Click here to switch to the map view.

The map label for this gene is mrpA [H]

Identifier: 209548477

GI number: 209548477

Start: 892146

End: 894506

Strand: Direct

Name: mrpA [H]

Synonym: Rleg2_0874

Alternate gene names: 209548477

Gene position: 892146-894506 (Clockwise)

Preceding gene: 209548476

Following gene: 209548478

Centisome position: 19.66

GC content: 65.14

Gene sequence:

>2361_bases
ATGGCCGATGTCACGGCTCTGACATTTCTGGCCCTGTGTCTGCCGCTCGCCGGCGCTCTTGCCGCTCCCTTCATCATCCG
CATCTTCGGCGCCAACGGTGCCTGGCTTCTGGCGCTCGCGCCCTTGCTCGCCTTTCTGCATTTCCTGCGTTTCGTGCCGG
CGATCGCGCGCGGCGAGGTCGTCGCCGGCGGTTATGCCTGGGTCCCGAGCTACAATCTCTCCTTCTCCTGGTTCCTCGAC
GGCCTGTCGCTGGCTTTCGCACTGCTGATCACCGGCATCGGCACCCTGATCGTGCTGTATGCCGGCGGCTATCTCAAGGG
ACACAAGGACCAGGGCCGCTTCTTCTCCTTCATCTTCCTCTTCATGGGGGCGATGCTCGGCGTCGTCGTTTCCGACAGTT
TCCTGATGCTCTTCATCTTCTGGGAGCTGACGTCGATCACCTCCTTCCTGCTGATCGGCTTCGACCATGAGCGCGCGGCC
GCACGCCGCGCCGCGCTGCAGGCGCTTGTCGTGACCGGCGGGGGAGGGCTCTGCCTGCTGGCCGGCCTGCTGCTGCTCTG
GAACATCTCGGGCGTCACGCAGCTGTCGAAGCTTATGGTATTTGGCGATGCCGTGCGCGAAAGCCCGCTCTACCTCGCTG
CCCTCATCCTGGTTCTTGCCGGCGCCTTCACCAAGTCGGCGCAGTTCCCCTTTCATTTCTGGCTGCCGAACGCCATGGAG
GCGCCGACGCCGGTGTCGGCCTATCTGCATTCGGCCACCATGGTGAAGGCCGGCGTCTATCTGCTGATGCGGCTCAATCC
CGTCATGGGGGCGACCCCTGCCTGGGAAATCCTTCTGCCCTTCTTCGGCGGGTTGACGCTGGTGGTCGGCGCCACGCTCG
CCATCCGCGAGGCCGACCTGAAGCTTAAGCTCGCCTATACAACCGTCTCCTCGCTCGGCCTGCTCGTAATGCTGATCGGC
TTCGGCTCCGAACATGCGGTGGAGGCGGCAGCGCTCTATCTGGTGGCGCACTGCCTTTTCAAGGGTGCGCTGTTCATGGT
CGCCGGCATCATCGATCACGAGACCGGCACGCGCGATGTCACCAGGCTCGGCGGCCTCGGGCGGGCGATGCCGCTGACAT
GGGTCATCGCCCTTGCGGCGGCCCTCTCCATGGCCGGCCTGCCGCCGTTCTTCGGCTTCCTCGCCAAGGAGGAGATCTAT
ACGGCACTCACCGGCGGCGATATCCGCGCGATAACCTTCACCGCCATCACCGTCTTCGGCAATGCGCTGATGTTTGCCGT
CGCCTTCGCCGTGGCGCTGAAACCCTTTCTCGGCCGACCGGTGGAGACGGAGAAACATCCGCACGAAGCGCCCGTCCTGC
TCTGGCTCGGCCCGGCCGTTCTCGCTACCCTCGGCCTGCTTGCCGCGATCTTTTCGAGCTTCACGCACGCTGCCCTGTCG
TCGCCGATATCAGCCGCCATCCGCCAGACCCCTGCTGAGATCGACATTTCGCTGGCGCTGCATCCAGGCGTGCCCTTGGC
GCTCTCCGCTTTGACGGTGCTCCTCGGCATCGGCGTCTATTGGCAGCTCGCCAGCGTCCGCGCGGCGATGGCCATCTTCC
TGCGCGCCGCCGGCCGCGGGCCCGATCACGGTTTCGACCTTGCCATATCGGGCCTTGTCCGCTTCGCCGCCCGCACCATG
CGGATTCTCCAGCCGGGGCGGCTGCAGATCTATATCGCCTGCACCTTCCTCTCGGTCGCCGCCATCCTCATCATTCCCCC
CATCGTCTATGGTGAACTGCCACACCTTCCCGCCTGGCCGGTCGATGTCAGGCTCTATGAATGGGCGGTTTTGCTGATCG
CCGCCGTCGGCCTTGCCGCCGTGCTCGTCGCCCACGACCGGCTGACGGCGATCGTCTCGCTCGGCATTCAGGGTTTTGCC
GTCGCCGTCATCTTCCTGCTGTTCGGGGCGCCGGATCTCTCCTTCACCCAGTTCATGGTCGAAACCCTGTCGGTGGTCAT
CCTCGCCCTTGTGATGACCAGGCTCCGCCTCTCGCCGGACGATCGGCGCCCGCTCGGCCGCCGGCTCTTCGACGGTACGC
TTGCGCTTGTCTGCGGCCTCGGCTTCACGCTTCTGCTGATGCGGGCAACGCAGGTGCCGTTCAACGATGCGCTGACGGTG
TTCTTCAACGCCTATTCGAGAACGATTGCCCATGGCGCCAATGTCGTCAACGTCATCATCGTCGACTTCCGCGGCACCGA
CACGCTCGGCGAAATCGCCGTCGTCGCGGTCACCGGCCTCGCCATCCTGGCGCTGATCCGCATCCGCGCCGGCACCGAGC
GCAAGCTTGCCGCCAATGATCCAGTGGAGGCCGAGGGCTGA

Upstream 100 bases:

>100_bases
CCCTCCGATCGAAATTGCTTGAGGCGCTCTACCATTTCGCCTAAGTCAGAAGGCAGTCGGTTTTCTCCGGCTTTCGTCTG
TTGCCGGTAGAGGAGAAGAC

Downstream 100 bases:

>100_bases
TGCATACCTTGATCTTCCGCACCGTCGCCCCGTTCCTCACCGCGCTGATGCTGCTTTTTTCCGTCTTCGTGCTGCTGCGC
GGCCATAACGAGCCGGGCGG

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 786; Mature: 785

Protein sequence:

>786_residues
MADVTALTFLALCLPLAGALAAPFIIRIFGANGAWLLALAPLLAFLHFLRFVPAIARGEVVAGGYAWVPSYNLSFSWFLD
GLSLAFALLITGIGTLIVLYAGGYLKGHKDQGRFFSFIFLFMGAMLGVVVSDSFLMLFIFWELTSITSFLLIGFDHERAA
ARRAALQALVVTGGGGLCLLAGLLLLWNISGVTQLSKLMVFGDAVRESPLYLAALILVLAGAFTKSAQFPFHFWLPNAME
APTPVSAYLHSATMVKAGVYLLMRLNPVMGATPAWEILLPFFGGLTLVVGATLAIREADLKLKLAYTTVSSLGLLVMLIG
FGSEHAVEAAALYLVAHCLFKGALFMVAGIIDHETGTRDVTRLGGLGRAMPLTWVIALAAALSMAGLPPFFGFLAKEEIY
TALTGGDIRAITFTAITVFGNALMFAVAFAVALKPFLGRPVETEKHPHEAPVLLWLGPAVLATLGLLAAIFSSFTHAALS
SPISAAIRQTPAEIDISLALHPGVPLALSALTVLLGIGVYWQLASVRAAMAIFLRAAGRGPDHGFDLAISGLVRFAARTM
RILQPGRLQIYIACTFLSVAAILIIPPIVYGELPHLPAWPVDVRLYEWAVLLIAAVGLAAVLVAHDRLTAIVSLGIQGFA
VAVIFLLFGAPDLSFTQFMVETLSVVILALVMTRLRLSPDDRRPLGRRLFDGTLALVCGLGFTLLLMRATQVPFNDALTV
FFNAYSRTIAHGANVVNVIIVDFRGTDTLGEIAVVAVTGLAILALIRIRAGTERKLAANDPVEAEG

Sequences:

>Translated_786_residues
MADVTALTFLALCLPLAGALAAPFIIRIFGANGAWLLALAPLLAFLHFLRFVPAIARGEVVAGGYAWVPSYNLSFSWFLD
GLSLAFALLITGIGTLIVLYAGGYLKGHKDQGRFFSFIFLFMGAMLGVVVSDSFLMLFIFWELTSITSFLLIGFDHERAA
ARRAALQALVVTGGGGLCLLAGLLLLWNISGVTQLSKLMVFGDAVRESPLYLAALILVLAGAFTKSAQFPFHFWLPNAME
APTPVSAYLHSATMVKAGVYLLMRLNPVMGATPAWEILLPFFGGLTLVVGATLAIREADLKLKLAYTTVSSLGLLVMLIG
FGSEHAVEAAALYLVAHCLFKGALFMVAGIIDHETGTRDVTRLGGLGRAMPLTWVIALAAALSMAGLPPFFGFLAKEEIY
TALTGGDIRAITFTAITVFGNALMFAVAFAVALKPFLGRPVETEKHPHEAPVLLWLGPAVLATLGLLAAIFSSFTHAALS
SPISAAIRQTPAEIDISLALHPGVPLALSALTVLLGIGVYWQLASVRAAMAIFLRAAGRGPDHGFDLAISGLVRFAARTM
RILQPGRLQIYIACTFLSVAAILIIPPIVYGELPHLPAWPVDVRLYEWAVLLIAAVGLAAVLVAHDRLTAIVSLGIQGFA
VAVIFLLFGAPDLSFTQFMVETLSVVILALVMTRLRLSPDDRRPLGRRLFDGTLALVCGLGFTLLLMRATQVPFNDALTV
FFNAYSRTIAHGANVVNVIIVDFRGTDTLGEIAVVAVTGLAILALIRIRAGTERKLAANDPVEAEG
>Mature_785_residues
ADVTALTFLALCLPLAGALAAPFIIRIFGANGAWLLALAPLLAFLHFLRFVPAIARGEVVAGGYAWVPSYNLSFSWFLDG
LSLAFALLITGIGTLIVLYAGGYLKGHKDQGRFFSFIFLFMGAMLGVVVSDSFLMLFIFWELTSITSFLLIGFDHERAAA
RRAALQALVVTGGGGLCLLAGLLLLWNISGVTQLSKLMVFGDAVRESPLYLAALILVLAGAFTKSAQFPFHFWLPNAMEA
PTPVSAYLHSATMVKAGVYLLMRLNPVMGATPAWEILLPFFGGLTLVVGATLAIREADLKLKLAYTTVSSLGLLVMLIGF
GSEHAVEAAALYLVAHCLFKGALFMVAGIIDHETGTRDVTRLGGLGRAMPLTWVIALAAALSMAGLPPFFGFLAKEEIYT
ALTGGDIRAITFTAITVFGNALMFAVAFAVALKPFLGRPVETEKHPHEAPVLLWLGPAVLATLGLLAAIFSSFTHAALSS
PISAAIRQTPAEIDISLALHPGVPLALSALTVLLGIGVYWQLASVRAAMAIFLRAAGRGPDHGFDLAISGLVRFAARTMR
ILQPGRLQIYIACTFLSVAAILIIPPIVYGELPHLPAWPVDVRLYEWAVLLIAAVGLAAVLVAHDRLTAIVSLGIQGFAV
AVIFLLFGAPDLSFTQFMVETLSVVILALVMTRLRLSPDDRRPLGRRLFDGTLALVCGLGFTLLLMRATQVPFNDALTVF
FNAYSRTIAHGANVVNVIIVDFRGTDTLGEIAVVAVTGLAILALIRIRAGTERKLAANDPVEAEG

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=388, Percent_Identity=33.7628865979381, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1788614, Length=432, Percent_Identity=32.1759259259259, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1788829, Length=348, Percent_Identity=35.0574712643678, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788831, Length=342, Percent_Identity=30.9941520467836, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788613, Length=398, Percent_Identity=27.3869346733668, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788827, Length=434, Percent_Identity=26.2672811059908, Blast_Score=109, Evalue=7e-25,
Organism=Escherichia coli, GI145693160, Length=380, Percent_Identity=26.8421052631579, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI2367154, Length=289, Percent_Identity=29.0657439446367, Blast_Score=83, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 83732; Mature: 83601

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVTALTFLALCLPLAGALAAPFIIRIFGANGAWLLALAPLLAFLHFLRFVPAIARGEV
CCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VAGGYAWVPSYNLSFSWFLDGLSLAFALLITGIGTLIVLYAGGYLKGHKDQGRFFSFIFL
EECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FMGAMLGVVVSDSFLMLFIFWELTSITSFLLIGFDHERAAARRAALQALVVTGGGGLCLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCHHHHH
AGLLLLWNISGVTQLSKLMVFGDAVRESPLYLAALILVLAGAFTKSAQFPFHFWLPNAME
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
APTPVSAYLHSATMVKAGVYLLMRLNPVMGATPAWEILLPFFGGLTLVVGATLAIREADL
CCCCHHHHHHHHHHHHHHHHHHHEECHHCCCCCHHHHHHHHHCHHHHHHHHHHEEEECCE
KLKLAYTTVSSLGLLVMLIGFGSEHAVEAAALYLVAHCLFKGALFMVAGIIDHETGTRDV
EEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRLGGLGRAMPLTWVIALAAALSMAGLPPFFGFLAKEEIYTALTGGDIRAITFTAITVFG
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
NALMFAVAFAVALKPFLGRPVETEKHPHEAPVLLWLGPAVLATLGLLAAIFSSFTHAALS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHC
SPISAAIRQTPAEIDISLALHPGVPLALSALTVLLGIGVYWQLASVRAAMAIFLRAAGRG
CHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDHGFDLAISGLVRFAARTMRILQPGRLQIYIACTFLSVAAILIIPPIVYGELPHLPAWP
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCHHHHCCCCCCCCCC
VDVRLYEWAVLLIAAVGLAAVLVAHDRLTAIVSLGIQGFAVAVIFLLFGAPDLSFTQFMV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHH
ETLSVVILALVMTRLRLSPDDRRPLGRRLFDGTLALVCGLGFTLLLMRATQVPFNDALTV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FFNAYSRTIAHGANVVNVIIVDFRGTDTLGEIAVVAVTGLAILALIRIRAGTERKLAAND
HHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PVEAEG
CCCCCC
>Mature Secondary Structure 
ADVTALTFLALCLPLAGALAAPFIIRIFGANGAWLLALAPLLAFLHFLRFVPAIARGEV
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
VAGGYAWVPSYNLSFSWFLDGLSLAFALLITGIGTLIVLYAGGYLKGHKDQGRFFSFIFL
EECCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
FMGAMLGVVVSDSFLMLFIFWELTSITSFLLIGFDHERAAARRAALQALVVTGGGGLCLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEECCCHHHHH
AGLLLLWNISGVTQLSKLMVFGDAVRESPLYLAALILVLAGAFTKSAQFPFHFWLPNAME
HHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
APTPVSAYLHSATMVKAGVYLLMRLNPVMGATPAWEILLPFFGGLTLVVGATLAIREADL
CCCCHHHHHHHHHHHHHHHHHHHEECHHCCCCCHHHHHHHHHCHHHHHHHHHHEEEECCE
KLKLAYTTVSSLGLLVMLIGFGSEHAVEAAALYLVAHCLFKGALFMVAGIIDHETGTRDV
EEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TRLGGLGRAMPLTWVIALAAALSMAGLPPFFGFLAKEEIYTALTGGDIRAITFTAITVFG
HHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHH
NALMFAVAFAVALKPFLGRPVETEKHPHEAPVLLWLGPAVLATLGLLAAIFSSFTHAALS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHC
SPISAAIRQTPAEIDISLALHPGVPLALSALTVLLGIGVYWQLASVRAAMAIFLRAAGRG
CHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PDHGFDLAISGLVRFAARTMRILQPGRLQIYIACTFLSVAAILIIPPIVYGELPHLPAWP
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCHHHHCCCCCCCCCC
VDVRLYEWAVLLIAAVGLAAVLVAHDRLTAIVSLGIQGFAVAVIFLLFGAPDLSFTQFMV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHH
ETLSVVILALVMTRLRLSPDDRRPLGRRLFDGTLALVCGLGFTLLLMRATQVPFNDALTV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FFNAYSRTIAHGANVVNVIIVDFRGTDTLGEIAVVAVTGLAILALIRIRAGTERKLAAND
HHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PVEAEG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]