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The map label for this gene is hsrA [H]

Identifier: 209548461

GI number: 209548461

Start: 876534

End: 877976

Strand: Direct

Name: hsrA [H]

Synonym: Rleg2_0858

Alternate gene names: 209548461

Gene position: 876534-877976 (Clockwise)

Preceding gene: 209548458

Following gene: 209548464

Centisome position: 19.32

GC content: 61.82

Gene sequence:

>1443_bases
ATGTCGGATCAGATTTACCAGGCGCCGATGGTTCGGCGCGCCGCACCCAATTTCCGGGTGATCGCGATGATCGTCGCGAG
TGCGATGCTGATGGAAAACATCGACGCAACCGTGCTGGCGACGGCGCTGCCCACCATGGCGCGCGATTTTAACGTCAGCG
CCCCGGCCATGTCGATCGCGCTGACCTCCTATCTCCTCAGTCTGGCGATCTTCATTCCGGCGAGCGGCCGGATGGCCGAC
AGTTTTGGCTCCCGCACTGTCTTCCGCTCGGCGATCGCCGTCTTCGTCGTCGGCTCGATCCTCTGCGCGCTGGCGCCGAC
CCTGTCGTTCCTGGTGTTGGCACGGCTGCTTCAGGGCATAGGCGGTGCGATGATGATGCCGGTCGGACGCCTGGTGCTGA
TGCGCAGCGTCGCCCGCAAGGATATGGTCAGCGCCATGTCCTGGCTGCTGGTGCCGGCGCTGATCGGCCCGATCGTCGGC
CCGCCGCTCGGCGGCTTCTTCGTCACCTATCTCGACTGGCGCTGGATCTTTTACATCAACGTGCCGGTCGGCATCATCGG
CATGATCTTCGTCTCGATCTATATCGACGAGGTCAAGGGCAAGGCGAGCGGTCCCTTCGATACGATCGGCTTCATTCTAT
CAGGCCTTTCGCTCGGCTCGCTGCTTTTTGGCTTCGAAATGTCGAGCCATGAAGGCGAGGGCGCCTTCTCGATCTTCCTG
ATCGCTGTCGGCCTCATCTTCGGCATCGGCTATCTCAGACATGCCCGCAGGCATCCGTCGCCGATCATGGATTTTTCGCT
GATGAAGGTGCCGAGCTTCGGCACGTCCGTCATCGCCGGTTCGCTGACACGCATCACCCAGGGTGCCCAGCCCTTCCTGC
TGCCGCTGCTTTTCCAGATCGGCTTCGGCCTGTCGGCGGCTGCAGCCGGCCAGATCGTCATTGCCACGGCACTTGGCGCG
CTCGCCATGAAGCCGATGGCGAAGTTCGTCTTCCGCCGGCTCGGTTTCCGCCGCAGCCTCGTCCTTAACGGCATTCTCGG
CACGATCGGCTATGGCCTCTGCGCCGCCTTCCGGCCGGACTGGCCGATGCCGCTGATCTTCATCGTCCTGGTCCTCAGCG
CCTTCTTCCTGTCCTTCCAGTTCACCGCCTATAACACCATCGCCTATGACGAGATCGACAAAGAACGGATGAGCTCGGCC
ACGAGCTTCTACACCACCTTCCAGCAGCTCATGCTGTCGCTCGGCATCTGCATCGGCGCCTTGGCGCTGCACGGCTCGAT
GGCTTTCAACAATGTCGAAACACCGTCGCTTGGCGATTTCTCCATAGCCTTCATCGTCGTCACCATCATCTCGATCACCG
CCACCATCTGGAACCTGCGCTTCTCCAAAACCGCCGGCGAAGAGATCAGCGGCTACAAGGCCAAGCAGACGGCAAGGGGC
TGA

Upstream 100 bases:

>100_bases
GCGTATAGAAAATCGTCTCCCCTTGGCGTTACGCCATGTCATCCCCCGCGGCCCTGCAGCCTCGTGTGCTCCGCCGCGCC
ACCGTGAGTGATCCCGGACG

Downstream 100 bases:

>100_bases
TGTTGTATAAAAACAATGGCTTGATATCCCGCGCTTGAATCAAATTCTGCGTTGGACTCTCAGCCTTCCATCGCGGCCGC
CATGTCCGCGAGCTTGCGCA

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSDQIYQAPMVRRAAPNFRVIAMIVASAMLMENIDATVLATALPTMARDFNVSAPAMSIALTSYLLSLAIFIPASGRMAD
SFGSRTVFRSAIAVFVVGSILCALAPTLSFLVLARLLQGIGGAMMMPVGRLVLMRSVARKDMVSAMSWLLVPALIGPIVG
PPLGGFFVTYLDWRWIFYINVPVGIIGMIFVSIYIDEVKGKASGPFDTIGFILSGLSLGSLLFGFEMSSHEGEGAFSIFL
IAVGLIFGIGYLRHARRHPSPIMDFSLMKVPSFGTSVIAGSLTRITQGAQPFLLPLLFQIGFGLSAAAAGQIVIATALGA
LAMKPMAKFVFRRLGFRRSLVLNGILGTIGYGLCAAFRPDWPMPLIFIVLVLSAFFLSFQFTAYNTIAYDEIDKERMSSA
TSFYTTFQQLMLSLGICIGALALHGSMAFNNVETPSLGDFSIAFIVVTIISITATIWNLRFSKTAGEEISGYKAKQTARG

Sequences:

>Translated_480_residues
MSDQIYQAPMVRRAAPNFRVIAMIVASAMLMENIDATVLATALPTMARDFNVSAPAMSIALTSYLLSLAIFIPASGRMAD
SFGSRTVFRSAIAVFVVGSILCALAPTLSFLVLARLLQGIGGAMMMPVGRLVLMRSVARKDMVSAMSWLLVPALIGPIVG
PPLGGFFVTYLDWRWIFYINVPVGIIGMIFVSIYIDEVKGKASGPFDTIGFILSGLSLGSLLFGFEMSSHEGEGAFSIFL
IAVGLIFGIGYLRHARRHPSPIMDFSLMKVPSFGTSVIAGSLTRITQGAQPFLLPLLFQIGFGLSAAAAGQIVIATALGA
LAMKPMAKFVFRRLGFRRSLVLNGILGTIGYGLCAAFRPDWPMPLIFIVLVLSAFFLSFQFTAYNTIAYDEIDKERMSSA
TSFYTTFQQLMLSLGICIGALALHGSMAFNNVETPSLGDFSIAFIVVTIISITATIWNLRFSKTAGEEISGYKAKQTARG
>Mature_479_residues
SDQIYQAPMVRRAAPNFRVIAMIVASAMLMENIDATVLATALPTMARDFNVSAPAMSIALTSYLLSLAIFIPASGRMADS
FGSRTVFRSAIAVFVVGSILCALAPTLSFLVLARLLQGIGGAMMMPVGRLVLMRSVARKDMVSAMSWLLVPALIGPIVGP
PLGGFFVTYLDWRWIFYINVPVGIIGMIFVSIYIDEVKGKASGPFDTIGFILSGLSLGSLLFGFEMSSHEGEGAFSIFLI
AVGLIFGIGYLRHARRHPSPIMDFSLMKVPSFGTSVIAGSLTRITQGAQPFLLPLLFQIGFGLSAAAAGQIVIATALGAL
AMKPMAKFVFRRLGFRRSLVLNGILGTIGYGLCAAFRPDWPMPLIFIVLVLSAFFLSFQFTAYNTIAYDEIDKERMSSAT
SFYTTFQQLMLSLGICIGALALHGSMAFNNVETPSLGDFSIAFIVVTIISITATIWNLRFSKTAGEEISGYKAKQTARG

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=460, Percent_Identity=38.2608695652174, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=36.6336633663366, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081983, Length=411, Percent_Identity=27.4939172749392, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788710, Length=400, Percent_Identity=26.25, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=24.4444444444444, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790146, Length=165, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788509, Length=171, Percent_Identity=32.1637426900585, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=183, Percent_Identity=32.2404371584699, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=30.0653594771242, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323735, Length=159, Percent_Identity=29.559748427673, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51695; Mature: 51564

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIYQAPMVRRAAPNFRVIAMIVASAMLMENIDATVLATALPTMARDFNVSAPAMSIA
CCCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH
LTSYLLSLAIFIPASGRMADSFGSRTVFRSAIAVFVVGSILCALAPTLSFLVLARLLQGI
HHHHHHHHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGAMMMPVGRLVLMRSVARKDMVSAMSWLLVPALIGPIVGPPLGGFFVTYLDWRWIFYIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEEEEE
VPVGIIGMIFVSIYIDEVKGKASGPFDTIGFILSGLSLGSLLFGFEMSSHEGEGAFSIFL
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IAVGLIFGIGYLRHARRHPSPIMDFSLMKVPSFGTSVIAGSLTRITQGAQPFLLPLLFQI
HHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
GFGLSAAAAGQIVIATALGALAMKPMAKFVFRRLGFRRSLVLNGILGTIGYGLCAAFRPD
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCC
WPMPLIFIVLVLSAFFLSFQFTAYNTIAYDEIDKERMSSATSFYTTFQQLMLSLGICIGA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALHGSMAFNNVETPSLGDFSIAFIVVTIISITATIWNLRFSKTAGEEISGYKAKQTARG
HHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCHHCCCCCHHCCCC
>Mature Secondary Structure 
SDQIYQAPMVRRAAPNFRVIAMIVASAMLMENIDATVLATALPTMARDFNVSAPAMSIA
CCCHHHCHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]