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The map label for this gene is gcvA [H]

Identifier: 209548185

GI number: 209548185

Start: 603778

End: 604671

Strand: Direct

Name: gcvA [H]

Synonym: Rleg2_0579

Alternate gene names: 209548185

Gene position: 603778-604671 (Clockwise)

Preceding gene: 209548182

Following gene: 209548186

Centisome position: 13.31

GC content: 61.19

Gene sequence:

>894_bases
ATGCAACTGCCGCCGCTGAACGCCTTAAGAGCCTTCGAAGCGACCGCTCGGCTGATGAGCCTGTCAAAGGCCGGAGACGA
ACTGCATGTGACCCACGCGGCCATCAGTCATCAGCTCAGACATCTGGAGGCGTGGCTCGGCCGCAAGCTGCTGCAAAAAT
CCGGGCGCGGCGTGGCGCTGACCCCGGCGGGCAGCGAATATTATCTGGCTGTCTCGGGCTCACTGGCAGCCATCGCCCAC
GCCACCGGCAACATGCGCAGAGACCACAATATGCGGTCGATCACCGTTGGATGCATTCCTTCGATCGCGTCGCGCTGGCT
GGTTCCGGCGCTGCCGTCTTTCCGAGAGAGCGATCCCGATCTCGACGTCAGGATCGTCTATGCAAAGGCGGAAGAGCGCT
TCGACGACGAAAGCCTGGACGTCCTGATTACCATGGGAGAAGACCTGTCGGGGGGACGGGAGAGCCGCCTGCTTTTCTCC
CGCCGCAACCAGCCGGTGGCGAGCATTCATTATCTGGCCAAGCGGAATTGGACTGTCGGCAGCGTTCCCTTTGCAGCGGC
CGACCTGCTGCACGACGAGTCCGTCGACGGCTGGAAGGAATGGTTCCGAAAGACTGATATAAAGGCGCCCGAGCCGGTTC
GCGGGCCGATTTTTCAGGACTTCAACATTCTTGCCACGGCGGTGATCGCTGGGCATGGGGTGGCATTATGCCCGGTCGAA
GTGTTTCGGCGCGAGATCGATCGCGGCGACCTCGTCGTGCTGTCGGACATCTCGACGGCGGAGACGCAGGGATATTACCT
GATCACCAGCGCCTGGGCGAAAAAGCCGGTGCGTCGTTTTACGGAGTGGTTTCTGAGGGAATGCCGATCCGCCGGTTCGC
CGATCATTGAATAA

Upstream 100 bases:

>100_bases
ATGCGAGAATTCCGTTGAGCCGATATCAGCAAGTTTTTACTAGGTTTTGACATTTCTGGTGGCGAATAAACCCTGCCGCT
CATGTAAGGAATACGAACAG

Downstream 100 bases:

>100_bases
GCTCTGCCAAGGCGGGCGCTCAAAAGGGCGGATGGCAAACGCATCGGCTCAGACAGCTGCAGCGACAGCAGCGCAGAGTC
GGGGGCGGTGACAAGAGAGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQLRHLEAWLGRKLLQKSGRGVALTPAGSEYYLAVSGSLAAIAH
ATGNMRRDHNMRSITVGCIPSIASRWLVPALPSFRESDPDLDVRIVYAKAEERFDDESLDVLITMGEDLSGGRESRLLFS
RRNQPVASIHYLAKRNWTVGSVPFAAADLLHDESVDGWKEWFRKTDIKAPEPVRGPIFQDFNILATAVIAGHGVALCPVE
VFRREIDRGDLVVLSDISTAETQGYYLITSAWAKKPVRRFTEWFLRECRSAGSPIIE

Sequences:

>Translated_297_residues
MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQLRHLEAWLGRKLLQKSGRGVALTPAGSEYYLAVSGSLAAIAH
ATGNMRRDHNMRSITVGCIPSIASRWLVPALPSFRESDPDLDVRIVYAKAEERFDDESLDVLITMGEDLSGGRESRLLFS
RRNQPVASIHYLAKRNWTVGSVPFAAADLLHDESVDGWKEWFRKTDIKAPEPVRGPIFQDFNILATAVIAGHGVALCPVE
VFRREIDRGDLVVLSDISTAETQGYYLITSAWAKKPVRRFTEWFLRECRSAGSPIIE
>Mature_297_residues
MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQLRHLEAWLGRKLLQKSGRGVALTPAGSEYYLAVSGSLAAIAH
ATGNMRRDHNMRSITVGCIPSIASRWLVPALPSFRESDPDLDVRIVYAKAEERFDDESLDVLITMGEDLSGGRESRLLFS
RRNQPVASIHYLAKRNWTVGSVPFAAADLLHDESVDGWKEWFRKTDIKAPEPVRGPIFQDFNILATAVIAGHGVALCPVE
VFRREIDRGDLVVLSDISTAETQGYYLITSAWAKKPVRRFTEWFLRECRSAGSPIIE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=33.2167832167832, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=29.616724738676, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=25.1677852348993, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788887, Length=263, Percent_Identity=26.615969581749, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=24.4147157190635, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787589, Length=277, Percent_Identity=25.2707581227437, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=174, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786401, Length=240, Percent_Identity=23.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33030; Mature: 33030

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQLRHLEAWLGRKLLQKSGRGVAL
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TPAGSEYYLAVSGSLAAIAHATGNMRRDHNMRSITVGCIPSIASRWLVPALPSFRESDPD
ECCCCEEEEEECCCHHEEHHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCC
LDVRIVYAKAEERFDDESLDVLITMGEDLSGGRESRLLFSRRNQPVASIHYLAKRNWTVG
CEEEEEEECCHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
SVPFAAADLLHDESVDGWKEWFRKTDIKAPEPVRGPIFQDFNILATAVIAGHGVALCPVE
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECHHH
VFRREIDRGDLVVLSDISTAETQGYYLITSAWAKKPVRRFTEWFLRECRSAGSPIIE
HHHHHCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQLPPLNALRAFEATARLMSLSKAGDELHVTHAAISHQLRHLEAWLGRKLLQKSGRGVAL
CCCCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TPAGSEYYLAVSGSLAAIAHATGNMRRDHNMRSITVGCIPSIASRWLVPALPSFRESDPD
ECCCCEEEEEECCCHHEEHHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCCCCC
LDVRIVYAKAEERFDDESLDVLITMGEDLSGGRESRLLFSRRNQPVASIHYLAKRNWTVG
CEEEEEEECCHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCC
SVPFAAADLLHDESVDGWKEWFRKTDIKAPEPVRGPIFQDFNILATAVIAGHGVALCPVE
CCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECHHH
VFRREIDRGDLVVLSDISTAETQGYYLITSAWAKKPVRRFTEWFLRECRSAGSPIIE
HHHHHCCCCCEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]