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The map label for this gene is cspA [C]

Identifier: 209547826

GI number: 209547826

Start: 220987

End: 221196

Strand: Direct

Name: cspA [C]

Synonym: Rleg2_0217

Alternate gene names: 209547826

Gene position: 220987-221196 (Clockwise)

Preceding gene: 209547825

Following gene: 209547828

Centisome position: 4.87

GC content: 58.57

Gene sequence:

>210_bases
ATGAGCACTGGTACCGTAAAGTGGTTCAACGCAACCAAGGGCTTCGGCTTCATTCAGCCGGATGACGGCGCTGCCGACGT
TTTCGTCCACATCTCGGCCGTTGAACGCGCTGGAATGCGCGATCTCAAGGATGGCCAGAAGCTGTCTTACGAGCTCGTCC
GCGACAACAAGTCCGGCAAGATGTCGGCAGACCGCCTCCAGGCGGCTTAA

Upstream 100 bases:

>100_bases
CGTCGTTCCTGGCGAACTTCCTCCAAAATCGGTTTTCATCGCCGCTCGTGCTTCGGCTCGCGCGGCAATCTTCTATGAAC
GATTCGAAAAGGATATCGTC

Downstream 100 bases:

>100_bases
TCTTGACGCGTCCTCTGGGACGCGTGAAGCTTTGAATATCATCTCCGGATCGTGACCACGGATGTACTGGCACGGTTCGT
TGAGATGGCAGGGAAGGTCG

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 69; Mature: 68

Protein sequence:

>69_residues
MSTGTVKWFNATKGFGFIQPDDGAADVFVHISAVERAGMRDLKDGQKLSYELVRDNKSGKMSADRLQAA

Sequences:

>Translated_69_residues
MSTGTVKWFNATKGFGFIQPDDGAADVFVHISAVERAGMRDLKDGQKLSYELVRDNKSGKMSADRLQAA
>Mature_68_residues
STGTVKWFNATKGFGFIQPDDGAADVFVHISAVERAGMRDLKDGQKLSYELVRDNKSGKMSADRLQAA

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=50, Percent_Identity=62, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1786841, Length=54, Percent_Identity=51.8518518518518, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI2367114, Length=50, Percent_Identity=56, Blast_Score=68, Evalue=1e-13,
Organism=Escherichia coli, GI1787839, Length=50, Percent_Identity=58, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=47.5409836065574, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI1787107, Length=62, Percent_Identity=45.1612903225806, Blast_Score=64, Evalue=3e-12,
Organism=Escherichia coli, GI1787834, Length=48, Percent_Identity=52.0833333333333, Blast_Score=61, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7536; Mature: 7404

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGTVKWFNATKGFGFIQPDDGAADVFVHISAVERAGMRDLKDGQKLSYELVRDNKSGK
CCCCCEEEEECCCCCCEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEEEECCCCCC
MSADRLQAA
CCCCCCCCC
>Mature Secondary Structure 
STGTVKWFNATKGFGFIQPDDGAADVFVHISAVERAGMRDLKDGQKLSYELVRDNKSGK
CCCCEEEEECCCCCCEECCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEEEECCCCCC
MSADRLQAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]