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The map label for this gene is potH [H]

Identifier: 209547985

GI number: 209547985

Start: 385652

End: 386563

Strand: Direct

Name: potH [H]

Synonym: Rleg2_0377

Alternate gene names: 209547985

Gene position: 385652-386563 (Clockwise)

Preceding gene: 209547984

Following gene: 209547986

Centisome position: 8.5

GC content: 59.76

Gene sequence:

>912_bases
ATGGGCAAGCTCACATCCGGCCTCTACAACCGGCTCGTCATCACCATCCCTTATGCCTGGCTGCTGCTCTTCTTCCTGGC
GCCGTTCTTCATCGTCTTCCGCATTTCGCTGTCGACGACGGCAATCGCCATGCCGCCTTACGAGCCGGTTTTCTCGCTTG
CCGACGGCTGGGCCGGGCTCTGGAGCAAGATCGGAGAGCTGTCTTTCGACAATTACGCCTACCTCACCGACGACGCGCTC
TATTTCAATGCCTATATCTCGAGCGTGGTAATTGCCGGGATCTCCACCTTCCTCACATTGCTGATCGCCTATCCGATCGC
CTATGGCATGGCGCAGGCGCCGCGCACCATCCGCCCGACGCTGGTGATGCTGGTCATCCTGCCGTTCTGGACGAGCTTCC
TGATCCGCGTCTATTCCTGGATCGCGATCCTGAAGCCGGAAGGGCTTCTCAACCAGCTTCTCCAGTCGCTGCACATCATC
GACAGCCCGCTGATCATCCTCAACACCACCACCGCCGTCTATATCGGCATCGTCTATTCCTATCTGCCGTTCATGGTCCT
GCCGCTCTATTCAGCGCTGGAAAAGATGGACGGCACGCTGATCGAGGCTGCACAGGATCTCGGTTGCACCCCGATCAGGG
CCTTCTGGCGTGTCACCTTTCCGCTGTCGATCCCCGGTGTGGTGGCCGGCTGCATGCTGGTCTTCATTCCCGCTGTCGGT
GAATTCGTCATTCCCGACCTGCTCGGCGGTTCGCAGACGCTGATGATCGGCAAGACGCTCTGGAACGAGTTCAACGCCAA
CCGCGACTGGCCGGTCTCCTCCGCGGTGGCGACGATCCTGCTGATGATCCTGGTGATCCCGATCGTCTTCTTCCAGAACG
CCCAGGCCAAGGCCGAAGAGCGGGGGAAATAA

Upstream 100 bases:

>100_bases
CTCAATGCGCAACGCTCCGTCGACGACCCCTTCACCTACGACCAGGAAGTCTGGATCTCGTTCGACGAGAACGCCGGCGT
TCTCTTGAAGGATTGAGATC

Downstream 100 bases:

>100_bases
GCCATGCTGAAATGGACCCGCTTCAACATCGTCTCCGTCACGCTCGGCTTTGCCTTCCTTTACCTGCCGATCGTGCTTCT
GGTGATCTTCTCCTTCAACG

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGKLTSGLYNRLVITIPYAWLLLFFLAPFFIVFRISLSTTAIAMPPYEPVFSLADGWAGLWSKIGELSFDNYAYLTDDAL
YFNAYISSVVIAGISTFLTLLIAYPIAYGMAQAPRTIRPTLVMLVILPFWTSFLIRVYSWIAILKPEGLLNQLLQSLHII
DSPLIILNTTTAVYIGIVYSYLPFMVLPLYSALEKMDGTLIEAAQDLGCTPIRAFWRVTFPLSIPGVVAGCMLVFIPAVG
EFVIPDLLGGSQTLMIGKTLWNEFNANRDWPVSSAVATILLMILVIPIVFFQNAQAKAEERGK

Sequences:

>Translated_303_residues
MGKLTSGLYNRLVITIPYAWLLLFFLAPFFIVFRISLSTTAIAMPPYEPVFSLADGWAGLWSKIGELSFDNYAYLTDDAL
YFNAYISSVVIAGISTFLTLLIAYPIAYGMAQAPRTIRPTLVMLVILPFWTSFLIRVYSWIAILKPEGLLNQLLQSLHII
DSPLIILNTTTAVYIGIVYSYLPFMVLPLYSALEKMDGTLIEAAQDLGCTPIRAFWRVTFPLSIPGVVAGCMLVFIPAVG
EFVIPDLLGGSQTLMIGKTLWNEFNANRDWPVSSAVATILLMILVIPIVFFQNAQAKAEERGK
>Mature_302_residues
GKLTSGLYNRLVITIPYAWLLLFFLAPFFIVFRISLSTTAIAMPPYEPVFSLADGWAGLWSKIGELSFDNYAYLTDDALY
FNAYISSVVIAGISTFLTLLIAYPIAYGMAQAPRTIRPTLVMLVILPFWTSFLIRVYSWIAILKPEGLLNQLLQSLHIID
SPLIILNTTTAVYIGIVYSYLPFMVLPLYSALEKMDGTLIEAAQDLGCTPIRAFWRVTFPLSIPGVVAGCMLVFIPAVGE
FVIPDLLGGSQTLMIGKTLWNEFNANRDWPVSSAVATILLMILVIPIVFFQNAQAKAEERGK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=294, Percent_Identity=54.7619047619048, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=41.4529914529915, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=27.5720164609054, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33641; Mature: 33510

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLTSGLYNRLVITIPYAWLLLFFLAPFFIVFRISLSTTAIAMPPYEPVFSLADGWAGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
WSKIGELSFDNYAYLTDDALYFNAYISSVVIAGISTFLTLLIAYPIAYGMAQAPRTIRPT
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVMLVILPFWTSFLIRVYSWIAILKPEGLLNQLLQSLHIIDSPLIILNTTTAVYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHH
YLPFMVLPLYSALEKMDGTLIEAAQDLGCTPIRAFWRVTFPLSIPGVVAGCMLVFIPAVG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTLWNEFNANRDWPVSSAVATILLMILVIPIVFFQNAQAKAEE
HHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RGK
CCC
>Mature Secondary Structure 
GKLTSGLYNRLVITIPYAWLLLFFLAPFFIVFRISLSTTAIAMPPYEPVFSLADGWAGL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHH
WSKIGELSFDNYAYLTDDALYFNAYISSVVIAGISTFLTLLIAYPIAYGMAQAPRTIRPT
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LVMLVILPFWTSFLIRVYSWIAILKPEGLLNQLLQSLHIIDSPLIILNTTTAVYIGIVYS
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHH
YLPFMVLPLYSALEKMDGTLIEAAQDLGCTPIRAFWRVTFPLSIPGVVAGCMLVFIPAVG
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHH
EFVIPDLLGGSQTLMIGKTLWNEFNANRDWPVSSAVATILLMILVIPIVFFQNAQAKAEE
HHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RGK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]