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The map label for this gene is ygcA [C]

Identifier: 209548166

GI number: 209548166

Start: 581333

End: 582583

Strand: Reverse

Name: ygcA [C]

Synonym: Rleg2_0560

Alternate gene names: 209548166

Gene position: 582583-581333 (Counterclockwise)

Preceding gene: 209548167

Following gene: 209548164

Centisome position: 12.84

GC content: 66.27

Gene sequence:

>1251_bases
GTGAGCACCGAAACCGTCACCATCGAGAAGCTCGGCGCCCAGGGCGACGGCATTGCCGGCAGCGCCGGCGGGCCAATCTA
TGTGCCATTCGCCCTGCCGGGAGAAACCGTGGCGATCGCCCGCGTCAAAAGCCAGGGCACGATCATGTCGATCGCAACGC
CCTCGCCCGACCGGCAGGAGCCGCCCTGCCGGCATTTCGGCCCGGATGGCGTCAACGGCACCTGCGGCGGCTGCACGCTG
CAGCATATGACCGATAGCCCCTACCGGGCCTTCAAGCGCCAGCTGGTCATCGATGCGCTCAGATCGAAGGGGCTGACGCC
CGATGTCGGCGAGATCGTTGCGGCCCGGCCGGGCGAACGCCGGCGCGTGGTGTTTGCGGCGCGCAAGACCGAAAAGGACA
TGCTGATCGGTTTCAACCAGGCCGAAAGCCATCATATCGTCGCCATCGAGGAATGTCCGATCTCGTCGGCGGGCATTATC
GCCCGGCTGCCGGCGATCCGGGCGATTGCCGCAGCACTGGCGACCAATGCCGAGCCCTTCCGCGTCTCGGTGCTCGAAAC
ACTGTCCGGCCTCGATATCGCGGTCGACGAGGTGAAAAAACTCTCCGATGCGCAGCGGCGCAAAGCGGTTGAGACCGTGC
TCGGCCTGCGCGGCATTGCCCGTGTTTCGCTGAACGGCGAAATCCTCATCGAGCCGTCGAAGCCGCTCGTCGATTTCGGC
GGGATCCAGGTTTCCCCGCCGCCGGGCGGCTTCACCCAGGCGACCAAGCCGGCTGAGGAAGCGATGGCCGAGCTGGTGCT
TGCCCATGCCGGCAAGGCCAAGCGGATCGCCGATCTTTTTGCCGGCGCCGGCACGTTTTCGCTGCGGCTGGCGCGGATCG
GCCGCGTGCATGCCGTCGAAGCCGAAGCAAAAGCGCTCGCTGCCCTCGATCATGCCGCCCGCAACGCTCCTGGATTGAAG
CCGGTGACCATCGAGAGGCGCGATCTCTTCCGCCGCCCGTTGATGACGCAGGAGTTCAAGCCCTATGACGCCGTCATCTT
CGATCCGCCACGCGCCGGCGCCGAGTTCCAGTGCAAGGAGCTTGCCCGCTCGAGCGTGAAAAAGATCGCCGCCGTCAGCT
GCAACCCGCTGACGCTGGCGCGCGACCTCGCGATCCTCGTCGAGGGCGGCTACCGGATCACCGGCGTGACGCCGATCGAC
CAGTTCCTCTGGACCTCGCATGTCGAGGTGGTGGCGACGCTGGAGAAATAG

Upstream 100 bases:

>100_bases
GGTTCATCGAGGATATGGGCTGGAAAAGCCTCGGCCTCATCCCCTCTCCGATTGCCGGCGGCGACGGCAATCAGGAATAT
CTTCTGGCAGGATCGAAACC

Downstream 100 bases:

>100_bases
GCGGGCCGCCGTCGGCTGCCCCCGCTATTGGTCGCCGCAGTCCCGTTCCTCACCGGCACTCAGCCGCATTGCCCGGGTTC
CGAGTTTGGCACCTGTCACC

Product: (uracil-5)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSTETVTIEKLGAQGDGIAGSAGGPIYVPFALPGETVAIARVKSQGTIMSIATPSPDRQEPPCRHFGPDGVNGTCGGCTL
QHMTDSPYRAFKRQLVIDALRSKGLTPDVGEIVAARPGERRRVVFAARKTEKDMLIGFNQAESHHIVAIEECPISSAGII
ARLPAIRAIAAALATNAEPFRVSVLETLSGLDIAVDEVKKLSDAQRRKAVETVLGLRGIARVSLNGEILIEPSKPLVDFG
GIQVSPPPGGFTQATKPAEEAMAELVLAHAGKAKRIADLFAGAGTFSLRLARIGRVHAVEAEAKALAALDHAARNAPGLK
PVTIERRDLFRRPLMTQEFKPYDAVIFDPPRAGAEFQCKELARSSVKKIAAVSCNPLTLARDLAILVEGGYRITGVTPID
QFLWTSHVEVVATLEK

Sequences:

>Translated_416_residues
MSTETVTIEKLGAQGDGIAGSAGGPIYVPFALPGETVAIARVKSQGTIMSIATPSPDRQEPPCRHFGPDGVNGTCGGCTL
QHMTDSPYRAFKRQLVIDALRSKGLTPDVGEIVAARPGERRRVVFAARKTEKDMLIGFNQAESHHIVAIEECPISSAGII
ARLPAIRAIAAALATNAEPFRVSVLETLSGLDIAVDEVKKLSDAQRRKAVETVLGLRGIARVSLNGEILIEPSKPLVDFG
GIQVSPPPGGFTQATKPAEEAMAELVLAHAGKAKRIADLFAGAGTFSLRLARIGRVHAVEAEAKALAALDHAARNAPGLK
PVTIERRDLFRRPLMTQEFKPYDAVIFDPPRAGAEFQCKELARSSVKKIAAVSCNPLTLARDLAILVEGGYRITGVTPID
QFLWTSHVEVVATLEK
>Mature_415_residues
STETVTIEKLGAQGDGIAGSAGGPIYVPFALPGETVAIARVKSQGTIMSIATPSPDRQEPPCRHFGPDGVNGTCGGCTLQ
HMTDSPYRAFKRQLVIDALRSKGLTPDVGEIVAARPGERRRVVFAARKTEKDMLIGFNQAESHHIVAIEECPISSAGIIA
RLPAIRAIAAALATNAEPFRVSVLETLSGLDIAVDEVKKLSDAQRRKAVETVLGLRGIARVSLNGEILIEPSKPLVDFGG
IQVSPPPGGFTQATKPAEEAMAELVLAHAGKAKRIADLFAGAGTFSLRLARIGRVHAVEAEAKALAALDHAARNAPGLKP
VTIERRDLFRRPLMTQEFKPYDAVIFDPPRAGAEFQCKELARSSVKKIAAVSCNPLTLARDLAILVEGGYRITGVTPIDQ
FLWTSHVEVVATLEK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=433, Percent_Identity=29.5612009237875, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 44320; Mature: 44188

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTETVTIEKLGAQGDGIAGSAGGPIYVPFALPGETVAIARVKSQGTIMSIATPSPDRQE
CCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCC
PPCRHFGPDGVNGTCGGCTLQHMTDSPYRAFKRQLVIDALRSKGLTPDVGEIVAARPGER
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
RRVVFAARKTEKDMLIGFNQAESHHIVAIEECPISSAGIIARLPAIRAIAAALATNAEPF
CEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
RVSVLETLSGLDIAVDEVKKLSDAQRRKAVETVLGLRGIARVSLNGEILIEPSKPLVDFG
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCEECC
GIQVSPPPGGFTQATKPAEEAMAELVLAHAGKAKRIADLFAGAGTFSLRLARIGRVHAVE
CEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEEEEHHCCEEEEH
AEAKALAALDHAARNAPGLKPVTIERRDLFRRPLMTQEFKPYDAVIFDPPRAGAEFQCKE
HHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
LARSSVKKIAAVSCNPLTLARDLAILVEGGYRITGVTPIDQFLWTSHVEVVATLEK
HHHHHHHHHHHCCCCCHHHHHHEEEEEECCEEEEECCHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure 
STETVTIEKLGAQGDGIAGSAGGPIYVPFALPGETVAIARVKSQGTIMSIATPSPDRQE
CCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCC
PPCRHFGPDGVNGTCGGCTLQHMTDSPYRAFKRQLVIDALRSKGLTPDVGEIVAARPGER
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
RRVVFAARKTEKDMLIGFNQAESHHIVAIEECPISSAGIIARLPAIRAIAAALATNAEPF
CEEEEEECCCCCCEEEEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCE
RVSVLETLSGLDIAVDEVKKLSDAQRRKAVETVLGLRGIARVSLNGEILIEPSKPLVDFG
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCCCCEECC
GIQVSPPPGGFTQATKPAEEAMAELVLAHAGKAKRIADLFAGAGTFSLRLARIGRVHAVE
CEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEEEEEHHCCEEEEH
AEAKALAALDHAARNAPGLKPVTIERRDLFRRPLMTQEFKPYDAVIFDPPRAGAEFQCKE
HHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
LARSSVKKIAAVSCNPLTLARDLAILVEGGYRITGVTPIDQFLWTSHVEVVATLEK
HHHHHHHHHHHCCCCCHHHHHHEEEEEECCEEEEECCHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]