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The map label for this gene is glyS [H]

Identifier: 209548144

GI number: 209548144

Start: 554083

End: 556266

Strand: Reverse

Name: glyS [H]

Synonym: Rleg2_0538

Alternate gene names: 209548144

Gene position: 556266-554083 (Counterclockwise)

Preceding gene: 209548150

Following gene: 209548132

Centisome position: 12.26

GC content: 64.38

Gene sequence:

>2184_bases
ATGCCAAACCTTCTTCTCGAACTCCGCTCCGAAGAGATTCCGGCCCGCATGCAGCGCAAGGCTGCCGGCGACCTGAAGAA
GCTCGTCACCGATGCGCTTGTCGAAGCGGGTCTTTCCTATGAGGGCGCCCGCGAATACTGGACGCCGCGGCGGCTGGCGC
TCGACATTCACGGGTTGACGGCGCGCTCGGCCGACGTGCGCGAGGAGCGCAAGGGGCCGCGCACCGACGCCAATGAGAAG
GCAATCGAAGGCTTTTTGCGCGGCGCCGGCCTTTCGTCGGTCTCCGAGGCGCAGGTGGTCAGCGATCCGAAGAAGGGCGA
TTTCTACGTTGCGGTCATTTCCAAGCCCGGCCGCGCTGCCGAAGAGATCGTCGCCGAGGTGATGCCCGGCATCATCCGCG
ATTTTCCCTGGCCGAAGTCGATGCGCTGGGGCAAGGCCTCCTCCAGGCCAGGGGCGATGCGCTGGGTGCGGCCGCTGCAG
TCGATCGTCTGCACTTTCGGCCCGGAGCATGAAGAGACCGTCGTCATCCCCTTCGAGATCGACGGCATCACCGCCTCCAA
CACCACCTATGGCCATCGTTTCCACGCGCCCGAGGCGATCACCGTCCGGCGCTTCGACGATTATGCGGCAAGCCTGGAAA
AGGCCAAGGTCATCCTCGACGCCGAGCGGCGCAAGGACATCATCCTGCACGACGCCCGCGACATCGCCTTCGCAAACGGG
CTGGAGCTGGTGGAGGACGAGGGCCTGCTGGAGGAAGTTTCCGGCCTCGTCGAATGGCCGCAGGTGCTGATGGGTTCTTT
TGAGGAGGATTATCTGTCGATCCCCTCCGAGATCATCCGACTGACCATCAAGACCAACCAGAAGTGCTTCGTCACCCGCA
AGCAGGGTGAGGAAACGCTGTCGAACAAGTTCATTCTGGTTTCCAATATCCAGGCGCATGACGGCGGCAAGGAAATCGTC
CACGGCAACGGCAAGGTGGTACGAGCCCGGCTTTCGGATGCGCTGCATTTCTGGAAGCGCGACCAGGGCGACCTGCCGGA
TCTGGAGACGCTGACGGCGTCGGCGGCAAAATTCGGTCTCGACCTGAAGAAGCCGCTCGACCAGCGCATGGCCAAGCTCG
ATGCGCTCGACGTGACCTTCCATGCCAAGCTCGGCACGCAGGGCGCACGCGTCGCCCGCATCCGGGCGCTGGCGGAAGAA
CTGGCAAAGATCACCGGCGCAGATGCCGCCCTCACCGATCGCGCCGCCGTGCTTGCCAAGGCCGATCTTCGCACCGAAGC
GGTCGGCGAATTCCCCGAGCTGCAGGGCCTGATGGGCCGCAAATATGCGGGGCTGCAGGGCGAGAATGGCTCGGTCGCCG
CAGCGATCGAGGACCATTACAAGCCGCAAGGCCCGTCCGACCGCGTTCCCGCCGATAAGGTCGCGATCACCGTCGCGCTC
GCCGACAAGCTGGACACGCTGATCGGCTTCTGGGCGATCGGCGAAAAGCCGACGGGTTCGAAGGACCCCTTTGCGCTGCG
GCGTGCTGCTCTCGGCGTCGTCAGGATCCTGCTGGAGCGCAATGTCAGGCTGCCGCTGCTGACGATCATGAGCCAAACGT
CGCTGCTCGCCAAGACAGGCGGCGACACCGTGTTTGCATTCGCAGGCGATCCGGCCGAGACTTTCTTCGACCTCCTCTCC
TTCTTCCACGACCGCCTCAAGGTCTATCTGCGCGACCAGGGCGCCCGCCACGATCTGATCGACGCGGTGCTGACTTCAGA
TGCCGACGACCTGTTGATGGTCGCGCGCCGCGTCGAAGCGCTGACGGCCTTCATCACCTCGGAAGACGGCAAGAACCTGC
TTGCCGGCACCAAGCGCGCCACGCAGCTGCTCGCCGCCGAGGAGAAAAAGGGCACCGTGATCGCCGATGCCGTTTCACCG
GCGCTGCTGAAACTCGACGCCGAGAAGGAGCTGTTCGCGGCGATTTCAGGCGCATCGAAAGAAGCGGCCGATGCCGTCGC
CAGCGAAGATTTCCGCTCGGCAATGGCCGCCCTTTCCAAGCTGCGCGGCCCGGTCGACCGCTTCTTCGAAGACGTGCTCG
TCAACGACGAGGACGCCGCCATCCGCGCCAACCGCCTCGCTCTGCTGCGGCTGATCCGCGAAGCGACGGGGACGGTTGCG
GATTTCTCGAAGATTTCGGGGTGA

Upstream 100 bases:

>100_bases
GCCCTCTTCCGTTTTTGCGGTTGTGCAGATGCGGCATTCTTTGATAGCAGAAGCAACGTTTTTGGATCGCCCTGCCCGCG
GGCGCAAGGAAAAGAGTGAC

Downstream 100 bases:

>100_bases
GAGCCGGGTTTTGGCCGGGTGCATGAGGTGACGGAGTGCTTACGGCCGTGGGTCCTCGGGTCAAGCCCGAGGACGACGGC
GGGTGGGGGAATGCATGCTG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MPNLLLELRSEEIPARMQRKAAGDLKKLVTDALVEAGLSYEGAREYWTPRRLALDIHGLTARSADVREERKGPRTDANEK
AIEGFLRGAGLSSVSEAQVVSDPKKGDFYVAVISKPGRAAEEIVAEVMPGIIRDFPWPKSMRWGKASSRPGAMRWVRPLQ
SIVCTFGPEHEETVVIPFEIDGITASNTTYGHRFHAPEAITVRRFDDYAASLEKAKVILDAERRKDIILHDARDIAFANG
LELVEDEGLLEEVSGLVEWPQVLMGSFEEDYLSIPSEIIRLTIKTNQKCFVTRKQGEETLSNKFILVSNIQAHDGGKEIV
HGNGKVVRARLSDALHFWKRDQGDLPDLETLTASAAKFGLDLKKPLDQRMAKLDALDVTFHAKLGTQGARVARIRALAEE
LAKITGADAALTDRAAVLAKADLRTEAVGEFPELQGLMGRKYAGLQGENGSVAAAIEDHYKPQGPSDRVPADKVAITVAL
ADKLDTLIGFWAIGEKPTGSKDPFALRRAALGVVRILLERNVRLPLLTIMSQTSLLAKTGGDTVFAFAGDPAETFFDLLS
FFHDRLKVYLRDQGARHDLIDAVLTSDADDLLMVARRVEALTAFITSEDGKNLLAGTKRATQLLAAEEKKGTVIADAVSP
ALLKLDAEKELFAAISGASKEAADAVASEDFRSAMAALSKLRGPVDRFFEDVLVNDEDAAIRANRLALLRLIREATGTVA
DFSKISG

Sequences:

>Translated_727_residues
MPNLLLELRSEEIPARMQRKAAGDLKKLVTDALVEAGLSYEGAREYWTPRRLALDIHGLTARSADVREERKGPRTDANEK
AIEGFLRGAGLSSVSEAQVVSDPKKGDFYVAVISKPGRAAEEIVAEVMPGIIRDFPWPKSMRWGKASSRPGAMRWVRPLQ
SIVCTFGPEHEETVVIPFEIDGITASNTTYGHRFHAPEAITVRRFDDYAASLEKAKVILDAERRKDIILHDARDIAFANG
LELVEDEGLLEEVSGLVEWPQVLMGSFEEDYLSIPSEIIRLTIKTNQKCFVTRKQGEETLSNKFILVSNIQAHDGGKEIV
HGNGKVVRARLSDALHFWKRDQGDLPDLETLTASAAKFGLDLKKPLDQRMAKLDALDVTFHAKLGTQGARVARIRALAEE
LAKITGADAALTDRAAVLAKADLRTEAVGEFPELQGLMGRKYAGLQGENGSVAAAIEDHYKPQGPSDRVPADKVAITVAL
ADKLDTLIGFWAIGEKPTGSKDPFALRRAALGVVRILLERNVRLPLLTIMSQTSLLAKTGGDTVFAFAGDPAETFFDLLS
FFHDRLKVYLRDQGARHDLIDAVLTSDADDLLMVARRVEALTAFITSEDGKNLLAGTKRATQLLAAEEKKGTVIADAVSP
ALLKLDAEKELFAAISGASKEAADAVASEDFRSAMAALSKLRGPVDRFFEDVLVNDEDAAIRANRLALLRLIREATGTVA
DFSKISG
>Mature_726_residues
PNLLLELRSEEIPARMQRKAAGDLKKLVTDALVEAGLSYEGAREYWTPRRLALDIHGLTARSADVREERKGPRTDANEKA
IEGFLRGAGLSSVSEAQVVSDPKKGDFYVAVISKPGRAAEEIVAEVMPGIIRDFPWPKSMRWGKASSRPGAMRWVRPLQS
IVCTFGPEHEETVVIPFEIDGITASNTTYGHRFHAPEAITVRRFDDYAASLEKAKVILDAERRKDIILHDARDIAFANGL
ELVEDEGLLEEVSGLVEWPQVLMGSFEEDYLSIPSEIIRLTIKTNQKCFVTRKQGEETLSNKFILVSNIQAHDGGKEIVH
GNGKVVRARLSDALHFWKRDQGDLPDLETLTASAAKFGLDLKKPLDQRMAKLDALDVTFHAKLGTQGARVARIRALAEEL
AKITGADAALTDRAAVLAKADLRTEAVGEFPELQGLMGRKYAGLQGENGSVAAAIEDHYKPQGPSDRVPADKVAITVALA
DKLDTLIGFWAIGEKPTGSKDPFALRRAALGVVRILLERNVRLPLLTIMSQTSLLAKTGGDTVFAFAGDPAETFFDLLSF
FHDRLKVYLRDQGARHDLIDAVLTSDADDLLMVARRVEALTAFITSEDGKNLLAGTKRATQLLAAEEKKGTVIADAVSPA
LLKLDAEKELFAAISGASKEAADAVASEDFRSAMAALSKLRGPVDRFFEDVLVNDEDAAIRANRLALLRLIREATGTVAD
FSKISG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=729, Percent_Identity=34.7050754458162, Blast_Score=349, Evalue=5e-97,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 79360; Mature: 79229

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLLLELRSEEIPARMQRKAAGDLKKLVTDALVEAGLSYEGAREYWTPRRLALDIHGLT
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCC
ARSADVREERKGPRTDANEKAIEGFLRGAGLSSVSEAQVVSDPKKGDFYVAVISKPGRAA
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHH
EEIVAEVMPGIIRDFPWPKSMRWGKASSRPGAMRWVRPLQSIVCTFGPEHEETVVIPFEI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DGITASNTTYGHRFHAPEAITVRRFDDYAASLEKAKVILDAERRKDIILHDARDIAFANG
CCEEECCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHEEEHHCCCCEEEECCCHHHHHCC
LELVEDEGLLEEVSGLVEWPQVLMGSFEEDYLSIPSEIIRLTIKTNQKCFVTRKQGEETL
CHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHEEEEEECCCEEEEECCCCHHHH
SNKFILVSNIQAHDGGKEIVHGNGKVVRARLSDALHFWKRDQGDLPDLETLTASAAKFGL
HCCEEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
DLKKPLDQRMAKLDALDVTFHAKLGTQGARVARIRALAEELAKITGADAALTDRAAVLAK
CHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADLRTEAVGEFPELQGLMGRKYAGLQGENGSVAAAIEDHYKPQGPSDRVPADKVAITVAL
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCEEEEEEH
ADKLDTLIGFWAIGEKPTGSKDPFALRRAALGVVRILLERNVRLPLLTIMSQTSLLAKTG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GDTVFAFAGDPAETFFDLLSFFHDRLKVYLRDQGARHDLIDAVLTSDADDLLMVARRVEA
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
LTAFITSEDGKNLLAGTKRATQLLAAEEKKGTVIADAVSPALLKLDAEKELFAAISGASK
HHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCEEECCCCCHHEEECCHHHHHHHHCCCCH
EAADAVASEDFRSAMAALSKLRGPVDRFFEDVLVNDEDAAIRANRLALLRLIREATGTVA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
DFSKISG
HHHHCCC
>Mature Secondary Structure 
PNLLLELRSEEIPARMQRKAAGDLKKLVTDALVEAGLSYEGAREYWTPRRLALDIHGLT
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEEECCC
ARSADVREERKGPRTDANEKAIEGFLRGAGLSSVSEAQVVSDPKKGDFYVAVISKPGRAA
CCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEEEEECCCCCHH
EEIVAEVMPGIIRDFPWPKSMRWGKASSRPGAMRWVRPLQSIVCTFGPEHEETVVIPFEI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DGITASNTTYGHRFHAPEAITVRRFDDYAASLEKAKVILDAERRKDIILHDARDIAFANG
CCEEECCCCCCCEECCCCEEEEEEHHHHHHHHHHHHHEEEHHCCCCEEEECCCHHHHHCC
LELVEDEGLLEEVSGLVEWPQVLMGSFEEDYLSIPSEIIRLTIKTNQKCFVTRKQGEETL
CHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHEEEEEECCCEEEEECCCCHHHH
SNKFILVSNIQAHDGGKEIVHGNGKVVRARLSDALHFWKRDQGDLPDLETLTASAAKFGL
HCCEEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCC
DLKKPLDQRMAKLDALDVTFHAKLGTQGARVARIRALAEELAKITGADAALTDRAAVLAK
CHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADLRTEAVGEFPELQGLMGRKYAGLQGENGSVAAAIEDHYKPQGPSDRVPADKVAITVAL
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCEEEEEEH
ADKLDTLIGFWAIGEKPTGSKDPFALRRAALGVVRILLERNVRLPLLTIMSQTSLLAKTG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
GDTVFAFAGDPAETFFDLLSFFHDRLKVYLRDQGARHDLIDAVLTSDADDLLMVARRVEA
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
LTAFITSEDGKNLLAGTKRATQLLAAEEKKGTVIADAVSPALLKLDAEKELFAAISGASK
HHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCCEEECCCCCHHEEECCHHHHHHHHCCCCH
EAADAVASEDFRSAMAALSKLRGPVDRFFEDVLVNDEDAAIRANRLALLRLIREATGTVA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHH
DFSKISG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA