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The map label for this gene is aceA [H]

Identifier: 209547981

GI number: 209547981

Start: 380300

End: 381589

Strand: Reverse

Name: aceA [H]

Synonym: Rleg2_0373

Alternate gene names: 209547981

Gene position: 381589-380300 (Counterclockwise)

Preceding gene: 209547987

Following gene: 209547980

Centisome position: 8.41

GC content: 63.18

Gene sequence:

>1290_bases
ATGACCGATTTTTACAAACTTGTTCCCAATGCACCGACAGGCCGCTTCGACGGCATCGACAGGCCCTATTCGGCCGATGA
CGTGCGGCGGCTGAGGGGCTCGGTGGCGCTGACCCATACGCTCGCCGAAATGGGGGCGGACCGGTTGTGGCGGCTGCTGC
GCCAGGAGGATTTCGTCAATGCGCTCGGCGCGCTTTCCGGCAACCAGGCGATGCAGATGGTGCGCGCCGGACTGAAGGCG
ATCTATCTCTCCGGCTGGCAGGTGGCGGCCGACGCCAATACGGCATCGGCGATGTATCCCGATCAGTCGCTCTACCCCGC
CAATGCCGGGCCGGAGCTTGCCAAGCGCATCAATCGCACGCTGCAGCGCGCCGATCAGATCGAGACCTCGGAGGGTCAGG
GGCTTTCCGTCGACAGCTGGTTCGCGCCAATCGTCGCCGATGCGGAAGCCGGCTTCGGCGGGCCGCTCAACGCCTTCGAG
ATCATGAAGGCCTATATCGAGGCGGGTGCGGCCGGCGTCCACTTCGAGGATCAGCTTGCCTCGGAAAAGAAATGCGGCCA
TCTCGGCGGCAAGGTATTGATCCCGACGGCCGCCCATATCCGCAATCTCAACGCCGCCCGGCTTGCCGCCGACGTCATGG
GCGTTGCGACGCTGGTGATTGCCCGCACCGACGCGGAAGCGGCAAAGCTGCTGACATCCGATATCGACGAACGCGACCAG
CCCTTCGTTGATTATGATGCCGGGCGCACCGTCGAAGGCTTCTATCAGGTCAGGAACGGCATCGAACCCTGCATCGCCCG
GGCCGTTGCCTATGCACCCTATTGCGATCTGATCTGGTGCGAGACCTCGAAGCCCGACCTCGATCAGGCGCGGCGGTTTG
CCGAAGGCGTGCACAGGGTCCATCCCGGCAAGCTGCTCGCCTATAATTGCTCGCCGTCCTTCAACTGGAAGAAAAACCTC
GACGAGGCGACGATCGCAAAGTTCCAGCGCGAACTCGGCGCGATGGGCTACAAGTTCCAGTTCATCACGCTCGCCGGCTT
CCACCAGCTGAATTACGGCATGTACGAGCTGGCGCGCGGCTACCGGCAGCGGCAGATGTCGGCCTATTCGGAGCTGCAGC
AGGCGGAATTCGACGCCGAGGTCAACGGCTATACCGCCACCAAACATCAGCGCGAGGTCGGCACCGGCTATTTCGACGCG
GTGTCGCTTGCCATCACCGGCGGCCGGTCGTCGACCACGGCAATGCACGATTCAACCGAACATGCGCAGTTCAAACCGGC
TGCGGAATAG

Upstream 100 bases:

>100_bases
TTTCCTAGCCGGAAATCAAAGGTTTTCCTGGCCATGCCGGGCGATATTGTCAATCTTGTCATCAACACCCGCGATATCGA
TTTTTAGTGAGGAGATCAGC

Downstream 100 bases:

>100_bases
AGCGAAAGAGGAGGAGAAAATCATGGCATCCATTTCACGCGTCAGGGAACGGGCCGAGGAGCAGGCGACCAGCATGAGCG
AGGATCAGCAGACGACGATC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTDFYKLVPNAPTGRFDGIDRPYSADDVRRLRGSVALTHTLAEMGADRLWRLLRQEDFVNALGALSGNQAMQMVRAGLKA
IYLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRTLQRADQIETSEGQGLSVDSWFAPIVADAEAGFGGPLNAFE
IMKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLNAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQ
PFVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPYCDLIWCETSKPDLDQARRFAEGVHRVHPGKLLAYNCSPSFNWKKNL
DEATIAKFQRELGAMGYKFQFITLAGFHQLNYGMYELARGYRQRQMSAYSELQQAEFDAEVNGYTATKHQREVGTGYFDA
VSLAITGGRSSTTAMHDSTEHAQFKPAAE

Sequences:

>Translated_429_residues
MTDFYKLVPNAPTGRFDGIDRPYSADDVRRLRGSVALTHTLAEMGADRLWRLLRQEDFVNALGALSGNQAMQMVRAGLKA
IYLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRTLQRADQIETSEGQGLSVDSWFAPIVADAEAGFGGPLNAFE
IMKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLNAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQ
PFVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPYCDLIWCETSKPDLDQARRFAEGVHRVHPGKLLAYNCSPSFNWKKNL
DEATIAKFQRELGAMGYKFQFITLAGFHQLNYGMYELARGYRQRQMSAYSELQQAEFDAEVNGYTATKHQREVGTGYFDA
VSLAITGGRSSTTAMHDSTEHAQFKPAAE
>Mature_428_residues
TDFYKLVPNAPTGRFDGIDRPYSADDVRRLRGSVALTHTLAEMGADRLWRLLRQEDFVNALGALSGNQAMQMVRAGLKAI
YLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRTLQRADQIETSEGQGLSVDSWFAPIVADAEAGFGGPLNAFEI
MKAYIEAGAAGVHFEDQLASEKKCGHLGGKVLIPTAAHIRNLNAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQP
FVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPYCDLIWCETSKPDLDQARRFAEGVHRVHPGKLLAYNCSPSFNWKKNLD
EATIAKFQRELGAMGYKFQFITLAGFHQLNYGMYELARGYRQRQMSAYSELQQAEFDAEVNGYTATKHQREVGTGYFDAV
SLAITGGRSSTTAMHDSTEHAQFKPAAE

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=419, Percent_Identity=61.8138424821002, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1786525, Length=194, Percent_Identity=32.4742268041237, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17561814, Length=428, Percent_Identity=68.2242990654206, Blast_Score=617, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI71982926, Length=428, Percent_Identity=68.2242990654206, Blast_Score=617, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6325263, Length=243, Percent_Identity=38.6831275720165, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6320908, Length=260, Percent_Identity=35, Blast_Score=154, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47087; Mature: 46956

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFYKLVPNAPTGRFDGIDRPYSADDVRRLRGSVALTHTLAEMGADRLWRLLRQEDFVN
CCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRT
HHHHCCCHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LQRADQIETSEGQGLSVDSWFAPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLA
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SEKKCGHLGGKVLIPTAAHIRNLNAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQ
HHHHHHHCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
PFVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPYCDLIWCETSKPDLDQARRFAEGVHRV
CCEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHC
HPGKLLAYNCSPSFNWKKNLDEATIAKFQRELGAMGYKFQFITLAGFHQLNYGMYELARG
CCCCEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHCCCHHHHHHH
YRQRQMSAYSELQQAEFDAEVNGYTATKHQREVGTGYFDAVSLAITGGRSSTTAMHDSTE
HHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCC
HAQFKPAAE
CCCCCCCCC
>Mature Secondary Structure 
TDFYKLVPNAPTGRFDGIDRPYSADDVRRLRGSVALTHTLAEMGADRLWRLLRQEDFVN
CCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRT
HHHHCCCHHHHHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LQRADQIETSEGQGLSVDSWFAPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLA
HHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
SEKKCGHLGGKVLIPTAAHIRNLNAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQ
HHHHHHHCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
PFVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPYCDLIWCETSKPDLDQARRFAEGVHRV
CCEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHC
HPGKLLAYNCSPSFNWKKNLDEATIAKFQRELGAMGYKFQFITLAGFHQLNYGMYELARG
CCCCEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCEEEEEEEEECHHHHCCCHHHHHHH
YRQRQMSAYSELQQAEFDAEVNGYTATKHQREVGTGYFDAVSLAITGGRSSTTAMHDSTE
HHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHCCCHHHEEEEEEECCCCCCCCCCCCCC
HAQFKPAAE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]