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The map label for this gene is aglA [H]

Identifier: 209547966

GI number: 209547966

Start: 361945

End: 363597

Strand: Direct

Name: aglA [H]

Synonym: Rleg2_0358

Alternate gene names: 209547966

Gene position: 361945-363597 (Clockwise)

Preceding gene: 209547965

Following gene: 209547967

Centisome position: 7.98

GC content: 62.13

Gene sequence:

>1653_bases
ATGAACGTGGCTTCCCAATCGATCTCGACCCCCGATAAGGACTGGTGGCGCGGCGCGGTGATCTATCAGATCTACCCGCG
CTCCTACCAGGATTCGAACGGCGACGGCATCGGCGACCTGAAGGGCATCACCGCCCGCCTGCCGCATGTGGCAAGCCTCG
GCGTCGATGCGATCTGGATCTCGCCCTTCTTCACCTCGCCGATGCGCGATTTCGGTTACGACGTTTCCGACTACGAAAAT
GTCGATTCGATCTTCGGCACGCTGGTGGATTTCGACACGATGATCGCTGAGGCCCATCGCCTCGGCATCCGCGTGATGAT
CGACCTTGTCATCTCGCACAGCTCGGATCAGCACCCCTGGTTCGTGCAAAGCCGCTCCAGCAAGACCAACGCCAAGGCCG
ATTGGTATGTCTGGGCCGATGCCAAGCCGGACGGTACGCCGCCGAACAACTGGCTGTCGATCTTCGGCGGCTCGGCATGG
GCGTGGGATCCGACGCGCATGCAATATTACCTGCACAACTTCCTGACCTCGCAGCCGGATATGAACCTGCATAATCCCGA
GGTGCAGGACCGTCTGCTCGATGTCGTGCGCTTCTGGCTCAACCGCGGCGTCGACGGCTTCCGCCTCGACACCATCAATT
TCTATTTCCACGACCCGCTGTTGCGCGACAATCCGGCACTTGCGCCTGAGCGCCGCAACGCCTCGACGGCGCCGGCGGTC
AATCCCTATAATTTCCAGGAGCATGTCTACGACAAGAACCGGCCGGAGAACCTTGCCTTCCTGAAGCGCTTCCGCGCCGT
TCTGGAAGAATTCCCGGCGATTGCCGCCGTCGGCGAAGTCGGCGACAGCCAGCGCGGCCTCGAAATCGTCGGCGAATATA
CCTCCGGCAACGACAAGATGCATATGTGCTATGCCTTCGAATTCCTGGCGCCCGATCCGCTGACGGCCGAGCGCGTCGAA
GAGGTGATGCAGGATTTCGAAGCCGCAGCACCGGATGGCTGGGCCTGCTGGGCCTTCTCCAATCACGACGTCATGCGCCA
TGTCAGCCGCTGGGGCGGGCTGGTCGCCGATCATGACGCCTTCGCCAAGCTCTATGCCTCGCTGCTCCTGACGCTGCGCG
GCTCGGTCTGCCTCTATCAGGGCGAGGAGCTGGCGCTGACCGAAGCCGATCTCGCCTATCAGGATCTGCAGGATCCCTAC
GGCATCCAGTTCTGGCCGGAGTTCAAGGGCCGCGACGGCTGCCGCACGCCGATGGTCTGGGACAGCCAGGTCGCCCAGGG
CGGCTTTTCCACGGTCAAGCCCTGGCTGCCGGTGCCGGTCGAGCATATTCTGCGCGCCGTCAGCGTCCAGCAGGGCGACG
AGGCTTCGGTGCTGGAGCACTATCGCCGTTTCATCGCTTTCCGCAAATTGCACCCGGCCTTTGCCAAGGGCGAGATCGAA
TTCGAGGAGCCGCAGGGCGACGCCCTGGTCTTCACCCGTGAATACGGCAACGAGAAGCTGCTCTGCATCTTCAACATGAG
CCCGGCTGAAACCGGCGTCACTTTGCCCGGCGGCGAATGGCAGGCGTTGACGGGGCATGGCTTTATCAGCAACAACTATG
GCGACAAGATCGATATTCCGGCCTGGGGGGCGTATTTCGCCCGTCTCGCTTAA

Upstream 100 bases:

>100_bases
GTCTTCTTCGGGCTGCAGCGTTATCTGGTGCGCGGCCTGCTGGCGGGTTCGGTCAAGGGAGGCTGACCATTTCCAAACGT
GACTTCAGACAGGATATTCC

Downstream 100 bases:

>100_bases
GGATCAGCAGGGGAGAGAGACATGACTGGACTGACGCTGAAGGATATCCGCAAATCCTACGGTTCCGTGGACGTTCTCCA
CGGGATCGACCTCGATATCA

Product: alpha amylase catalytic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MNVASQSISTPDKDWWRGAVIYQIYPRSYQDSNGDGIGDLKGITARLPHVASLGVDAIWISPFFTSPMRDFGYDVSDYEN
VDSIFGTLVDFDTMIAEAHRLGIRVMIDLVISHSSDQHPWFVQSRSSKTNAKADWYVWADAKPDGTPPNNWLSIFGGSAW
AWDPTRMQYYLHNFLTSQPDMNLHNPEVQDRLLDVVRFWLNRGVDGFRLDTINFYFHDPLLRDNPALAPERRNASTAPAV
NPYNFQEHVYDKNRPENLAFLKRFRAVLEEFPAIAAVGEVGDSQRGLEIVGEYTSGNDKMHMCYAFEFLAPDPLTAERVE
EVMQDFEAAAPDGWACWAFSNHDVMRHVSRWGGLVADHDAFAKLYASLLLTLRGSVCLYQGEELALTEADLAYQDLQDPY
GIQFWPEFKGRDGCRTPMVWDSQVAQGGFSTVKPWLPVPVEHILRAVSVQQGDEASVLEHYRRFIAFRKLHPAFAKGEIE
FEEPQGDALVFTREYGNEKLLCIFNMSPAETGVTLPGGEWQALTGHGFISNNYGDKIDIPAWGAYFARLA

Sequences:

>Translated_550_residues
MNVASQSISTPDKDWWRGAVIYQIYPRSYQDSNGDGIGDLKGITARLPHVASLGVDAIWISPFFTSPMRDFGYDVSDYEN
VDSIFGTLVDFDTMIAEAHRLGIRVMIDLVISHSSDQHPWFVQSRSSKTNAKADWYVWADAKPDGTPPNNWLSIFGGSAW
AWDPTRMQYYLHNFLTSQPDMNLHNPEVQDRLLDVVRFWLNRGVDGFRLDTINFYFHDPLLRDNPALAPERRNASTAPAV
NPYNFQEHVYDKNRPENLAFLKRFRAVLEEFPAIAAVGEVGDSQRGLEIVGEYTSGNDKMHMCYAFEFLAPDPLTAERVE
EVMQDFEAAAPDGWACWAFSNHDVMRHVSRWGGLVADHDAFAKLYASLLLTLRGSVCLYQGEELALTEADLAYQDLQDPY
GIQFWPEFKGRDGCRTPMVWDSQVAQGGFSTVKPWLPVPVEHILRAVSVQQGDEASVLEHYRRFIAFRKLHPAFAKGEIE
FEEPQGDALVFTREYGNEKLLCIFNMSPAETGVTLPGGEWQALTGHGFISNNYGDKIDIPAWGAYFARLA
>Mature_550_residues
MNVASQSISTPDKDWWRGAVIYQIYPRSYQDSNGDGIGDLKGITARLPHVASLGVDAIWISPFFTSPMRDFGYDVSDYEN
VDSIFGTLVDFDTMIAEAHRLGIRVMIDLVISHSSDQHPWFVQSRSSKTNAKADWYVWADAKPDGTPPNNWLSIFGGSAW
AWDPTRMQYYLHNFLTSQPDMNLHNPEVQDRLLDVVRFWLNRGVDGFRLDTINFYFHDPLLRDNPALAPERRNASTAPAV
NPYNFQEHVYDKNRPENLAFLKRFRAVLEEFPAIAAVGEVGDSQRGLEIVGEYTSGNDKMHMCYAFEFLAPDPLTAERVE
EVMQDFEAAAPDGWACWAFSNHDVMRHVSRWGGLVADHDAFAKLYASLLLTLRGSVCLYQGEELALTEADLAYQDLQDPY
GIQFWPEFKGRDGCRTPMVWDSQVAQGGFSTVKPWLPVPVEHILRAVSVQQGDEASVLEHYRRFIAFRKLHPAFAKGEIE
FEEPQGDALVFTREYGNEKLLCIFNMSPAETGVTLPGGEWQALTGHGFISNNYGDKIDIPAWGAYFARLA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=535, Percent_Identity=31.0280373831776, Blast_Score=265, Evalue=9e-71,
Organism=Escherichia coli, GI1790687, Length=523, Percent_Identity=34.225621414914, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1786604, Length=518, Percent_Identity=22.972972972973, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32565753, Length=496, Percent_Identity=25, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI25147709, Length=504, Percent_Identity=22.6190476190476, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322245, Length=579, Percent_Identity=32.8151986183074, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6321731, Length=591, Percent_Identity=33.3333333333333, Blast_Score=278, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6319776, Length=591, Percent_Identity=33.3333333333333, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321726, Length=567, Percent_Identity=32.6278659611993, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324416, Length=574, Percent_Identity=31.5331010452962, Blast_Score=263, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322241, Length=569, Percent_Identity=32.8646748681898, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322021, Length=569, Percent_Identity=32.8646748681898, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24583747, Length=522, Percent_Identity=36.7816091954023, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI24583749, Length=522, Percent_Identity=36.7816091954023, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24583745, Length=513, Percent_Identity=37.8167641325536, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24586589, Length=521, Percent_Identity=35.1247600767754, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24586591, Length=517, Percent_Identity=34.816247582205, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI45549022, Length=526, Percent_Identity=33.4600760456274, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24586599, Length=529, Percent_Identity=32.703213610586, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24586597, Length=523, Percent_Identity=33.0783938814532, Blast_Score=292, Evalue=5e-79,
Organism=Drosophila melanogaster, GI24586587, Length=520, Percent_Identity=33.4615384615385, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24586593, Length=506, Percent_Identity=34.3873517786561, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI221330053, Length=510, Percent_Identity=34.1176470588235, Blast_Score=285, Evalue=8e-77,
Organism=Drosophila melanogaster, GI281360393, Length=450, Percent_Identity=31.1111111111111, Blast_Score=208, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 62206; Mature: 62206

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVASQSISTPDKDWWRGAVIYQIYPRSYQDSNGDGIGDLKGITARLPHVASLGVDAIWI
CCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHCHHHHHCCHHHHCCCCEEEE
SPFFTSPMRDFGYDVSDYENVDSIFGTLVDFDTMIAEAHRLGIRVMIDLVISHSSDQHPW
CCHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCCE
FVQSRSSKTNAKADWYVWADAKPDGTPPNNWLSIFGGSAWAWDPTRMQYYLHNFLTSQPD
EEECCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCCCC
MNLHNPEVQDRLLDVVRFWLNRGVDGFRLDTINFYFHDPLLRDNPALAPERRNASTAPAV
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NPYNFQEHVYDKNRPENLAFLKRFRAVLEEFPAIAAVGEVGDSQRGLEIVGEYTSGNDKM
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCEEEEEEECCCCCCE
HMCYAFEFLAPDPLTAERVEEVMQDFEAAAPDGWACWAFSNHDVMRHVSRWGGLVADHDA
EEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCEEECHHH
FAKLYASLLLTLRGSVCLYQGEELALTEADLAYQDLQDPYGIQFWPEFKGRDGCRTPMVW
HHHHHHHHHHHHCCCEEEEECCEEEEEHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEE
DSQVAQGGFSTVKPWLPVPVEHILRAVSVQQGDEASVLEHYRRFIAFRKLHPAFAKGEIE
CHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEE
FEEPQGDALVFTREYGNEKLLCIFNMSPAETGVTLPGGEWQALTGHGFISNNYGDKIDIP
EECCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCCCEECCC
AWGAYFARLA
CHHHHHHHCC
>Mature Secondary Structure
MNVASQSISTPDKDWWRGAVIYQIYPRSYQDSNGDGIGDLKGITARLPHVASLGVDAIWI
CCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCHHCHHHHHCCHHHHCCCCEEEE
SPFFTSPMRDFGYDVSDYENVDSIFGTLVDFDTMIAEAHRLGIRVMIDLVISHSSDQHPW
CCHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEHHHHCCCCCCCE
FVQSRSSKTNAKADWYVWADAKPDGTPPNNWLSIFGGSAWAWDPTRMQYYLHNFLTSQPD
EEECCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCEECCCHHHHHHHHHHHHCCCCC
MNLHNPEVQDRLLDVVRFWLNRGVDGFRLDTINFYFHDPLLRDNPALAPERRNASTAPAV
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
NPYNFQEHVYDKNRPENLAFLKRFRAVLEEFPAIAAVGEVGDSQRGLEIVGEYTSGNDKM
CCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEHHCCCCCCCCEEEEEEECCCCCCE
HMCYAFEFLAPDPLTAERVEEVMQDFEAAAPDGWACWAFSNHDVMRHVSRWGGLVADHDA
EEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCEEECHHH
FAKLYASLLLTLRGSVCLYQGEELALTEADLAYQDLQDPYGIQFWPEFKGRDGCRTPMVW
HHHHHHHHHHHHCCCEEEEECCEEEEEHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEE
DSQVAQGGFSTVKPWLPVPVEHILRAVSVQQGDEASVLEHYRRFIAFRKLHPAFAKGEIE
CHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHCCEEE
FEEPQGDALVFTREYGNEKLLCIFNMSPAETGVTLPGGEWQALTGHGFISNNYGDKIDIP
EECCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEEECCCCCEECCC
AWGAYFARLA
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]