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The map label for this gene is potF [H]

Identifier: 209547983

GI number: 209547983

Start: 383318

End: 384415

Strand: Direct

Name: potF [H]

Synonym: Rleg2_0375

Alternate gene names: 209547983

Gene position: 383318-384415 (Clockwise)

Preceding gene: 209547982

Following gene: 209547984

Centisome position: 8.45

GC content: 59.84

Gene sequence:

>1098_bases
ATGAGGTCAATCATTGCAAGTGTGACGGCCGCCGCCGTTGCCGCGCTGCTTACCGCCGCGCCGGCCTTTGCGCAGGAGCG
CGTGGTCAACGTCTACAACTGGTCGGATTATATCGACGACAGCATTCTTGCCGACTTCACCAAGGAAACCGGCATCAAAG
TCGTCTACGACACCTTCGATTCCAACGAGACCGTGGAAACCAAGCTGCTGGCCGGCGGCACCGGTTATGACGTCGTCGTT
CCCACAGCCGACTTCCTGCAGCGCCAGATCCAGGCCGGCGTCTTCCAGAAGCTCGACAAGTCGAAACTGCCGAACCTCTC
CAACATGTGGGATGTGATCCAGCAGCGCACCGCCGAATACGACCCGGGCAACGAACATGCGGTCGATTACATGTGGGGCA
CCGACGGCATCGGCTACAACGTCAAGAAGGTCGCCGAAATCCTCGGTCCCGATGCCAAGCCCGGCCTCGAAGTGATCTTC
GATCCGAAGGTCGCCGCAAAGTTCAAGGATTGCGGCATCTATATTCTCGACACACCGAAGGACGTCATTACCACGGCGTT
TCGCTATCTCGGCCTCGACCCGAACTCCACCAAGGCCGAGGATTTCAAGAAGGCCGAAGAGCTGCTGACGGCCGCCCGCC
CCTATGTCCGCAAGTTCCATTCGTCCGAATACATCAATGCGCTTGCCAACGGCGACATCTGCATCGCCTTCGGCTATTCC
GGAGACATGCTGCAGGCGCGCGACCGTGCGGCCGAAGCCAAGAACGGCGTCGAGGTCAATTATTCGGTTCCCCCGCAGGG
CGCCCAGATGTGGTTCGACATGATGGCCATCCCCGCCGATGCGCCCCACGTCGCCGAAGCCCACGAATTCCTCAACTACA
TGATGAAGCCCGAGGTCATCGCCAAGGCGAGCGATCACACCTTCTATGCCAACGGCAACAAGGCCTCGCAGCAGTTCGTC
AGCAAGGACATTCTGGAAGACCCTGCCGTCTATCCGACCGAGGCGGTGATGAAGAACCTCTTCACGGTCAAGCCGTGGGA
TCCGAAAACGCAGCGCCTGGGGACGCGCCTCTGGACGAAGGTCGTTACCGGCCAGTAA

Upstream 100 bases:

>100_bases
TTTGCGCTTGTGGGCTGTGAAATTCCTTCTTAGTCTGCTGGCAAAACCAGAGGGAAAAGACGTCCGGGGGTGCGGGCTCT
TTGAACAAACAGGAGAAGTC

Downstream 100 bases:

>100_bases
TGCAGCCTTGGGCCCGGACGGAAACGTCCGGGCCTTTCAATTACCGGCCCGGGTGGAGGCCGGAAAAAACCTTGTATTCG
GGGAATAATATGAAGTCTCT

Product: family 1 extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MRSIIASVTAAAVAALLTAAPAFAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDTFDSNETVETKLLAGGTGYDVVV
PTADFLQRQIQAGVFQKLDKSKLPNLSNMWDVIQQRTAEYDPGNEHAVDYMWGTDGIGYNVKKVAEILGPDAKPGLEVIF
DPKVAAKFKDCGIYILDTPKDVITTAFRYLGLDPNSTKAEDFKKAEELLTAARPYVRKFHSSEYINALANGDICIAFGYS
GDMLQARDRAAEAKNGVEVNYSVPPQGAQMWFDMMAIPADAPHVAEAHEFLNYMMKPEVIAKASDHTFYANGNKASQQFV
SKDILEDPAVYPTEAVMKNLFTVKPWDPKTQRLGTRLWTKVVTGQ

Sequences:

>Translated_365_residues
MRSIIASVTAAAVAALLTAAPAFAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDTFDSNETVETKLLAGGTGYDVVV
PTADFLQRQIQAGVFQKLDKSKLPNLSNMWDVIQQRTAEYDPGNEHAVDYMWGTDGIGYNVKKVAEILGPDAKPGLEVIF
DPKVAAKFKDCGIYILDTPKDVITTAFRYLGLDPNSTKAEDFKKAEELLTAARPYVRKFHSSEYINALANGDICIAFGYS
GDMLQARDRAAEAKNGVEVNYSVPPQGAQMWFDMMAIPADAPHVAEAHEFLNYMMKPEVIAKASDHTFYANGNKASQQFV
SKDILEDPAVYPTEAVMKNLFTVKPWDPKTQRLGTRLWTKVVTGQ
>Mature_365_residues
MRSIIASVTAAAVAALLTAAPAFAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDTFDSNETVETKLLAGGTGYDVVV
PTADFLQRQIQAGVFQKLDKSKLPNLSNMWDVIQQRTAEYDPGNEHAVDYMWGTDGIGYNVKKVAEILGPDAKPGLEVIF
DPKVAAKFKDCGIYILDTPKDVITTAFRYLGLDPNSTKAEDFKKAEELLTAARPYVRKFHSSEYINALANGDICIAFGYS
GDMLQARDRAAEAKNGVEVNYSVPPQGAQMWFDMMAIPADAPHVAEAHEFLNYMMKPEVIAKASDHTFYANGNKASQQFV
SKDILEDPAVYPTEAVMKNLFTVKPWDPKTQRLGTRLWTKVVTGQ

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=363, Percent_Identity=51.5151515151515, Blast_Score=383, Evalue=1e-108,
Organism=Escherichia coli, GI1787367, Length=317, Percent_Identity=30.2839116719243, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40394; Mature: 40394

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIASVTAAAVAALLTAAPAFAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDTFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCEEEEEECC
SNETVETKLLAGGTGYDVVVPTADFLQRQIQAGVFQKLDKSKLPNLSNMWDVIQQRTAEY
CCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPGNEHAVDYMWGTDGIGYNVKKVAEILGPDAKPGLEVIFDPKVAAKFKDCGIYILDTPK
CCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCEEEEECCH
DVITTAFRYLGLDPNSTKAEDFKKAEELLTAARPYVRKFHSSEYINALANGDICIAFGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCC
GDMLQARDRAAEAKNGVEVNYSVPPQGAQMWFDMMAIPADAPHVAEAHEFLNYMMKPEVI
CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
AKASDHTFYANGNKASQQFVSKDILEDPAVYPTEAVMKNLFTVKPWDPKTQRLGTRLWTK
EECCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VVTGQ
HHCCC
>Mature Secondary Structure
MRSIIASVTAAAVAALLTAAPAFAQERVVNVYNWSDYIDDSILADFTKETGIKVVYDTFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCEEEEEECC
SNETVETKLLAGGTGYDVVVPTADFLQRQIQAGVFQKLDKSKLPNLSNMWDVIQQRTAEY
CCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPGNEHAVDYMWGTDGIGYNVKKVAEILGPDAKPGLEVIFDPKVAAKFKDCGIYILDTPK
CCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHCCEEEEECCH
DVITTAFRYLGLDPNSTKAEDFKKAEELLTAARPYVRKFHSSEYINALANGDICIAFGYS
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEECCC
GDMLQARDRAAEAKNGVEVNYSVPPQGAQMWFDMMAIPADAPHVAEAHEFLNYMMKPEVI
CCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHH
AKASDHTFYANGNKASQQFVSKDILEDPAVYPTEAVMKNLFTVKPWDPKTQRLGTRLWTK
EECCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
VVTGQ
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]