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The map label for this gene is artI [H]

Identifier: 209547795

GI number: 209547795

Start: 187652

End: 188527

Strand: Direct

Name: artI [H]

Synonym: Rleg2_0186

Alternate gene names: 209547795

Gene position: 187652-188527 (Clockwise)

Preceding gene: 209547793

Following gene: 209547801

Centisome position: 4.14

GC content: 62.21

Gene sequence:

>876_bases
ATGACGCAATCCAAGCTGGCATCCCTGACTCTCAAAGGGTTTTTCGCGTTCGGGCTGTTGCTGGCAGCGCTGTTTTGCGG
CTTTGCCGCATCCGCCCAGCAGGCAACTCCCTCGCTGCCGCTGCTTTTCGATGCGCGTGAACGTCTGGCGAGGCCCGATC
TCTCCTCGCTGGTTCGCTTGCGCTTCCTCACCTCGGTCGATTTTCCGCCGTTCAATTTCACCGATCAGAACGGCAAACTT
TCCGGTTTCAACGTCGATCTCGCCCGCGAAATCTGCAGTGAGCTGGAGATTTCCGACAAGTGCCAGATTCAGGCGATCCC
CTTCGCCGATCTCAAGGATGCGCTTGCCGCCTCGCAGGGTGATGCCGTGATTGCCGGCCTTGCCGTGACGCCGGAGCTGC
GCCGGCAATTCGTCTTCTCCCGGCCCTATCTGATGCTGCCGGCCCGCTTCGTGCGCAATCTCGCCGTGCCGCTCGACGGC
AAGACGGCTGCCGCGCTTTCGGCGCACCCGGTCGGTGTGGTCAGGGGCACGGTGCACGAGGCGATGCTGGCCGCCTTCTT
TCCGGCAATCAAGGCTCAGGCCTTCGACACCAAGGACGCTTTGCTTGCGGCACTCAAGGATCGCAAGGTCGATGCCGCCT
TCGCCGATGCGCTGCAGCTTTCCTTCTGGGTCTCGTCGCCGGCCTCGGCCAAATGCTGCGCCCTGTTCGACGGGCCCTAT
CTCTCCGAGCATTTCCTCGGCGAGGGCATGACGATCATGCTGCGGCAGAAGGACAGCGTGCTGACGTCGGCCATCGACCA
CGCGCTTGCCACGCTGTCGCGCAACGGCCGTCTCCAGGAAATCTATCTGCGCTATTTTCCCTACGGGCTTTATTGA

Upstream 100 bases:

>100_bases
ACGGTCATGCTCACCCCACCATATTCCCCGCCGGCCATGACTCTGTTACATCTTTCCGACCCCGCGATGCTTCGCCCTCA
ACCCTGAGACCAAGATCACG

Downstream 100 bases:

>100_bases
GCCTTCGAGCTTATCCCTTGCGGAAGCGGGCAATGGCGCTGCGCTCGATCGCCGCGCAGGTGAGCTTGTCGAGACCGAGC
CGGTCGCGCAGCAGGCTGAG

Product: family 3 extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTQSKLASLTLKGFFAFGLLLAALFCGFAASAQQATPSLPLLFDARERLARPDLSSLVRLRFLTSVDFPPFNFTDQNGKL
SGFNVDLAREICSELEISDKCQIQAIPFADLKDALAASQGDAVIAGLAVTPELRRQFVFSRPYLMLPARFVRNLAVPLDG
KTAAALSAHPVGVVRGTVHEAMLAAFFPAIKAQAFDTKDALLAALKDRKVDAAFADALQLSFWVSSPASAKCCALFDGPY
LSEHFLGEGMTIMLRQKDSVLTSAIDHALATLSRNGRLQEIYLRYFPYGLY

Sequences:

>Translated_291_residues
MTQSKLASLTLKGFFAFGLLLAALFCGFAASAQQATPSLPLLFDARERLARPDLSSLVRLRFLTSVDFPPFNFTDQNGKL
SGFNVDLAREICSELEISDKCQIQAIPFADLKDALAASQGDAVIAGLAVTPELRRQFVFSRPYLMLPARFVRNLAVPLDG
KTAAALSAHPVGVVRGTVHEAMLAAFFPAIKAQAFDTKDALLAALKDRKVDAAFADALQLSFWVSSPASAKCCALFDGPY
LSEHFLGEGMTIMLRQKDSVLTSAIDHALATLSRNGRLQEIYLRYFPYGLY
>Mature_290_residues
TQSKLASLTLKGFFAFGLLLAALFCGFAASAQQATPSLPLLFDARERLARPDLSSLVRLRFLTSVDFPPFNFTDQNGKLS
GFNVDLAREICSELEISDKCQIQAIPFADLKDALAASQGDAVIAGLAVTPELRRQFVFSRPYLMLPARFVRNLAVPLDGK
TAAALSAHPVGVVRGTVHEAMLAAFFPAIKAQAFDTKDALLAALKDRKVDAAFADALQLSFWVSSPASAKCCALFDGPYL
SEHFLGEGMTIMLRQKDSVLTSAIDHALATLSRNGRLQEIYLRYFPYGLY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787088, Length=270, Percent_Identity=27.7777777777778, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=26.9230769230769, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=26.0683760683761, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788648, Length=239, Percent_Identity=25.9414225941423, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=23.2876712328767, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 31677; Mature: 31546

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSKLASLTLKGFFAFGLLLAALFCGFAASAQQATPSLPLLFDARERLARPDLSSLVRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCHHHHCCCCHHHHHHH
RFLTSVDFPPFNFTDQNGKLSGFNVDLAREICSELEISDKCQIQAIPFADLKDALAASQG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCC
DAVIAGLAVTPELRRQFVFSRPYLMLPARFVRNLAVPLDGKTAAALSAHPVGVVRGTVHE
CEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHH
AMLAAFFPAIKAQAFDTKDALLAALKDRKVDAAFADALQLSFWVSSPASAKCCALFDGPY
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCEEEEECCCC
LSEHFLGEGMTIMLRQKDSVLTSAIDHALATLSRNGRLQEIYLRYFPYGLY
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQSKLASLTLKGFFAFGLLLAALFCGFAASAQQATPSLPLLFDARERLARPDLSSLVRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEEECCHHHHCCCCHHHHHHH
RFLTSVDFPPFNFTDQNGKLSGFNVDLAREICSELEISDKCQIQAIPFADLKDALAASQG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHCCCC
DAVIAGLAVTPELRRQFVFSRPYLMLPARFVRNLAVPLDGKTAAALSAHPVGVVRGTVHE
CEEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHH
AMLAAFFPAIKAQAFDTKDALLAALKDRKVDAAFADALQLSFWVSSPASAKCCALFDGPY
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHEEEECCCCCCCEEEEECCCC
LSEHFLGEGMTIMLRQKDSVLTSAIDHALATLSRNGRLQEIYLRYFPYGLY
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]