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA