Click here to switch to the map view.

The map label for this gene is smeIM [H]

Identifier: 209548255

GI number: 209548255

Start: 671098

End: 672240

Strand: Direct

Name: smeIM [H]

Synonym: Rleg2_0649

Alternate gene names: 209548255

Gene position: 671098-672240 (Clockwise)

Preceding gene: 209548254

Following gene: 209548259

Centisome position: 14.79

GC content: 61.33

Gene sequence:

>1143_bases
ATGGCATCAGTTTTTCCGCTTGCCGACCTCAAGGCTTCCATCAGGTCGGATTCCCGGCCGGGGTCTTGGGTCGACACGAT
CATCAAGGGTGATTGCGTCAGTGCCCTTGAGGCTTTGCCCACCCACTCCGTCGATGTCATCTTTGCCGATCCGCCGTATA
ACCTCCAGCTCGGCGGAACGCTGCATCGTCCTGACCAGTCGCTGGTCGATGCGGTCGATGACGAATGGGATCAGTTCGCC
TCCTTCGAGGCCTATGACGCCTTCACCCGCGCCTGGCTGCTCGCCTGCCGGCGGGTGTTGAAGCCGACCGGTTCGATCTG
GGTGATCGGTTCCTATCACAATATCTTCCGCGTCGGCGCCACGCTGCAGGATCTGAACTTCTGGATCCTCAACGACATCA
TTTGGCGCAAGACCAATCCGATGCCGAATTTCAAGGGCCGCCGTTTTCAGAACGCCCATGAGACGATGATCTGGGCGAGC
CCCAATGCCAAGGCCAAGGGCTATACCTTCAATTACGATGCGATGAAGGCCGCCAATGACGACGTGCAGATGCGCTCCGA
CTGGCTGTTCCCGATCTGCAACGGCAATGAGCGGCTGAAGGGCGAGGACGGCAAGAAGGTGCATCCGACGCAGAAGCCGG
AAGCCTTGCTTGCCCGCGTTATCATGGCCTCGACCAAACCAGGCGATATCGTGCTTGATCCCTTCTTCGGTTCGGGAACG
ACGGGCGCCGTCGCCAAGCGTCTCGGCCGCCACTTCGTCGGCATCGAGCGCGAGCAGGATTATATCGATGCGGCTTCGGC
CCGCATCGCCGCCGTCGAGCCGCTGGGGAAGGCGGAACTGACCGTCATGACCGGCAAGAAGGCCGAGGTTCGCGTCGCCT
TCAACGTGCTTGTCGAAAGCGGCCTGATCAAGCCCGGCCAGGTGCTGACCGATGCCAAACGCCGTTACAGCGCCATCGTG
CGCGCCGACGGCACGGTGGCTTCAGGCGGCGACGCCGGCTCCATTCATCGTCTCGGCGCCAAGGTGCAGGGTCTCGATGC
ATGCAACGGATGGACCTTCTGGCATTTCGAAGACGGGCAGTCCCTGCGCCCGATCGACGATCTCAGGTCCGTCATCCGCA
GTGATCTGGCAAAGGTGGAGTGA

Upstream 100 bases:

>100_bases
GCAATAAATTCCCGAATTCAACCACCGCTTCAGGCTTTGGTAACTAAGTTGGGTAACCATAACGGAGAGTTAAGCGTCAA
GCGTATGAGTGAGTATCAGA

Downstream 100 bases:

>100_bases
GCACCACCCAAATCAGAGAACTGCAGCATGATGTCGTCCGAAAACCGCTTACACTTTTCGGCATCATGCTCCGGCGGATC
GAAATACCTTCTTCCGGTTT

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MASVFPLADLKASIRSDSRPGSWVDTIIKGDCVSALEALPTHSVDVIFADPPYNLQLGGTLHRPDQSLVDAVDDEWDQFA
SFEAYDAFTRAWLLACRRVLKPTGSIWVIGSYHNIFRVGATLQDLNFWILNDIIWRKTNPMPNFKGRRFQNAHETMIWAS
PNAKAKGYTFNYDAMKAANDDVQMRSDWLFPICNGNERLKGEDGKKVHPTQKPEALLARVIMASTKPGDIVLDPFFGSGT
TGAVAKRLGRHFVGIEREQDYIDAASARIAAVEPLGKAELTVMTGKKAEVRVAFNVLVESGLIKPGQVLTDAKRRYSAIV
RADGTVASGGDAGSIHRLGAKVQGLDACNGWTFWHFEDGQSLRPIDDLRSVIRSDLAKVE

Sequences:

>Translated_380_residues
MASVFPLADLKASIRSDSRPGSWVDTIIKGDCVSALEALPTHSVDVIFADPPYNLQLGGTLHRPDQSLVDAVDDEWDQFA
SFEAYDAFTRAWLLACRRVLKPTGSIWVIGSYHNIFRVGATLQDLNFWILNDIIWRKTNPMPNFKGRRFQNAHETMIWAS
PNAKAKGYTFNYDAMKAANDDVQMRSDWLFPICNGNERLKGEDGKKVHPTQKPEALLARVIMASTKPGDIVLDPFFGSGT
TGAVAKRLGRHFVGIEREQDYIDAASARIAAVEPLGKAELTVMTGKKAEVRVAFNVLVESGLIKPGQVLTDAKRRYSAIV
RADGTVASGGDAGSIHRLGAKVQGLDACNGWTFWHFEDGQSLRPIDDLRSVIRSDLAKVE
>Mature_379_residues
ASVFPLADLKASIRSDSRPGSWVDTIIKGDCVSALEALPTHSVDVIFADPPYNLQLGGTLHRPDQSLVDAVDDEWDQFAS
FEAYDAFTRAWLLACRRVLKPTGSIWVIGSYHNIFRVGATLQDLNFWILNDIIWRKTNPMPNFKGRRFQNAHETMIWASP
NAKAKGYTFNYDAMKAANDDVQMRSDWLFPICNGNERLKGEDGKKVHPTQKPEALLARVIMASTKPGDIVLDPFFGSGTT
GAVAKRLGRHFVGIEREQDYIDAASARIAAVEPLGKAELTVMTGKKAEVRVAFNVLVESGLIKPGQVLTDAKRRYSAIVR
ADGTVASGGDAGSIHRLGAKVQGLDACNGWTFWHFEDGQSLRPIDDLRSVIRSDLAKVE

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=290, Percent_Identity=33.448275862069, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41917; Mature: 41786

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVFPLADLKASIRSDSRPGSWVDTIIKGDCVSALEALPTHSVDVIFADPPYNLQLGGT
CCCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEECCE
LHRPDQSLVDAVDDEWDQFASFEAYDAFTRAWLLACRRVLKPTGSIWVIGSYHNIFRVGA
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
TLQDLNFWILNDIIWRKTNPMPNFKGRRFQNAHETMIWASPNAKAKGYTFNYDAMKAAND
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHCCCC
DVQMRSDWLFPICNGNERLKGEDGKKVHPTQKPEALLARVIMASTKPGDIVLDPFFGSGT
CCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
TGAVAKRLGRHFVGIEREQDYIDAASARIAAVEPLGKAELTVMTGKKAEVRVAFNVLVES
HHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCCEEEEEECCCCEEEEHEEHHHHH
GLIKPGQVLTDAKRRYSAIVRADGTVASGGDAGSIHRLGAKVQGLDACNGWTFWHFEDGQ
CCCCCCHHHHHHHHHHHHHEEECCCEECCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCC
SLRPIDDLRSVIRSDLAKVE
CCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASVFPLADLKASIRSDSRPGSWVDTIIKGDCVSALEALPTHSVDVIFADPPYNLQLGGT
CCCCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCCCEEECCE
LHRPDQSLVDAVDDEWDQFASFEAYDAFTRAWLLACRRVLKPTGSIWVIGSYHNIFRVGA
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCC
TLQDLNFWILNDIIWRKTNPMPNFKGRRFQNAHETMIWASPNAKAKGYTFNYDAMKAAND
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEECCHHHHCCCC
DVQMRSDWLFPICNGNERLKGEDGKKVHPTQKPEALLARVIMASTKPGDIVLDPFFGSGT
CCEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCC
TGAVAKRLGRHFVGIEREQDYIDAASARIAAVEPLGKAELTVMTGKKAEVRVAFNVLVES
HHHHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCCEEEEEECCCCEEEEHEEHHHHH
GLIKPGQVLTDAKRRYSAIVRADGTVASGGDAGSIHRLGAKVQGLDACNGWTFWHFEDGQ
CCCCCCHHHHHHHHHHHHHEEECCCEECCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCC
SLRPIDDLRSVIRSDLAKVE
CCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]