Click here to switch to the map view.

The map label for this gene is tag [H]

Identifier: 209548064

GI number: 209548064

Start: 468692

End: 469321

Strand: Direct

Name: tag [H]

Synonym: Rleg2_0457

Alternate gene names: 209548064

Gene position: 468692-469321 (Clockwise)

Preceding gene: 209548060

Following gene: 209548065

Centisome position: 10.33

GC content: 60.63

Gene sequence:

>630_bases
ATGAGCGAGACAGGCATCATCATCGGCGAGGATGGCAGGAGCCGCTGCCACTGGCACGCCAATCTGCCGGATTATCTCCG
CTATCATGACGAGGAATGGGGCCGCCCTGTCACCAATGATATCCGCCTCTTCGAGAAGATCTGCCTCGAAGGCTTCCAGT
CCGGCCTTTCCTGGCTGACCATCCTGCGCAAGCGCGAGAATTTCCGCGCCGCCTTTTCCGGCTTCGATTTCGAAAAGGTC
GCCCTGTTCGGCGACCAGGACGTCACCCGCTGCCTTGCCGATGCCGGCATCATCCGCCATCGCGGCAAGATCGTCTCCAC
CATCAACAATGCCAGGCGCGCCATCGCGCTGAGAGACGAATTCGGTTCGCTCGCCCATTATTTCTGGCGTTACGAGCCGG
GCCACAACGAGCGGCCTGGAGTGGTCGATCGCGAGCATATCGTTGCCAATCCGACGACGCCGACCTCGACGATCATTTCC
AAGGATCTGAAGAAACGCGGCTGGACCTTTGTCGGCCCGACCACAGTTTATGCTTTCATGCAGGCGATGGGCCTCGTCAA
CGATCACCTGGAGGGCTGCTTCTGCCGGCCCGAGGTCGAGGCGATGCGCGCCGTTTTGGTCCGACCATGA

Upstream 100 bases:

>100_bases
GTTCGGTTTGGAAAGACGGTTTGCAAATGGTAAAGCCGGAATCATGACCGGAAAGCCAAGCGGACCGGCAAAAACAGACG
GGCCCGGGAATGAAGCGGAC

Downstream 100 bases:

>100_bases
AAGAAGATGCATCCGTGAGAACATTGATCGCCGCCGCTGCCCTCCTTCTCCTGACGGTCCCCGCTCTGGCGCAGACGCCG
CAGCTCGATCCCGGCGAAAA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSETGIIIGEDGRSRCHWHANLPDYLRYHDEEWGRPVTNDIRLFEKICLEGFQSGLSWLTILRKRENFRAAFSGFDFEKV
ALFGDQDVTRCLADAGIIRHRGKIVSTINNARRAIALRDEFGSLAHYFWRYEPGHNERPGVVDREHIVANPTTPTSTIIS
KDLKKRGWTFVGPTTVYAFMQAMGLVNDHLEGCFCRPEVEAMRAVLVRP

Sequences:

>Translated_209_residues
MSETGIIIGEDGRSRCHWHANLPDYLRYHDEEWGRPVTNDIRLFEKICLEGFQSGLSWLTILRKRENFRAAFSGFDFEKV
ALFGDQDVTRCLADAGIIRHRGKIVSTINNARRAIALRDEFGSLAHYFWRYEPGHNERPGVVDREHIVANPTTPTSTIIS
KDLKKRGWTFVGPTTVYAFMQAMGLVNDHLEGCFCRPEVEAMRAVLVRP
>Mature_208_residues
SETGIIIGEDGRSRCHWHANLPDYLRYHDEEWGRPVTNDIRLFEKICLEGFQSGLSWLTILRKRENFRAAFSGFDFEKVA
LFGDQDVTRCLADAGIIRHRGKIVSTINNARRAIALRDEFGSLAHYFWRYEPGHNERPGVVDREHIVANPTTPTSTIISK
DLKKRGWTFVGPTTVYAFMQAMGLVNDHLEGCFCRPEVEAMRAVLVRP

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=184, Percent_Identity=50, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 23939; Mature: 23807

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETGIIIGEDGRSRCHWHANLPDYLRYHDEEWGRPVTNDIRLFEKICLEGFQSGLSWLT
CCCCCEEEECCCCCEEEEECCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ILRKRENFRAAFSGFDFEKVALFGDQDVTRCLADAGIIRHRGKIVSTINNARRAIALRDE
HHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FGSLAHYFWRYEPGHNERPGVVDREHIVANPTTPTSTIISKDLKKRGWTFVGPTTVYAFM
HHHHHHHHHEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHH
QAMGLVNDHLEGCFCRPEVEAMRAVLVRP
HHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SETGIIIGEDGRSRCHWHANLPDYLRYHDEEWGRPVTNDIRLFEKICLEGFQSGLSWLT
CCCCEEEECCCCCEEEEECCCHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
ILRKRENFRAAFSGFDFEKVALFGDQDVTRCLADAGIIRHRGKIVSTINNARRAIALRDE
HHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FGSLAHYFWRYEPGHNERPGVVDREHIVANPTTPTSTIISKDLKKRGWTFVGPTTVYAFM
HHHHHHHHHEECCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEECHHHHHHHH
QAMGLVNDHLEGCFCRPEVEAMRAVLVRP
HHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]