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The map label for this gene is lig [H]

Identifier: 209548406

GI number: 209548406

Start: 822804

End: 824429

Strand: Direct

Name: lig [H]

Synonym: Rleg2_0803

Alternate gene names: 209548406

Gene position: 822804-824429 (Clockwise)

Preceding gene: 209548405

Following gene: 209548407

Centisome position: 18.13

GC content: 63.71

Gene sequence:

>1626_bases
ATGAAAGCCTTCGCCGACCTCCTCGACCGCCTCGTCCTCACCCCCAGCCGCAACGGCAAGCTGAAGCTGCTTACCGATTA
CTTCCGCGACACGCCGGACCCTGACCGCGGCTACGGCCTTGCCGCCATCGCCGGCACGCTTGAAGTGCGCAACGTCAAGC
CCGCCATGCTGCGCGAATTGGTGCTGGAGCGCATGGACGAGGTGTTGTTCCGTTATTCCTACGATTATGTCGGCGATCTC
GCCGAAACGATCTCGCTCGTCTGGGACAATGAGCGGGATATCGACCGCTCGGCCCTTTCCCAGCCGCGTCTCGGGACTGT
TGTCACCGATATGAATGCGCTCGGCCGCACCGAAGTGCGCGGCTTCGTCCGCGACCTGCTCGATCGGCTGGATTCCTCCG
GCCGGTTCGCTTTCATCAAGCTGGCGACCGGCGCCATGCGCATCGGCGTTTCCGCCCGTCTTGCCAAACAGGCGCTGGCC
GATCTCGGCGATAAGGACGTCACCGAAATCGAAACGCTCTGGCACGGCCTGCAGCCGCCTTACGAGACATTGTTCGAATG
GCTGGCGGCGGGCGGCGAAAAACCGGTGCTGGCGACACCGGCGATCTTCCATTCGGTCATGCTTGCCAATGCGGTGGAGG
AGGGGGATCTTAACGGTCTCGATCCGGCCAGCTACGCCGCCGAATGGAAATGGGACGGCATCCGTGTCCAGCTCTCCCGC
TCCGGCGATCGGCGCAAGATCTATTCCCGCTCCGGCGACGATATTTCGGGCGCCTTTCCCGATATTCTCGACGCCATCAG
TTTTTCCGGCGTCGTCGACGGCGAGCTGCTGGTCGGCGGCACCGCCCGCTCCAACAGCCCGACCCGCACCTTCTCCGACC
TGCAGCAGCGCCTCAATCGCAAGACGGTGACGGCAAAGATGCTTTCCGACTACCCCGCCTTCATCCGCACCTACGACCTG
CTCTTCGACGATGACGAGGATGTGCGGGGCCGTGGCTATACCGATCGGCGCGATCGGCTGGCCGAGATCATCGACCGCGC
TCCCCACGACCGTTTCGACCTGTCGCCGCTCGTGCCTTTCTCGTCCTGGGACGAGCTCGACCGTCTCCGCGCCGCCCCGC
CCGATCCGGTCATCGAGGGCGTGATGATCAAGCGGCGCGACAGCATCTACCAGGCCGGCCGGATGAAGGGCCCCTGGTTC
AAGTGGAAGCGCAATCCCTATAATGTCGATGCGGTGCTGATGTATGCGCAGCGCGGCCATGGCAAGCGCTCCAGCTACTA
TTCCGATTTCACCTTCGGCGTCTGGGCCGACGATGAGGACGGTGAACAGCTGGTGCCCGTCGGCAAGGCCTATTTCGGAT
TCACCGATGCCGAGCTCGAAGTGCTCGACAAGTTCGTGCGCAACAATACCACCGAGCGTTTCGGTCCGGTGCGCGCCGTG
CGCGCCGACAAGGATTTCGGCTTCGTCGTCGAGGTGGCCTTTGAAGGCATCAACCGCTCGACCCGGCACAAATCGGGCGT
CGCCATGCGCTTTCCCCGCATCGCTCGTCTTCGCCCCGACAAGCCCACTTATGAGGCCGACCGGCTGCGCACGCTGATCG
CCATGATCGACGCCAAGCCGGGGTGA

Upstream 100 bases:

>100_bases
AAGACCCCTCCAGAGCCGACCTTGCCGCCACTTTGGATGTCGCAGCGACGCCTGTCCGACACTCCCTCATTGAGCCTTCT
TCCCAAACGCGGAGGCCGCT

Downstream 100 bases:

>100_bases
GGGCCAATCTCCTGTGCAACAATGCCGCAGTCCCAAAATTTCAACAGGATGTGTATTGGCGGCAACGATCTTCCGGTGCA
AATCTTCTGTGCCGGGGAAG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKAFADLLDRLVLTPSRNGKLKLLTDYFRDTPDPDRGYGLAAIAGTLEVRNVKPAMLRELVLERMDEVLFRYSYDYVGDL
AETISLVWDNERDIDRSALSQPRLGTVVTDMNALGRTEVRGFVRDLLDRLDSSGRFAFIKLATGAMRIGVSARLAKQALA
DLGDKDVTEIETLWHGLQPPYETLFEWLAAGGEKPVLATPAIFHSVMLANAVEEGDLNGLDPASYAAEWKWDGIRVQLSR
SGDRRKIYSRSGDDISGAFPDILDAISFSGVVDGELLVGGTARSNSPTRTFSDLQQRLNRKTVTAKMLSDYPAFIRTYDL
LFDDDEDVRGRGYTDRRDRLAEIIDRAPHDRFDLSPLVPFSSWDELDRLRAAPPDPVIEGVMIKRRDSIYQAGRMKGPWF
KWKRNPYNVDAVLMYAQRGHGKRSSYYSDFTFGVWADDEDGEQLVPVGKAYFGFTDAELEVLDKFVRNNTTERFGPVRAV
RADKDFGFVVEVAFEGINRSTRHKSGVAMRFPRIARLRPDKPTYEADRLRTLIAMIDAKPG

Sequences:

>Translated_541_residues
MKAFADLLDRLVLTPSRNGKLKLLTDYFRDTPDPDRGYGLAAIAGTLEVRNVKPAMLRELVLERMDEVLFRYSYDYVGDL
AETISLVWDNERDIDRSALSQPRLGTVVTDMNALGRTEVRGFVRDLLDRLDSSGRFAFIKLATGAMRIGVSARLAKQALA
DLGDKDVTEIETLWHGLQPPYETLFEWLAAGGEKPVLATPAIFHSVMLANAVEEGDLNGLDPASYAAEWKWDGIRVQLSR
SGDRRKIYSRSGDDISGAFPDILDAISFSGVVDGELLVGGTARSNSPTRTFSDLQQRLNRKTVTAKMLSDYPAFIRTYDL
LFDDDEDVRGRGYTDRRDRLAEIIDRAPHDRFDLSPLVPFSSWDELDRLRAAPPDPVIEGVMIKRRDSIYQAGRMKGPWF
KWKRNPYNVDAVLMYAQRGHGKRSSYYSDFTFGVWADDEDGEQLVPVGKAYFGFTDAELEVLDKFVRNNTTERFGPVRAV
RADKDFGFVVEVAFEGINRSTRHKSGVAMRFPRIARLRPDKPTYEADRLRTLIAMIDAKPG
>Mature_541_residues
MKAFADLLDRLVLTPSRNGKLKLLTDYFRDTPDPDRGYGLAAIAGTLEVRNVKPAMLRELVLERMDEVLFRYSYDYVGDL
AETISLVWDNERDIDRSALSQPRLGTVVTDMNALGRTEVRGFVRDLLDRLDSSGRFAFIKLATGAMRIGVSARLAKQALA
DLGDKDVTEIETLWHGLQPPYETLFEWLAAGGEKPVLATPAIFHSVMLANAVEEGDLNGLDPASYAAEWKWDGIRVQLSR
SGDRRKIYSRSGDDISGAFPDILDAISFSGVVDGELLVGGTARSNSPTRTFSDLQQRLNRKTVTAKMLSDYPAFIRTYDL
LFDDDEDVRGRGYTDRRDRLAEIIDRAPHDRFDLSPLVPFSSWDELDRLRAAPPDPVIEGVMIKRRDSIYQAGRMKGPWF
KWKRNPYNVDAVLMYAQRGHGKRSSYYSDFTFGVWADDEDGEQLVPVGKAYFGFTDAELEVLDKFVRNNTTERFGPVRAV
RADKDFGFVVEVAFEGINRSTRHKSGVAMRFPRIARLRPDKPTYEADRLRTLIAMIDAKPG

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=413, Percent_Identity=26.1501210653753, Blast_Score=78, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71985269, Length=498, Percent_Identity=24.2971887550201, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320038, Length=365, Percent_Identity=25.4794520547945, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24762424, Length=335, Percent_Identity=23.2835820895522, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 61036; Mature: 61036

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFADLLDRLVLTPSRNGKLKLLTDYFRDTPDPDRGYGLAAIAGTLEVRNVKPAMLREL
CCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCCCCCCCCHHHHHCCCEECCCCHHHHHHH
VLERMDEVLFRYSYDYVGDLAETISLVWDNERDIDRSALSQPRLGTVVTDMNALGRTEVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHH
GFVRDLLDRLDSSGRFAFIKLATGAMRIGVSARLAKQALADLGDKDVTEIETLWHGLQPP
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
YETLFEWLAAGGEKPVLATPAIFHSVMLANAVEEGDLNGLDPASYAAEWKWDGIRVQLSR
HHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCEEEEEEC
SGDRRKIYSRSGDDISGAFPDILDAISFSGVVDGELLVGGTARSNSPTRTFSDLQQRLNR
CCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
KTVTAKMLSDYPAFIRTYDLLFDDDEDVRGRGYTDRRDRLAEIIDRAPHDRFDLSPLVPF
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
SSWDELDRLRAAPPDPVIEGVMIKRRDSIYQAGRMKGPWFKWKRNPYNVDAVLMYAQRGH
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHEEEEEHHCC
GKRSSYYSDFTFGVWADDEDGEQLVPVGKAYFGFTDAELEVLDKFVRNNTTERFGPVRAV
CCCCCCCCCCEEEEECCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCHHHCCEEEE
RADKDFGFVVEVAFEGINRSTRHKSGVAMRFPRIARLRPDKPTYEADRLRTLIAMIDAKP
ECCCCCCEEEEEEHHCCCCCHHCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHCCCC
G
C
>Mature Secondary Structure
MKAFADLLDRLVLTPSRNGKLKLLTDYFRDTPDPDRGYGLAAIAGTLEVRNVKPAMLREL
CCHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCCCCCCCCHHHHHCCCEECCCCHHHHHHH
VLERMDEVLFRYSYDYVGDLAETISLVWDNERDIDRSALSQPRLGTVVTDMNALGRTEVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHH
GFVRDLLDRLDSSGRFAFIKLATGAMRIGVSARLAKQALADLGDKDVTEIETLWHGLQPP
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
YETLFEWLAAGGEKPVLATPAIFHSVMLANAVEEGDLNGLDPASYAAEWKWDGIRVQLSR
HHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCEEEEEEC
SGDRRKIYSRSGDDISGAFPDILDAISFSGVVDGELLVGGTARSNSPTRTFSDLQQRLNR
CCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
KTVTAKMLSDYPAFIRTYDLLFDDDEDVRGRGYTDRRDRLAEIIDRAPHDRFDLSPLVPF
HHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
SSWDELDRLRAAPPDPVIEGVMIKRRDSIYQAGRMKGPWFKWKRNPYNVDAVLMYAQRGH
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHEEEEEHHCC
GKRSSYYSDFTFGVWADDEDGEQLVPVGKAYFGFTDAELEVLDKFVRNNTTERFGPVRAV
CCCCCCCCCCEEEEECCCCCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCHHHCCEEEE
RADKDFGFVVEVAFEGINRSTRHKSGVAMRFPRIARLRPDKPTYEADRLRTLIAMIDAKP
ECCCCCCEEEEEEHHCCCCCHHCCCCCEEECCHHHCCCCCCCCCHHHHHHHHHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA