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The map label for this gene is mscL [H]

Identifier: 209547834

GI number: 209547834

Start: 231481

End: 231918

Strand: Direct

Name: mscL [H]

Synonym: Rleg2_0225

Alternate gene names: 209547834

Gene position: 231481-231918 (Clockwise)

Preceding gene: 209547832

Following gene: 209547835

Centisome position: 5.1

GC content: 60.73

Gene sequence:

>438_bases
ATGCTCAACGAATTCAAGGCCTTTATCGCCCGTGGCAATGTCATGGATCTTGCCGTCGGCGTCATCATCGGCGGCGCCTT
CGGCGGCATCGTCAAATCTCTGGTCGATGACCTGATCATGCCGATCGTCGGCGCCATTTTCGGCGGCTTCGATTTTTCCA
ATTATTTCCTGCCGCTCTCCTCGGCCGTCAACGCGCCGACACTGGCCGCAGCCCGCGCGCAAGGGGCGGTGTTTGCCTAT
GGCAGCTTCCTCACCGTGCTCATCAACTTCCTGATCCTTGCCTGGATCATCTTCCTGATGGTCAAGGGCGTGAACTATCT
GCGCCTGCAGGTCGAGCGCCAGGAAAAGGCCGCACCGGAAGAACTGCCCCCGCCGCCGGCCGATGTTCAGCTGCTGACGG
AGATTCGCGATCTGCTGGCCACACGTCCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCCAAATAATTGCGATTGCCGATTGATTAATCGCAATGAAGATGTAGCTAATGAAATCCATCGCATACTCTCTGAAATGG
ATCGAAGGGAGACGGATCCG

Downstream 100 bases:

>100_bases
TCGAGGCTGGAGGCTGCCCCTCACCCTAACCCTCTCCCCGTAAACGGGGCGAGGGGACGTGCCCTGCGAAACGTTGGCGA
GGAACGGAGAGGTTGCGACT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MLNEFKAFIARGNVMDLAVGVIIGGAFGGIVKSLVDDLIMPIVGAIFGGFDFSNYFLPLSSAVNAPTLAAARAQGAVFAY
GSFLTVLINFLILAWIIFLMVKGVNYLRLQVERQEKAAPEELPPPPADVQLLTEIRDLLATRPTA

Sequences:

>Translated_145_residues
MLNEFKAFIARGNVMDLAVGVIIGGAFGGIVKSLVDDLIMPIVGAIFGGFDFSNYFLPLSSAVNAPTLAAARAQGAVFAY
GSFLTVLINFLILAWIIFLMVKGVNYLRLQVERQEKAAPEELPPPPADVQLLTEIRDLLATRPTA
>Mature_145_residues
MLNEFKAFIARGNVMDLAVGVIIGGAFGGIVKSLVDDLIMPIVGAIFGGFDFSNYFLPLSSAVNAPTLAAARAQGAVFAY
GSFLTVLINFLILAWIIFLMVKGVNYLRLQVERQEKAAPEELPPPPADVQLLTEIRDLLATRPTA

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=142, Percent_Identity=42.9577464788732, Blast_Score=103, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15597; Mature: 15597

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNEFKAFIARGNVMDLAVGVIIGGAFGGIVKSLVDDLIMPIVGAIFGGFDFSNYFLPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHH
SAVNAPTLAAARAQGAVFAYGSFLTVLINFLILAWIIFLMVKGVNYLRLQVERQEKAAPE
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHCCCC
ELPPPPADVQLLTEIRDLLATRPTA
CCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNEFKAFIARGNVMDLAVGVIIGGAFGGIVKSLVDDLIMPIVGAIFGGFDFSNYFLPLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHH
SAVNAPTLAAARAQGAVFAYGSFLTVLINFLILAWIIFLMVKGVNYLRLQVERQEKAAPE
HHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEHHHHHCCCC
ELPPPPADVQLLTEIRDLLATRPTA
CCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA