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The map label for this gene is pbpF [H]

Identifier: 209548497

GI number: 209548497

Start: 909823

End: 912009

Strand: Reverse

Name: pbpF [H]

Synonym: Rleg2_0894

Alternate gene names: 209548497

Gene position: 912009-909823 (Counterclockwise)

Preceding gene: 209548499

Following gene: 209548496

Centisome position: 20.1

GC content: 63.79

Gene sequence:

>2187_bases
GTGCAGGATCCGGACAACCCCGAAAGAGGTCCCGAAGCGGGCCCCAAAGAAACGGCGAATAAGGCGGCGGACAAGCGGCC
GGCGAAGAGCCGCCACATCCTTCTGCGCATCGATTCATGGATCGATTCGACTGTCTGGAACGCCGGCTTCCGCGCCGCCG
AGATCTGGGAAGATATCACCATCTTCTTCCGCCGCTTCCGCGTGCGCGGCTGGAAGCGCATGGTTTTCGAACTGGCCGGC
GAAGGGCTGACGCTTGGGGCGGCCGGCTCGGTGCTGATGCTGCTGCTTGCCCAGCCCGCCTTCGAGGCGACCAAGGAGGA
TTGGCGCAACCGCGGCGATTTCGCCGTCACCTTTACCGATCGCTACGGCAACGTGATCGGCCATCGCGGCGTCATCCACC
AGAACTCCGTGCCGATCGACGAGCTGCCGGATTCCCTGATCAAGTCGGTGCTGGCCACCGAAGACCGCCGCTTCTTCGAC
CATTTCGGCATCGACGTGATCGGCCTCTTCCGTGCTATGGTCACCAACGCCCAGGCCGGCGAGGTTGTGCAGGGCGGCTC
GACGCTGACCCAGCAGCTCGCCAAGAACCTGTTCCTCTCCAACGAGCGCTCGATCGACCGCAAGATCACCGAAGCGTTCC
TCGCCTTGTGGCTGGAGTCGAACCTCTCGAAGAAGGAAATCCTCTCCACCTATCTCGACCGCGCCTATATGGGCGGCGGC
ACGTTTGGCGCAGCCGCGGCGGCGCAGTTCTATTTCGGCAAGAACATCACCGATGTGAACCTGGCCGAATCCGCCATGCT
GGCCGGCCTGTTCAAGGCGCCGGCGAAATATGCGCCGCATGTCAACCTGCCGGCGGCACGTGCTCGCGCCAACGAGGTAC
TGACCAATCTCGTGCAGAGCGGGCTGATGACCGAGGGACAGGTGATCGCCGCCCGGCGCAATCCGGCAACCGTCGTCGAC
CGCAACGAGGTGGAATCGCCCGACTTCTTCCTCGATTGGGCTTTCGACGAGGTGCAGCGGCTTTCGCCGCGCTTCCACCA
GCATTCACTGATCGTGCGCACGACGATCGACATGGGTATCCAGCAGGCGGCCGAGGAATCGGTCGAAACGTCGCTGCGCG
AATATGGCGAAGCCTATCACGCCAAGCAGGGCGCCATGGTGATGATCGAGAACGGCGGCGCGGTGCGCGCCATGGTCGGC
GGGCGGGACTACGGCGAAAGCCAGTTCAACCGCGCCACCAGGGCGCTGCGCCAGCCGGGCTCCTCCTTCAAGGTCTATAC
CTATTCGGTGGCCATGGAGAGCGGGATGACGCCGCAGACAACGATCGTCGACGCACCGATCTCCTGGGGCAACTGGAGCC
CGCACAATTATGCGAACCATTATGCCGGCCGCATAACCCTTGAGACGGCGATCGCCCAATCGATCAACACGGTGCCGGTG
CGGCTCGCCAAAGAGAAGCTCGGCATCCAGCCGATCCGGGCCATGGCGAAGAACCTCGGCGTCGAAACGCCGATCCGCGA
CGACGTCACCATCCCGATCGGTACATCCGAGGTGACGGTGCTCGATCAGGCGACGGCCTATGCCACCTTCCCGGCCGGCG
GCTACCAGTCACGCCGCCACGGCATCACCCAGGTTCTCGATTATGACGGCGACGTGCTCTATGATTTCGACCGCGACGAG
CCGGCGGCCAAACGCGTGCTGTCCGAACAGGCCGACGCCTATATGAACCAGATGCTGTCGCGCGTTCCCTATGTCGGCAC
GGCGCGAAAAGCGGCACTCGACAACGGTATCGTCACGGCCGGCAAGACCGGCACGACGCAGGCCTATCGCGACGCCTGGT
TCGTCGGCTTCACCGGCAACTACACCTGCGCCGTCTGGTTCGGCAATGACGACTATACCTCGACCAACAACATGACCGGC
GGCTCGCTGCCGGCGATGACCTTCAAGCGCGCCATGGACTACGCCCATCAGGGTGTGACGCTGCGCGCCATTCCCGGCAT
CGAAAATCCCCTGCCCTCGGAAAAGGATCTCGCCAAGACCGTCGCCGCCAAGCCGCAGGACGGATTGCCGCAGCTCATCC
GGCCGCGCATGCTCTCGGTGCAATCGACGAATATCCTCAAAGCCCTCGGCGCAAAGCTGAAAAATGCCGCCCCGCTGGCG
CCGGCGAAGGTAGCGAGTGCGGAATAG

Upstream 100 bases:

>100_bases
CTGGTATCGGAGAGCTTTGCGGCCATATGGCGGCCTCGCGCTTTCGCCGCTTTCCGACGGCCGGAGATGAGCGAAACGCG
ATCAGGCGGGAATACAGGAC

Downstream 100 bases:

>100_bases
ATCCTGAGCGGATGCGTCGAACGATGCCGGGAGATCGGCGAGAGTAGCCCCTCATCCGGCTGTCGCCACCTTCTCCCCGC
ACGCGGGGCGAAGGGGCCAA

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 728; Mature: 728

Protein sequence:

>728_residues
MQDPDNPERGPEAGPKETANKAADKRPAKSRHILLRIDSWIDSTVWNAGFRAAEIWEDITIFFRRFRVRGWKRMVFELAG
EGLTLGAAGSVLMLLLAQPAFEATKEDWRNRGDFAVTFTDRYGNVIGHRGVIHQNSVPIDELPDSLIKSVLATEDRRFFD
HFGIDVIGLFRAMVTNAQAGEVVQGGSTLTQQLAKNLFLSNERSIDRKITEAFLALWLESNLSKKEILSTYLDRAYMGGG
TFGAAAAAQFYFGKNITDVNLAESAMLAGLFKAPAKYAPHVNLPAARARANEVLTNLVQSGLMTEGQVIAARRNPATVVD
RNEVESPDFFLDWAFDEVQRLSPRFHQHSLIVRTTIDMGIQQAAEESVETSLREYGEAYHAKQGAMVMIENGGAVRAMVG
GRDYGESQFNRATRALRQPGSSFKVYTYSVAMESGMTPQTTIVDAPISWGNWSPHNYANHYAGRITLETAIAQSINTVPV
RLAKEKLGIQPIRAMAKNLGVETPIRDDVTIPIGTSEVTVLDQATAYATFPAGGYQSRRHGITQVLDYDGDVLYDFDRDE
PAAKRVLSEQADAYMNQMLSRVPYVGTARKAALDNGIVTAGKTGTTQAYRDAWFVGFTGNYTCAVWFGNDDYTSTNNMTG
GSLPAMTFKRAMDYAHQGVTLRAIPGIENPLPSEKDLAKTVAAKPQDGLPQLIRPRMLSVQSTNILKALGAKLKNAAPLA
PAKVASAE

Sequences:

>Translated_728_residues
MQDPDNPERGPEAGPKETANKAADKRPAKSRHILLRIDSWIDSTVWNAGFRAAEIWEDITIFFRRFRVRGWKRMVFELAG
EGLTLGAAGSVLMLLLAQPAFEATKEDWRNRGDFAVTFTDRYGNVIGHRGVIHQNSVPIDELPDSLIKSVLATEDRRFFD
HFGIDVIGLFRAMVTNAQAGEVVQGGSTLTQQLAKNLFLSNERSIDRKITEAFLALWLESNLSKKEILSTYLDRAYMGGG
TFGAAAAAQFYFGKNITDVNLAESAMLAGLFKAPAKYAPHVNLPAARARANEVLTNLVQSGLMTEGQVIAARRNPATVVD
RNEVESPDFFLDWAFDEVQRLSPRFHQHSLIVRTTIDMGIQQAAEESVETSLREYGEAYHAKQGAMVMIENGGAVRAMVG
GRDYGESQFNRATRALRQPGSSFKVYTYSVAMESGMTPQTTIVDAPISWGNWSPHNYANHYAGRITLETAIAQSINTVPV
RLAKEKLGIQPIRAMAKNLGVETPIRDDVTIPIGTSEVTVLDQATAYATFPAGGYQSRRHGITQVLDYDGDVLYDFDRDE
PAAKRVLSEQADAYMNQMLSRVPYVGTARKAALDNGIVTAGKTGTTQAYRDAWFVGFTGNYTCAVWFGNDDYTSTNNMTG
GSLPAMTFKRAMDYAHQGVTLRAIPGIENPLPSEKDLAKTVAAKPQDGLPQLIRPRMLSVQSTNILKALGAKLKNAAPLA
PAKVASAE
>Mature_728_residues
MQDPDNPERGPEAGPKETANKAADKRPAKSRHILLRIDSWIDSTVWNAGFRAAEIWEDITIFFRRFRVRGWKRMVFELAG
EGLTLGAAGSVLMLLLAQPAFEATKEDWRNRGDFAVTFTDRYGNVIGHRGVIHQNSVPIDELPDSLIKSVLATEDRRFFD
HFGIDVIGLFRAMVTNAQAGEVVQGGSTLTQQLAKNLFLSNERSIDRKITEAFLALWLESNLSKKEILSTYLDRAYMGGG
TFGAAAAAQFYFGKNITDVNLAESAMLAGLFKAPAKYAPHVNLPAARARANEVLTNLVQSGLMTEGQVIAARRNPATVVD
RNEVESPDFFLDWAFDEVQRLSPRFHQHSLIVRTTIDMGIQQAAEESVETSLREYGEAYHAKQGAMVMIENGGAVRAMVG
GRDYGESQFNRATRALRQPGSSFKVYTYSVAMESGMTPQTTIVDAPISWGNWSPHNYANHYAGRITLETAIAQSINTVPV
RLAKEKLGIQPIRAMAKNLGVETPIRDDVTIPIGTSEVTVLDQATAYATFPAGGYQSRRHGITQVLDYDGDVLYDFDRDE
PAAKRVLSEQADAYMNQMLSRVPYVGTARKAALDNGIVTAGKTGTTQAYRDAWFVGFTGNYTCAVWFGNDDYTSTNNMTG
GSLPAMTFKRAMDYAHQGVTLRAIPGIENPLPSEKDLAKTVAAKPQDGLPQLIRPRMLSVQSTNILKALGAKLKNAAPLA
PAKVASAE

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=520, Percent_Identity=34.0384615384615, Blast_Score=212, Evalue=8e-56,
Organism=Escherichia coli, GI1788867, Length=529, Percent_Identity=27.9773156899811, Blast_Score=183, Evalue=4e-47,
Organism=Escherichia coli, GI87082258, Length=304, Percent_Identity=35.1973684210526, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789601, Length=173, Percent_Identity=37.5722543352601, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 79904; Mature: 79904

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDPDNPERGPEAGPKETANKAADKRPAKSRHILLRIDSWIDSTVWNAGFRAAEIWEDIT
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHH
IFFRRFRVRGWKRMVFELAGEGLTLGAAGSVLMLLLAQPAFEATKEDWRNRGDFAVTFTD
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEC
RYGNVIGHRGVIHQNSVPIDELPDSLIKSVLATEDRRFFDHFGIDVIGLFRAMVTNAQAG
CCCCHHCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
EVVQGGSTLTQQLAKNLFLSNERSIDRKITEAFLALWLESNLSKKEILSTYLDRAYMGGG
CEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TFGAAAAAQFYFGKNITDVNLAESAMLAGLFKAPAKYAPHVNLPAARARANEVLTNLVQS
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHH
GLMTEGQVIAARRNPATVVDRNEVESPDFFLDWAFDEVQRLSPRFHQHSLIVRTTIDMGI
CCCCCCEEEEECCCCCEEEECCCCCCCCEEEEHHHHHHHHHCCHHHHCCEEEEEHHHHHH
QQAAEESVETSLREYGEAYHAKQGAMVMIENGGAVRAMVGGRDYGESQFNRATRALRQPG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCC
SSFKVYTYSVAMESGMTPQTTIVDAPISWGNWSPHNYANHYAGRITLETAIAQSINTVPV
CCEEEEEEEEEHHCCCCCCCEEEECCCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCHHH
RLAKEKLGIQPIRAMAKNLGVETPIRDDVTIPIGTSEVTVLDQATAYATFPAGGYQSRRH
HHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCEEEECCCCCCCHHHH
GITQVLDYDGDVLYDFDRDEPAAKRVLSEQADAYMNQMLSRVPYVGTARKAALDNGIVTA
CHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEC
GKTGTTQAYRDAWFVGFTGNYTCAVWFGNDDYTSTNNMTGGSLPAMTFKRAMDYAHQGVT
CCCCCCHHHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEE
LRAIPGIENPLPSEKDLAKTVAAKPQDGLPQLIRPRMLSVQSTNILKALGAKLKNAAPLA
EEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCC
PAKVASAE
CCCCCCCC
>Mature Secondary Structure
MQDPDNPERGPEAGPKETANKAADKRPAKSRHILLRIDSWIDSTVWNAGFRAAEIWEDIT
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCHHHHHHHHHH
IFFRRFRVRGWKRMVFELAGEGLTLGAAGSVLMLLLAQPAFEATKEDWRNRGDFAVTFTD
HHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEC
RYGNVIGHRGVIHQNSVPIDELPDSLIKSVLATEDRRFFDHFGIDVIGLFRAMVTNAQAG
CCCCHHCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCC
EVVQGGSTLTQQLAKNLFLSNERSIDRKITEAFLALWLESNLSKKEILSTYLDRAYMGGG
CEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TFGAAAAAQFYFGKNITDVNLAESAMLAGLFKAPAKYAPHVNLPAARARANEVLTNLVQS
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHHHH
GLMTEGQVIAARRNPATVVDRNEVESPDFFLDWAFDEVQRLSPRFHQHSLIVRTTIDMGI
CCCCCCEEEEECCCCCEEEECCCCCCCCEEEEHHHHHHHHHCCHHHHCCEEEEEHHHHHH
QQAAEESVETSLREYGEAYHAKQGAMVMIENGGAVRAMVGGRDYGESQFNRATRALRQPG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCC
SSFKVYTYSVAMESGMTPQTTIVDAPISWGNWSPHNYANHYAGRITLETAIAQSINTVPV
CCEEEEEEEEEHHCCCCCCCEEEECCCCCCCCCCCCCHHHCCCEEEHHHHHHHCCCCHHH
RLAKEKLGIQPIRAMAKNLGVETPIRDDVTIPIGTSEVTVLDQATAYATFPAGGYQSRRH
HHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEECCCEEEECCCCCCCHHHH
GITQVLDYDGDVLYDFDRDEPAAKRVLSEQADAYMNQMLSRVPYVGTARKAALDNGIVTA
CHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEC
GKTGTTQAYRDAWFVGFTGNYTCAVWFGNDDYTSTNNMTGGSLPAMTFKRAMDYAHQGVT
CCCCCCHHHHCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEE
LRAIPGIENPLPSEKDLAKTVAAKPQDGLPQLIRPRMLSVQSTNILKALGAKLKNAAPLA
EEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCC
PAKVASAE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]