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The map label for this gene is folD1 [H]

Identifier: 209547961

GI number: 209547961

Start: 355831

End: 356730

Strand: Direct

Name: folD1 [H]

Synonym: Rleg2_0353

Alternate gene names: 209547961

Gene position: 355831-356730 (Clockwise)

Preceding gene: 209547960

Following gene: 209547963

Centisome position: 7.84

GC content: 64.67

Gene sequence:

>900_bases
GTGACGACAGTAATCGACGGCAAGAACGTAGCTGCATCGGTCATTCAGACGGTCAAGAGTGCGACGGCAGCGCTGGAAAA
GAGCAGCGGCGTCACCACCGGTCTTGCGGTCGTCATCGTCGGTGACGATCCGGCAAGTCACGCCTATGTCGGCTCCAAGG
GCCGCATGGCCAAGGAATGCGGCTTCAAATCGGTCCAGCACACACTGCCGGCCGAGACCAGCCAGGAGGAGCTGGCAGCC
CTCGTCGCCACCCTCAACGCCGATCCGTCGATCCACGGCATCCTGGTGCAATTGCCGCTGCCGAAGCCGCTCGACAGCGA
GCCGATCATCCAATCGATCCTGCCGGAAAAGGATGTCGACGGCTTGAGCATTGCCAATGCCGGCAAGCTTGCCATCGGCG
ACCTGAAGACCGGACTGGTCTCCTGCACGCCGGCCGGCGCCATGGTCTTCGTCCGCCGCACCCATGGCGAGGATCTCTCC
GGCCTCAACGCCGTCGTCATCGGCCGCTCCAACCTGTTCGGCAAGCCGATGGCGCAATTGCTGCTCAATGCCAATGCGAC
GGTGACGATCGCGCATTCGAGAACCAAGAACCTCGCCGAGGTCTGCCGCAATGCCGATATTCTGGTCGCCGCCGTCGGCC
GGCCGGAGATGGTCAAGGCCGATTGGGTCAAGCCGGGTGCGACGGTCATCGACGTCGGCATCAACCGCGTGCCAGCCCCC
GAAAGAGGCGAGGGCAAGACCCGGCTCGTCGGCGACGTCGCCTTCGACGAGGTCTTTGCGGTCGCCAGCACCATCACCCC
GGTTCCCGGTGGTGTCGGGCCGATGACCATCGCCATGCTGATGGCCAATACGGTCATCGCCGCCCATCGCGCCGCAGGGC
AGACCCCGCCGCAGTTCTGA

Upstream 100 bases:

>100_bases
TTGATATTGCCTTGCTGCTTTCCGACAGGATAGGGCGTTAGCGGGCATTTGACGCTCGCCGACTATCCATAGATTGGCGC
GAAACAGGGCGGAGAGACGC

Downstream 100 bases:

>100_bases
TTAATGCATGTCGCCCGGAAGTGTGCAGCGGTTTTCCCAGGCAAAGCGCGAAGCGCTTTTGCCGCAAACGACATGCATAA
AACAAAGAGCTAAAGCAAGG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTTVIDGKNVAASVIQTVKSATAALEKSSGVTTGLAVVIVGDDPASHAYVGSKGRMAKECGFKSVQHTLPAETSQEELAA
LVATLNADPSIHGILVQLPLPKPLDSEPIIQSILPEKDVDGLSIANAGKLAIGDLKTGLVSCTPAGAMVFVRRTHGEDLS
GLNAVVIGRSNLFGKPMAQLLLNANATVTIAHSRTKNLAEVCRNADILVAAVGRPEMVKADWVKPGATVIDVGINRVPAP
ERGEGKTRLVGDVAFDEVFAVASTITPVPGGVGPMTIAMLMANTVIAAHRAAGQTPPQF

Sequences:

>Translated_299_residues
MTTVIDGKNVAASVIQTVKSATAALEKSSGVTTGLAVVIVGDDPASHAYVGSKGRMAKECGFKSVQHTLPAETSQEELAA
LVATLNADPSIHGILVQLPLPKPLDSEPIIQSILPEKDVDGLSIANAGKLAIGDLKTGLVSCTPAGAMVFVRRTHGEDLS
GLNAVVIGRSNLFGKPMAQLLLNANATVTIAHSRTKNLAEVCRNADILVAAVGRPEMVKADWVKPGATVIDVGINRVPAP
ERGEGKTRLVGDVAFDEVFAVASTITPVPGGVGPMTIAMLMANTVIAAHRAAGQTPPQF
>Mature_298_residues
TTVIDGKNVAASVIQTVKSATAALEKSSGVTTGLAVVIVGDDPASHAYVGSKGRMAKECGFKSVQHTLPAETSQEELAAL
VATLNADPSIHGILVQLPLPKPLDSEPIIQSILPEKDVDGLSIANAGKLAIGDLKTGLVSCTPAGAMVFVRRTHGEDLSG
LNAVVIGRSNLFGKPMAQLLLNANATVTIAHSRTKNLAEVCRNADILVAAVGRPEMVKADWVKPGATVIDVGINRVPAPE
RGEGKTRLVGDVAFDEVFAVASTITPVPGGVGPMTIAMLMANTVIAAHRAAGQTPPQF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=297, Percent_Identity=46.4646464646465, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=42.7609427609428, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=42.7609427609428, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI36796743, Length=225, Percent_Identity=28.4444444444444, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1786741, Length=285, Percent_Identity=47.719298245614, Blast_Score=237, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=42.4657534246575, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=45.8333333333333, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=41.2162162162162, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=47.2413793103448, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=47.2413793103448, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=47.2413793103448, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=47.2413793103448, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17136818, Length=301, Percent_Identity=46.1794019933555, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17136816, Length=301, Percent_Identity=46.1794019933555, Blast_Score=228, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30813; Mature: 30682

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVIDGKNVAASVIQTVKSATAALEKSSGVTTGLAVVIVGDDPASHAYVGSKGRMAKEC
CCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEECCCCCCHHHH
GFKSVQHTLPAETSQEELAALVATLNADPSIHGILVQLPLPKPLDSEPIIQSILPEKDVD
CHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCC
GLSIANAGKLAIGDLKTGLVSCTPAGAMVFVRRTHGEDLSGLNAVVIGRSNLFGKPMAQL
CCEECCCCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHH
LLNANATVTIAHSRTKNLAEVCRNADILVAAVGRPEMVKADWVKPGATVIDVGINRVPAP
HHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEEEEECCCCCCCC
ERGEGKTRLVGDVAFDEVFAVASTITPVPGGVGPMTIAMLMANTVIAAHRAAGQTPPQF
CCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TTVIDGKNVAASVIQTVKSATAALEKSSGVTTGLAVVIVGDDPASHAYVGSKGRMAKEC
CEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEECCCCCCHHHH
GFKSVQHTLPAETSQEELAALVATLNADPSIHGILVQLPLPKPLDSEPIIQSILPEKDVD
CHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHCCCCCCC
GLSIANAGKLAIGDLKTGLVSCTPAGAMVFVRRTHGEDLSGLNAVVIGRSNLFGKPMAQL
CCEECCCCCEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHH
LLNANATVTIAHSRTKNLAEVCRNADILVAAVGRPEMVKADWVKPGATVIDVGINRVPAP
HHCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEEEEECCCCCCCC
ERGEGKTRLVGDVAFDEVFAVASTITPVPGGVGPMTIAMLMANTVIAAHRAAGQTPPQF
CCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA