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The map label for this gene is fadJ [H]

Identifier: 209547825

GI number: 209547825

Start: 218302

End: 220515

Strand: Direct

Name: fadJ [H]

Synonym: Rleg2_0216

Alternate gene names: 209547825

Gene position: 218302-220515 (Clockwise)

Preceding gene: 209547824

Following gene: 209547826

Centisome position: 4.81

GC content: 63.28

Gene sequence:

>2214_bases
ATGACCTACATAAATTTCACGCTCGAAACCGACGCCGACGGCATCGCTCTCGTCACTTGGGACATGCCCGGCAAATCGAT
GAACGTCTTCACATCAGAGGTGATGGCCGAACTCGACGCCATCATCGACGCCACCACCGCTGATGCCGCCGTCAAGGGCG
TCGTCTTCACCTCGGGCAAATCCTCCTTCTCCGGCGGTGCCGATCTGTCGATGATCAAGTCGATGTTCGGTTCCTATCAG
GACGAAAAGGAAAAGAGCCCGGAGACGGCGGTGCAGACGCTCTTCGGCCTGGTCGGGCGCATGTCCGGCCTGTTCCGCAA
GCTCGAAACATCGGGCAAGCCCTGGGTTTCCGCCATCAACGGCACCTGCATGGGCGGGGCTTTCGAACTGTCGCTCGCCT
GCCATGGCCGCGTCGCCTCCAATGCCAAGAGTGTCAAGATCGCGCTGCCCGAGGTGAAGGTCGGCATCTTCCCCGGCGCC
GGCGGCACCCAGCGCGTGCCGCGGCTGGCCAATGCCCAGGATGCGCTGCAGATGATGACGACCGGCCAGTCGCTGAGCGG
CTCCCGCGCCAAGGCGATGAACCTCGTGCATCAGGTGGTCGAGCCGGATCAGTTGATCCCCGCCGCCAAGCAGATGATCA
AGGACGGCCTGAAGCCGGTCGCCCCCTGGGACGAGAAGGGCTTCAAGCTGCCGGGCGGCGGCATCTGGACACCGGCCTCG
GCCCAGCTCTGGCCGGCCGCACCCGCAATCCTGCGCCGCGAAACATCCGGCAATTATCCGGCAGCGCTCGCCATTCTGAA
ATGCGTCTATGAAGGCCTGCAGCTGCCGTTCGACACCGGCCTGAAGATCGAGCAGCGTTATTTCACCGAAGTGCTGCAGA
CCCGCGAAGCCTTCTCGATGATCCGCTCGCTGTTCATCTCCATGCAGGAGCTCGGCAAGGGCGCCCGCCGCCCGGCAGGC
ATCCCCAAGACCGAGCTGAAGCATGTCGGTGTCGTCGGCGCCGGTTTTATGGGCGCCTCGATCGCCTATGTCACGGCTGC
CGCCGGTCTGGCGGTGACGCTGATCGACCGCGACATGGAGGCGGCTGCCAAGGGCAAGGCCGTCTGCGAAGGTCTCGTCA
AGGATTCCGTCGGTAAGGGACGGTTTACGCAGGACGAGGCCGCAGCCCTTCTTGCCCGCATCACGCCCTCGGCCAATTAT
GCCGATCTCGCCGCTGCCGGCCTGGTCATCGAGGCGGTGTTCGAGGATCGCGAGGTGAAGAAGGCGGTCATCGAGGCTGT
TGAAGCCGTGCTGCCCGAAGGCGCGATCTTCGCCTCCAATACTTCGACCCTGCCGATATCAGGGCTGGCGAAGAACTCCA
AGCGCCCGGCCGATTTCATCGGCATCCACTTCTTCTCGCCCGTGGAGAAGATGATGCTGACCGAGGTCATCCTCGGCAAG
GCGACCGGCGACAAGGCGCTCGCCGTCGCGCTCGACTATGTCGCGGCGATCAAGAAGACGCCGATCGTCGTCAACGACAC
CCGCGGCTTCTTCGTCAATCGCTGCGTGCTGCGCTACATGTCGGAAAGCTACGACATGCTGATCGAGGGCGTGCCGCCCA
CAATGATCGAGAACGCCGCCAAGATGGCCGGCATGCCGGTCGGTCCCTTGGCGCTGAACGACGAGGTTGCCATCGATCTC
TCGCTGAAGATCCTCAAGGCGACCGTCGCCGATCTCGGCGAGGAGGCGATCGACCCAAGGCATATGGACCTCATTTCCCG
CATGGTGGAAAAGGAGGGCCGCTTCGGCCGCAAGAATTCCAAGGGCTTCTACGATTATCCGCCGAAACCGGCGAAGAAGT
CGCTCTGGCCCGAGCTGAAGACGCTCTACCCGCAGAGACGCGCGGACGAGGTCGACGTCAATGTCCTGAAGCAACGCTTC
CTCGTCACCATCGCGCTCGAAGCCGCCCGCACCGTCGAGGAAGGCATCGTCACCGACCCGCGCGAAGCCGACGTCGGCTC
GATCCTCGGATTCGGCTTCGCGCCCTATACCGGCGGGGCGCTGAGCTACATCGACGGAATGGGCGCGAAGGCCTTCGTGG
AACTGGCGGAGAAGTTAGCGGGGACTTACGGAGATCATTTCAAGCCGACGCCGCTGCTCAAGGATATGGCTGCCAAGGGT
GAGACGTTTTATGGGCGGTTTGATCCCTATGCGGGGGCGAAGGTGGCGGCTTAA

Upstream 100 bases:

>100_bases
CCGTCGCAGACCAAGCATACCGGCGACGTCGTCACCGAAAAGACGAAGACAGTCGACGCGCAGCGCAGCCATTTGCCGGC
CTGATCGGGGAGGAAACCTA

Downstream 100 bases:

>100_bases
CCTGTTTCGCGGTCTTGGTTTTAACGGAACTATCTTTACCGTTGTGCCGTGACCGCCCCCCTCTGCCCTGCCGGGCATCT
CCCCCACAGGTGGGGAGATC

Product: NAD-binding 3-hydroxyacyl-CoA dehydrogenase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 737; Mature: 736

Protein sequence:

>737_residues
MTYINFTLETDADGIALVTWDMPGKSMNVFTSEVMAELDAIIDATTADAAVKGVVFTSGKSSFSGGADLSMIKSMFGSYQ
DEKEKSPETAVQTLFGLVGRMSGLFRKLETSGKPWVSAINGTCMGGAFELSLACHGRVASNAKSVKIALPEVKVGIFPGA
GGTQRVPRLANAQDALQMMTTGQSLSGSRAKAMNLVHQVVEPDQLIPAAKQMIKDGLKPVAPWDEKGFKLPGGGIWTPAS
AQLWPAAPAILRRETSGNYPAALAILKCVYEGLQLPFDTGLKIEQRYFTEVLQTREAFSMIRSLFISMQELGKGARRPAG
IPKTELKHVGVVGAGFMGASIAYVTAAAGLAVTLIDRDMEAAAKGKAVCEGLVKDSVGKGRFTQDEAAALLARITPSANY
ADLAAAGLVIEAVFEDREVKKAVIEAVEAVLPEGAIFASNTSTLPISGLAKNSKRPADFIGIHFFSPVEKMMLTEVILGK
ATGDKALAVALDYVAAIKKTPIVVNDTRGFFVNRCVLRYMSESYDMLIEGVPPTMIENAAKMAGMPVGPLALNDEVAIDL
SLKILKATVADLGEEAIDPRHMDLISRMVEKEGRFGRKNSKGFYDYPPKPAKKSLWPELKTLYPQRRADEVDVNVLKQRF
LVTIALEAARTVEEGIVTDPREADVGSILGFGFAPYTGGALSYIDGMGAKAFVELAEKLAGTYGDHFKPTPLLKDMAAKG
ETFYGRFDPYAGAKVAA

Sequences:

>Translated_737_residues
MTYINFTLETDADGIALVTWDMPGKSMNVFTSEVMAELDAIIDATTADAAVKGVVFTSGKSSFSGGADLSMIKSMFGSYQ
DEKEKSPETAVQTLFGLVGRMSGLFRKLETSGKPWVSAINGTCMGGAFELSLACHGRVASNAKSVKIALPEVKVGIFPGA
GGTQRVPRLANAQDALQMMTTGQSLSGSRAKAMNLVHQVVEPDQLIPAAKQMIKDGLKPVAPWDEKGFKLPGGGIWTPAS
AQLWPAAPAILRRETSGNYPAALAILKCVYEGLQLPFDTGLKIEQRYFTEVLQTREAFSMIRSLFISMQELGKGARRPAG
IPKTELKHVGVVGAGFMGASIAYVTAAAGLAVTLIDRDMEAAAKGKAVCEGLVKDSVGKGRFTQDEAAALLARITPSANY
ADLAAAGLVIEAVFEDREVKKAVIEAVEAVLPEGAIFASNTSTLPISGLAKNSKRPADFIGIHFFSPVEKMMLTEVILGK
ATGDKALAVALDYVAAIKKTPIVVNDTRGFFVNRCVLRYMSESYDMLIEGVPPTMIENAAKMAGMPVGPLALNDEVAIDL
SLKILKATVADLGEEAIDPRHMDLISRMVEKEGRFGRKNSKGFYDYPPKPAKKSLWPELKTLYPQRRADEVDVNVLKQRF
LVTIALEAARTVEEGIVTDPREADVGSILGFGFAPYTGGALSYIDGMGAKAFVELAEKLAGTYGDHFKPTPLLKDMAAKG
ETFYGRFDPYAGAKVAA
>Mature_736_residues
TYINFTLETDADGIALVTWDMPGKSMNVFTSEVMAELDAIIDATTADAAVKGVVFTSGKSSFSGGADLSMIKSMFGSYQD
EKEKSPETAVQTLFGLVGRMSGLFRKLETSGKPWVSAINGTCMGGAFELSLACHGRVASNAKSVKIALPEVKVGIFPGAG
GTQRVPRLANAQDALQMMTTGQSLSGSRAKAMNLVHQVVEPDQLIPAAKQMIKDGLKPVAPWDEKGFKLPGGGIWTPASA
QLWPAAPAILRRETSGNYPAALAILKCVYEGLQLPFDTGLKIEQRYFTEVLQTREAFSMIRSLFISMQELGKGARRPAGI
PKTELKHVGVVGAGFMGASIAYVTAAAGLAVTLIDRDMEAAAKGKAVCEGLVKDSVGKGRFTQDEAAALLARITPSANYA
DLAAAGLVIEAVFEDREVKKAVIEAVEAVLPEGAIFASNTSTLPISGLAKNSKRPADFIGIHFFSPVEKMMLTEVILGKA
TGDKALAVALDYVAAIKKTPIVVNDTRGFFVNRCVLRYMSESYDMLIEGVPPTMIENAAKMAGMPVGPLALNDEVAIDLS
LKILKATVADLGEEAIDPRHMDLISRMVEKEGRFGRKNSKGFYDYPPKPAKKSLWPELKTLYPQRRADEVDVNVLKQRFL
VTIALEAARTVEEGIVTDPREADVGSILGFGFAPYTGGALSYIDGMGAKAFVELAEKLAGTYGDHFKPTPLLKDMAAKGE
TFYGRFDPYAGAKVAA

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=751, Percent_Identity=34.2210386151798, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI68989263, Length=747, Percent_Identity=27.3092369477912, Blast_Score=242, Evalue=8e-64,
Organism=Homo sapiens, GI261878539, Length=636, Percent_Identity=28.4591194968553, Blast_Score=221, Evalue=3e-57,
Organism=Homo sapiens, GI296179429, Length=293, Percent_Identity=35.4948805460751, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI296179427, Length=310, Percent_Identity=33.5483870967742, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI194097323, Length=199, Percent_Identity=27.6381909547739, Blast_Score=86, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=726, Percent_Identity=34.9862258953168, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1790281, Length=739, Percent_Identity=29.7699594046008, Blast_Score=299, Evalue=5e-82,
Organism=Escherichia coli, GI1787661, Length=287, Percent_Identity=33.1010452961672, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1787659, Length=200, Percent_Identity=27.5, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI221142681, Length=100, Percent_Identity=35, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=740, Percent_Identity=35.2702702702703, Blast_Score=358, Evalue=6e-99,
Organism=Caenorhabditis elegans, GI17508951, Length=740, Percent_Identity=35.2702702702703, Blast_Score=358, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI17558304, Length=741, Percent_Identity=32.5236167341431, Blast_Score=343, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI25144276, Length=616, Percent_Identity=35.8766233766234, Blast_Score=305, Evalue=6e-83,
Organism=Caenorhabditis elegans, GI71985923, Length=365, Percent_Identity=29.3150684931507, Blast_Score=162, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71985930, Length=368, Percent_Identity=26.0869565217391, Blast_Score=160, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17549919, Length=294, Percent_Identity=34.6938775510204, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553560, Length=289, Percent_Identity=32.1799307958478, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17563036, Length=292, Percent_Identity=31.5068493150685, Blast_Score=127, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17540714, Length=213, Percent_Identity=30.9859154929577, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=203, Percent_Identity=26.6009852216749, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=198, Percent_Identity=28.2828282828283, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=737, Percent_Identity=32.4287652645862, Blast_Score=316, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24583077, Length=737, Percent_Identity=32.4287652645862, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24583079, Length=737, Percent_Identity=32.4287652645862, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24653139, Length=284, Percent_Identity=27.8169014084507, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20129971, Length=210, Percent_Identity=27.1428571428571, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653477, Length=210, Percent_Identity=27.1428571428571, Blast_Score=92, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 78813; Mature: 78682

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYINFTLETDADGIALVTWDMPGKSMNVFTSEVMAELDAIIDATTADAAVKGVVFTSGK
CEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCC
SSFSGGADLSMIKSMFGSYQDEKEKSPETAVQTLFGLVGRMSGLFRKLETSGKPWVSAIN
CCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GTCMGGAFELSLACHGRVASNAKSVKIALPEVKVGIFPGAGGTQRVPRLANAQDALQMMT
CCCCCCEEEEEEEECCCHHCCCCEEEEECCCEEEEEECCCCCHHHCCCCCCHHHHHHHHH
TGQSLSGSRAKAMNLVHQVVEPDQLIPAAKQMIKDGLKPVAPWDEKGFKLPGGGIWTPAS
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AQLWPAAPAILRRETSGNYPAALAILKCVYEGLQLPFDTGLKIEQRYFTEVLQTREAFSM
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRSLFISMQELGKGARRPAGIPKTELKHVGVVGAGFMGASIAYVTAAAGLAVTLIDRDME
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEECCHHHHHHHHHHHHHCEEEEEECCCHH
AAAKGKAVCEGLVKDSVGKGRFTQDEAAALLARITPSANYADLAAAGLVIEAVFEDREVK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAVIEAVEAVLPEGAIFASNTSTLPISGLAKNSKRPADFIGIHFFSPVEKMMLTEVILGK
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHC
ATGDKALAVALDYVAAIKKTPIVVNDTRGFFVNRCVLRYMSESYDMLIEGVPPTMIENAA
CCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
KMAGMPVGPLALNDEVAIDLSLKILKATVADLGEEAIDPRHMDLISRMVEKEGRFGRKNS
HHCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
KGFYDYPPKPAKKSLWPELKTLYPQRRADEVDVNVLKQRFLVTIALEAARTVEEGIVTDP
CCCCCCCCCCCHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
READVGSILGFGFAPYTGGALSYIDGMGAKAFVELAEKLAGTYGDHFKPTPLLKDMAAKG
CCCCHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
ETFYGRFDPYAGAKVAA
CCEEECCCCCCCCCCCC
>Mature Secondary Structure 
TYINFTLETDADGIALVTWDMPGKSMNVFTSEVMAELDAIIDATTADAAVKGVVFTSGK
EEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHEEEEEECCC
SSFSGGADLSMIKSMFGSYQDEKEKSPETAVQTLFGLVGRMSGLFRKLETSGKPWVSAIN
CCCCCCCCHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GTCMGGAFELSLACHGRVASNAKSVKIALPEVKVGIFPGAGGTQRVPRLANAQDALQMMT
CCCCCCEEEEEEEECCCHHCCCCEEEEECCCEEEEEECCCCCHHHCCCCCCHHHHHHHHH
TGQSLSGSRAKAMNLVHQVVEPDQLIPAAKQMIKDGLKPVAPWDEKGFKLPGGGIWTPAS
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
AQLWPAAPAILRRETSGNYPAALAILKCVYEGLQLPFDTGLKIEQRYFTEVLQTREAFSM
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
IRSLFISMQELGKGARRPAGIPKTELKHVGVVGAGFMGASIAYVTAAAGLAVTLIDRDME
HHHHHHHHHHHCCCCCCCCCCCHHHHHHCCEEECCHHHHHHHHHHHHHCEEEEEECCCHH
AAAKGKAVCEGLVKDSVGKGRFTQDEAAALLARITPSANYADLAAAGLVIEAVFEDREVK
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KAVIEAVEAVLPEGAIFASNTSTLPISGLAKNSKRPADFIGIHFFSPVEKMMLTEVILGK
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHC
ATGDKALAVALDYVAAIKKTPIVVNDTRGFFVNRCVLRYMSESYDMLIEGVPPTMIENAA
CCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHH
KMAGMPVGPLALNDEVAIDLSLKILKATVADLGEEAIDPRHMDLISRMVEKEGRFGRKNS
HHCCCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
KGFYDYPPKPAKKSLWPELKTLYPQRRADEVDVNVLKQRFLVTIALEAARTVEEGIVTDP
CCCCCCCCCCCHHHHCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
READVGSILGFGFAPYTGGALSYIDGMGAKAFVELAEKLAGTYGDHFKPTPLLKDMAAKG
CCCCHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
ETFYGRFDPYAGAKVAA
CCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA